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961.
Characterization of the microbial community of lotic organic aggregates ('river snow') in the Elbe River of Germany by cultivation and molecular methods 总被引:10,自引:0,他引:10
Aerobic and anaerobic cultivation techniques, 16S rDNA-based phylogeny, and fluorescent in situ hybridization were used to describe the phylogenetic diversity and physiological versatility of lotic microbial aggregates ('river snow') obtained from the river Elbe. In the course of the year the 'river snow' community changed. It was characterized by a great bacterial diversity in spring, the predominant occurrence of algae in summer and reduction of the total bacterial cell count in autumn and winter. In all 'river snow' samples, more than 70% of the bacteria counted with the general DNA stain DAPI also hybridized with the Bacteria-specific probe EUB338. In situ analysis of the bacterial 'river snow' community with a comprehensive suite of specific rRNA-targeted probes revealed population dynamics to be governed by seasonal factors. During all seasons, beta-Proteobacteria constituted the numerically most important bacterial group forming up to 54% of the total cell counts. In contrast to this, the relative abundance of other major bacterial lineages ranged from 2% for the order Planctomycetales to 36% for Cytophaga-Flavobacteria. Cultivation of 'river snow' under aerobic and anaerobic conditions with a variety of different media resulted in the isolation of 40 new bacterial strains. Phenotypic and phylogenetic analyses revealed these new strains to be mostly unknown organisms affiliated to different bacterial phyla. Application of newly developed specific oligonucleotide probes proved the cultivated bacteria, including clostridia and the numerically abundant beta-Proteobacteria, as relevant in situ members of the 'river snow' community. 相似文献
962.
Effects of habitat size and quality on equilibrium density and extinction time of Sorex araneus populations 总被引:1,自引:0,他引:1
1. The effects of changes in habitat size and quality on the expected population density and the expected time to extinction of Sorex araneus are studied by means of mathematical models that incorporate demographic stochasticity.
2. Habitat size is characterized by the number of territories, while habitat quality is represented by the expected number of offspring produced during the lifetime of an individual.
3. The expected population density of S. araneus is shown to be mainly influenced by the habitat size. The expected time to extinction of S. araneus populations due to demographic stochasticity, on the other hand, is much more affected by the habitat quality.
4. In a more general setting we demonstrate that, irrespective of the actual species under consideration, the likelihood of extinction as a consequence of demographic stochasticity is more effectively countered by increasing the reproductive success and survival of individuals then by increasing total population size. 相似文献
2. Habitat size is characterized by the number of territories, while habitat quality is represented by the expected number of offspring produced during the lifetime of an individual.
3. The expected population density of S. araneus is shown to be mainly influenced by the habitat size. The expected time to extinction of S. araneus populations due to demographic stochasticity, on the other hand, is much more affected by the habitat quality.
4. In a more general setting we demonstrate that, irrespective of the actual species under consideration, the likelihood of extinction as a consequence of demographic stochasticity is more effectively countered by increasing the reproductive success and survival of individuals then by increasing total population size. 相似文献
963.
964.
To understand the ecological roles of epiphytic bryophytes in the carbon (C) and nitrogen (N) cycles of a tropical montane forest, we used samples in enclosures to estimate rates of growth, net production, and N accumulation by shoots in the canopy, and litterbags, to estimate rates of decomposition and N dynamics of epiphytic bryophyte litter in the canopy and on the forest floor in Monteverde, Costa Rica. Growth of epiphytic bryophytes was estimated at 30.0–49.9 percent/yr, net production at 122–203 g/m2/yr, and N accumulation at 1.8–3.0 g N/m2/yr. Cumulative mass loss from litterbags after one and two years in the canopy was 17 ± 2 and 19 ± 2 percent (mean ± 1 SE) of initial sample mass, respectively, and mass loss from litter and green shoots in litterbags after one year on the forest floor was 29 ± 2 and 45 ± 3 percent, respectively. Approximately 30 percent of the initial N mass was released rapidly from litter in both locations. Nitrogen loss from green shoots on the forest floor was greater; about 47 percent of the initial N mass was lost within the first three months. There was no evidence for net N immobilization by litter or green shoots, but the remaining N in litter was apparently recalcitrant. Annual net accumulation of C and N by epiphytic bryophytes was estimated at 37–64 g C/m2/yr and 0.8–1.3 g N/m2/yr, respectively. Previous research at this site indicated that epiphytic bryophytes retain inorganic N from atmospheric deposition to the canopy. Therefore, they play a major role in transforming N from mobile to highly recalcitrant forms in this ecosystem. 相似文献
965.
