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171.
The twigs of Dorstenia barteri var. subtriangularis yielded three diprenylated chalcones: (-)-3-(3,3-dimethylallyl)-5'-(2-hydroxy-3-methylbut-3-enyl)-4,2',4'-trihydroxychalcone, (+)-3-(3,3-dimethylallyl)-4',5'-[2'-(1-hydroxy-1-methylethyl)-dihydrofurano]-4,2'-dihydroxychalcone and 3,4-(6",6"-dimethyldihydropyrano)-4',5'-[2',-(1-hydroxy-1-methylethyl)-dihydrofurano]-2'-hydroxychalcone for which the names bartericins A, B and C, respectively, are proposed. Stipulin, beta-sitosterol and its 3-beta-D-glucopyranosyl derivative were also isolated. The structures of these secondary metabolites were determined on the basis of spectroscopic analysis, especially, NMR spectra in conjunction with 2D experiments, COSY, HMQC and HMBC. The structural relationship of bartericins B and C was further established by the chemical cyclization of one to the other. 相似文献
172.
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174.
川楝子中两个新的苯丙三醇甙 总被引:7,自引:0,他引:7
从川楝子(fruitsofMeliatoosendanSieb.etZucc.)的水溶性成分中分离出两个新的苯丙三醇甙:川楝甙A(3-甲氧基-5-羟基-9-(1’-O-β-D-葡萄糖)-苏式-苯丙三醇)和川楝甙B(4-羟基-7,8-(2’,1’-O-β-D-葡萄糖)-苯丙三醇);同时首次分离出苏式-愈创木基甘油。通过波谱解析和化学方法确定了它们的结构。 相似文献
175.
大仓鼠的核型与B染色体研究 总被引:1,自引:0,他引:1
采用骨髓细胞染色体制片法,对分布于山东济南、泰山、东北长白山和陕西西安的大仓鼠的染色体组型、G-带、C-带和银染核型进行了分析研究。济南、西安和长白山的标本的二倍体数目和核型相似,2n=28,22t+4m+XY(st,m)。泰山标本的二倍体数目为2n=28~29,即在675%的中期相中多出了一条形态最小的端着丝粒染色体,这条染色体为B染色体,可能起源于X染色体。泰山标本的A染色体组与上述3地标本相同。4地标本的G-带、C-带和银染核型相似。除B染色体外,每个端着丝粒染色体都具有着丝粒异染色质,AgNORs较恒定地出现在Nos2,4,8,9,13染色体上。也就是说大仓鼠的B染色体为C-带阴性,不携带核仁组织者。这种B染色体C-带阴性的特征在赤狐、黑家鼠和大林姬鼠朝鲜亚种中亦有报道。 相似文献
176.
Gjerset RA 《Journal of molecular histology》2006,37(5-7):239-251
The p53/p14ARF/mdm2 stress response pathway plays a central role in mediating cellular responses to oncogene activation, genome instability, and therapy-induced DNA damage. Abrogation of the pathway occurs in most if not all cancers, and may be essential for tumor development. The high frequency with which the pathway is disabled in cancer and the fact that the pathway appears to be incompatible with tumor cell growth, has made it an important point of focus in cancer research and therapeutics development. Recently, Nucleophosmin (NPM, B23, NO38 and numatrin), a multifunctional nucleolar protein, has emerged as a p14ARF binding protein and regulator of p53. While complex formation between ARF and NPM retains ARF in the nucleolus and prevents ARF from activating p53, DNA damaging treatments promote a transient subnuclear redistribution of ARF to the nucleoplasm, where it interacts with mdm2 and promotes p53 activation. The results add support to a recently proposed model in which the nucleolus serves as a p53-uspstream sensor of stress, and where ARF links nucleolar stress signals to nucleoplasmic effectors of the stress response. A better understanding of ARF’s nucleolar interactions could further elucidate the regulation of the p53 pathway and suggest new therapeutic approaches to restore p53 function. 相似文献
177.
Indirect competitive immunoassay for detection of aflatoxin B1 in corn and nut products using the array biosensor 总被引:1,自引:0,他引:1
Sapsford KE Taitt CR Fertig S Moore MH Lassman ME Maragos CM Shriver-Lake LC 《Biosensors & bioelectronics》2006,21(12):2298-2305
Because of the potential health risks of aflatoxin B1 (AFB1), it is essential to monitor the level of this mycotoxin in a variety of foods. An indirect competitive immunoassay has been developed using the NRL array biosensor, offering rapid, sensitive detection and quantification of AFB1 in buffer, corn and nut products. AFB1-spiked foods were extracted with methanol and Cy5-anti-AFB1 added to the resulting sample. The extracted sample/antibody mix was passed over a waveguide surface patterned with immobilized AFB1. The resulting fluorescence signal decreased as the concentration of AFB1 in the sample increased. The limit of detection for AFB1 in buffer, 0.3 ng/ml, was found to increase to between 1.5 and 5.1 ng/g and 0.6 and 1.4 ng/g when measured in various corn and nut products, respectively. 相似文献
178.
Artoni RF Vicari MR Endler AL Cavallaro ZI de Jesus CM de Almeida MC Moreira-Filho O Bertollo LA 《Genetica》2006,127(1-3):277-284
B chromosomes in Prochilodus lineatus, a migratory neotropical fish, were analyzed in a comparative study among populations from the Dourada lagoon (State of Paraná, Brazil) and from Mogi-Guaçu river (State of São Paulo, Brazil). The data on C-banding and fluorescent in situ hybridization with a satellite DNA probe (SATH1), indicate that the small metacentric B chromosome might correspond to an isochromosome. On the other hand, both populations presented a distinct set of B chromosomes, differentiated either by their number and by the presence of variant B types in the population from Mogi-Guaçu river. The present results indicate that the B chromosomes of P. lineatus should have an ancient origin, and have undergone a differential evolutionary pathway among distinct populations. 相似文献
179.
p21HBsAg/HBsAg转基因小鼠肝脏病理学研究 总被引:3,自引:0,他引:3
目的观察p21HBsAg/HBsAg转基因小鼠肝脏病理学改变.方法分别选取2、6、12、18、24月龄的SPF级p21HBsAg/HBsAg转基因小鼠和p21+/+野生型小鼠,剖检进行大体观察,取肝脏及肝脏肿瘤组织,进行组织学HE染色及电镜超微结构观察.结果 p21HBsAg/HBsAg转基因小鼠肝脏大体、光镜和电镜下均有明显病理改变.随着月龄的增加,肝脏色暗质硬,表面有结节和肿瘤形成;光镜下,肝细胞浊肿,炎症细胞浸润,脂肪变性,点状、灶状和碎屑状坏死,非典型增生,肝细胞癌.癌细胞分化良好,类似肝细胞,形成索状和腺泡状结构.癌细胞核深染,具核分裂像.电镜下,癌细胞核变形,核膜曲折凹陷,线粒体肿胀,数目增多,嵴减少.4例18月龄转基因小鼠发生肝细胞癌(4/10),6例24月龄的转基因小鼠发生肝细胞癌(6/10),其中2例发现远处转移.结论 p21HBsAg/HBsAg转基因小鼠肝脏出现明显病理损害,18月龄小鼠开始发展成高分化的肝细胞癌,高龄小鼠形成的肝细胞癌能够转移. 相似文献
180.