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991.
SUMMARY 1. Some predatory zooplankton, such as the large cladoceran Bythotrephes longimanus (Leydig), have a large compound eye, hence vision may play an important role in prey detection and encounter. Our objectives were to quantify, under different light regimes, the reaction distance of Bythotrephes to an assortment of zooplankton prey and to model encounter rate with prey from Harp Lake, Ontario. Reaction distance to prey increased at higher light intensity.
2. Results from the encounter model show that small, slow-moving prey faced the greatest risk from Bythotrephes and most encounters occurred in the upper 10 m of the water column throughout the 24-h period. The model was highly sensitive to ambient light. Encounter rate and prey risk were two to three orders of magnitude more sensitive to swimming velocity of the predator than to that of the prey. 相似文献
2. Results from the encounter model show that small, slow-moving prey faced the greatest risk from Bythotrephes and most encounters occurred in the upper 10 m of the water column throughout the 24-h period. The model was highly sensitive to ambient light. Encounter rate and prey risk were two to three orders of magnitude more sensitive to swimming velocity of the predator than to that of the prey. 相似文献
992.
993.
994.
Yuferov V. P. Culpepper-Morgan J. A. LaForge K. S. Ho A. Kreek M. J. 《Neurochemical research》1998,23(4):505-511
The endogenous opioid peptide dynorphin has been shown by immunochemical studies to be widely distributed in the gastrointestinal tract. The aim of this study was to determine basal levels of preprodynorphin (ppDyn) mRNA in different regions of the gastrointestinal tract of the guinea pig. A modified sensitive and specific solution hybridization RNase protection assay was used to quantitate ppDyn mRNA, with confirmation by gel analysis of the RNase protected hybrids and PCR amplified cDNA. This method combines high sensitivity and sufficient throughput to analyze large number of samples in a single assay. Low but measurable amounts of ppDyn mRNA were detected in fundus, duodenum, jejunum, ileum, cecum, and rectum. The rectum contained significantly more ppDyn mRNA than the stomach, small bowel, and cecum. The muscularis/myenteric plexus layer of both ileum and rectum contained a higher concentration of ppDyn mRNA per g total RNA compared to the mucosa/submucosa/submucosal plexus. However, a greater absolute amount of ppDyn mRNA (80–85%) localized to the mucosal layer. The greater absolute amount of ppDyn mRNA in the mucosal layer may indicate the presence of dynorphin in the endocrine cells of the mucosa. 相似文献
995.
996.
HUnter D. M. and Webster J. M. 1973. Determination of the migratory route of botfly larvae, Cuterebra grisea (Diptera: Cuterebridae) in deermice. International Journal for Parasitology3:311–316. Cuterebra grisea larvae introduced into deermice (Peromyscus manicutatus) occurred in the nasal passages (nose or eye entry) or were associated with the esophagus and trachea (mouth entry) in the first three days after entry. During this period, some deermice exhibited a violent wheezing reaction. From day 4 to 6 after entry, larvae migrated to the final development site in the inguinal region of the deermouse. Migrating larvae were found on the front and top of the head, on the back and at the base of the tail. The similarity of this migratory path for C. grisea with the final development sites of other Cuterebra species is discussed. 相似文献
997.
