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101.
Wei Xu 《Inorganica chimica acta》2011,365(1):297-301
Reaction of fresh Mn(OH)2 precipitate and S-carboxymethyl-l-cysteine (H2SCMC) in aqueous solution afforded a novel chiral 3D coordination polymer Mn(H2O)(SCMC) 1, which crystallizes in the acentric polar space group P21 with cell constants a = 5.079(1) Å, b = 9.617(2) Å, c = 8.649(2) Å, β = 94.40(3)°, V = 421.2(1) Å3, Z = 2, and exhibit a SHG effect and ferroelectricity (a remnant polarization Pr = 0.0159 uC cm−2, coercive field Ec = 0.83 kV cm−2, saturation of the spontaneous polarization Ps = 0.234 uC cm−2). To the best of our knowledge, the present compound represents the first example of S-carboxymethyl-l-cysteine coordination polymers that exhibit possible ferroelectric behavior. The structural analysis revealed that the Mn2+ ions in 1 are each coordinated by one N atom and five O atoms of four S-carboxymethyl-l-cysteine ligand bridges four symmetry-related Mn2+ ions to form 3D MOF of 66 topology type with irregular chiral channels extending along [1 0 0]. The temperature-dependent magnetic susceptibilities shows that 1 obeys Curie-Weiss law χm = C/(T − Θ) with C = 4.23 cm3 mol−1 K and Θ = −5.86 K and the best fit gave a weak antiferromagnetic coupling (J = −0.282(5) cm−1) among Mn ions. 相似文献
102.
High initial Mn(II) concentration results in accumulation of a Mn(III) tartrate complex in the growth medium of Phanerochaete chrysosporium. Since Mn(III) is the major oxidant in ligninolysis by manganese peroxidase, the role of accumulated complex should not be neglected when degradation experiments by a crude culture filtrate are performed. To study the Mn(III) complex oxidative potential it was isolated by absorption to polyamide followed by desorption with an alkaline methanol solution. High performance liquid chromatography analysis and atomic absorption spectroscopy confirmed that the isolate was Mn(III) tartrate. Oxidation of 2,2'-azino-bis(3-ethylbenz-thiazoline-6-sulfonate) was used for testing the temperature and pH stability of the isolate that also intensively oxidized 2,6-dimethoxyphenol. In comparison with the non-isolated complex in the culture filtrate, the isolate showed increased temperature and pH stability. The oxidative potential of the isolated Mn(III) tartrate was additionally tested by decolorization of the synthetic dye Indigo carmine. 相似文献
103.
104.
经DEAE纤维素、Sephacryl S-300和Q-Sepharose柱层析分离纯化,从缺失nifE的棕色固氮菌(Azotobactervinelandii Lipmann)突变种(DJ35)的无细胞粗提物中得到△nifE MoFe蛋白(△nifE Av1).SDS凝胶电泳分析表明,△nifE Av1的亚单位种类和分子量分别与棕色固氮菌野生型(OP)MoFe蛋白(Av1)的α和β亚单位相似.当与固氮酶Fe蛋白(Av2)活性互补时,△nifE Av1不具有还原质子的能力,但从OP Av1中抽提的FeMoco却可使其激活.经过量的邻菲啰啉(o-phen)厌氧处理并经Sephadex G-25柱层析分离后,便得到△nifE Av1 .在同时存在Av2和MgATP发生系统的条件下,△nifE Av1 ,而不是△nifE Av1,可为由KMnO4、高柠檬酸铁、Na2S、Na2S2O4和二硫苏糖醇组成的含Mn重组液(RS-Mn)显著激活.但在缺少MgATP或Av2的条件下,RS-Mn则不能激活△nifE Av1 .这就表明,RS-Mn对△nifE Av1 的激活需要o-phen的预先处理及同时存在Av2和MgATP的这二个条件. 相似文献
105.
The complex pathway of tetrapyrrole biosynthesis can be dissected into five sections: the pathways that produce 5-aminolevulinate (the C-4 and the C-5 pathways), the steps that transform ALA to uroporphyrinogen III, which are ubiquitous in the biosynthesis of all tetrapyrroles, and the three branches producing specialized end products. These end products include corrins and siroheme, chlorophylls and hemes and linear tetrapyrroles. These branches have been subjects of recent reviews. This review concentrates on the early steps leading up to uroporphyrinogen III formation which have been investigated intensively in recent years in animals, in plants, and in a wide range of bacteria.Abbreviations ALA
5-aminolevulinic acid
- ALAS
5-aminolevulinic acid synthase
- GR
glutamyl-tRNA reductase
- GSA
glutamate-1-semialdehyde
- GSAT
glutamate-1-semialdehyde aminotransferase
- HMB
hydroxymethylbilane
- PBG
porphobilinogen
- PBGD
porphobilinogen deaminase
- PBGS
porphobilinogen synthase
- URO
uroporphyrin
- URO'gen
uroporphyrinogen
- US
uroporphyrinogen III synthase 相似文献
106.
We examined the Mn(II) oxidation performance of a bacterium, BY86, collected at Yunotaki Falls Hokkaido, Japan. The bacterium showed rapid oxidation of Mn(II), and brown precipitates containing Mn formed within a few days of incubation. The presence of higher oxidation states of Mn than Mn(II) was ascertained by the UV-vis and XANES sutdy. This bacterium did not oxidize As(III) to As(V) in the absence of Mn. In the presence of Mn, however, As(III) was rapidly oxidized to As(V) on the cell surfaces. These findings indicate that BY86 does not have the ability to directly oxidize As(III) to As(V) within a short period of contact, but indirectly oxidizes it by the Mn oxides generated on the cell surfaces. A phylogenetical study disclosed that BY86 was most closely related to Bacillus cereus with an identity of 99.90%. It is expected that our findings in this study will contribute to the study of Mn(II)-oxidizing bacteria, which play an important role in the biogeochemical cycling of Mn as well as other trace elements including As. 相似文献
107.
