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41.
The changes during growth and sporulation in activities of cells of Bacillus subtilis to incorporate various amino acids were investigated with wild-type strain and its asporogenous mutant. In the case of wild type strain the uptake of valine, phenylalanine, and proline was largest during the logarithmic growth period. The uptake of these amino acids decreased rapidly during the early stationary phase. The uptake of valine and cysteine increased again to some extent just prior to the forespore stage. The uptake of glycine and serine, however, was largest at the forespore stage at which the formation of spore coat took place. From these observed phenomena it was assumed that the remarkable incorporation of glycine and serine into the wild type strain during sporulation was closely related to the formation of spore coat. 相似文献
42.
Shigeo Aibara Takahiro Inakuma Yuhei Morita 《Bioscience, biotechnology, and biochemistry》2013,77(11):3051-3052
Penicillium strains (n=394) preserved at NBRC (the NITE Biological Resource Center) were compared as to groupings (11 species-clusters) based on phylogeny and the production of bioactive compounds. The strains in two clusters, of which P. chrysogenum and P. citrinum are representative, showed higher rates of positive strains with multi-biological activities. 相似文献
43.
《Bioscience, biotechnology, and biochemistry》2013,77(6):1230-1237
Penicillum sp. 40, which can grow in an extremely acidic medium at pH 2.0 was screened from an acidic soil. This fungus produces xylanases when grown in a medium containing xylan as a sole carbon source. A major xylanase was purified from the culture supernatant of Penicillium sp. 40 and designated XynA. The molecular mass of XynA was estimated to be 25,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. XynA has an optimum pH at 2.0 and is stable in pH 2.0-5.0. Western blot analysis using anit-XynA antibody showed that XynA was induced by xylan and repressed by glucose. Also, its production was increased by an acidic medium. The gene encoding XynA (xynA) was isolated from the genomic library of Penicillium sp. 40. The structural part of xynA was found to be 721 bp. The nucleotide sequence of cDNA amplified by RT-PCR showed that the open reading frame of xynA was interrupted by a single intron which was 58 bp in size and encoded 221 amino acids. Direct N-terminal amino acid sequencing showed that the precursor of XynA had a signal peptide composed of 31 amino acids. The molecular mass caliculated from the deduced amino acid sequence of XynA is 20,713. This is lower than that estimated by gel electrophoresis, suggesting that XynA is a glycoprotein. The predicted amino acid sequence of XynA has strong similarity to other family11 xylanases from fungi. 相似文献
44.
《Bioscience, biotechnology, and biochemistry》2013,77(10):1732-1734
A new oleanane triterpene was isolated from okara fermented with Penicillium simplicissimum ATCC 90288. Its structure was established to be 7β, 15α,24-trihydroxyolean-12-en-3,11,22-trione by spectroscopic techniques and an X-ray crystaliographic analysis. 相似文献
45.
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47.
Xia Wei Chan Feng Xiao‐Hui Li Xing‐Xin Mao Hai‐Bin Luo Dong‐Mei Zhang Li Rong Zhen‐Yu Xie Xin Yu Jie Li Wen‐Cai Ye Xiao‐Jun Huang Cui‐Xian Zhang 《化学与生物多样性》2019,16(6)
One new racemic mixture, penicilliode A ( 1 ) and four pairs of enantiomeric polyketides, penicilliode B and C ( 2 and 3 ) and coniochaetone B and C ( 4 and 5 ), were obtained from the starfish‐derived symbiotic fungus Penicillium sp. GGF16‐1‐2. Interestingly, the strain GGF16‐1‐2 can produce enantiomers. The absolute configuration of 1 was determined by X‐ray diffraction (XRD) analysis, and the absolute configurations of 2 – 4 were determined by the optical rotation (OR) values and electronic circular dichroism (ECD) calculations. Compounds 1 – 5 were firstly isolated from the marine‐derived fungus Penicillium as racemates, and 2 – 5 were separated by HPLC with a chiral stationary phase. All the compounds were evaluated for their antibacterial, cytotoxic and inhibitory activities against PDE4D2. 相似文献
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49.
Abstract: Fungal pellets (diameters ranging from 0.5 to 2 mm) were precultured in sugar-containing industrial sewage. They were packed into chromatography columns and then tested for biosorption of silver, copper and lead, using the downflow method. The physical parameters taken (differential pressure at the column, flow rate, bed height) showed rather good mechanical properties of the pellets. Charging the column with heavy metal solution (1 mM Ag+, Cu2+ or Pb2+ as nitrates in distilled water) resulted in very good biosorptive properties. Eluted solution contained less than or an equal amount of 1 gM heavy metal, demonstrating a removal of more than 99.9% of added metal. 相似文献
50.
Penicillium canescens SBUG-M 1139 was shown to be able to grow using phenoxybutyric acid as the sole carbon source. The rapid conversion of the
phenoxyalkanoic acid resulted in the formation of phenol, which was metabolized completely. These reactions were accompanied
by an accumulation of the methyl ketone phenoxypropan-2-one. Furthermore, during the metabolism of phenoxybutyric acid, 4-phenoxy-2,3-dehydrobutyric
acid, 4-phenoxy-3-hydroxybutyric acid, phenoxyacetic acid, and phenoxypropan-2-ol accumulated in minor amounts. Clearly, fungi
can metabolize phenoxyalkanoic acids to produce methyl ketones in a manner analogous to that used for the conversion of short-
or medium-chain fatty acids by fungi.
Received: 7 May 1999 / Accepted: 23 August 1999 相似文献