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81.
Barre A Peumans WJ Menu-Bouaouiche L Van Damme EJ May GD Herrera AF Van Leuven F Rougé P 《Planta》2000,211(6):791-799
The pulp of ripe bananas (Musa acuminata) contains an abundant thaumatin-like protein (TLP). Characterization of the protein and molecular cloning of the corresponding
gene from banana demonstrated that the native protein consists of a single polypeptide chain of 200 amino acid residues. Molecular
modelling further revealed that the banana thaumatin-like protein (Ban-TLP) adopts an overall fold similar to that of thaumatin
and thaumatin-like PR-5 proteins. Although the banana protein exhibits an electrostatically polarized surface, which is believed
to be essential for the antifungal properties of TLPs, it is apparently devoid of antifungal activity towards pathogenic fungi.
It exhibits a low but detectable in vitro endo-β-1,3-glucanase (EC 3.2.1.x) activity. As well as being present in fruits,
Ban-TLP also occurs in root tips where its accumulation is enhanced by methyl jasmonate treatment of plants. Pulp of plantains
(Musa acuminata) also contains a very similar TLP, which is even more abundant than its banana homologue. Our results demonstrate for the
first time that fruit-specific (abundant) TLPs are not confined to dicots but occur also in fruits of monocot species. The
possible role of the apparent widespread accumulation of fruit-specific TLPs is discussed.
Received: 7 January 2000 / Accepted: 26 April 2000 相似文献
82.
Wang Xiao Xue-Lin Huang Xia Huang Ya-Ping Chen Xue-Mei Dai Jie-Tang Zhao 《Plant Cell, Tissue and Organ Culture》2007,90(2):191-200
A protocol for plant regeneration from protoplasts of Musa acuminata cv. Mas (AA) via somatic embryogenesis was developed. Viable protoplasts were isolated from embryogenic cell suspensions
at a yield of 1.2 × 107 protoplasts/ml packed cell volume (PCV). Liquid and feeder layer culture systems with medium-A and medium-B were used for
protoplast culture. In liquid culture system, medium-B was more efficient for inducing cell division (17.5% at 14 days) and
colony formation (6.7% at 28 days) than medium-A. However, all protoplast-derived cell colonies (PDCC) obtained from liquid
culture system could not develop further. In feeder layer culture system, there was no significant difference between medium-A
and medium-B on cell division and colony formation of the cultured protoplasts, and the cell division frequency at 14 days
and colony formation frequency at 28 days were 24.5% and 11.2%, respectively, in medium-B. Comparative study on the effects
of BAP (2.2 μM, 4.4 μM, 8.8 μM), zeatin (0.4 μM, 0.8 μM, 1.2 μM) and TDZ (0.2 μM, 0.4 μM, 0.6 μM) on embryo formation of PDCC
from feeder-layer culture indicated that TDZ was best. TDZ at 0.4 μM induced 7906 mature embryos per ml PCV PDCC, which was
4-fold the frequency as with BAP at 4.4 μM, 7.5-fold as with zeatin at 0.8 μM and 150-fold as control medium (no mentioned
cytokinins) after 45 days on M3 medium. About 44% of the mature embryos were converted into plantlets with poor root system
after subculture on M4 medium. Root further development of regenerated plantlets was promoted by addition of activated charcoal
(AC) to MS basal medium. 相似文献
83.
Markku HÄKKINEN Chee How TEO Yasmin Rofina OTHMAN 《植物分类学报》2007,45(1):69-74
Borneo is a large island in Southeast Asia. It is divided politically into three parts: the kingdom of Brunei on the north central coast; the Malaysian states of Sarawak and Sabah to the west and east; with Kalimantan of Indonesia making up the larger par… 相似文献
84.
M. A. Lysák M. Dolez˘elová J. P. Horry R. Swennen J. Dolez˘el 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(8):1344-1350
Nuclear genome size variation was studied in Musa acuminata (A genome), Musa balbisiana (B genome) and a range of triploid clones differing in genomic constitution (i.e. the relative number of A and B genomes).
Nuclear DNA content was estimated by flow cytometry of nuclei stained by propidium iodide. The A and B genomes of Musa differ in size, the B genome being smaller by 12% on average. No variation in genome size was found among the accessions
of M. balbisiana (average genome size 537 Mbp). Small, but statistically significant, variation was found among the subspecies and clones
of M. acuminata (ranging from 591 to 615 Mbp). This difference may relate to the geographical origin of the individual accessions. Larger
variation in genome size (8.8%) was found among the triploid Musa accessions (ranging from 559 to 613 Mbp). This variation may be due to different genomic constitutions as well as to differences
in the size of their A genomes. It is proposed that a comparative analysis of genome size in diploids and triploids may be
helpful in identifying putative diploid progenitors of cultivated triploid Musa clones. Statistical analysis of data on genome size resulted in a grouping which agreed fairly well with the generally accepted
taxonomic classification of Musa.
Received: 11 May 1998 / Accepted: 29 September 1998 相似文献
85.
86.
A Method for Isolation of Total RNA from Fruit Tissues of Banana 总被引:9,自引:1,他引:9
Jun-Jun Liu Chong-Jin Goh Chiang-Shiong Loh Pei Liu Eng-Chong Pua 《Plant Molecular Biology Reporter》1998,16(1):87-87
We describe a rapid and efficient method for isolation of total RNA from banana fruit tissues. The RNA was extracted with a high ionic strength buffer at room temperature. The proteins, genomic DNA and secondary metabolites in the extract were then removed by precipitation with pre-cooled potassium acetate and repeated phenol/chloroform/isoamyl alcohol extractions. The RNA was recovered by ethanol precipitation. It was relatively free of ribonucleases and was suitable for RT-PCR and northern blot analysis. The procedure can be completed in less than 4 hours. 相似文献
87.
