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51.
Plasmid transfer to indigenous marine bacterial populations by natural transformation 总被引:3,自引:0,他引:3
Abstract Horizontal gene transfer among microbial populations has been assumed to occur in the environment, yet direct observations of this phenomenon are rare or limited to observations where the mechanism(s) could not be explicitly determined. Here we demonstrate the transfer of exogenous plasmid DNA to members of indigenous marine bacterial populations by natural transformation, the first report of this process for any natural microbial community. Ten percent of marine bacterial isolates examined were transformed by plasmid DNA while 14% were transformed by chromosomal DNA. Transformation of mixed marine microbial assemblages was observed in 5 of 14 experiments. In every case, acquisition of the plasmid by members of the indigenous flora was accompanied by modification (probably from genetic rearrangement or methylation) that altered its restriction enzyme digestion pattern. Estimation of transformation rates in estuarine environments based upon the distribution of competency and transformation frequencies in isolates and mixed populations ranged from 5 × 10−4 to 1.5 transformants/1 day. Extrapolation of these rates to ecosystem scales suggests that natural transformation may be an important mechanism for plasmid transfer among marine bacterial communities. 相似文献
52.
Ingrid Ahrenholtz Michael G. Lorenz Wilfried Wackernagel 《Archives of microbiology》1994,161(2):176-183
A quantitative endonuclease assay, which relies on the introduction of single and double strand breaks into supercoiled plasmid DNA, was used to study the activity of the extracellular nuclease of Serratia marcescens SM6 in buffer and in groundwater. The parallel enzyme concentration-dependent production of relaxed and linear plasmid molecules suggests that the nuclease produces single and double strand breaks in duplex DNA. Bovine serum albumin stimulated the nuclease activity towards DNA and RNA and increased the stability of the enzyme against thermal inactivation. The DNase activity at 4 °C and 50 °C was almost half of that at the optimum temperature (37 °C). The nuclease was active in groundwater, although the specific activity was lower than in buffer. In a groundwater aquifer microcosm, mineral-adsorbed transforming DNA was substantially less accessible to the nuclease than was dissolved DNA. The data suggest that the extracellular nuclease of Serratia marcescens may contribute to DNA turnover in the environment and that adsorption of DNA to minerals provides protection against the nuclease.Abbreviations GW groundwater
GWA groundwater aquifer 相似文献
53.
Labidocera aestiva and L. scotti were found in the Tamiahua Lagoon, Veracruz, Mexico. Fleminger (1957) found that the populations of these species may overlap geographically, although L. aestiva has affinity to the Carolinian province and L. scotti to the Caribbean province. This study describes the seasonal behavior and succession of this species in the Tamiahua Lagoon, a brackish water system with a high marine influence. A qualitative and quantitative analysis of samples was made in March, July, and September 1985 and January 1986. L. aestiva was found in temperatures below 26 °C in a wide salinity range. At temperatures above 26 °C and up to 32 °C, L. aestiva was present also with euryhaline character. In the Tamiahua Lagoon these two species did not overlap during this study. Both species are considered temporary inhabitants of this estuarine system in the Western Gulf of Mexico. 相似文献
54.
Individual trees, either of the same or different species, can be linked spatially and temporally by the hyphae of ectomycorrhizal
(ECM) fungi that allow carbon and nutrients to pass among them and promote forest establishment following disturbance. Spatial
and temporal linkages between plants influence the function of ECM fungi in the field. Studies indicate that ECM linkages
can reduce plant competition for resources, promote forest recovery, and influence the pattern of plant succession. The degree
of influence depends on many factors, including the composition and arrangement of the vegetative community and soil and climatic
conditions. Management practices that create intense disturbance and loss of organic matter or promote the introduction of
non-ectomycorrhizal host species can decrease the ability of plants to form linkages with ECM fungi. Management practices
that retain living trees and shrubs and input of organic matter provide the energy source and substrate necessary for ECM
linkages. More research is needed to determine the degree to which ECM fungal linkages occur in the field and their role in
ecosystem function and long-term health. 相似文献
55.
56.
本文给出天然林内红松种群年令更替数学模型(1)。通过对(1)进行定性分析,得到主要结论是:系统(1)在第一象限内存在唯一稳定周期解的充要条件是bk-bc-2d>0其生态意义是天然林内红松幼树与母树随时间变化会产生一个有规律、互为消涨的变化特征。 相似文献
57.
自然免耕下的稻田生态系统 总被引:15,自引:0,他引:15
自然免耕是一种新型的稻田耕作法,通过人为改变地表微地形,建立了一个适合水旱作物复种轮作,鱼、萍、鸭周年共生的稻田生态系统.自然免耕促进了土壤内、外环境的物质、能量和信息交换,水、热、气、肥谐调.并通过环境网络效应,强化了环境与生物区系间的缓冲-调节力.提高了土壤自调能力和土壤有序度. 相似文献
58.
