全文获取类型
收费全文 | 36200篇 |
免费 | 3460篇 |
国内免费 | 3408篇 |
出版年
2024年 | 134篇 |
2023年 | 912篇 |
2022年 | 1163篇 |
2021年 | 1633篇 |
2020年 | 1516篇 |
2019年 | 1816篇 |
2018年 | 1584篇 |
2017年 | 1514篇 |
2016年 | 1532篇 |
2015年 | 1662篇 |
2014年 | 2168篇 |
2013年 | 2976篇 |
2012年 | 1647篇 |
2011年 | 1975篇 |
2010年 | 1531篇 |
2009年 | 1897篇 |
2008年 | 1988篇 |
2007年 | 1837篇 |
2006年 | 1689篇 |
2005年 | 1456篇 |
2004年 | 1340篇 |
2003年 | 1081篇 |
2002年 | 955篇 |
2001年 | 821篇 |
2000年 | 737篇 |
1999年 | 655篇 |
1998年 | 567篇 |
1997年 | 500篇 |
1996年 | 414篇 |
1995年 | 374篇 |
1994年 | 414篇 |
1993年 | 326篇 |
1992年 | 309篇 |
1991年 | 256篇 |
1990年 | 228篇 |
1989年 | 180篇 |
1988年 | 165篇 |
1987年 | 143篇 |
1986年 | 148篇 |
1985年 | 104篇 |
1984年 | 113篇 |
1983年 | 99篇 |
1982年 | 129篇 |
1981年 | 84篇 |
1980年 | 83篇 |
1979年 | 55篇 |
1978年 | 48篇 |
1977年 | 22篇 |
1976年 | 23篇 |
1974年 | 21篇 |
排序方式: 共有10000条查询结果,搜索用时 265 毫秒
211.
Paton G., Thomas R. J. and Waller P. J. 1984. A prediction model for parasitic gastroenteritis in lambs. International Journal for Parasitology14: 439–445. The parasite Ostertagia circumcincta is a major cause of parasitic gastro-enteritis in lambs in temperate countries. A prediction model is described, based on a mathematical representation of the external and internal stages of the life-cycle.The model is used to predict the numbers of infective larvae on a permanent experimental paddock grazed by ewes and lambs in 1973 and 1974. The “moisture status” of the surface layer of the pasture was found to be of fundamental importance for the successful prediction of the development and survival of the pre-infective larval stages. For the years studied the contribution to the summer wave of infection by lamb derived larvae was particularly significant. 相似文献
212.
T.T. Dolan A.S. Young G.J. Losos I. McMilian Ch.E. Minder K. Soulsby 《International journal for parasitology》1984,14(1):89-95
Dolan T. T., Young A.S., Losos G.J., McMillan I., Minder Ch.E. and Soulsby K. 1984. Dose dependent responses of Theileria parva stabilate. International Journal for Parasitology14: 89–95. A tick derived stabilate of Theileria parva (Maguga) was titrated in a large group of Boran (Bos indicus) cattle of the same age, sex and origin. The infectivity data was analysed using the independent action model. The cattle were identified as heterogeneous in their response to infection with 75% showing one ID50 (0.0014) and 25% showing another (0.01). The disease responses of the cattle given different dose levels were compared for a variety of parameters. The results obtained showed these parameters to be dose dependent including the time to onset of piroplasm parasitaemia. The stabilate is of large volume and can be used for controlled challenge in immunity studies and for comparison of susceptibility between cattle of different breeds and from different epidemiological backgrounds. 相似文献
213.
214.
Summary A general method is presented for the formulation and numerical evaluation of mathematical models describing epithelial transport. The method is based on the principles of conservation of mass, and maintenance of electroneutrality within the cells and bathing solutions. It is therefore independent of the specific membrane transport mechanisms, and can be used to evaluate different models describing arbitrary transport processes (including passive, active and cotransport processes). Detailed numerical methods are presented that allow computation of steady-state and transient responses under open-circuit, current-clamp and voltage-clamp conditions, using a general-purpose laboratory minicomputer. To evaluate the utility of this approach, a specific model is presented that is consistent with the Koefoed-Johnson and Ussing hypothesis of sodium transport in tight epithelia (Acta Physiol. Scand.
42:298–308, 1958). This model considers passive transport of an arbitrary number of permeant solutes, active transport of sodium and potassium, and osmotically induced water transport across the apical and basolateral membranes. Results of the model are compared to published experimental measurements in rabbit urinary bladder epithelium. 相似文献
215.
自然界甘蓝茎点霉(Phoma lingam)的有性世代为斑点小球腔菌Leptosphaeriamaculans通常形成于甘蓝属植株的越冬残茎上。在实验室内其有性阶段不易培养或需要较长的时间,而且形成假囊壳的百分率也很低。为了在实验室内能短期诱发大量L.maculans的假囊壳,本实验研究了该菌假囊壳生成的条件,创造了双层培养方法。自澳大利亚采集到有该菌假囊壳的芸苔(Brassica napus)残基,分离得到子囊孢子单孢后代数十株,同时用保存的已知交配型665(+)和666(-)为材料,研究了(+)(-)配对结合的环境条件。实验结果表明16℃的温度,近紫外光照射(黑光灯)和V_(?)麦杆琼脂培养基上可以产生少量的假囊壳。但是,若配合以双层培养,则在培养4周后可产生大量成熟的假囊壳。实验还确定了该菌在性细胞形成、交配、子囊形成和成熟等阶段对环境因子都有不同的要求。双层培养对假囊壳的发育有重要作用。前期菌体营养生长与后期假囊壳的成熟紧密相关。 相似文献
216.
