全文获取类型
收费全文 | 4923篇 |
免费 | 503篇 |
国内免费 | 142篇 |
出版年
2023年 | 105篇 |
2022年 | 105篇 |
2021年 | 175篇 |
2020年 | 148篇 |
2019年 | 194篇 |
2018年 | 158篇 |
2017年 | 169篇 |
2016年 | 181篇 |
2015年 | 200篇 |
2014年 | 286篇 |
2013年 | 347篇 |
2012年 | 193篇 |
2011年 | 265篇 |
2010年 | 139篇 |
2009年 | 210篇 |
2008年 | 229篇 |
2007年 | 256篇 |
2006年 | 195篇 |
2005年 | 145篇 |
2004年 | 170篇 |
2003年 | 140篇 |
2002年 | 97篇 |
2001年 | 83篇 |
2000年 | 85篇 |
1999年 | 86篇 |
1998年 | 60篇 |
1997年 | 83篇 |
1996年 | 71篇 |
1995年 | 70篇 |
1994年 | 73篇 |
1993年 | 68篇 |
1992年 | 60篇 |
1991年 | 44篇 |
1990年 | 40篇 |
1989年 | 39篇 |
1988年 | 45篇 |
1987年 | 43篇 |
1986年 | 58篇 |
1985年 | 49篇 |
1984年 | 38篇 |
1983年 | 20篇 |
1982年 | 45篇 |
1981年 | 32篇 |
1980年 | 45篇 |
1979年 | 36篇 |
1978年 | 27篇 |
1977年 | 33篇 |
1976年 | 25篇 |
1973年 | 22篇 |
1972年 | 24篇 |
排序方式: 共有5568条查询结果,搜索用时 15 毫秒
51.
Synopsis Aspects of the life history of Barbus anoplus were studied in Lake le Roux, a turbid man-made lake on the Orange River, South Africa. This minnow underwent a population
explosion and successfully colonized the shoreline of the newly-formed lake during the early phases of reservoir filling.
Male and female B. anoplus reach sexual maturity in one year at about 40 mm fork length. They have a multiple spawning habit with the first spawning
in November–January and the second in February–March. The growth of the two resulting cohorts is discussed. It is proposed
that the offspring from the second spawning not only acts as a ‘back-up’ but is capable of prolonging the life of that year-class
into an additional reproductive season. Most of the minnows die after their second summer, but more offspring from the second
spawning, especially females, live into a third summer. Females attain a larger maximum size (73 mm FL) and age (3–4 years)
than males (60 mm FL, 2–3 years). B. anoplus is small and short-lived with a high seasonal reproductive potential, which is in contrast to the larger Barbus species in the Orange River system. These life-history traits enable the species to colonize and successfully inhabit unstable
environments and probably account for its widespread distribution. 相似文献
52.
Multiple discriminant analysis is a useful multivariate technique in vegetation studies that can be employed for several purposes, even if the underlying statistical assumptions are not satisfied. An example of application of this method is discussed: multiple discriminant analysis was successfully used for evaluating the predictivity of forest types defined by numerical classification of vegetation data with respect to soil variables.Species nomenclature follows Pignatti (1982) for vascular plants and Augier (1966) for mosses.The research has been supported by the IDROSER (Bologna, Italy) and by the Italian C. N. R. (Grant no. 83.02548.04, resp. Prof. A. Pirola). We wish to thank Prof. E. Feoli, Prof. E. van der Maarel, Prof. L. Orlóci and Prof. A. Pirola for suggestions. We are also indebted to Dr N. Filippi who analyzed the soil samples. 相似文献
53.
The vegetation succession on the dunes near Oostvoorne, The Netherlands has been followed by means of a novel combination of repeated large-scale vegetation mapping and air photograph interpretation. Vegetation units have been discerned on the formation level because these could be distinguished fairly easily on the photographs and because the rates of change are appropriate to the time interval chosen. Nineteen formations were distinguished. Five 1:6250 maps were constructed, reflecting the formation pattern in 1934, 1943, 1959, 1972 and 1980. An overlay with 2736 grid points at 25 m field distance was used to quantify changes in the formation pattern.The results suggest a pronounced multiple pathway succession with nevertheless three principal trajectories of succession from pioneer to woodland vegetation. The outer dunes, which have originated since 1910, are distinct in successional characteristics from the inner dunes, which already existed but were released from heavy grazing pressure in 1910. The rate of change in the outer dunes was high in the beginning and is slowly decreasing eversince. In the inner dunes it went the other way around. Through visual extrapolation the likely formation patterns in 1910 and in 2000 were estimated.Transition frequencies proved highly variable for most formations. Moreover, strong spatial dependence was found. Limitations in the use of Markov models in cases of long-term succession in heterogeneous environments are discussed.Nomenclature follows the same sources as in van der Maarel et al. (1984).Field work carried out 1980–1981 when the authors were at the Division of Geobotany, University of Nijmegen. We thank Jos Rijntjes, Nijmegen, for his cooperation in the field. Vegetation maps were prepared and calculations performed at Uppsala. We thank the Foundation Het Zuid-Hollands Landschap, Rotterdam, for providing facilities and a grant for fieldwork as well as additional means to reproduce the vegetation maps. Two reviewers gave useful comments. 相似文献
54.
