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91.
LRRC25 inhibits type I IFN signaling by targeting ISG15‐associated RIG‐I for autophagic degradation 下载免费PDF全文
The RIG‐I‐like receptors (RLRs) are critical for protection against RNA virus infection, and their activities must be stringently controlled to maintain immune homeostasis. Here, we report that leucine‐rich repeat containing protein 25 (LRRC25) is a key negative regulator of RLR‐mediated type I interferon (IFN) signaling. Upon RNA virus infection, LRRC25 specifically binds to ISG15‐associated RIG‐I to promote interaction between RIG‐I and the autophagic cargo receptor p62 and to mediate RIG‐I degradation via selective autophagy. Depletion of either LRRC25 or ISG15 abrogates RIG‐I‐p62 interaction as well as the autophagic degradation of RIG‐I. Collectively, our findings identify a previously unrecognized role of LRRC25 in type I IFN signaling activation by which LRRC25 acts as a secondary receptor to assist RIG‐I delivery to autophagosomes for degradation in a p62‐dependent manner. 相似文献
92.
Moran D.G. Yoshida S. Fujiyama K. Seki T. Yoshida T. 《World journal of microbiology & biotechnology》1997,13(3):265-267
A truncated Man9-mannosidase gene and a full-length -1,4-galactosyltransferase gene were isolated from human kidney and placenta cDNAs, respectively. Both genes were cloned in plasmid pMAL-c2 to produce fusions with maltose-binding protein. Fusion products were purified by affinity chromatography. Purified enzymes were assayed using pyridyl-amino labelled oligosaccharides as substrates and analysed by HPLC. 相似文献
93.
Umul Kulthum Ahmed N. Claire Maller Asif J. Iqbal Lamyaa Al-Riyami William Harnett John G. Raynes 《The Journal of biological chemistry》2016,291(22):11939-11953
Parasitic nematodes manufacture various carbohydrate-linked phosphorylcholine (PCh)-containing molecules, including ES-62, a protein with an N-linked glycan terminally substituted with PCh. The PCh component is biologically important because it is required for immunomodulatory effects. We showed that most ES-62 was bound to a single protein, C-reactive protein (CRP), in normal human serum, displaying a calcium-dependent, high-avidity interaction and ability to form large complexes. Unexpectedly, CRP binding to ES-62 failed to efficiently activate complement as far as the C3 convertase stage in comparison with PCh-BSA and PCh-containing Streptococcus pneumoniae cell wall polysaccharide. C1q capture assays demonstrated an ES-62-CRP-C1q interaction in serum. The three ligands all activated C1 and generated C4b to similar extents. However, a C2a active site was not generated following ES-62 binding to CRP, demonstrating that C2 cleavage was far less efficient for ES-62-containing complexes. We proposed that failure of C2 cleavage was due to the flexible nature of carbohydrate-bound PCh and that reduced proximity of the C1 complex was the reason that C2 was poorly cleaved. This was confirmed using synthetic analogues that were similar to ES-62 only in respect of having a flexible PCh. Furthermore, ES-62 was shown to deplete early complement components, such as the rate-limiting C4, following CRP interaction and thereby inhibit classical pathway activation. Thus, flexible PCh-glycan represents a novel mechanism for subversion of complement activation. These data illustrate the importance of the rate-limiting C4/C2 stage of complement activation and reveal a new addition to the repertoire of ES-62 immunomodulatory mechanisms with possible therapeutic applications. 相似文献
94.
95.
Alice Goode Kevin Butler Jed Long James Cavey Daniel Scott Barry Shaw 《Autophagy》2016,12(7):1094-1104
Growing evidence implicates impairment of autophagy as a candidate pathogenic mechanism in the spectrum of neurodegenerative disorders which includes amyotrophic lateral sclerosis and frontotemporal lobar degeneration (ALS-FTLD). SQSTM1, which encodes the autophagy receptor SQSTM1/p62, is genetically associated with ALS-FTLD, although to date autophagy-relevant functional defects in disease-associated variants have not been described. A key protein-protein interaction in autophagy is the recognition of a lipid-anchored form of LC3 (LC3-II) within the phagophore membrane by SQSTM1, mediated through its LC3-interacting region (LIR), and notably some ALS-FTLD mutations map to this region. Here we show that although representing a conservative substitution and predicted to be benign, the ALS-associated L341V mutation of SQSTM1 is defective in recognition of LC3B. We place our observations on a firm quantitative footing by showing the L341V-mutant LIR is associated with a ~3-fold reduction in LC3B binding affinity and using protein NMR we rationalize the structural basis for the effect. This functional deficit is realized in motor neuron-like cells, with the L341V mutant EGFP-mCherry-SQSTM1 less readily incorporated into acidic autophagic vesicles than the wild type. Our data supports a model in which the L341V mutation limits the critical step of SQSTM1 recruitment to the phagophore. The oligomeric nature of SQSTM1, which presents multiple LIRs to template growth of the phagophore, potentially gives rise to avidity effects which amplify the relatively modest impact of any single mutation on LC3B binding. Over the lifetime of a neuron, impaired autophagy could expose a vulnerability, which ultimately tips the balance from cell survival toward cell death. 相似文献
96.
