Responses of microbial components of the rhizosphere to plant management strategies in a semiarid rangeland

Summary Easily soluble heavy-metal fractions from different soils, a garbage-sewage sludge compost and peat were extracted by EUF. Blanks were determined by extracting distilled water. As the rubber seal of the extraction chamber contained Zn, the obtained Zn values were not reliable. The relative standard deviations of extracted micronutrients were 29.1% for Fe and 20.5% for Mn, Fe, Mn, Zn, Cu, Pb, Cd and Cr were not only found in the filters but also in the extracts.The extraction of CrIII and CrVI solutions showed that CrVI mainly migrated into the anode extract. CrIII was found mainly in the cathode filter and cathode extract, a smaller part however was obviously oxidized to CrVI and migrated into the anode extract. Consequently, CrIII and CrVI in soils could not be distinguished unequivocally by EUF.The amounts of Zn, Cu, Pb and Cd extracted by EUF from various substrates were small compared with the quantities extracted by 2N HCl. The heavy metal contents of the leaves were mostly in the order of those of the EUF extract.Several vineyard soils as well as peat were mixed with increasing quantities of Grünsalz (green salt), a fertilizer consisting mainly of iron sulphate. High amounts of Grünsalz (100–200 g/200 g soil) were necessary to raise soluble Fe in calcareous soils. In peat, however, small Grünsalz additions (1 g/50 g peat) were sufficient. Soluble Mn and Cu increased too when Grünsalz was added to soil or peat. These results give valuable information on how grapevine chlorosis can be reduced by the use of Grünsalz or mixtures of peat and Grünsalz.     

Effects of liming on the extractability of Fe,Mn, Zn and Cu from a peat medium and the growth and micronutrient uptake of highbush blueberry plants

Summary Levels of extractable micronutrients in a peat and the growth and nutrient uptake of young highbush blueberry plants (Vaccinium corymbosum L cv. Blueray) were studied in a greenhouse experiment in response to liming and two rates of addition of Fe, Mn, Zn and Cu.Levels of extractable micronutrients showed different trends with liming depending upon the extractant used and the element being considered. Levels of 0.05M CaCl₂-extractable Fe, Mn and Zn decreased as the pH was raised whilst those of Cu first decreased and then increased again. There was a general decline in 0.1M HCl-extractable Fe, Mn and Cu with increasing pH but levels of Zn were not greatly affected. Levels of 0.005M DTPA extractable Fe, Mn Zn and Cu generally declined but those extractable with 0.04M EDTA were either unaffected or increased as the pH was raised. Levels of CaCl₂-extractable Mn and Zn were the same order of magnitude as those extractable with HCl, DTPA and EDTA. In contrast, the latter reagents extracted considerably more Fe and Cu than did CaCl₂.Dry matter yields of plants were increased as the pH was raised from 3.9 to 4.3 but then decreased markedly as the pH was raised further to 6.7. With increasing pH, concentrations of plant Fe generally increased those of Mn were decreased and those of Zn and Cu were not greatly affected except for a marked decline in plant Cu at pH 6.7.     

Major and trace element concentrations in needles ofPicea abies: levels,distribution functions,correlations and environmental influences

Summary Eighteen major and trace elements (Al, As, Ba, Br, Ca, Cl, Co, Cu, Fe, K, Mg, Mn, Na, Sb, Sc, Sr, V, Zn) have been determined in needles ofPicea abies by neutron activation analysis. Trees from 31 sites with different characteristics were investigated. The sampled area was 140 km² and centered around the city of Winterthur, Switzerland.The effect of washing the needles before analysis was investigated. Washing was found to be essential for a meaningful determination of most elements. The values of the individual elements could be approximated by normal or by lognormal distributions. The width of these distributions varies greatly, being 14% for Cu and a factor of 5 for Mn. Many elements show highly significant positive or negative correlations.Whereas the levels of the major elements as determined here are in the generally accepted range, the values for most of the minor elements show very poor correspondence with published values. For some elements the present data seem to be the only available values. Distinct environmental influences were only manifest for Na, Cl, Br, and these elements show very high values at sites bordering highways.     

In vitro distribution of porphyrin metabolites from 10−3 M delta-aminolevulinic acid in primary neural tissue cultures

The distribution of porphyrin metabolites (uro-7-6-5-copro-3-proto-heme) from 10^–3 M delta-aminolevulinic acid (ALA) in neuronal and glial primary cultures was examined by an isotopic technique. In both cell types, 8–9% of the total production (cell + medium) was found in the cells and more than 90% in the medium. In both the cells and the medium the predominant porphyrin fraction was uro + 7 carboxylic. The heme fraction of the cells was also very high. Through the exclusion of ALA-synthetase activity, the Uroporphyria-like-model of hepatic porphyria was demonstrated.     

Electroconvulsive Shock and Cyclic AMP Signal Transduction: Effects Distal to the Receptor

Chronic electroconvulsive shock (ECS) induced a significant decrease in noradrenaline- and forskolin-stimulated cyclic AMP production in rat cortical slices, whereas a single ECS had a much smaller effect. In a cortical membrane preparation, adenylate cyclase activity in response to stimulation by forskolin, guanosine-5'-(beta,gamma-imido) triphosphate, and Mn2+ ions was significantly increased in membranes derived from rats that had received chronic ECS, but was either unchanged or reduced in membranes from rats that received a single treatment only. The results are interpreted in terms of changes occurring at components of the adenylate cyclase enzyme distal to the receptor.     

