全文获取类型
收费全文 | 86283篇 |
免费 | 3510篇 |
国内免费 | 7699篇 |
出版年
2023年 | 748篇 |
2022年 | 1180篇 |
2021年 | 1535篇 |
2020年 | 1606篇 |
2019年 | 2947篇 |
2018年 | 1926篇 |
2017年 | 1613篇 |
2016年 | 2028篇 |
2015年 | 3219篇 |
2014年 | 4257篇 |
2013年 | 5767篇 |
2012年 | 3661篇 |
2011年 | 5062篇 |
2010年 | 3671篇 |
2009年 | 3820篇 |
2008年 | 4090篇 |
2007年 | 4366篇 |
2006年 | 4038篇 |
2005年 | 3477篇 |
2004年 | 2928篇 |
2003年 | 2615篇 |
2002年 | 2315篇 |
2001年 | 1894篇 |
2000年 | 1727篇 |
1999年 | 1694篇 |
1998年 | 1601篇 |
1997年 | 1354篇 |
1996年 | 1237篇 |
1995年 | 1444篇 |
1994年 | 1374篇 |
1993年 | 1340篇 |
1992年 | 1321篇 |
1991年 | 1097篇 |
1990年 | 1049篇 |
1989年 | 939篇 |
1988年 | 930篇 |
1987年 | 858篇 |
1986年 | 620篇 |
1985年 | 1065篇 |
1984年 | 1445篇 |
1983年 | 982篇 |
1982年 | 1369篇 |
1981年 | 971篇 |
1980年 | 974篇 |
1979年 | 904篇 |
1978年 | 526篇 |
1977年 | 432篇 |
1976年 | 350篇 |
1975年 | 276篇 |
1973年 | 274篇 |
排序方式: 共有10000条查询结果,搜索用时 962 毫秒
961.
Prof. Rita Pascolini P. Ceccarelli A. M. Gargiulo S. Lorvik 《Cell and tissue research》1985,239(2):443-445
Summary In the testis of Esox lucius at the time of spermiation, activity of cyclic adenosine 3,5-monophosphate (cAMP) was immunocytochemically localized at the level of the Sertoli cells. In these cells adenylate cyclase activity was also ultracytochemically demonstrated by using adenylyl imidodiphosphate as a substrate. Reaction products of adenylate cyclase were primarily detectable on the basal and adluminal plasma membranes and on the surface of protrusions of the cell body into the lumen. 相似文献
962.
Summary At the antennal tip of the collembolan insect Hypogastrura socialis two terminal-pore sensilla are located, which, in addition to normally structured and most probably chemosensitive sensory cells, also contain aberrant sensory cells. Portions of these cells resemble chemoreceptors but also shown are features that, as a rule, occur in mechanoreceptors. One cell in each sensillum is remarkable in two characteristics: (1) Its dendritic outer segment does not reach the cuticular outer structures of the sensillum; (2) it contains dense-core granules (diameter 60–110 nm) within its perikaryon, its dendritic inner segment and its axon. Additionally, these two cells do not show lengthening of their dendritic outer segment during molt as do all other sensory cells. Among the fibers of one major branch of the antennal nerve within the head capsule a single axon was observed to contain dense-core granules. This axon was traced to its termination where normal synaptical contacts were found. Based on the assumption that the axon belongs to one of the granule-containing sensory cells two alternative hypotheses are proposed: (1) an individual sensory cell of a sensillum may synthesize a transmitter that is different from that of the other sensory cells of this sensillum; (2) the aberrant cells have lost exteroceptive functions but act as neuromodulatorsSupported by the Deutsche Forschungsgemeinschaft (SFB 4/G1) 相似文献
963.
Subcellular localization and glycoprotein nature of the invertase from the fission yeast Schizosaccharomyces pombe 总被引:6,自引:0,他引:6
Sergio Moreno Teresa Ruíz Yolanda Sánchez Julio R. Villanueva Luís Rodríguez 《Archives of microbiology》1985,142(4):370-374
The subcellular localization of the enzyme invertase in Schizosaccharomyces pombe cells, both repressed and derepressed for synthesis of the enzyme, was studied. Most of the invertase was found to be located outside the plasma membrane and only a small percentage was found to be associated to membranes. A substantial portion of the external enzyme remained firmly bound to cell-wall material.All of the invertase recovered in soluble form from cellular extracts reacted with concanavalin A and with the lectin from Bandeiraea simplicifolia seeds, indicating the presence in the enzyme of a carbohydrate moiety which probably contains terminal mannosyl (or structurally related) and galactosyl residues.The possibility of the presence of two different forms of invertase in S. pombe was considered. An intracellular, soluble form of invertase, devoid of carbohydrate, similar to the small invertase of the budding yeast Saccharomyces cerevisiae, was not found in S. pombe. However, the Michaelis constant for sucrose of the enzyme present in repressed cells was smaller than that of the invertase synthesized under derepressing conditions, although this difference could also be the result of a different pattern of glycosylation of the invertase synthesized under different growth conditions. 相似文献
964.