油菜素内酯提高香蕉幼苗抗冷性的效应 总被引:5,自引:0,他引:5
冷胁迫条件下的香蕉幼苗分别经不同浓度的BR处理后,相对于对照来说,在一定的处理浓度范围内,可以明显的降低电解质外渗率,提高低温胁迫下香蕉幼苗叶片中SOD的活性,一定程度上提高了低温胁迫下香蕉幼苗叶片中可溶性蛋白的含量,减缓叶片中MDA的含量变化,可溶性糖含量明显的提高,减少叶片萎蔫面积和死亡率,同时,明显的减缓了叶片中叶绿素降解的速度。这些代谢产物的变化,对提高香蕉幼苗冷胁迫期间的抵抗能力起着非常关键和重要的作用。保护效果最好的浓度是:0.9 mg·L-1。 相似文献
88.
A promoter from sugarcane bacilliform badnavirus drives transgene expression in banana and other monocot and dicot plants 总被引:6,自引:0,他引:6
Schenk Peer M. Sagi Laszlo Remans Tony Dietzgen Ralf G. Bernard Margaret J. Graham Michael W. Manners John M. 《Plant molecular biology》1999,39(6):1221-1230
A 1369 bp DNA fragment (Sc) was isolated from a full-length clone of sugarcane bacilliform badnavirus (ScBV) and was shown to have promoter activity in transient expression assays using monocot (banana, maize, millet and sorghum) and dicot plant species (tobacco, sunflower, canola and Nicotiana benthamiana). This promoter was also tested for stable expression in transgenic banana and tobacco plants. These experiments showed that this promoter could drive high-level expression of the -glucuronidase (GUS) reporter gene in most plant cells. The expression level was comparable to the maize ubiquitin promoter in standardised transient assays in maize. In transgenic banana plants the expression levels were variable for different transgenic lines but was generally comparable with the activities of both the maize ubiquitin promoter and the enhanced cauliflower mosaic virus (CaMV) 35S promoter. The Sc promoter appears to express in a near-constitutive manner in transgenic banana and tobacco plants. The promoter from sugarcane bacilliform virus represents a useful tool for the high-level expression of foreign genes in both monocot and dicot transgenic plants that could be used similarly to the CaMV 35S or maize polyubiquitin promoter. 相似文献
89.
Deleterious effects of plant cystatins against the banana weevil Cosmopolites sordidus 总被引:1,自引:0,他引:1
Andrew Kiggundu Josephine Muchwezi Christell Van der Vyver Altus Viljoen Juan Vorster Urte Schlüter Karl Kunert Dominique Michaud 《Archives of insect biochemistry and physiology》2010,73(2):87-105
The general potential of plant cystatins for the development of insect‐resistant transgenic plants still remains to be established given the natural ability of several insects to compensate for the loss of digestive cysteine protease activities. Here we assessed the potential of cystatins for the development of banana lines resistant to the banana weevil Cosmopolites sordidus, a major pest of banana and plantain in Africa. Protease inhibitory assays were conducted with protein and methylcoumarin (MCA) peptide substrates to measure the inhibitory efficiency of different cystatins in vitro, followed by a diet assay with cystatin‐infiltrated banana stem disks to monitor the impact of two plant cystatins, oryzacystatin I (OC‐I, or OsCYS1) and papaya cystatin (CpCYS1), on the overall growth rate of weevil larvae. As observed earlier for other Coleoptera, banana weevils produce a variety of proteases for dietary protein digestion, including in particular Z‐Phe‐Arg‐MCA‐hydrolyzing (cathepsin L–like) and Z‐Arg‐Arg‐MCA‐hydrolyzing (cathepsin B–like) proteases active in mildly acidic conditions. Both enzyme populations were sensitive to the cysteine protease inhibitor E‐64 and to different plant cystatins including OsCYS1. In line with the broad inhibitory effects of cystatins, OsCYS1 and CpCYS1 caused an important growth delay in young larvae developing for 10 days in cystatin‐infiltrated banana stem disks. These promising results, which illustrate the susceptibility of C. sordidus to plant cystatins, are discussed in the light of recent hypotheses suggesting a key role for cathepsin B–like enzymes as a determinant for resistance or susceptibility to plant cystatins in Coleoptera. © 2009 Wiley Periodicals, Inc. 相似文献
90.
Béatrice Rhino Marc Dorel Philippe Tixier Jean‐Michel Risède 《Agricultural and Forest Entomology》2010,12(2):195-202
- 1 The banana weevil Cosmopolites sordidus Germar (Coleoptera: Curculionidae) is the most serious pest of banana and plantain in most production areas, including the West Indies.
- 2 During a period of 2 years, we assessed the efficacy of a pheromone mass‐trapping control strategy of C. sordidus in field conditions in Guadeloupe at different cropping stages, both in sanitation fallows and in different ratoon banana crops.
- 3 In the fallows, catches peaked 3 months after beginning trapping and then decreased to zero after 9 months. By contrast, for the new plantations, the catches of C. sordidus increased after the 11th month and, in the older banana fields, there was no decrease in C. sordidus catches. The C. sordidus catches increased in the neighbouring banana plots, whereas they decreased in the fallows, and these catches decreased with the distance from fallow.
- 4 In conclusion, mass trapping with synergized pheromone traps within fallows should allow better sanitation of banana plantations. Yet, within the farms, fallows must not be located next to new plantations to avoid massive damage to the young plants. More generally, the present study emphasizes that the control of this insect should be managed at the farm scale and not at the field scale, with special attention being paid to the location of fallows.