促进兴安落叶松天然更新的出苗条件研究 总被引:7,自引:0,他引:7
根据40多块标准地调查,促进更新措施后兴安落叶松1年生幼苗出现的频度及数量与种子年关系很大,幼苗更新效果最好的是种子年当年,更新频度可达60%以上,幼苗平均为1.0—2.0×103株·ha-1,其他年份促进的效果较差.同时,也与迹地类型、种源状况和整地质量关系较大.一般在山坡中、下部,土壤湿润的杜香落叶松和藓类越桔落叶松林迹地促进效果较好,其更新频度为60—70%.绝大部分更新幼苗出现在表土裸露、无杂草灌木、土壤湿润的地方.更新频度与植被盖度呈明显的衰减指数相关.促进地块距下种林墙最好不超过60m范围. 相似文献
59.
Max L. Bothwell Darren Sherbot Andre C. Roberge Ralph J. Daley 《Journal of phycology》1993,29(1):24-35
Growth rates, accumulation dynamics, and species succession of periphytic diatom communities were examined in the presence and absence of natural ultraviolet (UV) radiation using a series of outdoor, continuous-flow experimental flumes located on the South Thompson River, British Columbia. In a short-term experiment (2–3 wk), log-phase growth rates of naturally seeded diatom communities comprised of Tabellaria fenestrata (Lyngb.) Kütz., T. flocculosa (Roth) Kütz., Fragilaria crotonesis Kitton, and F. vaucheriae (Ehr.) Peter. exposed to 90% ambient photosynthetically active radiation (PAR) + UV were 30–40% lower than growth rates under 90% PAR alone. UV inhibition of growth rate was independent of the degree of P limitation within the range of relative specific growth rates (μ:μmax-P) of 0.5–1.0. In a long-term trial, inhibition of attached diatom accumulation under 90% PAR + UV during the first 2–3 wk was corroborated. Reduction of full sunlight to 50% PAR + UV prevented the initial inhibition phase. The initial inihibitory effect of 90% PAR + UV on algal accumulation was reversed after 3–4 wk, and by 5 wk total diatom abundance (chlorophyll a, cell numbers and cell biovolumes) in communities exposed to PAR + UV were 2–4-old greater than in communities protected from UV. Under 90% PAR + UV and 50% PAR + UV, a succession to stalked diatom genera (Cymbella and Gomphoneis) occurred. Species succession under UV radiation doubled the mean cell size of the diatom communities. The shift from inhibition to a long-term increase in the autotrophic community under PAR + UV compared to PAR alone provides further evidence against the use of short-term incubation experiments to define the long-term implications of increases in UVB. These results suggest that the ecological effects of present-day levels of UVB and UVB:UVA ratios on autotrophic communities are not well understood and might be mediated through complex trophic level interactions. 相似文献
60.
An ecosystems approach to base-rich freshwater wetlands,with special reference to fenlands 总被引:1,自引:1,他引:0
Geert van Wirdum 《Hydrobiologia》1993,265(1-3):129-153
A survey of base-rich wetlands in The Netherlands is presented. The main area of their occurrence is the low-lying Holocene
part of the country, until some thousand years ago a large and coherent wetland landscape: the Holland wetland. The development
of various parts of the Holland wetland into marshes, fens and bogs can be understood from hydrological relations in mire
basins, as recognized in the distinction of primary, secondary and tertiary mire basin stages. Presently, the remnants of
the Holland wetland are separate base-rich wetlands. The succession of their vegetation reflects various abiotic conditions
and human influences. Three main developmental periods are distinguished as regards these factors. The first, geological period
of mire development is seen as a post-glacial relaxation, with the inertia due to the considerable mass of wetland as a stabilizing
factor. Biological “grazing” influences, as an aspect of utilization by humans, converted base-rich wetlands to whole new
types in the second, historical period. Presently, mass and harvesting have decreased in importance, and actual successions
in terrestrializing turbaries seem to reflect rapidly changing environmental conditions. Human control could well become the
most important factor in the future development of wetland nature. The present value of open fen vegetation strongly depends
on the continuation of the historical harvesting. The development of wooded fen may help to increase the mass of wetland in
the future. Best results in terms of biodiversity are expected when their base state is maintained through water management.
The vegetation and hydrology of floating fens in terrestrializing turbaries is treated in some more detail. Various lines
and phases in the succession are distinguished. Open fen vegetation at base-rich, yet nutrient-poor sites is very rich in
species threatened elsewhere. The fast acidification of certain such fens is attributed to hydrological and management factors.
This acidification is illustrated in the profile of a floating raft sample. At the scale of these small fens, the elemental
structure comprising base-rich fen, transitional fen and bog vegetation, is not as stable as it was in the large Holland wetland.
A critical role seems to be played by the supply of bases with the water influx. The changing base state is supposed to change
the nutrient cycling to such an extent that it would be correct to call this trophic excitation of the ecosystem, rather than
just eutrophication. Eutrophication indicates a quantitative reaction to an increased nutrient supply, the internal system
being unaltered. The drainage of fens, resulting in an increased productivity of the vegetation, provides another example
of excitation, to the effect that the functional system is dramatically changed internally. 相似文献