Taichi Ikedo Manabu Minami Hiroharu Kataoka Kosuke Hayashi Manabu Nagata Risako Fujikawa Fumiyoshi Yamazaki Mitsutoshi Setou Masayuki Yokode Susumu Miyamoto 《Biochemical and biophysical research communications》2018,495(1):332-338
Object
The wall thickness of intracranial aneurysms (IAs) is heterogeneous. Although thinning of the IA wall is thought to contribute to IA rupture, the underlying mechanism remains poorly understood. Recently, imaging mass spectroscopy (IMS) has been used to reveal the distribution of phospholipids in vascular diseases. To investigate the feature of phospholipid composition of IA walls, we conducted IMS in a rat model of experimentally induced IA.Material and methods
IAs were surgically induced in 7-week-old male rats and analyzed by IMS in negative-ion mode.Results
A molecule at m/z 885.5 was more abundant in the thickened wall than in the thinned wall (P = 0.03). Multiple-stage mass spectroscopy revealed the molecule to be phosphatidylinositol containing stearic acid and arachidonic acid (PI 18:0/20:4). Immunohistochemistry indicated that vascular smooth muscle cells (SMCs) in the thickened wall had dedifferentiated phenotypes. To investigate the relationship between accumulation of PI (18:0/20:4) and phenotypic changes in SMCs, we subjected primary mouse aortic SMCs to liquid chromatography–tandem mass spectrometry. Notably, dedifferentiated SMCs had 1.3-fold more PI (18:0/20:4) than partly differentiated SMCs.Conclusions
Our study demonstrated the heterogeneity in phospholipid composition of the aneurysmal walls using experimentally induced IAs. PI (18:0/20:4) accumulated at high levels in the thickened aneurysmal wall where synthetic dedifferentiated SMCs exist, suggesting that this phospholipid may be involved in the phenotypic switching of medial SMCs in the IA wall. 相似文献998.
Alexandra J. Townsend Francesco Saccon Vasco Giovagnetti Sam Wilson Petra Ungerer Alexander V. Ruban 《BBA》2018,1859(9):666-675
Non-photochemical quenching (NPQ) of chlorophyll fluorescence is the process by which excess light energy is harmlessly dissipated within the photosynthetic membrane. The fastest component of NPQ, known as energy-dependent quenching (qE), occurs within minutes, but the site and mechanism of qE remain of great debate. Here, the chlorophyll fluorescence of Arabidopsis thaliana wild type (WT) plants was compared to mutants lacking all minor antenna complexes (NoM). Upon illumination, NoM exhibits altered chlorophyll fluorescence quenching induction (i.e. from the dark-adapted state) characterised by three different stages: (i) a fast quenching component, (ii) transient fluorescence recovery and (iii) a second quenching component. The initial fast quenching component originates in light harvesting complex II (LHCII) trimers and is dependent upon PsbS and the formation of a proton gradient across the thylakoid membrane (ΔpH). Transient fluorescence recovery is likely to occur in both WT and NoM plants, but it cannot be overcome in NoM due to impaired ΔpH formation and a reduced zeaxanthin synthesis rate. Moreover, an enhanced fluorescence emission peak at ~679?nm in NoM plants indicates detachment of LHCII trimers from the bulk antenna system, which could also contribute to the transient fluorescence recovery. Finally, the second quenching component is triggered by both ΔpH and PsbS and enhanced by zeaxanthin synthesis. This study indicates that minor antenna complexes are not essential for qE, but reveals their importance in electron stransport, ΔpH formation and zeaxanthin synthesis. 相似文献
999.
We herein report the design, synthesis and molecular docking studies of 2,4-thiazolidinedione derivatives containing benzene sulphonyl group which are docked against the Peroxisome Proliferator Activated Receptor (PPARγ) target. Compound 7p was most effective in lowering the blood glucose level as compared to standard drugs pioglitazone and rosiglitazone. Compound 7p exhibited potent PPAR-γ transactivation of 61.2% with 1.9 folds increase in gene expression. In molecular docking studies 7p showed excellent interactions with amino acids TYR 473, SER 289, HIE 449, TYR 327, ARG 288, MET 329 and LEU 228. Compound 7p did not cause any damage to the liver without any noteworthy weight gain and may be considered as promising candidates for the development of new antidiabetic agents. 相似文献
1000.
An improved, forthright, and highly efficient one-pot synthesis of a wide range of pharmaceutically exciting diverse kind of functionalized 2-amino-3-cyano-4H-pyrans and especially bis-pyrans compounds is developed using piperazine as a low-cost and environmentally benign commercially available basic catalyst in aqueous media. The attractive features of this process are simple procedure, short reaction times, high yields, no column chromatographic separation and commercial availability and recyclability of the catalyst. Also, piperazine can catalyze the synthesis of the target compounds on a larger scale. Furthermore, rational mechanism was suggested via GC-Mass analysis of the trapped intermediates. 相似文献