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71.
Performance in the flight phase of springboard diving is limited by the amounts of linear and angular momentum generated during the takeoff phase. A planar 8-segment torque-driven simulation model combined with a springboard model was used to investigate optimum takeoff technique for maximising rotation in forward dives from the one metre springboard. Optimisations were run by varying the torque activation parameters to maximise forward rotation potential (angular momentum × flight time) while allowing for movement constraints, anatomical constraints, and execution variability. With a constraint to ensure realistic board clearance and anatomical constraints to prevent joint hyperextension, the optimised simulation produced 24% more rotation potential than a simulation matching a 2½ somersault piked dive. When 2 ms perturbations to the torque onset timings were included for the ankle, knee and hip torques within the optimisation process, the model was only able to produce 87% of the rotation potential achieved in the matching simulation. This implies that a pre-planned technique cannot produce a sufficiently good takeoff and that adjustments must be made during takeoff. When the initial onset timings of the torque generators were unperturbed and 10 ms perturbations were introduced into the torque onset timings in the board recoil phase, the optimisation produced 8% more rotation potential than the matching simulation. The optimised simulation had more hip flexion and less shoulder extension at takeoff than the matching simulation. This study illustrates the difficulty of including movement variability within performance optimisation when the movement duration is sufficiently long to allow feedback corrections. 相似文献
72.
The cyclic lipopeptide fengycin, produced by Bacillus subtilis, exhibits its antimicrobial capabilities by altering the integrity of the cell membrane of plant pathogens. Previous work has correlated fengycin activity with membrane characteristics, such as sterol content. This work focused on the influence of fengycin on supported lipid bilayers containing varying levels of ergosterol. Total internal reflection fluorescence (TIRF) microscopy was used to visualize and distinguish ordered (Lβ/Lo) and disordered (Lα/Ld) domains in the model membranes following exposure to low (50 μg) and high (500 μg) fengycin doses. Application of an initial low dose of fengycin to 0% and 3% ergosterol-containing bilayers resulted in redistribution of Lα/Lβ and Lo/Ld domains, respectively, which the bilayers compensated and corrected for over time. These membranes were unable to tolerate a second 50 μg dose or a single high fengycin dose. The 6% ergosterol bilayers were able to tolerate sequential low doses of fengycin. Exposure of these bilayers to the high fengycin dose caused a decrease in the number of Lo domains, albeit less than that seen in the 0% and 3% ergosterol bilayers. Bilayers containing 12% ergosterol, exhibited the least amount of change after fengycin exposure. These were the only bilayer to exhibit an increase in area taken up by ordered domains. These results suggest fengycin may preferentially act on the Lβ or Lo phase, the area in which ergosterol resides. Bilayers containing low levels of ergosterol appear to be more sensitive to the lipopeptide, suggesting ergosterol plays a role in buffering perturbations caused by fengycin. 相似文献
73.
74.
Yongchang Tang Lei Xu Yupeng Ren Yuxuan Li Feng Yuan Mingbo Cao Yong Zhang Meihai Deng Zhicheng Yao 《International journal of biological sciences》2022,18(1):261
MVI has significant clinical value for treatment selection and prognosis evaluation in hepatocellular carcinoma (HCC). We aimed to construct a model based on MVI-Related Genes (MVIRGs) for risk assessment and prognosis prediction in patients with HCC. This study utilized various statistical analysis methods for prognostic model construction and validation in the Cancer Genome Atlas (TCGA) and International Cancer Genome Consortium (ICGC) cohorts, respectively. In addition, immunohistochemistry and qRT-PCR were used to analyze and identify the value of the model in our cohort. After the analyses, 153 differentially expressed MVIRGs were identified, and three key genes were selected to construct a prognostic model. The high-risk group showed significantly lower overall survival (OS), and this trend was observed in all subgroups: different age groups, genders, stages, and grades. Risk score was a risk factor independent of age, gender, stage, and grade. Moreover, the ICGC cohort validated the prognostic value of the model corresponding to the TCGA. In our cohort, qRT-PCR and immunohistochemistry showed that all three genes had higher expression levels in HCC samples than in normal controls. High expression levels of genes and high-risk scores showed significantly lower recurrence-free survival (RFS) and OS, especially in MVI-positive HCC samples. Therefore, the prognostic model constructed by three MVIRGs can reliably predict the RFS and OS of patients with HCC and is valuable for guiding clinical treatment selection and prognostic assessment of HCC. 相似文献
75.
It has been argued that spatially explicit population models (SEPMs) cannot provide reliable guidance for conservation biology because of the difficulty of obtaining direct estimates for their demographic and dispersal parameters and because of error propagation. We argue that appropriate model calibration procedures can access additional sources of information, compensating the lack of direct parameter estimates. Our objective is to show how model calibration using population-level data can facilitate the construction of SEPMs that produce reliable predictions for conservation even when direct parameter estimates are inadequate. We constructed a spatially explicit and individual-based population model for the dynamics of brown bears (Ursus arctos) after a reintroduction program in Austria. To calibrate the model we developed a procedure that compared the simulated population dynamics with distinct features of the known population dynamics (=patterns). This procedure detected model parameterizations that did not reproduce the known dynamics. Global sensitivity analysis of the uncalibrated model revealed high uncertainty in most model predictions due to large parameter uncertainties (coefficients of variation CV 0.8). However, the calibrated model yielded predictions with considerably reduced uncertainty (CV 0.2). A pattern or a combination of various patterns that embed information on the entire model dynamics can reduce the uncertainty in model predictions, and the application of different patterns with high information content yields the same model predictions. In contrast, a pattern that does not embed information on the entire population dynamics (e.g., bear observations taken from sub-areas of the study area) does not reduce uncertainty in model predictions. Because population-level data for defining (multiple) patterns are often available, our approach could be applied widely. 相似文献
76.
