Original Articles: Dynamic Redistribution of Isotopically Labeled Cohorts of Nitrogen Inputs in Two Temperate Forests

We compared simulated time series of nitrogen-15 (¹⁵N) redistribution following a large-scale labeling experiment against field recoveries of ¹⁵NH₄ ⁺ and ¹⁵NO₃ ⁻ in vegetation tissues. We sought to gain insight into the altered modes of N cycling under long-term, experimentally elevated N inputs. The study took place in two contrasting forests: a red pine stand and a mixed deciduous stand (predominantly oak) at the Harvard Forest, Massachusetts, USA. We used TRACE, a dynamic simulation model of ecosystem biogeochemistry that includes ¹⁵N/¹⁴N ratios in N pools and fluxes. We simulated input–output and internal fluxes of N, tracing the labeled cohorts of N inputs through ecosystem pools for one decade. TRACE simulated the peaks and timing of ¹⁵N recovery in foliage well, providing a key link between modeling and field studies. Recovery of tracers in fine roots was captured less well. The model was structured to provide rapid, initial sinks for ¹⁵NO₃ ⁻ and ¹⁵NH₄ ⁺ in both forests, as indicated by field data. In simulations, N in litter turned over rapidly, even as humus provided a long-term sink for rapidly cycling N. This sink was greater in the oak forest. Plant uptake fluxes of N in these fertilized plots were on the same order of magnitude as net assimilation fluxes in forest-floor humus. A striking result was the small rate of incorporation of N in humus resulting from the transfer of litter material to humus, compared with large fluxes of N into humus and its associated microorganisms through direct transfers from pools of inorganic N in soils. Received 19 May 1998; accepted 30 September 1998     

哀牢山湿性常绿阔叶林林冠和林地腐殖质理化特性、微生物量及酶活性比较

在热带、亚热带和温带高海拔潮湿生境的山地森林林冠层中,积累有较为丰富的林冠腐殖质(Canopy humus),是构成山地森林生态系统景观结构的重要组分,为丰富的附生植物提供了重要的生长基质和营养物质.通过对云南哀牢山山地湿性常绿阔叶林林冠腐殖质和其相应林下地表腐殖质的分析测定结果表明,由于林冠和林下地表腐殖质的来源、组成和空间分布的不同,它们之间的理化特性存在较大的差异,其中林冠腐殖质中有机C、全N及全Ca的含量、C/N以及阳离子交换量显著高于林下地表腐殖质,而全K和全Mg的含量则显著低于后者,全P含量差异不明显;林冠腐殖质的微生物量C、N和呼吸强度,以及蔗糖酶、脲酶和蛋白酶的活性均显著高于林下地表腐殖质,说明林冠腐殖质是一种具有较高生物活性的有机土类物质,在山地森林生态系统养分循环、林冠附生植物多样性格局形成及其维持方面具有重要的作用.     

Impact of floor management practices on the growth of groundcover species and soil properties in an apple orchard in northern China

Tillage and groundcover are the two mainly used management practices in orchard. Only small portion of orchard has been treated with groundcover in China, which would restrict our understanding of the scientific management of apple orchard. This study aimed to investigate the impact of different groundcover treatments on the plant growth of groundcover species and soil properties in an apple orchard near Beijing, northern China. Six commonly used groundcover species were chosen to grow underground. Our results showed that groundcover species had significant greater maximum photosynthesis rate (P_max) than weeds in control plots. Meanwhile, groundcover treatments could largely improve the microclimate of orchard compared with tillage treatment. Among all the groundcover species, alfalfa was proven to be the most appropriate species grown underground in apple orchard for its high P_max and high above-ground biomass. Groundcover treatments had little impact on soil bulk density, soil porosity and surface soil organic carbon (SOC) content. However, significantly greater deep soil organic carbon contents were found in two grass species, and significantly higher soil available nitrogen (N) contents were found in two leguminous species respectively. Our results indicated that groundcover treatment would be a sustainable management practice for apple orchard in northern China.     