The conversion of aphid prey tissue (Acyrthosiphon pisum Harris) into predator biomass (immature life stages ofPropylaea 14-punctata L. andCoccinella 7-punctata L.) is calculated by plotting weight gain against assimilation (i.e. consumption minus egestion). This concept is added to
the metabolic pool model byGutierrez
et al. (1981) that enables the simulation of growth and development of a predator on a physiological basis. Physiological time is
expressed in daydegrees above lower development thresholds for both species. Visual examination of observed and calculated
values showed that the model satisfactorily describes the growth patterns of the above predators.
相似文献
217.
I. Lavorel 《Photosynthesis research》1986,9(1-2):273-283
The purpose of this note is to illustrate the feasibility of simulating kinetic systems, such as commonly encountered in photosynthesis research, using the Monte Carlo (MC) method. In this approach, chemical events are considered at the molecular level where they occur randomly and the macroscopic kinetic evolution results from averaging a large number of such events. Their repeated simulation is easily accomplished using digital computing. It is shown that the MC approach is well suited to the capabilities and resources of modern microcomputers. A software package is briefly described and discussed, allowing a simple programming of any kinetic model system and its resolution. The execution is reasonably fast and accurate; it is not subject to such instabilities as found with the conventional analytical approach.Abbreviations MC
Monte Carlo
- RN
random number
- PSU
photosynthetic unit
Dedicated to Prof. L.N.M. Duysens on the occasion of his retirement. 相似文献
218.
Subhash Padhye Takeshi Kambara David N. Hendrickson Govindjee 《Photosynthesis research》1986,9(1-2):103-112
The recent model of Kambara and Govindjee for water oxidation [Kambara T. and Govindjee (1985) Proc. Natl. Acad. Sci. U.S.A., 82:6119–6123] has been extended in this paper by examining all the data in order to identify the most likely candidate for the redox-active ligand (RAL), suggested to operate between the water oxidizing complex (WOC) and Z, the electron donor to the reaction center P680. We have concluded that a very suitable candidate for RAL is the imidazole moiety of a histidine residue. The electrochemical data available on imidazole derivatives play heavily in this identification of RAL. Thus, we suggest that histidine might play the role of an electron mediator between the WOC and Z. A model of S-states in terms of their plausible chemical identity is presented here.Abbreviations J
electronic spin of ion
- P680
reaction center chlorophyll
- RAL
Redox active ligand
- Sn
state of the oxygen-evolving system
- WOC
water oxidation complex
- Z
electron donor to P680
Dedicated to Prof. L.N.M. Duysens on the occasion of his retirement 相似文献
219.
Streptococcus mutans Ingbritt was grown in glucose-excess continuous culture to repress the glucose phosphoenolpyruvate phosphotransferase system (PTS) and allow investigation of the alternative glucose process using the non-PTS substrate, (3H) 6-deoxyglucose. After correcting for non-specific adsorption to inactivated cells, the radiolabelled glucose analogue was found to be concentrated approximately 4.3-fold intracellularly by bacteria incubated in 100 mM Tris-citrate buffer, pH 7.0. Mercaptoethanol or KCl enhanced 6-deoxyglucose uptake, enabling it to be concentrated internally by at least 8-fold, but NaCl was inhibitory to its transport. Initial uptake was antagonised by glucose but not 2-deoxyglucose. Evidence that 6-deoxyglucose transport was driven by protonmotive force (p) was obtained by inhibiting its uptake with the protonophores, 2,4-dinitrophenol, carbonylcyanide m-chlorophenylhydrazine, gramicidin and nigericin, and the electrical potential difference () dissipator, KSCN. The membrane ATPase inhibitor, N,N1-dicyclohexyl carbodiimide, also reduced 6-deoxyglucose uptake as did 100 mM lactate. In combination, these two inhibitors completely abolished 6-deoxyglucose transport. This suggests that the driving force for 6-deoxyglucose uptake is electrogenic, involving both the transmembrane pH gradient (pH) and . ATP hydrolysis, catalysed by the ATPase, and lactate excretion might be important contributors to pH.Abbreviations DNP
2,4-dinitrophenol
- CCCP
carbonylcyanide m-chlorophenylhydrazone
- DCCD
N,N1-dicyclohyxyl carbodiimide
- p
protonmotive force
- pH
transmembrane pH gradient
-
transmembrane electrical potential difference 相似文献
220.
Anatoli I. Yashin Kenneth G. Manton Eric Stallard 《Journal of mathematical biology》1986,24(2):119-140
Analyses of human mortality data classified according to cause of death frequently are based on competing risk theory. In particular, the times to death for different causes often are assumed to be independent. In this paper, a competing risk model with a weaker assumption of conditional independence of the times to death, given an assumed stochastic covariate process, is developed and applied to cause specific mortality data from the Framingham Heart Study. The results generated under this conditional independence model are compared with analogous results under the standard marginal independence model. Under the assumption that this conditional independence model is valid, the comparison suggests that the standard model overestimates by 4% the effect on life expectancy at age 30 due to the hypothetical elimination of cancer and by 7% the effect for cardiovascular/cerebrovascular disease. By age 80 the overestimates were 11% for cancer and 16% for heart disease. These results suggest the importance of avoiding the marginal independence assumption when appropriate data are available — especially when focusing on mortality at advanced ages. 相似文献