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41.
Helminth community composition and richness were studied in brown trout, Salmo trutta , in 10 reservoirs of broadly similar age and characteristics situated close to each other in a well-defined region of south-west England. Communities were compared using cluster and ordination analyses, and possible correlations between helminth richness and a number of environmental variables were investigated. The hypothesis that the helminth communities should show high degrees of similarity and that large differences between reservoirs and a high degree of clustering would be unrecognizable was refuted. Levels of community similarity were low and comparable to those determined for helminths in salmonids in natural lakes. Trout in some reservoirs exhibited very distinctive helminth faunas and clustering of reservoirs was apparent. No single factor, including reservoir size and presence of piscivorous birds, had a predominant influence on community richness or composition but rather a multiplicity of local factors was believed to influence these parameters. The results indicate that local factors promoting distinctiveness have a greater influence on the composition and richness of fish helminth communities in lakes and reservoirs than do regional factors promoting similarity. 相似文献
42.
R. C. Hart 《African Journal of Aquatic Science》2013,38(1-2):20-41
Summary An overview of a three year study of abundance, community structure and succession of zooplankton and phytoplankton in relation to physical limnological conditions in Lakes Midmar and Albert Falls is presented, along with findings on zooplankton from a nine month study of Lake Nagle. Physical stability increased between Midmar (mean summer N2 = 6.55 × 104 s2) and Albert Falls (N2 = 8.70 × 104 s2), in line with elevations in water temperature. Phytoplankton richness and diversity was similar in these reservoirs, although zooplankton species richness increased downstream. Abundance levels of both phyto- and zoo-plankton were broadly comparable in these two reservoirs. In Midmar and Albert Falls, overall mean (± SD) chlorophyll levels were 3.60 ± 1.54 and 3.41 ± 1.41 μg 11, with corresponding overall average zooplankton standing stocks of 0.85 and 0.76 g m2 dry mass, dropping to 0.54 g m2 in Nagle (for which spring to midsummer data are missing). Ruderal and colonist phytoplankters were persistently dominant both in Midmar and Albert Falls, in keeping with the continuous (if incomplete) mixing patterns evident in these reservoirs. Few stress tolerant algae occurred. The summer ruderal assemblage was unexpectedly dominated by diatoms in the physically more stable conditions of Albert Falls, but not Midmar. Mean zooplankton grazer-induced instantaneous mortality rates for planktonic algae for the whole study were estimated (by regression predictions) as 0.17 d1 in both lakes. Phytoplankton was not studied in Nagle. Changes in zooplankton community structure between Midmar and Nagle largely involved progressive increases in the contribution of smaller-bodied cladocerans (comprising both sequentially smaller species of Daphnia (D. pulex, D. longispina, D. laevis), and additional genera such as Diaphanosoma, Ceriodapnia, Moina and Bosmina). As with a parallel progressive switch between Metadiaptomus meridianus and Tropodiaptomus spectabilis over this series of reservoirs, (and the temporal separation of “co-existing” populations in Albert Falls), which has been shown experimentally to be strongly temperature-linked, changes in absolute temperature are implicated as a primary causal factor in the shifts in cladoceran species composition. Temporal occurrences of these species also indicate the primacy of temperature. 相似文献
43.
Pascale Jolivet Francisca Acevedo Céline Boulard Sabine d'Andréa Jean‐Denis Faure Ajay Kohli Nathalie Nesi Benoit Valot Thierry Chardot 《Proteomics》2013,13(12-13):1836-1849
Oleaginous seeds store lipids in specialized structures called oil bodies (OBs). These organelles consist of a core of neutral lipids bound by proteins embedded in a phospholipid monolayer. OB proteins are well conserved in plants and have long been grouped into only two categories: structural proteins or enzymes. Recent work, however, which identified other classes of proteins associated with OBs, clearly shows that this classification is obsolete. Proteomics‐mediated OB protein identification is facilitated in plants for which the genome is sequenced and annotated. However, it is not clear whether this knowledge can be dependably transposed to less well‐characterized plants, including the well‐established commercial sources of seed oil as well as the many others being proposed as novel sources for biodiesel, especially in Africa and Asia. Toward an update of the current data available on OB proteins this review discusses (i) the specific difficulties for proteomic studies of organelles; (ii) a 2012 census of the proteins found in seed OBs from various crops; (iii) the oleosin composition of OBs and their role in organelle stability; (iv) PTM of OB proteins as an emerging field of investigation; and finally we describe the emerging model of the OB proteome from oilseed crops. 相似文献
44.
HIV entry into the CNS is an early event after peripheral infection, resulting in neurologic dysfunction in a significant number of individuals despite successful anti‐retroviral therapy. The mechanisms by which HIV mediates CNS dysfunction are not well understood. Our group recently demonstrated that HIV infection of astrocytes results in survival of HIV infected cells and apoptosis of surrounding uninfected astrocytes by the transmission of toxic intracellular signals through gap junctions. In the current report, we characterize the intracellular signaling responsible for this bystander apoptosis. Here, we demonstrate that HIV infection of astrocytes results in release of cytochrome C from the mitochondria into the cytoplasm, and dysregulation of inositol trisphosphate/intracellular calcium that leads to toxicity to neighboring uninfected astrocytes. Blocking these dysregulated pathways results in protection from bystander apoptosis. These secondary messengers that are toxic in uninfected cells are not toxic in HIV infected cells, suggesting that HIV protects these cells from apoptosis. Thus, our data provide novel mechanisms of HIV mediated toxicity and generation of HIV reservoirs. Our findings provide new potential therapeutic targets to reduce the CNS damage resulting from HIV infection and to eradicate the generation of viral reservoirs.