Clb5-associated Kinase Activity is Required Early in the Spindle Pathway for Correct Preanaphase Nuclear Positioning in Saccharomyces cerevisiae 总被引:1,自引:0,他引:1
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In Saccharomyces cerevisiae, a single cyclin-dependent kinase, Cdc28, regulates both G1/S and G2/M phase transitions by associating with stage-specific cyclins. During progression through S phase and G2/M, Cdc28 is activated by the B-type cyclins Clb1–6. Because of functional redundancy, specific roles for individual Clbs have been difficult to assign. To help genetically define such roles, strains carrying a cdc28ts allele, combined with single CLB deletions were studied. We assumed that by limiting the activity of the kinase, these strains would be rendered more sensitive to loss of individual Clbs. 相似文献
966.
967.
Carbon and nutrient dynamics in relation to growth rhythm in the giant bamboo Phyllostachys pubescens 总被引:2,自引:0,他引:2
The carbon and nutrient dynamics in relation to growth rhythm in the giant bamboo Phyllostachys pubescens on Mount Jinyun, Chongqing, China, was studied during 1993–1996. Concentrations of TNC (total non-structural carbohydrates), N, P, and K all showed the same distribution pattern among organs: leaves > branches rhizomes stems roots. The rapid spring growth of new shoots noticeably reduced the concentration of TNC in the rhizomes, in which a large amount of carbohydrates was stored. The N concentration of the rhizomes did not decrease, however. Nutrient concentration of new (1st-year) leaves was significantly higher than that of old (2nd-year) leaves. Although the density of adult shoots was almost the same during 1994–1996, the low ratio of the number of adult shoots with new to that with old leaves from June 1994 to April 1995 resulted in a low TNC concentration in the rhizomes in early spring (April) 1995. This led to a low production of new shoots in the spring of 1995, their number being only ca. 10% of that in 1994 and 1996. Before old leaves were shed, a large amount of nutrients was remobilized and translocated to other plant parts to support further growth. Fertilization with NPK significantly increased the concentrations of N and P in leaves and subsequently increased the number of emerging new shoots. 相似文献
968.
969.
Many species of Eucalyptus in Australia provide copious amounts of nectar during their reproductive seasons. The nectar is used by many animal species but especially by birds, insects and some bats, which act as pollinators. One of the major features of eucalypt flowering in southern Australia is the patchy, asynchronous flowering of different species, which appears to drive mass nomadism of nectarivorous birds among regions and among habitats. Here we explore whether flowering asynchrony or climate is primarily responsible for the influxes and effluxes of vast numbers of nectarivorous birds in central Victoria, Australia. By using a structured sampling program, we show that winter flowering by red ironbark Eucalyptus tricarpa is the most likely agent controlling avian-nectarivore dynamics rather than climatic differences among regions. Densities and species richness of nectarivores, and numbers of nectarivory events, are all closely related to measures of flowering intensity. However, nonnectarivores, such as insectivores and granivores, show no relationships with either habitat or region. We discuss how dependence on a patchily distributed but highly rewarding resource such as nectar influences population densities and community structure in birds. 相似文献
970.
Eric F. Salamanca Nobuhiro Kaneko Shigeo Katagiri Yasuhide Nagayama 《Ecological Research》1998,13(2):199-210
The litter mass loss, concentration and mass of some major nutrient elements, degradation of lignin and cellulose in decomposing Quercus serrata Murray leaf litter were monitored for 3 years using the litterbag method. The mobility of elements during the course of the study was in the order of: K > P > C > Mg > Ca > N. Three patterns of nutrient dynamics were observed: (i) concentration increased while mass decreased (N, Mg and Ca); (ii) concentration and nutrient mass decreased (K and C); and (iii) both concentration and mass had fluctuated (P). The C to element ratio tended to increase as the element was released, and decreased as the element was retained. Nitrogen mobility in relation to carbon was characterized by three phases: (i) initial release; (ii) accumulation and (iii) final release. The decay rate (k) calculated from 0–6 months period was overestimated for an average annual rate while those of 0–36 months fit the negative single exponential model (Adj. r2 = 0.99) better than shorter periods. For lignin, the concentration had increased then decreased but tended to stabilize after 1 year while the lignin mass had continuously decreased throughout the study period. During the first 9 months, both the concentrations and mass of cellulose had fluctuated but declined thereafter. The amounts of N had initially increased but declined after 1 year; P had fluctuated while K, Ca, Mg and C had decreased throughout the study. N and C/N ratio exerted strong influence on mass loss during the first24 months but the influence of lignin emerged after 24 months. 相似文献