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41.
Koshi Saito Hamako Obata-Sasamoto Shin-Ichi Hatanaka Hiroshi Noguchi Ushio Sankawa Atsushi Komamine 《Phytochemistry》1982,21(2):474-476
Isolation and identification of l-3-carboxy-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline and l-1-methyl-3-carboxy-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline from seeds and callus of S. hassjoo are described. Administration of [β-14C]-labelled DOPA to a callus culture of this legume resulted in the incorporation of radioactivity into l-3-carboxy-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline, l-1-methyl-3-carboxy-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline and stizolobic acid, which was confirmed by constant specific radioactivity after co-crystallization with authentic samples of each compound. 相似文献
42.
Ikram-ul-Haq Zhao Chang-Xing Zahid Mukhtar Cheruth Abdul Jaleel Mohamed Mahgoub Azooz 《Journal of plant physiology》2009,166(14):1568-1575
This experiment assessed the effect of partial physical desiccation on plant regeneration efficiency in scutellum-derived embryogenic calluses of rice (Oryza sativa L.) variety Super basmati. A number of callusing cultures were developed, and efficient callus induction was observed on MS (Murashige and Skoog) basal medium supplemented with 2.0 mg/L 2,4-dichlorophenoxy acetic acid. The calluses were proliferated on the same medium for 3 weeks and then shifted to dehydration desiccation treatment for 72 h. The desiccated calluses were cultured on different media for somatic embryogenesis and plant regeneration. A medium with 2.0 mg/L α-napthaleneacetic acid, 10.0 mg/L abscisic acid , 2.0 mg/L kinetin was best for somatic embryogenesis only, but not for further plant development. After 10 d, differentiated calluses were sub-cultured on medium with various concentrations and types of carbohydrates (carbon source) in 1MS2j medium. A large number of plantlets (14.51±2.81 and 8.56±2.90 plants/callus) were regenerated via chemical desiccation, on MS with 3% maltose+3% sorbitol and 6% sucrose, respectively. Under dehydration on only simple MS (3% sucrose), 11.23±3.22 plants/callus were developed. Under conditions of dehydration and chemical desiccation, plant regeneration rates were higher than the calluses cultured on simple MS medium in the presence of plant growth regulator. After somatic embryogenesis, >25% plants were sterile. The protocol used here may allow maximum regeneration of normal and fertile plantlets of super basmati rice within 3 months. 相似文献
43.
Studies were performed to define tissue culture techniques and culture conditions for morphogenesis, callus culture and plantlet culture of sweet orange (Citrus sinensis (L.) Osb.), citron (C. medica L.) and lime (C. aurantifolia) (Christm. Swing). The optimal concentrations of NAA to induce root formation on stem segments were 10 mg l-1 for sweet orange and lime, and 3 mg l-1 for citron. The optimal BA concentration for shoot and bud proliferation was 3 mg l-1 for sweet orange and citron, and 1 mg l-1 for lime. Callus initiation was accomplished in a culture medium containing 10 mg l-1 NAA and 0.25 mg l-1 BA. Callus was maintained by periodical subculture into the same medium supplemented with 10% (v:v) organge juice. In vitro plantlets of the three species were obtained by rooting of shoots developed from bud cultures, and of citron and lime by development of shoots from root cultures. The plants were successfully established on soil. 相似文献
44.
The effect of Decis (deltamethrin as active ingredient) on callus tissue of Solanum tuberosum L. cv. Désirée was studied. Decis is an agrochemical currently used on field grown potato plants to control the Colorado beetle, a potato pest. Deltamethrin added to the culture medium interferes with the behaviour of callus tissue. After 5 h of culture, the level of total proteins was higher in treated tissue than in the control, and SDS-PAGE showed that deltamethrin promoted the increase of some soluble proteins. After 24 h and 14 days of culture, the level of total proteins became similar in both treated and control material. This similarity between control and treated tissues, after 14 days of culture, occurred as a result of the treatment with deltamethrin which caused a decrease of soluble proteins, but an increase in insoluble proteins whereas the opposite was observed for control callus. SDS-PAGE of both soluble and insoluble proteins showed that only quantitative dissimilarities occurred. This longer treatment also increased the chlorophyll content. Ultrastructural study of the cells revealed that tissue callus cultured for 14 days in the presence of deltamethrin had plastids containing a more developed membranous system, with a higher number of grana and with more compartments than in control cells. Deltamethrin also promoted the abundance of vesicles associated with dictyosomes. This response of the callus tissue to Decis added to the callus medium parallels the behaviour of potato plants treated with this agrochemical under field conditions (Fidalgo, Santos & Salema, 1993). 相似文献
45.
J.?B.?Kim J.?E.?M.?Bergervoet C.?J.?J.?M.?RaemakersEmail author E.?Jacobsen R.?G.?F.?Visser 《In vitro cellular & developmental biology. Plant》2005,41(4):505-510
Summary An efficient system for the regeneration of plants from protoplasts was developed in Alstroemeria. Friable embryogenic callus (FEC) proved to be the best source for protoplast isolation and culture when compared with leaf
tissue and compact embryogenic callus. Protoplast isolation was most efficient when FEC was cultured under vacuum for 5 min
in an enzyme solution consisting of 4% cellulase, 0.5% Driselase and 0.2% Macerozyme, followed by culture for 12–16h in the
dark at 24°C. Cell wall formation and colony formation were better in a liquid medium than on a semi-solid agarose medium.
