Cellular double-stranded RNA in Phaseolus vulgaris

Summary High molecular weight double-stranded (ds) RNAs have been detected in apparently virus-free French (common) bean Phaseolus vulgaris cv. Black Turtle Soup (BTS). Several other bean cultivars were free of detectable high molecular weight dsRNAs. The dsRNAs have been partially characterized and have homology to the BTS genome as well as to the genomes of other bean cultivars. The T _m of hybrids formed between BTS DNA and denatured dsRNA have been estimated.     

Age specificity of inbreeding depression during a life cycle ofCallosobruchus chinensis (Coleoptera: Bruchidae)

Age-specific effects of inbreeding on fecundity were assayed for adzuki bean weevil Callosobruchus chinensis by comparing inbred lines and their cross. Four consecutive full-sib matings reduced only 10.3 percent in total fecundity, and did not decrease early fecundity at all until third day from the onset of reproduction. It is suggested that recessive detrimental genes have been eliminated from the early period of adult life span when reproductive value is high. There was a slight tendency that inbreeding depression increased as age proceeded though not statistically significant.     

生长素极性运输的自动抑制

以豌豆(Pisum sativum L.)和绿豆(Phaseolus radiatus L.)为材料证明了IAA极性运输的自动抑制现象。用改进的“供体-受体技术”证明:受体中的IAAA可抑制~3H-IAA的极性运输,抑制程度随受体中加入IAA浓度的增加而增强;在“Y”型外植体一侧切口施用的IAA也可抑制~3H-IAA在另一侧的极性运输,并导致~3H-IAA在该侧组织中的积累以及代谢或钝化的增强。此外,组织中游离~3H-IAA的比例也随运输时间的延长而不断减小。     

Inhibition of Abscission of Bean Petiole Explants by Lepidimoide

The effect of lepidimoide on the process leading to abscission was studied in bean (Phaseolus vulgaris L. cv. Masterpiece) petiole explants. The assays, involving observations on the junction of the petiole of primary leaves and the pulvinus, were conducted in the light. Lepidimoide, at concentrations of 1 μm or higher, delayed the abscission process; however, the progression of abscission proceeded at normal rates, and complete abscission resulted. On the other hand indoleacetic acid inhibited the normal senescence resulting in greatly decreased abscission during the observation period. These observations show that lepidimoide only delays abscission, and the kinetics seem to indicate that lepidimoide and indoleacetic acid affect abscission through different mechanisms. Received March 1, 1996; accepted November 4, 1996