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101.
Atmospheric CO2 concentration has increased by 25% over the preindustrial level. A parallel increase in C concentration and decreases in N
concentration and δ13C of plants grown throughout this century have been observed in plant specimens stored in herbaria. We tested our previous
results in a study of 12 more species collected in the western Mediterranean throughout this century (1920–1930, 1945–1955,
and 1985–1990) and tree rings of Quercus pubescens from the same area. These changes were accompanied by apparent increases in condensed tannin concentration. A decreasing trend
in δ15N both in herbarium material and tree rings was also found, indicating that ecosystems might cope with higher plant N demand
by decreasing N losses and increasing N fixation and mineralization. These results may contribute to a better understanding
of the effects of global change on carbon and nitrogen cycling.
Received: 12 November 1995 / Accepted: 17 May 1996 相似文献
102.
Nitrogen losses from perennial grass species 总被引:1,自引:0,他引:1
Nitrogen losses from plants may occur through a variety of pathways, but so far, most studies have only quantified losses of nutrients by above-ground litter production. We used 15N pulse labelling to quantify total nitrogen losses from above- and below-ground plant parts. Using this method we were able to include also pathways other than above-ground litter production. To test the hypothesis that species from nutrient-poor habitats lose less nitrogen than species from more fertile soils, six perennial grasses from habitats with a wide range of nutrient availability were investigated: Lolium perenne, Arrhenatherum elatius, Anthoxanthum odoratum, Festuca rubra, F. ovina and Molinia caerulea. The results of an experiment carried out in pots in a green-house at two fertility levels show that statistically significant losses occur through pathways other than above-ground litter production. In the low fertility treatment, most (70%) losses from L. perenne occurred by litter production, but in Ar. elatius, F. rubra, F. ovina and M. caerulea, more than 50% of labelled N losses took place by root turn-over, leaching or exudation from roots. When nutrient supply increased, the 15N losses in above-ground dead material increased in all species and in Ar. elatius, A. odoratum and F. rubra the 15N losses via other pathways decreased. Ranked according to decreasing turnover coefficient the sequence of species was: L. perenne, A. odoratum, F. rubra, F. ovina, Ar. elatius, M. caerulea. These results suggest that species adapted to sites with low availability of nutrients lose less nitrogen (including above- and below-ground losses) than species adapted to more fertile soils. 相似文献
103.
Effects of elevated CO2 and increased nitrogen deposition on photosynthesis and growth of understory plants in spruce model ecosystems 总被引:1,自引:0,他引:1
We studied the effects of atmospheric CO2 enrichment (280, 420 and 560 l CO2 l–1) and increased N deposition (0,30 and 90 kg ha–1 year–1) on the spruce-forest understory species Oxalis acetosella, Homogyne alpina and Rubus hirtus. Clones of these species formed the ground cover in nine 0.7 m2 model ecosystems with 5-year-old Picea abies trees (leaf area index of approx 2.2). Communities grew on natural forest soil in a simulated montane climate. Independently of N deposition, the rate of light-saturated net photosynthesis of leaves grown and measured at 420 l CO2 l–1 was higher in Oxalis and in Homogyne, but was not significantly different in Rubus compared to leaves grown and measured at the pre-industrial CO2 concentration of 280 l l–1. Remarkably, further CO2 enrichment to 560 l l–1 caused no additional increase of CO2 uptake. With increasing CO2 supply concentrations of non-structural carbohydrates in leaves increased and N concentrations decreased in all species, whereas N deposition had no significant effect on these traits. Above-ground biomass and leaf area production were not significantly affected by elevated CO2 in the more vigorously growing species O. acetosella and R. hirtus, but the slow growing H. alpina produced almost twice as much biomass and 50% more leaf area per plant under 420 l CO2 l–1 compared to 280 l l–1 (again no further stimulation at 560 l l–1). In contrast, increased N addition stimulated growth in Oxalis and Rubus but had no effect on Homogyne. In Oxalis (only) biomass per plant was positively correlated with microhabitat quantum flux density at low CO2, but not at high CO2 indicating carbon saturation. On the other hand, the less shade-tolerant Homogyne profited from CO2 enrichment at all understory light levels facilitating its spread into more shady micro-habitats under elevated CO2. These species-specific responses to CO2 and N deposition will affect community structure. The non-linear responses to elevated CO2 of several of the traits studied here suggest that the largest responses to rising atmospheric CO2 are under way now or have already occurred and possible future responses to further increases in CO2 concentration are likely to be much smaller in these understory species. 相似文献
104.