浙江天童常绿阔叶林不同演替阶段地表凋落物的C:N:P化学计量特征

地表凋落物在森林物质循环中起着重要作用, 但是目前缺乏对其不同分解层次中碳(C)、氮(N)、磷(P)演替动态的研究。该文以浙江天童常绿阔叶林为研究对象, 用空间代替时间序列的方法, 通过测定5个演替阶段地表凋落物不同分解层次的凋落物量、有机碳库和氮磷养分库的储量及C:N:P化学计量特征, 探讨地表凋落物特征的演替动态。结果表明: 1)随着演替的进行, 地表凋落物量和有机碳储量呈现下降的趋势。2)在各演替阶段, 有机碳含量在各分解层表现出未分解层(L) > 半分解层(F) > 已分解层(Y)的趋势; 有机碳储量均表现为Y < F。3)演替前期群落氮含量和储量显著低于演替中后期群落; 不同分解层的氮含量在各演替阶段皆表现为: Y > F > L, 且各层氮含量随着演替的进行均趋于升高。4)磷含量在演替中期群落最低, 各演替阶段不同分解层的磷含量皆表现为Y > F > L。磷储量的演替趋势不明显。L层磷储量随着演替进行趋于降低。5)随着演替进行, 凋落物C:N、C:P和N:P皆趋于下降(p < 0.05)。在各分解层之间, C:N和C:P皆表现为Y < F < L, N:P差异不显著。总之, 随着演替进行, 天童常绿阔叶林地表凋落物量降低, 有机碳库及氮磷养分库的含量趋于升高, 储量趋向降低, C:N:P趋于下降, 体现了生态系统碳和养分循环随着演替进行在不断优化。     

Population structure and dynamics of the clonal dwarf-shrub Linnaea borealis

Abstract. The demography of the long-lived clonal dwarf-shrub Linnaea borealis was studied during four years in a coniferous forest in central Sweden. The main object was to infer patterns of temporal variation in population dynamics of this species. The shoot population is organized in fragments, i.e. physically connected systems of shoots partly covered by the moss carpet. The age and size structure of the fragment population is described, but shoots are more convenient units for a study of population dynamics. A stochastic model of shoot population dynamics was constructed, and simulations indicated a considerable temporal variation in population size and flowering. Hence, variability as such is an essential aspect of the dynamics of established populations of Linnaea. Simulations of extinction risks revealed that small-sized shoot populations (ca. 250 shoots) are likely to be long-lived when experiencing environmentally induced demographic variation of the range observed. Mortality agents for established genets, such as large-scale disturbances, were not incorporated in the models. Some implications of variable population growth rates in clonal plants in woodlands are discussed.     

Evaluating residual strain throughout the murine female reproductive system

Mounting evidence suggests that cells within soft tissues seek to maintain a preferred biomechanical state. Residual stress is defined as the stress that remains in a tissue when all external loads are removed and contributes to tissue mechanohomeostasis by decreasing the transmural gradient of wall stress. Current computational models of pelvic floor mechanics, however, often do not consider residual stress. Residual strain, a result of residual stress can be quantitatively measured through opening angle experiments. Therefore, the objective of this study is to quantify the regional variations in opening angles along the murine female reproductive system at estrus and diestrus, to quantify residual strain in the maintenance state of sexually mature females. Further, evidence suggests that hydrophilic glycosaminoglycan/proteoglycans are integral to cervical remodeling. Thus, variations in opening angles following hypo-osmotic loading are evaluated. Opening angle experiments were performed along the murine reproductive system in estrus (n = 8) and diestrus (n = 8) and placed in hypo-osmotic solution. Measurements of thickness and volume were also obtained for each group. Differences (p < 0.05) in opening angle were observed with respect to region and loading, however, differences with respect to estrous stage were not significant. Thickness values were significant (p < 0.05) with respect to region only. The effects of both estrous cycle and region resulted in significant differences (p < 0.05) in observed volume. The observed regional differences indicate variation in the stress-free state among the reproductive system which may have implications for future computational models to advance women’s reproductive health.     

