iASPP protects the heart from ischemia injury by inhibiting p53 expression and cardiomyocyte apoptosis

Currently, there remains a great need to elucidate the molecular mechanism of acute myocardial infarction in order to facilitate the development of novel therapy. Inhibitor of apoptosis-stimulating protein of p53 (iASPP) is a member of the ASPP family proteins and an evolutionarily preserved inhibitor of p53 that is involved in many cellular processes, including apoptosis of cancer cells. The purpose of this study was to investigate the possible role of iASPP in acute myocardial infarction. The protein level of iASPP was markedly reduced in the ischemic hearts in vivo and hydrogen peroxide-exposed cardiomyocytes in vitro. Overexpression of iASPP reduced the infarct size and cardiomyocyte apoptosis of mice subjected to 24 h of coronary artery ligation. Echocardiography showed that cardiac function was improved as indicated by the increase in ejection fraction and fractional shortening. In contrast, knockdown of iASPP exacerbated cardiac injury as manifested by impaired cardiac function, increased infarct size, and apoptosis rate. Mechanistically, overexpression of iASPP inhibited, while knockdown of iASPP increased the expressions of p53 and Bax, the key regulators of apoptosis. Taken together, our results suggested that iASPP is an important regulator of cardiomyocyte apoptosis, which represents a potential target in the therapy of myocardial infarction.     

靛蓝对葡聚糖硫酸钠诱导的溃疡性结肠炎小鼠肠道菌群作用研究

目的 研究靛蓝对葡聚糖硫酸钠（DSS）诱导的溃疡性结肠炎（UC）模型小鼠的干预作用,并分析对小鼠肠道菌群的影响。方法实验小鼠分为对照组、模型组、柳氮磺胺吡啶组（125 mg/kg）和靛蓝组（50mg/kg),每组小鼠各9只。观察给药后小鼠体征并进行疾病活动指数（DAI）评分,通过苏木素―伊红（HE）染色观察小鼠结肠组织切片形态变化,ELISA法检测小鼠血清中IL-6、TNF-α、IL-1β、IL-8和IL-10水平;针对16S rRNA基因V4-V5区进行高通量测序,分析小鼠肠道内容物的菌群变化。结果 与模型组相比,靛蓝组小鼠DAI评分降低,病理切片结果显示靛蓝可改善UC小鼠结肠黏膜损伤,减少炎性细胞浸润,血清中促炎因子IL-6、IL-8、IL-1β和TNF-α水平显著降低（t=4.377 0、5.374 0、12.140 0、5.508 0,P=0.011 9、0.005 8、0.000 3、0.005 3）,抑炎因子IL-10水平显著升高（t=3.716 0,P=0.020 5）。16S rRNA基因测序结果显示,模型组小鼠肠道菌群多样性降低,靛蓝组小鼠肠道菌群多样性升高。结论 给...     

人乳头瘤病毒感染对健康女性阴道菌群的影响

目的 筛查健康女性人乳头瘤病毒（human papillomavirus,HPV）感染状况,探究HPV感染对阴道微生态的影响。方法 入组54例健康女性,采用实时荧光定量PCR法测定HPV感染亚型,16S rRNA高通量测序技术分析HPV感染对阴道菌群的物种多样性及结构组成的影响。结果 HPV筛查后发现,12.96%的女性未感染HPV,而87.04%的女性有HPV感染,其中42.55%为HPV 16高危型感染,57.45%为HPV多种亚型混合感染。HPV感染后,健康女性阴道菌群的物种多样性有增加的趋势;HPV混合感染会降低乳杆菌的相对丰度,增加链球菌和普雷沃菌的相对丰度。结论 健康女性HPV高危型感染率较高,HPV感染会加剧阴道微生态失衡,需及时监测和采取有效的干预措施。     

基于16S rRNA高通量测序技术分析福州地区慢传输型便秘患者肠道菌群的变化

目的 运用16S rRNA高通量测序技术分析福州地区慢传输型便秘(STC)患者肠道菌群变化的特征。方法 纳入60例STC患者和健康志愿者20例,留取新鲜粪便样本,冰块运送至实验室并存放于-80度冰箱,应用16S rRNA高通量测序技术,分析各组肠道菌群的生物多样性与结构组成。结果 测序后共得到1 702 004 524条有效序列,样本序列平均有效长度为414.1 bp,总样本平均序列条数为2 127 505.7条。与对照组比较,STC患者肠道菌群丰度指数Ace指数、Chao指数和肠道菌群多样性指数Shannon指数下降(P<0.05),肠道菌群多样性指数Simpson指数显著升高(P<0.05)。在门水平上,STC组以拟杆菌门、放线菌门为主,正常对照组以厚壁菌门为主。在属水平上,STC组以拟杆菌属(Bacteroides)、肠杆菌科(Enterobacteriaceae)、丹毒杆菌属(Erysipelatoclostridium)、巨单胞菌属(Megamonas)为主,正常对照组以乳酸杆菌属(Lactobacillus)、双歧杆菌属(Bifidobacterium)、布劳特...     

