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11.
Size selectivity of aqueous pores in stomatous cuticles of <Emphasis Type="Italic">Vicia faba</Emphasis> leaves 总被引:1,自引:0,他引:1
Size selectivity of aqueous pores in Vicia leaf cuticles was investigated by measuring the penetration of calcium salts into the abaxial surface of detached leaves. Molecular weights of salts ranged from 111 g mol–1 to 755 g mol–1. Penetration in light at 20°C and 100% humidity was a first order process and rate constants of penetration ranged from 0.39 h–1 (CaCl2) to 0.058 h–1 (Ca-lactobionate). Penetration was a first order process in the dark as well, but the rate constants were smaller by a factor of 1.82. Plotting logarithmatised rate constants versus anhydrous molecular weights resulted in straight lines both in light and in the dark. The slopes per hour were very similar and the average slope was –1.2×10–3 mol g–1. Hence, size selectivity was not affected by stomatal opening, and in light or darkness permeability of Vicia cuticles decreased by a factor of 2.9 when molecular weight increased from 100 g mol–1 to 500 g mol–1. Silver nitrate was preferentially precipitated as silver chloride in guard cells, glandular trichomes and at the base of trichomes. It was concluded that these precipitates mark the location of aqueous pores in Vicia leaf cuticles. The size selectivity of aqueous pores in Vicia leaf cuticles is small compared to that observed in poplar leaf cuticles, in which permeability decreased by a factor of 7–13 for the same range of molecular weights. It is also much smaller than size selectivity of the lipophilic pathway in cuticles. These findings suggest that active ingredients of pesticides, growth regulators and chemical inducers with high molecular weights penetrate leaves at higher rates when formulated as ions. 相似文献
12.
Czerwinski SE Skvorak JP Maxwell DM Lenz DE Baskin SI 《Journal of biochemical and molecular toxicology》2006,20(5):241-246
Knowledge of partition coefficient (log P) data can play a critical role in understanding the pharmacokinetic and pharmacodistributive properties of toxic organophosphorus (OP) compounds. Using a recently published gas chromatographic method, the octanol:water log P values for the compounds tabun (GA), sarin (GB), cyclosarin (GF), and O-ethyl-S-(2-diisopropylaminoethyl) methylphosphonothiolate (VX) were determined to be 0.384 +/- 0.033, 0.299 +/- 0.016, 1.038 +/- 0.055, and 0.675 +/- 0.070, respectively. Based on these data, the log P value of the fluorophosphonate fragment, common to GB, soman (GD), and GF, was determined to be -2.256 +/- 0.273. The predictive value for absorption and distribution of the determined log P values was compared to measured values. The time to onset of local fasciculations (47.3, 29.0, 8.8, 8.5, and 6.3 min, respectively) in guinea pigs exposed percutaneously to equilethal doses of GA, VX, GF, GB, or GD was used as an indicator of dermal penetration. There was a good correlation (r = 0.95) between the measured log P value and the rate of onset of local fasciculations. Assuming a direct correspondence, equilibrium tissue:blood log P may be estimated from octanol:water log P. Comparison of the estimated and directly measured tissue:blood log P revealed a correlation of 0.8 for GD in liver, muscle, and adipose tissue. Our results demonstrate the use of log P data to both predict absorption and determine the distribution of OP compounds in tissues. This facilitates further estimates of in vivo OP effects from in vitro experiments. 相似文献
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14.
Greta Jarockyte Dominyka Dapkute Vitalijus Karabanovas Justinas V. Daugmaudis Feliksas Ivanauskas Ricardas Rotomskis 《Biochimica et Biophysica Acta (BBA)/General Subjects》2018,1862(4):914-923
Background
Monolayer cell cultures have been considered the most suitable technique for in vivo cellular experiments. However, a lot of cellular functions and responses that are present in natural tissues are lost in two-dimensional cell cultures. In this context, nanoparticle accumulation data presented in literature are often not accurate enough to predict behavior of nanoparticles in vivo. Cellular spheroids show a higher degree of morphological and functional similarity to the tissues.Methods
Accumulation and distribution of carboxylated CdSe/ZnS quantum dots (QDs), chosen as model nanoparticles, was investigated in cellular spheroids composed of different phenotype mammalian cells. The findings were compared with the results obtained in in vivo experiments with human tumor xenografts in immunodeficient mice. The diffusive transport model was used for theoretical nanoparticles distribution estimation.Results
QDs were accumulated only in cells, which were localized in the periphery of cellular spheroids. CdSe/ZnS QDs were shown to be stable and inert; they did not have any side-effects for cellular spheroids formation. Penetration of QDs in both cellular spheroids and in vivo tumor model was limited. The mathematical model confirmed the experimental results: nanoparticles penetrated only 25 μm into cellular spheroids after 24 h of incubation.Conclusions
Penetration of negatively charged nanoparticles is limited not only in tumor tissue, but also in cellular spheroids.General Significance
The results presented in this paper show the superior applicability of cellular spheroids to cell monolayers in the studies of the antitumor effect and penetration of nanomedicines. 相似文献15.