Isolation and characterization of cytoplasmic NADP+-malic enzyme from Lupinus luteus leaves

NADP⁺-dependent malic enzyme (L-malate : NADP⁺ oxidoreductase, decarboxylating, EC 1.1.1.40) was extracted from the leaves of yellow lupine. The purification procedure included fractionation with (NH₄)₂SO₄ and Sephadex G-25 chromatography, followed by purification on DEAE-cellulose and Sephadex G-200 columns. The enzyme was purified 122-fold. The enzyme affinity towards L-malate was found to be significantly higher with Mn²⁺ than with Mg²⁺. The Hill coefficient for Mg²⁺ depended on concentration and was 1.6 for the lower and 3.9 for the higher concentrations. The dependence of the enzyme activity on NADP⁺ followed a hyperbolic curve. K_m values and Hill coefficients for NADP⁺ were similar with both Mn²⁺ and Mg²⁺. The enzyme activity was strictly dependent on divalent cations and followed a sigmoidal curve at least for Mg²⁺. The enzyme had 4-fold higher affinity towards Mn²⁺ than towards Mg²⁺, the K_m values being 0.3 and 1.15 m M respectively. Of several tested organic acids, oxalate was the most effective inhibitor followed by oxaloacetate while succinate was the strongest activator.     

Isoenzymes of cuprozinc superoxide dismutase from Pisum sativum

Two cyanide-sensitive and organic solvent-inactivated superoxide dismutase isoenzymes were purified from pea leaves, Pisum sativum, cv Thomas Laxto     

Interaction between interstitial water and sediment in two cores of Lac Léman,Switzerland

The pore fluids of the sediments collected at the deepest point of Lac Léman (Switzerland) are supersaturated with respect to vivianite and siderite. In the presence of sulphide, the iron solubility is controlled entirely by the amorphous iron sulphides. As the iron (II) becomes dominant, the formation of siderite occurs and evidence of this, in the solid phase, can be obtained by the use of M?ssbauer spectroscopy and some sequential chemical extractions. The amount of ‘siderite iron’ decreases from about 10% near the sediment surface to a few percent in the lower levels of the sediment (<10 cm). Evidence for vivianite formation could not be obtained even in the lower layers, despite the precautions taken to avoid oxidation. Although the trace metal behaviour in the solid phase is well correlated with the iron and manganese, availability in the pore fluid is dependent on the adsorption on, or co-precipitation with, finely dispersed colloids, which pass through a 0.45 μg filter. Trace metal concentrations in pore fluid were not directly related to total elemental concentrations in the solid phase, and did not reflect cumulative trends associated with anthropogenic enrichment.     

The effect of regeneration burning upon the nutrient status of soil in two forest types in southern Tasmania

In two forest types in southern Tasmania, eucalypt rainforest (mixed forest) and eucalypt dry sclerophyll forest, surface soils (0–10 cm) from stands that had been clear-felled and burned between 1976 and 1979 were compared with those from uncut, unburned stands. Factors compared were total organic C, N, P, K, Mg, Ca, Zn, Mn; pH; exchangeable Ca, Mg, and K; cation exchange capacity; extractable P; soil phosphate buffering capacity; and N-mineralisation rates. Sampling started in April 1979 and ended in October 1980. Within each forest type, soils from burned coupes had higher mean values for pH, exchangeable cations, percent base saturation, and nitrate-N produced during aerobic incubation, and had lower mean values for exchangeable acidity and ammonium-N produced during aerobic incubation than soils from unburned coupes. In mixed forest only, soils from burned coupes had higher mean values for extractable P and soil phosphate buffering capacity, and lower mean values for total organic C than those of unburned coupes. There were only small differences between burned and unburned soils in cation exchange capacity and ammonium-N produced during anaerobic incubation. For each burned coupe in mixed forest, with increase in time since burning there was a decrease in pH, an increase in exchangeable acidity, and a decrease in rate of production of nitrate: no changes were detected in other factors. It is concluded that, for clay soils developed on dolerite, the nutritional status of soil in both forest types is probably improved by burning. The improvement lasts for more than 4 years in mixed forest and more than two years in dry sclerophyll forest. Only minor leaching of nutrients to below 10 cm in depth is likely to occur in either type.     

Aspects of the Fe and Mn nutrition of rice plants

Summary In three water-culture experiments, the effects of variations in pH, N form, and Si- and P level on the uptake and translocation of Fe and Mn, and on the chlorophyll contents of lowland rice were examined.It was found that Mn uptake increased with increasing pH, that it was not affected by variations in N form (NO₃ or NH₄), and that Si has a suppressive effect on Mn uptake. With increasing pH, the translocation of Fe to the shoots was reduced. This pH effect might be indirect, in that Fe translocation is hampered by excessive Mn uptake induced by high pH. Variations in N form and in Si level did not influence Fe uptake and- translocation.A combination of high P-and high Mn levels in solution proved to reduce the translocation of Fe to the rice shoots. Precipitation of Mn phosphate on the roots is likely to occur at high concentrations of both Mn and P in the root medium.A negative correlation was found between chlorophyll content and Mn content of the leaves. The chlorophyll content was not related to the iron content of the leaves. It is likely that chlorosis of rice leaves in an early growth stage can be caused by several combinations of the following factors: 1. high Mn supply, 2. NO₃ nutrition inducing an increase in solution pH favouring a further increase in Mn uptake, 3. absence of Si which exerts a suppressive effect on Mn uptake, and 4. high P supply. These factors can induce chlorosis, with and without exerting a concomitant influence on the uptake and translocation of Fe.