LncRNA-NNT-AS1 contributes to the progression of glioma by miR-582-5p/EZH2 axis

Cytotechnology - Increasing studies have shown that long non-coding RNAs (lncRNAs) had crucial regulatory roles in many diseases. Nevertheless, the biological relevance and mechanisms of the...     

Énuloses dérivés di-O-isopropylidènes du d-glucose,d-galactose et d-fructose

The production of modified amyloses often involves the p-toluenesulfonylation of the primary hydroxyl group of the d-glucopyranosyl residues, but it was not known whether this esterification is random when ～20% of the d-glucopyranosyl residues have been substituted. The reaction of p-toluenesulfonyl chloride with amylose in pyridine, in C-methylpyridines, in aqueous 2m KOH, and in dimethyl sulfoxide-pyridine yielded unsatisfactory products. However, p-toluenesulfonylation of 2,3-di-O-acetylamylose in pyridine yielded products that were converted into 2,3-di-O-acetyl-6-deoxy-6-iodoamylose, and thence into 6-deoxyamylose, a suitable substrate for the amylase to be used in the determination of random substitution. The hydrolysis products formed by the action of the crystalline, liquefying amylase of Bacillus subtilis were separated and analyzed. When a summation of the minimum number of separated, modified D-glucopyranosyl residues was compared to a computer-based calculation of the clustering expected, the results showed random esterification.     

Synthése du 6-O-β-D-Galactofuranosyl-D-galactopyranose

6-O-β-D-Galactofuranosyl-D-galactose was obtained in crystalline form by condensation of 3,5,6-tri-O-acetyl-1,2-O-(1-methoxyethylidene)-α-D-galactofuranose with benzyl 2,3,4-tri-O-benzyl-α-D-galactopyranoside, followed by O-deacetylation and catalytic hydrogenation. This compound is identical with that isolated from the cell wall of Mycobacterium tuberculosis by partial acid hydrolysis.