Steve P. McGrath Amar M. Chaudri Ken E. Giller 《Journal of industrial microbiology & biotechnology》1995,14(2):94-104
Summary This paper reviews the evidence for impacts of metals on the growth of selected plants and on the effects of metals on soil microbial activity and soil fertility in the long-term. Less is known about adverse long-term effects of metals on soil microorganisms than on crop yields and metal uptake. This is not surprising, since the effects of metals added to soils in sewage sludge are difficult to assess, and few long-term experiments exist. Controlled field experiments with sewage sludges exist in the UK, Sweden, Germany and the USA and the data presented here are from these long-term field experiments only. Microbial activity and populations of cyanobacteria,Rhizobium leguminosarum bv.trifolii, mycorrhizae and the total microbial biomass have been adversely affected by metal concentrations which, in some cases, are below the European Community's maximum allowable concentration limits for metals in sludge-treated soils. For example, N2-fixation by free living heterotrophic bacteria was found to be inhibited at soil metal concentrations of (mg kg–1): 127 Zn, 37 Cu, 21 Ni, 3.4 Cd, 52 Cr and 71 Pb. N2-fixation by free-living cyanobacteria was reduced by 50% at metal concentrations of (mg kg–1): 114 Zn, 33 Cu, 17 Ni, 2.9 Cd, 80 Cr and 40 Pb.Rhizobium leguminosarum bv.trifolii numbers decreased by several orders of magnitude at soil metal concentrations of (mg kg–1): 130–200 Zn, 27–48 Cu, 11–15 Ni, and 0.8–1.0 Cd. Soil texture and pH were found to influence the concentrations at which toxicity occurred to both microorganisms and plants. Higher pH, and increased contents of clay and organic carbon reduced metal toxicity considerably. The evidence suggests that adverse effects on soil microbial parameters were generally found at surpringly modest concentrations of metals in soils. It is concluded that prevention of adverse effects on soil microbial processes and ultimately soil fertility, should be a factor which influences soil protection legislation. 相似文献
105.
羊草草地枯枝落叶中N,P,K变化动态 总被引:7,自引:2,他引:5
羊草草地枯枝落叶中N、P、K变化动态郭继勋,祝廷成(东北师范大学国家草地生态工程实验室,长春130024)DynamicsofN,PandKinlittersonAneurolopidiumchinensegrassland.¥GuoJixunand... 相似文献
106.
Jan Michiels Inge D'hooghe Christel Verreth Heidi Pelemans Jos Vanderleyden 《Archives of microbiology》1994,161(5):404-408
We report the isolation, mutational analysis and the nucleotide sequence of the Rhizobium leguminosarum bv. phaseoli nifA gene. Comparison of the deduced amino acid sequence with other NifA sequences indicated the presence of the conserved central activator and the C-terminal DNA-binding domains. Nodules elicited by a R. leguminosarum bv. phaseoli nifA mutant were symbiotically ineffective. The expression of a nifA-gusA fusion was shown to be independent on the oxygen status of the cell. We cloned the three nifH copies of R. leguminosarum bv. phaseoli and determined the nucleotide sequence of their promoter regions. The expression of nifH-gusA fusions is induced under microaerobic conditions and is dependent on the presence of NifA.Abbreviations bp
base pair(s)
- kb
kilobase(s)
- ORF
open reading frame 相似文献
107.