Perturbation of DPPC bilayers by high concentrations of pulmonary surfactant protein SP-B 总被引:3,自引:0,他引:3
Deuterium (2H) NMR has been used to observe perturbation of dipalmitoylphosphatidylcholine (DPPC) bilayers by the pulmonary surfactant protein B (SP-B) at concentrations up to 17% (w/w). Previous 2H NMR studies of DPPC/dipalmitoylphosphatidylglycerol (DPPG) (7:3) bilayers containing up to 11% (w/w) SP-B and DPPC bilayers containing up to 11% (w/w) synthetic SP-B indicated a slight effect on bilayer chain order and a more substantial effect on motions that contribute to decay of quadrupole echoes obtained from bilayers of deuterated DPPC. This is consistent with the perturbation of headgroup-deuterated DPPC reported here for bilayers containing 6 and 9% (w/w) SP-B. For the higher concentrations of SP-B investigated in the present work, 2H NMR spectra of DPPC deuterated in both the headgroup and chain display a prominent narrow component consistent with fast, large amplitude reorientation of some labeled lipid. Similar spectral perturbations have been reported for bilayers in the presence of the antibiotic polypeptide nisin. The observation of large amplitude lipid reorientation at high SP-B concentration could indicate that SP-B can induce regions of high bilayer curvature and thus provides some insight into local interaction of SP-B with DPPC. Such local interactions may be relevant to the formation, in vitro and in vivo, of tubular myelin, a unique structure found in extracellular pulmonary surfactant, and to the delivery of surfactant material to films at the air–water interface.Abbreviations DPPC 1,2-dipalmitoyl-sn-glycero-3-phosphocholine - DPPG 1,2-dipalmitoyl-sn-glycero-3-phosphoglycerol - DPPC-d62 1,2-perdeuterodipalmitoyl-sn-glycero-3-phosphocholine - DPPC-d4 1,2-dipalmitoyl-sn-glycero-3-phospho-(, perdeutero)-choline 相似文献
77.
Murkovic M 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》2004,802(1):3-10
Heterocyclic aromatic amines (HAs) are mutagenic and carcinogenic substances that are formed in significant amounts during heating of meat or fish at temperatures of at least 150 degrees C. To investigate the chemistry lying behind the formation of these harmful substances model systems were established. The first aim was to identify the naturally occurring precursors, namely creatinine, amino acids and carbohydrates. Later these model systems were used to develop strategies for a reduction of the content of the heterocyclic aromatic amines and for the evaluation of the reaction mechanisms that lead to the formation of these substances. All these aspects are discussed in this review. 相似文献
78.
Mingkui?CaoEmail author Stephen D.?Prince Jennifer?Small Scott J.?Goetz 《Ecosystems》2004,7(3):233-242
Spatial and temporal variations in net primary production (NPP) are of great importance to ecological studies, natural resource management, and terrestrial carbon sink estimates. However, most of the existing estimates of interannual variation in NPP at regional and global scales were made at coarse resolutions with climate-driven process models. In this study, we quantified global NPP variation at an 8 km and 10-day resolution from 1981 to 2000 based on satellite observations. The high resolution was achieved using the GLObal Production Efficiency Model (GLO-PEM), which was driven with variables derived almost entirely from satellite remote sensing. The results show that there was an increasing trend toward enhanced terrestrial NPP that was superimposed on high seasonal and interannual variations associated with climate variability and that the increase was occurring in both northern and tropical latitudes. NPP generally decreased in El Niño season and increased in La Niña seasons, but the magnitude and spatial pattern of the response varied widely between individual events. Our estimates also indicate that the increases in NPP during the period were caused mainly by increases in atmospheric carbon dioxide and precipitation. The enhancement of NPP by warming was limited to northern high latitudes (above 50°N); in other regions, the interannual variations in NPP were correlated negatively with temperature and positively with precipitation. 相似文献
79.
We present a model and semiparametric estimation procedures for analysis of survival data with cross-effects (CE) of survival functions. Finite sample properties of the estimators are analyzed by simulation. A goodness-of-fit test for the proportional hazards model against the CE model is proposed. The well known data concerning effects of chemotherapy and radiotherapy on the survival times of gastric cancer patients is analyzed as an example. 相似文献
80.
A stochastic epidemic model is proposed which incorporates heterogeneity in the spread of a disease through a population. In particular, three factors are considered: the spatial location of an individual's home and the household and school class to which the individual belongs. The model is applied to an extremely informative measles data set and the model is compared with nested models, which incorporate some, but not all, of the aforementioned factors. A reversible jump Markov chain Monte Carlo algorithm is then introduced which assists in selecting the most appropriate model to fit the data. 相似文献