Micro-calluses were formed after 4 wk of culture. Ninety percent of the micro-calluses developed into FEC after 12wk of culture
on proliferation medium. FEC cultures produced somatic embryos on a regeneration medium and half of these somatic embryos
developed shoots. Protoplast-derived plants showed more somaclonal variation than vegetatively propagated control plants. 相似文献
46.
Deepak?PremEmail author Subhadra?Singh Padam?Prakash?Gupta Jaivir?Singh Sish?Pal?Singh?Kadyan 《Plant Cell, Tissue and Organ Culture》2005,80(2):209-214
Guar (Cyamopsis tetragonoloba L. Taub) is a drought tolerant and multipurpose grain legume cash crop grown primarily under rainfed conditions in several countries. The effect of various growth regulators and their combinations on a variety of explants, namely the embryo, cotyledons, cotyledonary nodes, shoot tip and hypocotyle, has been studied and an efficient system for callus induction and regeneration from callus has been developed. It was established that Murashige and Skoogs culture medium containing 2,4-dichlorophenoxyacetic acid (10.0M) in combination with 6-benzylaminopurine (5.0M) with embryo or cotyledon explants is most suitable for induction of green and friable morphogenic callus, with a range of 82.5–95% of cultured explants responding to callus induction. Efficient de novo shoot regeneration was achieved by culturing the callus obtained on this medium on Murashige and Skoogs medium containing 1-naphthlenacetic acid (13.0M) in combination with 6-benzylaminopurine (5.0M) with a range of 82.1–88.4% of callus clumps producing 20–25 shoots. In vitro rooting of cultured shoots was obtained on half-salt concentration of Murashige and Skoogs culture medium supplied with indole-3-butyric acid (5.0M) on which 82–90% of cultured shoots produced healthy roots. The in vitro regenerated plants were grown to pod setting and subsequent maturity under greenhouse conditions. 相似文献
47.
48.
以可在黑龙江地区露地越冬的5个现代月季(Rosa chinensis)品种为实验材料,分别以其无菌苗的叶片和茎段为外植体,研究了愈伤组织诱导及植株再生方法。实验结果表明:5个寒地月季品种的叶片和茎段均可诱导出愈伤组织,2,4-D诱导愈伤组织的效果较好,高浓度的细胞分裂素不适合用于月季叶片和茎段愈伤组织的诱导;TDZ在月季愈伤组织分化培养过程中具有重要作用,光照培养可促进月季愈伤组织的分化,愈伤组织的分化能力随着继代次数的增加呈下降趋势。该实验成功地从2004-8和2004-9(2个月季品种)愈伤组织中诱导出再生植株,其愈伤组织的分化率分别为45%和38%。 相似文献
49.
辣木富含多种营养成分,在食品和药物开发方面有巨大的潜在开发价值。本文提供了一种可行的辣木细胞悬浮培养技术。由辣木的根诱导形成愈伤组织和叶诱导形成愈伤组织的合适细胞悬浮培养条件分别为MS培养基(MS)+1.0mg/L 2,4-二氯苯氧乙酸(2,4-D)+1.0mg/L激动素(KT)和MS+0.5mg/L 2,4-D+0.5mg/L KT,摇床转速均为50~100r/min,将愈伤组织添加到液体悬浮培养基中20d左右可得到大量悬浮细胞。本研究为辣木细胞水平的培养和研究提供了一条途径,为辣木潜在价值的开发利用提供新的思路。 相似文献
50.
Many attempts on optimization of sorghum [Sorghum bicolor (L.)
Moench] tissue culture induction media have been made, but the culture system
remains with some bottlenecks compared to that of other crops. This study aimed
at assessing the suitability of various induction media to produce embryogenic
callus (yellow and friable) with high induction rates and reduced phenolic
exudation. The six culture medium modifications: 3 based on Murashige and
Skoog (MS) medium and one each based on Chu N6, Gamborg B5 and 190-2
media respectively were applied in the culture of mature embryos from 10
sorghum genotypes. Although there was a genotype influence on the attainment
of a yellow callus, friability of the callus was determined to be dependent on the
culture medium and not the genotype. Half strength MS medium with 0.2 mg/l
2,4-D with 2.8 g/l Gelrite® as the gelling agent modified with 1.0 g/l KH2PO4,
1.0 g/l L-proline, 1.0 g/l L-asparagine and 0.16 mg/l CuSO4·5H2O (type E) was
found to be the most effective resulting in about 60% yellow coloured callus
induction with 25% friability. Addition of CuSO4·5H2O, KH2PO4, L-proline and
L-asparagine significantly reduced the phenolic production. Half strength MS
medium was observed to contribute to quality callus production when compared
to full strength MS media modified with the compounds. The half strength MS
medium was also observed to suppress phenolic production. Medium 190-2
produced the highest regeneration frequency (40%) among the 3-regeneration
media tested. The results provide information on a suitable sorghum callus
induction medium necessary for embryogenesis. 相似文献