108.
N. Islam E. J. Evans 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1994,88(5):530-534
The effects of lodging and nitrogen rate were studied in a field trial of oilseed rapeBrassica napus L. Lodging decreased seed yield (16%) compared with a frame-raised crop. Yield decreased because of a significant reduction in each of the yield components coupled with a reduced plant population caused by stem breakage at the ground level. Lodging also reduced the final crop dry weight and harvest index. Seed yield was also lower when 200 kg ha–1 nitrogen was applied than with 400 kg ha–1. A general decrease in pod number m–2, seed nuber pod–1 and seed weight caused the lower yields. The use of 400 kg ha–1 of nitrogen changed the contribution of the terminal raceme and individual branches with respect to seed yield. Seed nitrogn content and nitrogen yield increased at the 400 kg ha–1, lowering both seed oil content and oil yield. 相似文献
109.
Niels Kroer 《FEMS microbiology ecology》1994,13(3):217-224
Abstract Cell volume, carbon and nitrogen content were determined for bacteria grown in batch cultures in water samples collected at five localities in western Florida, USA. Cultures were set up by inoculating 0.2 μm filtered water with 2.5 to 7.0% of 1.0 μm filtered water. Biovolumes of the bacteria were measured by epifluorescence photomicrography. Bacterial carbon and nitrogen contents were determined with a CHN analyser. During incubations, bacterial volumes doubled from 0.070±0.037 μ m3 (mean ± S.E.) to 0.153 ± 0.036 μ m3 at early stationary phase. Bacterial C:N ratios ranged between 2.8 and 10.3, with a mean of 6.5, and were inversely correlated with cell volumes. Conversion factors for volume to carbon and nitrogen content were relatively high and variable, ranging from 0.21 to 161 pg C μm−3 (mean: 0.72 pg C μm−3 ) and from 0.05 to 0.25 pg N μm−3 (mean: 0.12 pg N μm−3 ). Small cells contained more C and N per unit volume than did large cells. The data suggested that biovolume to biomass conversion factors may be higher than previously thought and may be highly variable both temporally and geographically. 相似文献
110.
Xylem-tapping mistletoes transpire large volumes of water (E) while conducting photosynthesis (A) at low rates, thus maintaining low instantaneous wateruse efficiency (A/E). These gas-exchange characteristics have been interpreted as a means of facilitating assimilation of nitrogen dissolved at low concentration in host xylem water; however, low A/E also results in substantial heterotrophic carbon gain. In this study, host trees (Juniperus osteosperma) were fertilized and gas exchange of mistletoe (Phoradendron juniperinum) and host were monitored to determine whether mistletoe A/E would approach that of the host if mistletoes were supplied with abundant nitrogen. Fertilization significantly increased foliar N concentrations (N), net assimilation rates, and A/E in both mistletoe and host. However, at any given N concentration, mistletoes maintained lower A and lower A/E than their hosts. On the other hand, when instantaneous water-use efficiency and A/N were calculated to include heterotrophic assimilation of carbon dissolved in the xylem sap of the host, both water-use efficiency and A/N converged on host values. A simple model of Phoradendron carbon and nitrogen budgets was constructed to analyze the relative benefits of nitrogen- and carbonparasitism. The model assumes constant E and includes feedbacks of tissue nitrogen concentration on photosyn-thesis. These results, combined with our earlier observation that net assimilation rates of mistletoes and their hosts are approximately matched (Marshall et al. 1994), support part of the nitrogen-parasitism hypothesis: that high rates of transpiration benefit the mistletoe primarily through nitrogen gain. However, the low ratio of A/E is interpreted not as a means of acquiring nitrogen, but as an inevitable consequence of an imbalance in C and N assimilation.This research was supported by the National Science Foundation (grants BSR-8706772 and 8847942). 相似文献