Functional and ultrastructural effects of nontypeable haemophilus influenzae in a hamster trachea organ culture system 总被引:1,自引:0,他引:1
Joseph M. Mylotte Richard R. Stack Timothy F. Murphy John Asirwatham Michael A. Apicella 《In vitro cellular & developmental biology. Plant》1985,21(10):575-582
Summary A hamster trachea organ culture system was utilized to evaluate quantitatively the effects of a strain of nontypeableHaemophilus influenzae (NTHI) and culture supernatants of the same strain on ciliary activity. Tracheal explants were maintained in organ culture
for 96 to 144 h and ciliary activity was observed daily with an inverted microscope. Explants continuously exposed to a strain
of NTHI had a progressive decline in ciliary activity which was significantly lower than uninfected controls evaluated concomitantly
by 48 h of exposure and thereafter. Histologic studies revealed a progressive degeneration of mucosal cells and exfoliation
of ciliated cells. Scanning electron microscopy showed little adherence of NTHI to the mucosal surface. Sterile broth cultures
of NTHI and supernatants of organ cultures infected with the same NTHI strain had no adverse effect on ciliary activity. Infected
tracheal explants treated with ampicillin 24, 48, or 72 h after continuous bacterial challenge had no significant decline
in ciliary activity compared to controls. The lack of adherence and the histologic changes observed when hamster trachea cultures
were infected with NTHI suggested a toxin might mediate the damage observed. Broth and organ culture supernatants, however,
produced no damage. Therefore, further studies are needed to determine the role, if any, of a toxin in the production of damage
to hamster tracheal explants by NTHI.
This work was supported by a Merit Review grant from the Veterans Administration and by Grant AI-19641 from the National Institute
of Allergy and Infectious Diseases. 相似文献
55.
Summary Glycoproteins rich in mannosyl or glucosyl residues were analyzed in the subcommissural organ (SCO) and the pineal organ of the sheep (Ovis aries). By use of concanavalin A labelled with fluorescein isothiocyanate, fluorescent material was found both in ependymal and hypendymal cells of the SCO. In the pineal organ, either isolated or grouped parenchymal cells showed a marked fluorescence. These cells may correspond to ependymal elements also called interstitial cells or supporting cells. In addition, scarce slender, fluorescent processes were observed in the pineal parenchyma. The techniques of electrophoresis and electrotransfer on nitrocellulose paper have been applied to analyze the glycopeptide content of the SCO and the pineal organ in comparison to cerebellar and cerebral fractions solubilized by use of Triton X 100. Approximately 30 different concanavalin A-reactive glycopeptides were revealed in each fraction. In the SCO extract four glycopeptides (30, 54, 72, 100 kd) might correspond to subunits of the glycoprotein(s) characteristically stored in the ependymal cells of the SCO. In addition, two glycopeptides (32/33, 115 kd) are specific to the pineal organ extract. The possible similarity of the concanavalin A-reactive material in both organs is discussed and a putative secretory activity of the pineal ependymal cells is postulated. 相似文献
56.
Summary Monoaminergic nerve fibers were studied in the pineal organ of the monkey, Macaca fuscata, by use of fluorescence and immunohistochemical procedures. Abundant formations of noradrenergic nerve fibers were observed in the pineal organ. They entered the parenchyma in the form of several coarse bundles via the capsule in the distal portion of the organ and spread throughout the organ after branching into smaller units. The density of the autonomic innervation decreased gradually toward the proximal portion of the organ. In the distal portion, numerous nerve fibers formed perivascular plexuses around the blood vessels and some fibers ran as bundles unrelated to the blood vessels in the stroma. Fine varicose fibers and bundles derived from these plexuses penetrated among the pinealocytes. However, only a few intraparenchymal fluorescent fibers were detected in the proximal third of the gland. With the use of serotonin antiserum serotonin-immunoreactive nerve fibers were clearly restricted to the ventroproximal part of the pineal organ. Although the somata of the pinealocytes showed intense immunoreactivity, their processes were not stained. In one exceptional case, clusters of pinealocytes displaying very intense immunoreactivity were found in an area extending from the distal margin of the ventral portion of the pineal stalk to the proximal portion of the pineal organ proper; these cells were bipolar or multipolar and endowed with well-stained processes. 相似文献
57.