Auxin action: the search for the receptor 总被引:2,自引:1,他引:1
J. W. CROSS 《Plant, cell & environment》1985,8(5):351-359
Abstract. The molecular specificity of the substances which have auxin activity implies the existence of specific receptors. There have been many efforts to identify and isolate these receptors on the assumption that they should bind auxins with affinities coordinate to their activities in bioassays. However, the known complexity of auxin uptake and metabolism make this assumption seriously deficient. Although several such binding sites have, in fact, been identified, proof of a connection between these sites and auxin action has been lacking. Definite proof would include a requirement that the site be reconstituted, together with the appropriate macro-molecular machinery, to construct a model of an auxin response. At the moment, our ignorance of the biochemistry and molecular biology of auxin growth responses makes such a proof difficult. However, two avenues of research promise to accelerate the rate of progress. The increasingly potent tools of molecular biology should soon allow the dissection of auxin-regulated gene expression, while improved knowledge of plasma membrane proton pumps and the mechanism of cell wall biosynthesis should produce, in parallel, an understanding of the auxin regulation of acid growth. 相似文献
965.
Abstract Chenopodium album L. plants, grown under controlled environmental conditions on different levels of soil nitrate, produced seeds with proportionately different NO?3 contents. Regardless of the endogenous NO?3 content, few seeds germinated in water or upon treatment with KNO3. Ethylene promoted germination, and the extent of germination was positively correlated with the endogenous seed NO?3 content. Combined application of ethylene and KNO3 in the dark had a synergistic effect on NO?3 -deficient seed. The synergism between ethylene and KNO3 was attributable to the NO?3 moiety of the nitrate salt. Ethylene and light showed moderate synergism in seeds with low or high endogenous nitrate. Addition of nitrate, however, masked the interaction between ethylene and light. Gibberellic acid4+7 (GA4+7) or red light, each alone or combined with KNO3, had little effect on germination. When applied together in the dark, ethylene and GA4+7 synergistically enhanced the germination of NO?3-deficient seed. The combined effects of the two hormones on this seed were further enhanced by the addition of KNO3. There was no synergism between ethylene and GA4+7 in NO?3-rich seed. These interactions among GA4+7, ethylene and KNO3 were not affected by light. The results confirm and further elaborate our earlier finding that the sensitivity of C. album seeds to ethylene may depend on nitrate availability. 相似文献
966.
Restriction endonuclease EcoO109 from Escherichia coli H709c with heptanucleotide recognition site 5'-PuG/GNCCPy 总被引:1,自引:0,他引:1
A new restriction endonuclease, EcoO109, has been isolated from Escherichia coli H709c by polyethyleneimine (PEI) precipitation, DEAE-cellulose chromatography and heparin agarose chromatography. The yield was high, more than 3000 units/g of wet cells. The EcoO109 endonuclease recognizes and cleaves a nucleotide sequence of (formula: see text), in the presence of 10 mM Mg2+. The enzyme will be useful for structural analysis and molecular cloning of DNA because of the stability, high yield and easy handling of the producer strain. 相似文献
967.
968.
The pyr4 gene of Neurospora crassa, which codes for orotidine-5'-phosphate decarboxylase, is capable of transforming an Aspergillus nidulans pyrG mutant by chromosomal integration, despite low homology between the transforming DNA and the recipient genome. Integration of pFB6, a plasmid carrying pyr4 and capable of replication in Escherichia coli, was not observed at the pyrG locus. The efficiency of transformation was considerably enhanced (50-100 fold) by inclusion in the transforming vector of a 3.5-kb A.nidulans chromosomal sequence, ans1. Although this sequence was isolated on the basis of replicating activity in Saccharomyces cerevisiae, there was no evidence for such activity in A.nidulans. Part of the ans1 fragment appears to be reiterated in the A.nidulans genome, though it is not yet clear whether this is directly responsible for the high transformation frequency. The efficiency of transformation of A.nidulans by plasmids bearing ans1, using an improved protocol, was approx. 5 X 10(3) stable transformants per microgram of plasmid DNA. 相似文献
969.
970.
Nonrandom insertion of Tn5 into cloned human adenovirus DNA 总被引:4,自引:0,他引:4