45.
Polarizing near-field scanning optical microscopy (NSOM) was applied for visualization of lipid coagel structures. The technique
ensures obtaining polarization contrast images at micro- and nanoscale resolution. Comparison to the polarizing light microscopy
images revealed that the same fractal structural organization persists also at submicron scale, at the level of primary ordered
structures creation. Many long birefringent needle-shaped primary crystallites were imaged in the corn oil:monoglyceride samples,
and lower amount of smaller oval-shaped primary crystallites—in the olive oil:monoglyceride samples. Unlike atomic force microscopy,
polarizing NSOM brought direct evidence on the physical state of specific features. Compared to the polarizing light microscopy,
polarizing NSOM provided additional information on the structural organization of oil–monoglyceride coagels at the micro-
and submicron scale. 相似文献
46.
Field observations on chlorophyll content and hydrological parameters such as temperature, dissolved oxygen and conductivity profiles, Secchi disk transparency and water color were carried out at 5 reservoirs of the Mid and Lower Volga River during August 1990 and 1991. Principal Component Analysis distinguished four classes from 46 sampling points based on the hydrological regimen and phytoplankton development. 相似文献
47.
Characterization of bacterial strains able to grow on high molecular mass residues from crude oil processing 总被引:9,自引:0,他引:9
Yuste L Corbella ME Turiégano MJ Karlson U Puyet A Rojo F 《FEMS microbiology ecology》2000,32(1):69-75
Oil residues containing high molecular mass hydrocarbons, rich in polyaromatic compounds, are frequent end-products of crude oil processing and are poorly biodegradable. Their disposal poses an environmental problem. Through batch-enrichments from contaminated soils we have isolated and characterized seven bacterial strains that can use a residue from crude oil processing as a source of carbon and energy. The residue was a complex mixture of high molecular mass compounds, including saturated, aromatic and polycyclic aromatic hydrocarbons (PAHs). Analysis of the metabolic profiles of the strains isolated showed that they could all metabolize long-chain-length alkanes efficiently, but not PAHs. Strains degrading naphthalene, a simple PAH, did exist in the soil inocula used, but could be isolated only when enrichments were performed using pure naphthalene as the sole carbon source. All strains tested emulsified the oil residue and their ability to produce surfactants was studied. 相似文献
48.
Isolation of thermotolerant,halotolerant, facultative biosurfactant-producing bacteria 总被引:1,自引:0,他引:1
Ghojavand H Vahabzadeh F Mehranian M Radmehr M Shahraki KhA Zolfagharian F Emadi MA Roayaei E 《Applied microbiology and biotechnology》2008,80(6):1073-1085
Several facultative bacterial strains tolerant to high temperature and salinity were isolated from the oil reservoir brines
of an Iranian oil field (Masjed-I Soleyman). Some of these isolates were able to grow up to 60°C and at high concentration
of NaCl (15% w/v). One of the isolates grew at 40°C, while it was able to grow at 15% w/v NaCl. Tolerances to NaCl levels decreased as the growth temperatures were increased. Surfactant production ability was detected
in some of these isolates. The use of biosurfactant is considered as an effective mechanism in microbial-enhanced oil recovery
processes detected in some of these isolates. The surfactant producers were able to grow at high temperatures and salinities
to about 55°C and 10% w/v, respectively. These isolates exhibited morphological and physiological characteristics of the Bacillus genus. The partial sequencing of the 16S ribosomal deoxyribonucleic acid gene of the selected isolates was assigned them
to Bacillus subtilis group. The biosurfactant produced by these isolates caused a substantial decrease in the surface tension of the culture media
to 26.7 mN/m. By the use of thin-layer chromatography technique, the presence of the three compounds was detected in the tested
biosurfactant. Infrared spectroscopy and 1H nuclear magnetic resonance analysis were used, and the partial structural characterization of the biosurfactant mixture
of the three compounds was found to be lipopeptidic in nature. The possibility of use of the selected bacterial strains reported,
in the present study, in different sectors of the petroleum industry has been addressed. 相似文献
49.
The castor-bean endosperm-the best-studied material of reserve lipid hydrolysis in seed germination-was previously shown to have an acid lipase and an alkaline lipase having reciprocal patterns of development during germination. We studied oil seeds from 7 species, namely castor bean (Ricinus communis L.), peanut (Arachis hypogaea L.), sunflower (Helianthus annus L.), cucumber (Cucumis sativus L.), cotton (Gossypisum hirsutum L.), corn (Zea mays. L.) and tomato (Lycopersicon esculentum Mill.). The storage tissues of all these oil seeds except castor bean contained only alkaline lipase activity which increased drastically during germination. The pattern of acid and alkaline lipases in castor bean does not seem to be common in other oil seeds. The alkaline lipase of peanut cotyledons was chosen for further study. On sucrose gradient centrifugation of cotyledon homogenate from 3-d-old seedlings, about 60% of the activity of the enzyme was found to be associated with the glyoxysomes, 15% with the mitochondria, and 25% with a membrane fraction at a density of 1.12 g cm-3. The glyoxysomal lipase was associated with the organelle membrane, and hydrolyzed only monoglyceride whereas the mitochondrial and membrane-fraction enzymes degraded mono-, di- and triglycerides equally well. Thus, although the lipase in the glyoxysomes had the highest activity, it had to cooperate with lipases in other cellular compartments for the complete hydrolysis of reserve triglycerides. 相似文献
50.