Pinewood nematodes (Bursaphelenchus xylophilus) and Asian longhorned beetles (Anoplophora glabripennis) are the primary regulated pests for packaging wood and timber in the EU, while the house longhorned beetle (Hylotrupes bajulus) is the most important cosmopolitan pest of construction wood. Gaseous hydrogen cyanide (HCN) is one of the few fumigation alternatives to the banned ozone-depleting chemical methyl bromide (MBr). This study reports the results of HCN fumigation experiments in a hermetically sealed steel chamber regarding (1) the penetration and absorption rates of HCN in wooden blocks, and (2) the biological efficacy of HCN against the wood-infesting pests B. xylophilus (in sawdust), A. glabripennis, and H. bajulus (in wooden blocks). A concentration equilibrium for HCN (at 20 g m−3) between the fumigation chamber headspace and the center of the treated spruce blocks (100 × 100 × 120 mm) was reached after 48 h in the saturated atmosphere. A dose of 10 g m−3 in the center of the spruce blocks was reached for both saturated and non-saturated atmospheres after 24 h of fumigation. The wood tested absorbed approximately 40–45% of the HCN, until equilibrium was reached. The highest tested HCN dose (20 g m−3) led to 100% mortality of the A. glabripennis and H. bajulus larvae after less than 1 h of exposure. For 20 g m−3 and 1 h exposure the Ct product was <18.66 g*h/m3 for H. bajulus and <17.67 g*h/m3 for A. glabripennis. Hydrogen cyanide doses of 10 g m−3 and 20 g m−3 led to 100% B. xylophilus mortality in 40 and 18 h, respectively. For B. xylophilus the Ct product was <424.00 g*h/m3 for 20 °C and 10 g m−3 and <349.51 g*h/m3 for 25 °C and 20 g m−3. The initial results are promising in terms of establishing an alternative technology and protocol to MBr for timber fumigation. 相似文献
16.
Lianhu Zhang Yan Cai Yunxi Li Tian Zhang Baohua Wang Guodong Lu Dongmei Zhang Stefan Olsson Zonghua Wang 《Molecular Plant Pathology》2021,22(9):1159-1164
Polar growth during appressorium formation is vital for the penetration peg formation in the rice blast fungus, Magnaporthe oryzae. Previous research has shown that the Sln1-septin-exocyst complex, localized at the base of the appressorium in contact with the leaf surface, forms a ring structure that influences growth polarity and affects penetration peg formation, and is necessary for pathogenicity. Our previous research showed CK2 proteins assemble another ring structure positioned perpendicular to the Sln1-septin-exocyst complex. Our research showed that the CK2 ring needs to become correctly assembled for penetration peg function and subsequent plant infection. In the present study, we found that the ring structures of CK2 are absent in the appressorium of ΔMoSep3 septin deletion mutants lacking the septin ring of the Sln1-septin-exocyst complex. Sln1 affects the septin proteins that recruit the exocyst complex that localizes as another ring at the appressorium's bottom. Destruction of the exocyst complex by mutation also causes incorrect localization of the CK2 ring structure. In conclusion, CK2 probably takes part in reestablishing the appressorium' spolarity growth necessary for penetration peg formation. We can also conclude that the correct localization and assembly of one or more CK2 ring structures in the appressorium depend on the initial assembly of the Sln1-septin-exocyst complex two rings at the base of the appressorium. 相似文献
17.
目的:通过研究不同促透剂对吲哚关辛水凝胶贴剂透皮性能的影响,遴选在特定栽药剂量时具有最佳促透效果的促透剂,并与市售贴剂进行比较,对吲哚美辛水凝胶贴剂的体外透皮性能进行评价。方法:采用改良Franz透皮扩散池,以离体小鼠背部皮肤为透皮屏障,在最佳载药量选用不同浓度的氮酮、油酸、丙二醇以及三者组成的二元或三元组合为促透剂,在规定时间点测定吲哚美辛的累积透过百分率以及单位面积累积透过量。结果:与空白对照组相比,当氮酮与油酸单独应用时,二者均没有明显的促透作用;当选用二元促透剂联合应用时,油酸与丙二醇联用能够明显促进吲哚美辛的经皮渗透(P〈0.05);当选用三元促透剂时促透效果更好,单位面积累积透过量最高可达234.4μg·cm^-2,24h内药物累积透过百分率明显高于市售贴剂。结论:氮酮、油酸、丙二醇三者联合应用可作为吲哚关辛贴剂的理想促透剂。吲哚关辛水凝胶贴剂是具有应用价值的新型经皮控释制剂。 相似文献
18.
19.
离子导入法和渗透促进剂并用对裸鼠皮肤角质层影响的ESR研究 总被引:8,自引:0,他引:8
通过测定5-唑烷氮氧自由基硬脂酸(5-DSA)标记裸鼠皮肤角质层的ESR谱,研究离子导入法与渗透促进剂100%月桂氮酮(100%AZ)、5%月桂氨 酮/丙二醇溶液(5% AZ/PG)和10%油酸/丙二醇溶液(10%OA/PG)并用对裸鼠皮肤角质层的影响。离子导入法处理皮肤后,标记物序参数降低,各向同性超精细分裂偶合常数增大,表明低密度电流能够引起皮肤角质层细胞间脂质排列有序性降低,流动性增大,极性增大;离子导入法与渗透促进剂并用处理皮肤后,序参数进一步降低,各向同性超精细分裂偶合常数进一步增大,表明二者对皮肤角质层的影响具有协同作用。 相似文献
20.
《Nucleosides, nucleotides & nucleic acids》2013,32(5-7):1059-1061
A method was developed for the synthesis of oligonucleotide-cationic peptide conjugates in solution phase by disulfide bond formation. Precipitation was avoided by the easily removable triethylammonium trifluoroacetate (TEATFAc) salt which served at the same time as a buffer of the reaction mixture. The fast and high yielding disulfide bond formation was due to the Npys thio protecting and activating group of Cys. A solution of the free 5′-thiol modified oligonucleotide obtained from Poly-Pak? purification was used for conjugation. 相似文献