低位微生物发酵床养猪舍的设计与应用

新型低位微生物发酵床是在传统漏缝地板的下方建设发酵床,由漏缝地板与发酵床结合形成。低位微生物发酵床利用谷壳、锯糠、椰糠做垫料,加入微生物发酵剂,混合搅拌,平铺在发酵床内。发酵床表面建立一个轨道运行的翻堆蛟龙,定期翻堆混合垫料。猪群排泄物通过人工收集或经漏缝地板漏到发酵床上,经微生物发酵,消除臭味,分解粪污,发酵产生有机肥,达到养猪污染治理的目的。     

Life Cycle Assessment of Wood Floor Coverings - A Representative Study for the German Flooring Industry (11 pp)

Goal, Scope and Background The goal of the study is a life cycle assessment according to ISO 14040 –14043 for wood floor coverings (solid parquet, multilayer parquet, solid floor board and wood blocks). The representative study covers approximately 70% of all wood flooring production in Germany. The comparison of the floor coverings among each other was not the aim. Instead the study provides basic data for all wood floor coverings for a possible comparison with other floor coverings later on. The main focus was a hot spot analysis to help the involved industry partners to improve their environmental performance, and to use the results for marketing purposes. - Inventory Analysis. The study covers the whole life cycle from forest management, sawmilling, manufacturing, laying and surface finishing through to refurbishment and end-of-life. The end-of-life scenario is the thermal utilisation of the floor coverings. The energy gained in the end-of-life scenario is accounted for by system expansion (avoided burden approach). - Impact Assessment. In the Impact Assessment the following categories were considered: global warming (GWP), acidification (AP), eutrophication (EP), ozone depletion (ODP) and photo-oxidant formation (POCP) following the CML baseline 2000 method. Furthermore the use of primary energy is presented. The low emissions of greenhouse gases during the life cycle can lead to a negative contribution to the global warming potential if more emissions are avoided through the substitution process than are emitted during the life cycle of the product. Mainly energy consumption and the use of solvents influence the environmental impacts of the systems under analysis. The most relevant unit processes for the issue of energy consumption are 'production' and for photo-oxidant formation 'laying', 'surface finishing' and 'refurbishment'. These are therefore the unit processes with the greatest potential for improvement. - Normalisation and Sensitivity Analysis. The normalisation results show that the photo-oxidant formation potential is most significant in comparison to the other impact categories. Improvement options and the choice of the functional unit have been further explored in a sensitivity analysis. Discussion and Conclusions. The most important opportunities for improvements are located in the unit processes laying, surface finishing and refurbishment. The POCP result can be reduced significantly depending on the choice of glue and varnish at each of these stages. The results of the sensitivity analysis showed a potential for improvement in this category. No data for the production of an oil and wax finish was available. This option would be interesting to consider at in a further study. The time aspect of storing CO2 for a period of time is not considered in this paper, but will be addressed in a forthcoming paper (Nebel and Cowell 2003).     

A Prediction Model Based on Biomarkers and Clinical Characteristics for Detection of Lung Cancer in Pulmonary Nodules

Lung cancer early detection by low-dose computed tomography (LDCT) can reduce the mortality. However, LDCT increases the number of indeterminate pulmonary nodules (PNs), whereas 95% of the PNs are ultimately false positives. Modalities for specifically distinguishing between malignant and benign PNs are urgently needed. We previously identified a panel of peripheral blood mononucleated cell (PBMC)-miRNA (miRs-19b-3p and -29b-3p) biomarkers for lung cancer. This study aimed to evaluate efficacy of integrating biomarkers and clinical and radiological characteristics of smokers for differentiating malignant from benign PNs. We analyzed expression of 2 miRNAs (miRs-19b-3p and -29b-3p) in PBMCs of a training set of 137 individuals with PNs. We used multivariate logistic regression analysis to develop a prediction model based on the biomarkers, radiographic features of PNs, and clinical characteristics of smokers for identifying malignant PNs. The performance of the prediction model was validated in a testing set of 111 subjects with PNs. A prediction model comprising the two biomarkers, spiculation of PNs and smoking pack-year, was developed that had 0.91 area under the curve of the receiver operating characteristic for distinguishing malignant from benign PNs. The prediction model yielded higher sensitivity (80.3% vs 72.6%) and specificity (89.4% vs 81.9%) compared with the biomarkers used alone (all P < .05). The performance of the prediction model for malignant PNs was confirmed in the validation set. We have for the first time demonstrated that the integration of biomarkers and clinical and radiological characteristics could efficiently identify lung cancer among indeterminate PNs.