排序方式: 共有80条查询结果,搜索用时 15 毫秒
31.
微生物基因组注释系统MGAP 总被引:6,自引:0,他引:6
利用生物信息学方法和工具开发了微生物基因组注释系统(Microbial genome annotation package, MGAP),并用于蓝细菌PCC7002的基因组注释。该系统由基因组注释系统和基于Web的用户接口程序两部分组成。基因组注释系统整合多个基因识别、功能预测和序列分析软件;以及蛋白质序列数据库、蛋白质资源信息系统和直系同源蛋白质家族数据库等。用户接口程序包括基因组环状图展示、基因和开放读码框在染色体上的分布图,以及注释信息检索工具。该系统基于PC微机和Linux操作系统,用MySQL作数据库管理系统、用Apache作Web服务器程序,用Perl脚本语言编写应用程序接口,上述软件均可免费获得。 相似文献
32.
Coral tumors are atypical skeletal forms found on coral reefs worldwide. Here we present an analysis of the microbial communities associated with skeletal tumors on the coral Porites compressa. Microbial growth rates on both healthy and tumorous P. compressa were decoupled from the surrounding water column. Microbial communities associated with tumorous colonies had a significantly faster growth rate than those associated with healthy P. compressa. The microbial community associated with the tumors contained more culturable Vibrio spp. and could utilize more carbon sources than the microbes associated with healthy colonies. Presence of tumors affected the composition and dynamics of the microbial population associated with the entire colony. 相似文献
33.
超高压在工业微生物诱变选育中的应用初探 总被引:9,自引:0,他引:9
超高压对微生物有多方面的影响,它不仅可使微生物细胞组分、体积形态发生变化,还可使微生物的基因表达和核酸结构及其生物学功能发生改变。超高压的这些生物学效应,使其不仅可以应用到食品杀菌、保藏及某些加工过程,而且在微生物菌种诱变方面具有很大的应用潜力。 相似文献
34.
The occurrence of yeasts on ripe fruits and frozen pulps of pitanga (Eugenia uniflora L), mangaba (Hancornia speciosa Gom.), umbu (Spondias tuberosa Avr. Cam.), and acerola (Malpighia glaba L) was verified. The incidence of proteolytic, pectinolytic, and mycocinogenic yeasts on these communities was also determined. A total of 480 colonies was isolated and grouped in 405 different strains. These corresponded to 42 ascomycetous and 28 basidiomycetous species. Candida sorbosivorans, Pseudozyma antarctica, C. spandovensis-like, C. spandovensis, Kloeckera apis, C. parapsilosis, Rhodotorula graminis, Kluyveromyces marxianus, Cryptococcus laurentii, Metchnikowia sp (isolated only from pitanga ripe fruits), Issatchenkia occidentalis and C. krusei (isolated only from mangaba frozen pulps), were the most frequent species. The yeast communities from pitanga ripe fruits exhibited the highest frequency of species, followed by communities from acerola ripe fruits and mangaba frozen pulps. Yeast communities from frozen pulp and ripe fruits of umbu had the lowest number of species. Except the yeasts from pitanga, yeast communities from frozen pulp exhibited higher number of yeasts than ripe fruit communities. Mycocinogenic yeasts were found in all of the substrates studied except in communities from umbu ripe fruits and pitanga frozen pulps. Most of the yeasts found to produce mycocins were basidiomycetes and included P. antarctica, Cryptococcus albidus, C. bhutanensis-like, R. graminis and R. mucilaginosa-like from pitanga ripe fruits as well as black yeasts from pitanga and acerola ripe fruits. The umbu frozen pulps community had the highest frequency of proteolytic species. Yeasts able to hydrolyse casein at pH 5.0 represented 38.5% of the species isolated. Thirty-seven percent of yeast isolates were able to hydrolyse casein at pH 7.0. Pectinolytic yeasts were found in all of the communities studied, excepted for those of umbu frozen pulps. The highest frequency of pectinolytic activity was found in mangaba frozen pulp communities. Around 30% of all isolates produced pectinases. The ability to split arbutin was observed in all communities ranging from 8% in yeasts from pitanga frozen pulps to 40.6% in acerola ripe fruit communities. Among 432 species tested, 125 were active for beta-glucosidase production, and Kloeckera apis, P. antarctica, C. sorbosivorans, and C. spandovensis-like were the most active species. 相似文献
35.
通过测定珠江口海域典型腹足类动物习见蛙螺(Bursa rana)和棒锥螺(Turritellabacillum)各组织器官中重金属(Cu、Zn、Pb、Cd和As)的含量,研究了腹足类动物重金属的组织分布及累积模式.结果表明:肠与肝脏重金属累积模式差异较大,腮在两种螺累积重金属的过程中可能扮演着重要角色,性腺是重金属在螺体的重要累积器官;人体通过食用习见蛙螺和棒锥螺肌肉摄入的重金属虽未超过国际食品法典委员会提出的最大容许摄入量,但重金属联合毒性的潜在危害值得引起高度关注. 相似文献
36.
37.
一类微生物食物链模型的持续生存 总被引:1,自引:0,他引:1
讨论了一类n维微生物食物链模型的持续生存问题,假设模型中微生物的增长率具有Monod内在代谢形式.证明了模型有一个解平面.并且模型所有的解以此解平面为ω极限集,因此所有的解是有界的;并证明了满足一定条件时,n维食物链模型是持久的. 相似文献
38.
为了了解深圳海域养殖牡蛎体内的卫生质量状况,我们于2002年1月~2004年2月对该海域养殖牡蛎体内的污染物水平进行了检测,结果显示六六六(BHC)为ND(未检出)~0.100mg?Kg-1湿重,DDT为0.004~0.255mg·Kg-1湿重,细菌总数为ND~9.3104Ind·g-1,大肠菌群为ND~5.6104Ind·(100g)-1,PSP为ND~34礸·(100g)-1,副溶血性弧菌沙井和后海的近江牡蛎检测结果为阳性,其余站位未检出。参照GB18406.4-2001《农产品安全质量无公害水产品安全要求》,深圳海域养殖牡蛎的六六六、DDT、细菌总数和PSP完全符合要求,沙井和后海的近江牡蛎副溶血性弧菌超标,所有站位的大肠菌群均存在超标。综合重金属的检测结果发现,所有站位的牡蛎均有多个污染物超标,卫生质量欠佳,珠江口和深圳湾的近江牡蛎较大亚湾和大鹏湾的长牡蛎和褶牡蛎卫生质量更差。 相似文献
39.
40.
微生物细胞表面工程是近年来发展起来的,它利用细胞表面展示技术使外源蛋白固定化于细胞表面,从而生产微生物细胞表面蛋白。微生物细胞表面工程可用于细胞催化剂、细胞吸附剂、活疫苗、生物传感器的开发等。微生物细胞表面工程具有广阔的应用前景,但是国内对这一领域的研究刚起步。在介绍细胞表面工程的基础上,对微生物细胞表面工程技术进展进行了综述,展望了对该技术的发展。 相似文献