The role of reactive nitrogen species in secondary spinal cord injury: formation of nitric oxide, peroxynitrite, and nitrated protein 总被引:8,自引:0,他引:8
To determine whether reactive nitrogen species contribute to secondary damage in CNS injury, the time courses of nitric oxide, peroxynitrite, and nitrotyrosine production were measured following impact injury to the rat spinal cord. The concentration of nitric oxide measured by a nitric oxide-selective electrode dramatically increased immediately following injury and then quickly declined. Nitro-L-arginine reduced nitric oxide production. The extracellular concentration of peroxynitrite, measured by perfusing tyrosine through a microdialysis fiber into the cord and quantifying nitrotyrosine in the microdialysates, significantly increased after injury to 3.5 times the basal level, and superoxide dismutase and nitro-L-arginine completely blocked peroxynitrite production. Tyrosine nitration examined immunohistochemically significantly increased at 12 and 24 h postinjury, but not in sham-control sections. Mn(III) tetrakis(4-benzoic acid)-porphyrin (a novel cell-permeable superoxide dismutase mimetic) and nitro-L-arginine significantly reduced the numbers of nitrotyrosine-positive cells. Protein-bound nitrotyrosine was significantly higher in the injured tissue than in the sham-operated controls. These results demonstrate that traumatic injury increases nitric oxide and peroxynitrite production, thereby nitrating tyrosine, including protein-bound tyrosine. Together with our previous report that trauma increases superoxide, our results suggest that reactive nitrogen species cause secondary damage by nitrating protein through the pathway superoxide + nitric oxide peroxynitrite protein nitration. 相似文献
108.
Manisha Patel 《Journal of neurochemistry》1998,71(3):1068-1074
Abstract: The objective of this study was to determine whether free radicals play a pathogenic role in neuronal apoptosis. The ability of Mn(III) tetrakis(benzoic acid) porphyrin (MnTBAP), a superoxide dismutase mimic, to inhibit staurosporine-induced neuronal apoptosis was tested in mixed cerebrocortical cultures. Staurosporine produced concentration-dependent cell death that was markedly inhibited by MnTBAP. Immunocytochemical analyses of cultures for neuron- and astrocyte-specific markers revealed that high concentrations of staurosporine induced the death of both neurons and astrocytes; both cell types were protected by MnTBAP. A less active congener of MnTBAP failed to protect cells against staurosporine-induced apoptosis. MnTBAP also protected cortical cultures against ceramide-induced apoptosis. These results support a role for oxidative stress in neuronal apoptosis. 相似文献
109.
不同光强下高锰对黄瓜光合作用特性的影响 总被引:10,自引:2,他引:10
采用营养液培养的方法,研究了不同光强下高锰对黄瓜植株生长、叶绿素含量、叶绿素荧光参数和光合作用的影响.结果表明,高锰处理抑制了黄瓜植株的生长,与弱光处理相比强光下抑制幅度更加显著.强光下,高锰处理显著降低叶绿素含量,但降低光强却增加其含量.强光下,高锰处理显著降低原初光能转换效率(Fv/Fm)、光合电子传递量子效率(ΦPSII)和光化学猝灭系数(qP);弱光下,高锰处理对Fv/Fm和qP无显著影响.高锰处理使净光合速率(Pn)和气孔导度(Gs)下降.尤其是在强光下下降幅度更大.高锰处理使细胞间CO2浓度(Ci)在强光下升高,而在弱光下则下降.与Ci相反,高锰处理使气孔限制值(Ls)在强光下下降,而在弱光下上升.因此,强光下高锰胁迫使净光合速率下降可能是由非气孔限制引起的,而弱光下高锰处理使净光合速率下降可能是由气孔因子限制引起的. 相似文献
110.
《Saudi Journal of Biological Sciences》2022,29(4):2238-2246
Spinach (Spinacia oleracea L.) is considered a nitrogen (N) intensive plant with high nitrate (NO3?) accumulation in its leaves. The current study via a two-year field trial introduced an approach by combining N fertilization from different sources (e.g., ammonium nitrate; 33.5 % N, and urea; 48 % N) at different rates (180, and 360 kg N ha?1) with the foliar spraying of molybdenum (Mo) as sodium molybdate, and/or manganese (Mn) as manganese sulphate at rates of 50 and 100 mgL?1 of each or with a mixture of Mo and Mn at rates of 50 and 50 mg L?1, respectively on growth, chemical constituents, and NO3? accumulation in spinach leaves. Our findings revealed that the highest rate of N fertilization (360 kg N ha?1) significantly increased most of the measured parameters e.g., plant length, fresh and dry weight plant?1, number of leaves plant?1, leaf area plant?1, leaf pigments (chlorophyll a, b and carotenoids), nutrients (N, P, K, Fe, Mn, Zn), total soluble carbohydrates, protein content, net assimilation rate, and NO3? accumulation, but decreased leaf area ratio and relative growth rate. Moreover, plants received urea-N fertilizer gave the highest values of all previous attributes when compared with ammonium nitrate –N fertilizers, and the lowest values of NO3? accumulation. The co-fertilization of N-Mo-Mn gave the highest values in all studied attributes and the lowest NO3? accumulation. The best treatment was recorded under the treatment of 360 kg N-urea ha?1 in parallel with the combined foliar application of Mo and Mn (50 + 50 mg L?1). Our findings proposed that the co-fertilization of N-Mo-Mn could enhance spinach yield and its quality, while reducing NO3? accumulation in leaves, resulting agronomical, environmental and economic benefits. 相似文献