全文获取类型
收费全文 | 18751篇 |
免费 | 1040篇 |
国内免费 | 801篇 |
专业分类
20592篇 |
出版年
2023年 | 304篇 |
2022年 | 403篇 |
2021年 | 519篇 |
2020年 | 465篇 |
2019年 | 681篇 |
2018年 | 657篇 |
2017年 | 432篇 |
2016年 | 425篇 |
2015年 | 522篇 |
2014年 | 1207篇 |
2013年 | 1372篇 |
2012年 | 819篇 |
2011年 | 1088篇 |
2010年 | 821篇 |
2009年 | 853篇 |
2008年 | 901篇 |
2007年 | 928篇 |
2006年 | 817篇 |
2005年 | 690篇 |
2004年 | 635篇 |
2003年 | 511篇 |
2002年 | 448篇 |
2001年 | 318篇 |
2000年 | 291篇 |
1999年 | 302篇 |
1998年 | 246篇 |
1997年 | 238篇 |
1996年 | 239篇 |
1995年 | 229篇 |
1994年 | 203篇 |
1993年 | 177篇 |
1992年 | 178篇 |
1991年 | 177篇 |
1990年 | 128篇 |
1989年 | 131篇 |
1988年 | 105篇 |
1987年 | 105篇 |
1986年 | 77篇 |
1985年 | 194篇 |
1984年 | 245篇 |
1983年 | 180篇 |
1982年 | 207篇 |
1981年 | 186篇 |
1980年 | 191篇 |
1979年 | 132篇 |
1978年 | 112篇 |
1977年 | 117篇 |
1976年 | 130篇 |
1975年 | 87篇 |
1974年 | 76篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
41.
The effects of human red cell glycophorin A (GPA) on the translocation to the plasma membrane and anion transport activity of the human erythrocyte anion transporter (band 3; AE1) have been examined using the Xenopus oocyte expression system. We show that band 3 accumulates steadily at the oocyte surface with time in the presence or absence of GPA, but this occurs more quickly when GPA is coexpressed. The amount of band 3 at the surface is determined by the concentrations of band 3 and GPA cRNA that are injected, with a higher proportion of total band 3 being translocated to the surface in the presence of GPA cRNA. The increased expression of DNDS-sensitive chloride transport is highly specific to GPA, and is not observed when the cRNA to the putative glycophorin E or a very high concentration of the cRNA to glycophorin C are coexpressed with band 3 in oocytes.We thank Dr. Kay Ridgwell and Charlotte Ratcliffe for supplying plasmids and Dr. David Anstee for antibodies. This work was supported by grants from the Medical Research Council. 相似文献
42.
D. James Morré 《Journal of bioenergetics and biomembranes》1994,26(4):421-433
An NADH oxidase activity of animal and plant plasma membrane is described that is stimulated by hormones and growth factors. In plasma membranes of cancer cells and tissues, the activity appears to be constitutively activated and no longer hormone responsive. With drugs that inhibit the activity, cells are unable to grow although growth inhibition may be more related to a failure of the cells to enlarge than to a direct inhibition of mitosis. The hormone-stimulated activity in plasma membranes of plants and the constitutively activated NADH oxidase in tumor cell plasma membranes is inhibited by thiol reagents whereas the basal activity is not. These findings point to a thiol involvement in the action of the activated form of the oxidase. NADH oxidase oxidation by Golgi apparatus of rat liver is inhibited by brefeldin A plus GDP. Brefeldin A is a macrolide antibiotic inhibitor of membrane trafficking. A model is presented where the NADH oxidase functions as a thiol-disulfide oxidoreductase activity involved in the formation and breakage of disulfide bonds. The thiol-disulfide interchange is postulated as being associated with physical membrane displacement as encountered in cell enlargement or in vesicle budding. The model, although speculative, does provide a basis for further experimentation to probe a potential function for this enzyme system which, under certain conditions, exhibits a hormone- and growth factor-stimulated oxidation of NADH. 相似文献
43.
44.
Roots of ten-days-old seedlings obtained from a maize hybrid grown in complete or in sulphate-deprived medium were used to extract Poly(A)+RNA. The response to sulphate deprivation, which is known to increase the uptake capacity up to ten times, was manifested also by the expression of three mRNA species, as shown by the in vitro translation of the mRNA population. One hour after transfer to complete nutrient medium all three mRNAs were still present.Abbreviations BSA
Bovine Serum Albumine
- DTT
1,4-Dithio-DL-Threitol
- EDTA
Ethylenediaminetetraacetic-acid
- MES
2-(N-Morpholino) ethane sulfonic acid
- SDS
Sodium Dodecyl Sulfate
- TCA
Trichloracetic acid
- TRIS
Tris(hydroxy-methyl)-aminomethane 相似文献
45.
