全文获取类型
收费全文 | 24638篇 |
免费 | 1596篇 |
国内免费 | 1818篇 |
出版年
2024年 | 69篇 |
2023年 | 288篇 |
2022年 | 434篇 |
2021年 | 574篇 |
2020年 | 680篇 |
2019年 | 884篇 |
2018年 | 730篇 |
2017年 | 625篇 |
2016年 | 624篇 |
2015年 | 705篇 |
2014年 | 1114篇 |
2013年 | 1406篇 |
2012年 | 809篇 |
2011年 | 1062篇 |
2010年 | 835篇 |
2009年 | 1105篇 |
2008年 | 1134篇 |
2007年 | 1255篇 |
2006年 | 1127篇 |
2005年 | 1058篇 |
2004年 | 904篇 |
2003年 | 852篇 |
2002年 | 821篇 |
2001年 | 697篇 |
2000年 | 661篇 |
1999年 | 628篇 |
1998年 | 606篇 |
1997年 | 532篇 |
1996年 | 583篇 |
1995年 | 495篇 |
1994年 | 461篇 |
1993年 | 500篇 |
1992年 | 427篇 |
1991年 | 420篇 |
1990年 | 345篇 |
1989年 | 288篇 |
1988年 | 286篇 |
1987年 | 248篇 |
1986年 | 211篇 |
1985年 | 260篇 |
1984年 | 277篇 |
1983年 | 159篇 |
1982年 | 201篇 |
1981年 | 166篇 |
1980年 | 138篇 |
1979年 | 98篇 |
1978年 | 92篇 |
1977年 | 50篇 |
1976年 | 45篇 |
1975年 | 24篇 |
排序方式: 共有10000条查询结果,搜索用时 19 毫秒
91.
Summary Cucumber seedlings were grown in a Portsmouth soil-sand system to study how varying soil clay and organic matter content might
modify cucumber seedling response to ferulic acid, a reported allelopathic agent. Leaf area expansion of cucumber seedlings,
soil respiration, and soil solution concentrations of ferulic acid were monitored. Leaf area, mean absolute rates of leaf
expansion, and shoot dry weight of cucumber seedlings were significantly reduced by ferulic acid concentrations ranging from
10 to 70 μg/g dry soil. Ferulic acid was applied every other day, since it rapidly disappeared from soil solution as a result
of retention by soil particles, utilization by microbes and/or uptake by roots. The amount of ferulic acid retained (i.e., adsorbed, polymerized,etc.) by soil particles appeared to be secondary to microbial utilization and/or uptake by roots. Varying clay (5.3 to 9.8 g/cup)
and organic matter (2.0 to 0.04g/cup) contents of the soil appeared to have little impact on the disappearance of ferulic
acid from soil solution under “ideal” growth conditions for cucumber seedlings unless larger amounts of ferulic acid were
added to the soil; in this case 200 μg/g. The addition of ferulic acid to the soil materials substantially increased the activity
of the soil microbes. This latter conclusion is based on recovery of ferulic acid from soil solution and soil respiration
measurements.
Paper No. 10347 of the Journal Series of the North Carolina Agricultural Research Service, Raleigh, N C 27695-7601. The use
of trade names in this publication does not imply endorsement by the North Carolina Agricultural Research Service of the product
named, nor criticism of similar ones not mentioned. 相似文献
92.
Relation between electrokinetic potentials and growth in callus cultures ofTrigonella foenum-graecum
Callus cultures ofTrigonella foenum-graecum were initiated from radicle or cotyledon portions of seedlings and young leaves and maintained on modified 1-B5 medium. The
callus mass was disaggregated by mechanical agitation and the discrete cells thus obtained were used to measure their electrokinetic
potential. Studies pertaining to the effects of ageing on electrokinetic potential and growth index revealed a relationship
between these two parameters. Thus, the rate of change of electrokinotie potential with age could be employed as a parameter
to study the growth kinetics of cells in callus cultures. 相似文献
93.
94.
95.
The influence of diet on the growth of Stenonema vicarium (Walker) (Ephemeroptera: Heptageniidae) 总被引:1,自引:1,他引:0
Laboratory studies compared the growth rate of Stenonema vicarium (Walker) nymphs on diets of detritus and natural stream periphyton. In three consecutive runs of the experiment, growth rates
were consistently higher on periphyton (mean growth rate = 2.1% wet wt. d−1) than detritus (mean = 1.8% wet wt. d−1). The starting date of each run also significantly influenced growth rates. In each treatment growth rates generally decreased
over the course of the 3 runs, and ca. one-half of the nymphs in the last run did not molt or grow. It appeared that growth
of S. vicarium may be partially controlled by seasonal factors. 相似文献
96.