Reproductive structures and phylogenetic significance of extant primitive Angiosperms 总被引:2,自引:0,他引:2
Peter K. Endress 《Plant Systematics and Evolution》1986,152(1-2):1-28
A brief survey is presented on fossil reproductive structures of early Angiosperms from the Lower and mid-Cretaceous and at the same time on the reproductive structures of those extant Angiosperms which resemble most closely these fossils and which seem to be especially primitive also on other grounds: a first group (Degeneriaceae, Himantandraceae, Eupomatiaceae, Austrobaileyaceae) possessing relatively complicated and conspicuous flowers with elaborated inner staminodes, a second group (Chloranthaceae, Trimeniaceae, Amborellaceae) possessing small and relatively simple, inconspicuous flowers with peculiar features in the carpels, and a third group (Winteraceae) possessing flowers with unusual variability in organ number and size. The three groups exhibit a certain diversity in pollination biology, although cantharophily seems to prevail, however different the cantharophily character syndromes may be between the groups. In the extant primitiveMagnoliidae variability occurs on other morphological levels than in the higher advanced Angiosperms. This has to be taken into consideration in evaluations of the systematic relationships of the various groups of theMagnoliidae. Presumably often their relationships are closer than it may appear at first sight. This is also true for the three groups here discussed. 相似文献
58.
Muscarinic Acetylcholine Receptors in Torpedo Electric Organ: Effect of Guanine Nucleotides 总被引:2,自引:2,他引:0
Abstract: The effect of guanine nucleotides on the binding properties of presynaptic muscarinic receptors has been studied in a membrane preparation from the electric organ of Torpedo marmorata by measuring the competitive displacement of the radiolabelled antagonist, [3 H]quinuclidinyl benzilate, by nonradioactive muscarinic ligands. The binding of the antagonists, atropine, scopolamine and pirenzepine was to a single class of sites [slope factors (pseudo Hill coefficients) close to 1] and was unaffected by 0.1 m M GTP. The binding of the N -methylated antagonists, N -methylatropine and N -methyl-scopolamine was more complex (slope factors <1) but also insensitive ( N- methylatropine) to 0.1 m M GTP. Agonist binding was complex and could be resolved into two binding sites with relatively high and low affinities. The proportion of high-affinity sites varied with the nature of the agonist (15–80%). Agonist binding was depressed by 0.1 m M GTP, and the order of sensitivity was oxotremorine-M > carbamoylcholine > muscarine > acetylcholine > arecoline > oxotremorine. The binding of pilocarpine, a partial agonist, was unaffected by GTP. With carbamoylcholine as a test ligand the GTP effect on agonist binding was half-maximal at 12 μM. GDP and guanylylimidodiphosphate produced comparable inhibition of carbamoylcholine binding, but GMP and cyclic GMP were ineffective, as were various adenine nucleotides. Analysis of agonist binding in terms of a two-site model indicates that the predominant effect of guanine nucleotides is to reduce the number of sites of higher affinity. 相似文献
59.
The differentiation of glial cells and glia limitans in organ cultures of chick spinal cord 总被引:1,自引:0,他引:1
Summary Differentiation of glial cells and the glia limitans in organ cultures of chick spinal cord explanted at early neural tube
stages, alone or with adjacent tissues, was studied by electron microscopy. Oligodendrocytes and astrocytes comparable to
those seen in the chicken in vivo were observed, mainly in areas of good neuronal differentiation. A glia limitans with basal
lamina, comparable to that in vivo, was found when spinal cord was bordered by normally adjacent tissues. When it was surrounded
by vitelline membrane only, a characteristic limiting layer of glial processes, but no basal lamina, was seen. Contact with
a filter membrane (Millipore) elicited excessive differentiation of glial filaments and modified cell fine structure; no glia
limitans was formed.
Supported by Grant 5 RO 1 NB 0637 from the United States Public Health Service. 相似文献
60.
A multivariate t probability integral 总被引:2,自引:0,他引:2