Kumar B. Reddy Barbara A. Hocevar Philip H. Howe 《Journal of cellular biochemistry》1994,56(3):418-425
Transforming growth factor β1 (TGFβ1) inhibits epithelial cell proliferation late in the G1 phase of the cell cycle. We examined the effect of TGFβ1 on known late G1 cell cycle regulators in an attempt to determine the molecular mechanism of growth inhibition by this physiological inhibitor. The results demonstrate the TGFβ1 inhibits the late G1 and S phase specific histone H1 kinase activity of p33cdk2. This inhibitiion is not dur to TGFβ1's effect on p33cdk2 synthesis, but rather due to its negative effect on the late G1 phosphorylation of p33cdk2. It is also shown that TGFβ1 inhibits both late G1 cyclin A and cyclin E associated histon H1 kinase activities. The inhibitor has no effects on the synthesis of cyclin E but to inhibit the synthesis of cyclin A protein in a cell cycle dependent manner. If TGFβ1 is added to cells which have progressed futher than 8 hours into G1, then it is without inhibitory effect on cyclin A synthesis. These effect on TGFβ1 on late G1 cell cycle regulators correlate well with its inhibitory effects on cellular growth and suggest that these G1 cyclin dependent kinases might serve as targets for TGFβ1-mediated growth arrest. 相似文献
46.
47.
L. O. Greenfield 《Human Evolution》1993,8(3):187-204
A tooth at the border between two morphogenetic fields (mandibular canine and honing premolar) may become morphologically
similar to and/or functionally incorporated with the teeth of either field. In light of this, observations of the morphology
and occlusion of female anthropoid C1s from 58 extant species are presented to assess whether and to what extent they exhibit incisor-like form and function. Female
C1s in 74% of the taxa observed exhibit well developed incisor-like traits which may reflect field border phenomena. In another
9%, incisor traits are present but they are not examples of field border phenomena. Interspecies variation in female C1 morphology is related to behavior, function, natural selection and phyletic inertia. A selection model, derived from the
data, is used to explain C1 sexual dimorphism and the evolution of male and female human canines. The data's relevance to the field vs clone theory debate
is also discussed. 相似文献
48.
49.
Backgroundobesity is a major risk factor for many metabolic diseases such as diabetes and cardiometabolic diseases. This study aimed to evaluate the association of plasma and urinary barium concentrations, CYP19A1 gene polymorphisms, and their interaction with central obesity in a rural Chinese population.Methodsrestricted cubic spline model was used to explore the dose-response relationship between barium and the risk of developing central obesity and waist circumference; logistic regression model was used to assess the association between barium, CYP19A1 gene polymorphisms and their interaction with central obesity.Resultsthe results of the restricted cubic spline model showed that plasma barium concentration was linearly associated with the risk of developing central obesity and non-linearly associated with waist circumference. Logistic regression analysis showed that participants with Q4 plasma barium concentration exhibited a higher risk of central obesity compared to participants with Q1 barium concentration; participants carrying the rs10046-AA gene exhibited a lower risk of central obesity than those carrying the rs10046-G(GG+GA) gene; participants carrying the rs10046-GA genotype showed 1.754 times higher risk of central obesity than those carrying rs10046-GG+AA genotype. There was a significant interaction between plasma barium and CYP19A1 gene polymorphism on central obesity.Conclusionthe development of central obesity was associated with plasma barium and CYP19A1. 相似文献
50.
Absorption and imagery locate immune responses in the body 总被引:2,自引:0,他引:2
M. Banks Gregerson Ingram M. Roberts Michael M. Amiri 《Applied psychophysiology and biofeedback》1996,21(2):149-165
Imagery instructions specifying mucosal immunity should alter mucosal immunoglobulin A (m-IgA) levels in high absorbers, whose intent concentration evokes intense physiological responses. After screening for health status, 121 high or low absorbers were randomly assigned to either Relaxation Alone (R), Relaxation with Mucosal Immune Imagery (RI), or Vigilance Task control (VT). Before and after one 60-min intervention, subjects reported theory-relevant psychological variables and provided 5ml whole saliva, which was immediately frozen and assayed lateren masse with enzyme-linked immunoabsorbence (ELISA). MANOVA analysis of psychological variables replicated past research. ANOVA on residualized m-IgA found Time × Absorption interaction and Condition main effects. High more than low absorbers responded to relaxation with mucosal immune imagery by producing higher m-IgA. High absorbers appear able to locate where their immune systems will respond. Individual differences like absorption level need to be emphasized in diagnosis and treatment responsiveness.National Institutes of HealthM. Banks (Jasnoski) Gregerson, Department of Psychology, The George Washington University, changed to The Family Therapy Institute; Ingram M. Roberts, The George Washington University Medical Center, changed to Department of Medicine, Bridgeport Hospital; and Michael M. Amiri, The George Washington University Medical Center, changed to the Department of Neuroscience, NINDS Branch, National Institutes of Health. This research supported by an intra-mural BioMedical Research Grant from The George Washington University, was presented at the 1992 Annual Meeting of the Eastern Psychological Association, Boston, Massachusetts. Special thanks are extended to the following students who assisted instrumentally at various stages: undergraduates Lina Alathari, S. Theodor King, Beth Lieberman, Parisa Lotfi, Anita McClenon, and Karen Siscoe, and graduate student Mariken Hasert. 相似文献