Summary The physical localization of sequences homologous to three cloned genes was determined by in situ hybridization to metaphase chromosomes. Previous work had assigned the skeletal myosin heavy chain gene cluster (Myh), the functional locus for the cellular tumor antigen p53 (Trp53-1), and the cellular homologue of the viral erb-B oncogene (Erbb) toMus musculus chromosome 11 (MMU11). Our results provide regional assignments ofMyh andTrp53-1 to chromosome bands B2C, and ofErbb to bands A1A4. Taken together with in situ mapping of three other loci on MMU 11 (Hox-2 homeobox-containing gene cluster, theSparc protein, and theColla-1 collagen gene), which have been reported elsewhere, these data allowed us to construct a physical map of MMU11 and to compare it with the linkage map of this chromosome. The map positions of the homologous genes on human chromosomes suggest evolutionary relationships of distinct regions of MMU11 with six different human chromosome arms: 1p, 5q, 7p, 16p, 17p, and 17q. The delineation of conserved chromosome regions has important implications for the understanding of karyotype evolution in mammalian species and for the development of animal models of human genetic diseases. 相似文献
97.
Magdalena Torres M. France Bader Dominique Aunis M. Teresa Miras-Portugal 《Journal of neurochemistry》1987,48(1):233-235
Chromaffin cells both recently isolated or in culture present a high-affinity adenosine transporter with a Km value of 1 microM. When cells were exposed to nerve growth factor (NGF; 10 ng/ml), the adenosine transporter affinity decreased to 3 microM. This value was maintained from 3 days after plating to the end of the culture period. A change in the transport capacity was observed, with a significant increase (approximately 200-260%) in NGF-cultured cells throughout the period studied. 相似文献
98.
Platelet-derived growth factor (PDGF) increases the mitogenic activity of epidermal growth factor (EGF) in several cells lines, including BALB/C-3T3. PDGF-treated BALB/C-3T3 cells manifest a reduced capacity to bind 125I-labeled EGF due to a loss of high affinity EGF receptors. Cholera toxin potentiates the ability of PDGF to both decrease EGF binding and initiate mitogenesis. Whether PDGF increases EGF sensitivity via its effects on EGF receptors is not known and requires a more complete understanding of the mechanism by which PDGF decreases EGF binding. 12-O-tetradecanoylphorbol 13-acetate (TPA) also reduces EGF binding in BALB/C-3T3 and other cells, presumably by activating protein kinase C and, consequently, inducing the phosphorylation of EGF receptors at threonine-654. PDGF indirectly activates protein kinase C, and EGF receptors in PDGF-treated WI-38 cells are phosphorylated at threonine-654. Thus, the effects of PDGF on EGF binding may also be mediated by protein kinase C. We investigated this hypothesis by comparing the actions of PDGF and TPA on EGF binding in density-arrested BALB/C-3T3 cells. Both PDGF and TPA caused a rapid, transient, cycloheximide-independent loss of 125I-EGF binding capacity. The actions of both agents were potentiated by cholera toxin. However, whereas TPA allowed EGF binding to recover, PDGF induced a secondary and cycloheximide-dependent loss of binding capacity. Most importantly, PDGF effectively reduced binding in cells refractory to TPA and devoid of detectable protein kinase C activity. These findings indicate that PDGF decreases EGF binding by a mechanism that involves protein synthesis and is distinct from that of TPA. 相似文献
99.
Restriction fragment length polymorphisms in dairy and beef cattle at the growth hormone and prolactin loci 总被引:2,自引:0,他引:2
Two bovine populations, a Holstein-Friesian dairy stock and a synthetic (Baladi X Hereford X Simmental X Charolais) beef stock, were screened for restriction fragment length polymorphisms (RFLPs) at the growth hormone and prolactin genes. Most RFLPs at the growth hormone gene are apparently the consequence of an insertion/deletion event which was localized to a region downstream of the structural gene. The restriction map for the genomic region including the growth hormone gene was extended. Two HindIII RFLPs at the growth hormone locus, as well as several RFLPs at the prolactin gene, seemed to be the consequence of a series of point mutations. The results are discussed in terms of the possibility that minor genomic variability underlies quantitative genetic variation. 相似文献
100.
S. L. Macaulay Julie D. Newman J. D. Mc Armstrong J. Bornstein 《Molecular and cellular biochemistry》1987,74(1):95-101
The N-terminal part sequences of pituitary growth hormone, N-acetyl-hGH 7–13 and hGH 6–13, promoted conversion of glycogen synthase b to glycogen synthase a in skeletal muscle and adipose tissue when injected intravenously. The peptides also caused conversion of phosphorylase a to phosphorylase b in liver and adipose tissue, but not in muscle, where the peptides antagonised activation of phosphorylase. Synthase phosphatase activity in muscle and phosphorylase phosphatase activity in liver increased after injection of peptide, with time courses of change similar to those seen for muscle synthase and liver phosphorylase activities. Injection of peptide also decreased both the cyclic AMP dependent and independent synthase kinase activities in muscle. These results show that the insulin-like activities of these peptides on glycogen synthase and phosphorylase involve both increases in protein phosphatase activities and inhibition of protein kinase activities. These results are discussed in relation to the insulin-like activities of growth hormone. 相似文献