16S rDNA实时荧光定量PCR检测大蒜提取物对小鼠肠道菌群的调节作用

目的 探究蒜氨酸、大蒜辣素、大蒜粉对小鼠肠道菌群的调节作用。方法 将SPF级C57BL/6J小鼠分为空白对照组、蒜氨酸组、大蒜辣素组和大蒜粉组,每组10只。空白对照组小鼠灌服蒸馏水30 d,蒜氨酸组、大蒜辣素组、大蒜粉组小鼠灌服不同浓度的受试物溶液30 d,采用16S rDNA实时荧光定量PCR检测实验前后小鼠粪便样本中乳杆菌、双歧杆菌、肠杆菌、肠球菌、产气荚膜梭菌水平变化。结果 给药30 d后,与空白对照组相比、实验前后自身对比,蒜氨酸组、大蒜辣素组、大蒜粉组小鼠的肠杆菌、肠球菌水平无明显变化;乳杆菌、双歧杆菌水平显著增加;产气荚膜梭菌水平显著降低。结论 蒜氨酸、大蒜辣素、大蒜粉对肠道菌群具有有益的调节作用,是一种良好的天然有益于肠道菌群的食品。     

隔离降雨对马尾松针叶非结构性碳水化合物和碳、氮、磷的影响

植物叶片中的非结构性碳水化合物(NSC)和碳(C)、氮(N)、磷(P)含量可反映植物和生态系统对水分亏缺环境的响应及适应程度。以福建省长汀县25a马尾松(Pinus massoniana)为对象,原位观测分析了3年持续100%隔离降雨对成年马尾松针叶NSC与C、N、P的影响。结果表明：(1)持续隔离降雨导致马尾松针叶NSC含量先显著增加后减少,最终导致针叶NSC含量季节变化消失;且针叶可溶性糖含量、可溶性糖/淀粉比值在后期显著增加;(2)持续隔离降雨使马尾松针叶N含量、P含量、N∶P均表现为前期与对照组差异不显著,后期显著高于对照组;(3)马尾松针叶NSC含量、可溶性糖含量、P含量、N∶P与土壤水分含量呈显著或极显著相关,且均与P含量显著或极显著相关,与N素无相关性。以上研究结果表明,随着土壤水分含量的持续减少马尾松可通过调整针叶中NSC含量的积累及分配和提高针叶N、P含量来适应缺水环境,P含量的增加对NSC含量波动及可溶性糖和淀粉的相互转化起促进作用。     

猫基底前脑胆碱能皮质投射神经元区的P物质样免疫反应神经元

应用免疫组织化学和邻位切片法,研究了猫基底前脑胆碱能皮质投射神经元区的Ｐ物质样免疫反应神经元的分布特征,及其与胆碱乙酰化酶样免疫反应神经元的分布关系,从内侧隔核至Ｍｅｙｎｅｒｔ基底核,２种神经元分布范围相近,且形态,大小相似。但在邻位切片,未见分别呈此二免疫反应的同一神经元的对应剖面。Ｐ物质样免疫反应神经元在内侧隔核和斜角带核数量较多,但在基底核明显减少,在Ｃｈ４间质部及腹侧苍白球连合下部仅为偶见     

Partial characterization of the N－linked oligosaccharide structures on Pselectin glycoprotein ligand－1（PSGL－1）

PSGL-1, a specific ligand for P-, E- and L-selectin, was isolated from in vivo [3H]-glucosamine labeled HL-60 cells by a combination of wheat germ agglutinin-agarose and P- or E-selectin-agarose chromatography. N-linked oligosaccharides were released from the purified, denatured ligand molecule by peptide: N-glycosidase F treatment and, following separation by Sephacryl S-200 chromatography, partially characterized using lectin, ion-exchange and size-exclusion chromatography in combination with glycosidase digestions. The data obtained suggest that the N-glycans on PSGL-1 are predominantly core-fucosylated, multiantennary complex type structures with extended, poly-N-acetyllactosamine containing outer chains. A portion of the outer chains appears to be substituted with fucose indicating that the N-glycans, in addition to the O-glycans on PSGL-1, may be involved in selectin binding.     

P16蛋白和生精细胞凋亡对热压和11酸睾酮诱导的恒河猴无精子症和少精子症中的作用

探讨了P16蛋白和生精细胞凋亡在热压和11酸睾酮诱导恒河猴无精子症和少精子症中作用间的关系。3′末端标记分析（TUNEL）结果显示热应激和超生理剂量睾酮能够诱导生精细胞出现凋亡信号,它分别于处理后第5天和第30天达到最强,免疫组化结果显示,热压或TU主要诱导精原细胞和其它生精细胞以及Sertoli细胞P16的表达。P16蛋白的表达在生精细胞凋亡晚期,即隐睾手术第10天或注射TU第60天后迅速升高并维持在热压或11酸睾酮诱导的早期精母细胞和精子细胞的凋亡和在晚期对精原细胞有丝分裂的抑制,二者共同作用导致热压或TU诱导的恒河猴无精子症和少精子症。