97.
Jeong Su Park Sue Young Oh Da Hyun Lee Yu Seol Lee Su Haeng Sung Hye Won Ji 《Free radical research》2016,50(12):1408-1421
Endoplasmic reticulum (ER) stress is triggered by various cellular stresses that disturb protein folding or calcium homeostasis in the ER. To cope with these stresses, ER stress activates the unfolded protein response (UPR) pathway, but unresolved ER stress induces reactive oxygen species (ROS) accumulation leading to apoptotic cell death. However, the mechanisms that underlie protection from ER stress-induced cell death are not clearly defined. The nuclear factor erythroid 2-related factor 2 (Nrf2)-Kelch-like ECH-associated protein 1 (Keap1) pathway plays a crucial role in the protection of cells against ROS-mediated oxidative damage. Keap1 acts as a negative regulator of Nrf2 activation. In this study, we investigated the role of the Nrf2-Keap1 pathway in protection from ER stress-induced cell death using tunicamycin (TM) as an ER stress inducer. We found that Nrf2 is an essential protein for the prevention from TM-induced apoptotic cell death and its activation is driven by autophagic Keap1 degradation. Furthermore, ablation of p62, an adapter protein in the autophagy process, attenuates the Keap1 degradation and Nrf2 activation that was induced by TM treatment, and thereby increases susceptibility to apoptotic cell death. Conversely, reinforcement of p62 alleviated TM-induced cell death in p62-deficient cells. Taken together, these results demonstrate that p62 plays an important role in protecting cells from TM-induced cell death through Nrf2 activation. 相似文献
98.
J Martinsen M J Irwin P S Ho B M Hoffman I M Klotz 《Biochemical and biophysical research communications》1983,112(3):954-956
Electron paramagnetic resonance spectra of azidosemimethemerythrin (from Phascolopsis gouldii) have been integrated to find the total number of spins per monomer unit. The value observed, 1.0 +/- 0.1 spins per Fe2 pair, confirms the assignment of a hybrid oxidation state, FeIIFeIII, to each site. 相似文献
99.
Cells of Paracoccus denitrificans grown autotrophically with H2 as energy source contained a branched respiratory chain. The presence of two terminal oxidases was indicated by two cyanide sensitive sites (K
i
=10-5 M and K
i
=10-3 M). While oxidation of NADH and succinate apparently proceeded via both electron pathways as shown by the inhibition of respiration with cyanide and Antimycin A, oxidation of H2 involved only the terminal oxidase which was less sensitive to KCN. Oxidation of H2 was not inhibited by rotenone, and sensitive to only relatively high concentrations of Antimycin A (50 nmol/mg).Under our growth conditions, autotrophic cells contained only very small amounts of cytochrome a +a
3
. A cytochrome b was able to bind CO (with a peak at 418 nm and a trough at 434 nm in the reduced plus CO minus reduced difference spectrum). This cytochrome b had the spectral characteristics of cytochrome o and could be the alternate oxidase. The respiratory chain contained two b cytochromes (b
556 and b
562 at 77°K); under steady state conditions only b
556 was significantly reduced by NADH and succinate while both b
556 and b
562 were reduced by H2.Measurement of respiration-driven proton translocation by spheroplasts showed that the oxidation of H2 by O2 was associated with a vectorial ejection of H+ (in the outward direction) with aH+/O value of 6 to 7.A similar result was obtained with succinate. Oxidation of endogenous substrates gave H+/O values corresponding to a H+/site ratio of 3 with 3 sites functioning in absence of inhibitors, two sites in the presence of rotenone and one site in the presence of antimycin. The H+/O values indicated that two energy transducing sites were involved in the oxidation of H2 by O2.Measurement of ATP synthesis in membrane vesicles confirmed that phosphorylation was coupled to H2 oxidation. However, such determinations which necessitated the use of inverted vesicles, gave P/O values too low to allow any conclusions to be made on the number of coupling sites. 相似文献
100.