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131.
Increased lipid production of the marine oleaginous microalgae Isochrysis zhangjiangensis (Chrysophyta) by nitrogen supplement 总被引:1,自引:0,他引:1
Effects of nitrate feeding on the cell growth and lipid accumulation of marine microalgae Isochrysis zhangjiangensis were investigated. When nitrate was supplied at interval of 24 h, instead of 72 h, a high lipid content of 40.9% and a biomass density of 3.1 g L−1 were obtained. To confirm whether I. zhangjiangensis accumulates lipid during nitrogen-repletion, a two-stage cultivation method was applied. This algal strain had a high lipid content during sustained nitrate addition and showed a high carbohydrate content under nitrate-depletion conditions. These results revealed that this algal strain can accumulate lipids under nitrogen-repletion conditions and accumulate carbohydrate under nitrogen-depletion conditions. When cultured in an extremely high nitrate concentration, 9 g L−1 at 24 h intervals, the growth of algal cells was suppressed, but the highest lipid content of 53% was attained. This special characteristic of lipid accumulation makes I. zhangjiangensis an ideal candidate for producing biodiesel using N-rich wastewater. 相似文献
132.
Xu Y Huang J Warren A Al-Rasheid KA Al-Farraj SA Song W 《European journal of protistology》2011,47(3):172-185
The morphology and infraciliature of three karyorelictean ciliates, Geleia sinica spec. nov. and two poorly known Kentrophoros species, K. flavus and K. gracilis, isolated from the intertidal zone of a beach at Qingdao, China, were investigated. Geleia sinica spec. nov. is distinguished from its congeners by the following combination of characters: body medium-sized and slender-cylindrical; with a conspicuous prebuccal fossa; 28–34 somatic kineties; about 40 short adoral polykineties; intrabuccal kinety composed of 25–34 dikinetids; paroral kineties composed of closely spaced dikinetids. The comparison with similar congeners clearly supports the validity of this new species based on morphological and small subunit (SSU) rRNA gene sequence data. In light of these new data the “well-known” morphotype, Geleia simplex (Fauré-Fremiet, 1951), is redefined. Two Kentrophoros species are redescribed and improved diagnoses are supplied. Kentrophoros flavus Raikov and Kovaleva, 1968 is mainly characterized by having about 33 macronuclei and 12 micronuclei forming a row that extends along the cell meridian, and 12–19 ciliary rows on the right side of the cell. Kentrophoros gracilis Raikov, 1963 is characterized by having about 14 macronuclei, 13 micronuclei and 10–13 kineties on the right side of the cell. 相似文献
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135.
Two new sorbicillinoids, 1 and 2 , together with a novel benzofuranone derivative named phialofurone ( 3 ), were isolated from a deep‐sea sediment‐derived fungus, Phialocephala sp. Their structures were established on the basis of spectroscopic data. All compounds displayed cytotoxic effects against P388 (IC50 values of 11.5±1.4, 0.1±0.1, and 0.2±0.01 μM , resp.) and K562 (IC50 values of 22.9±0.8, 4.8±0.3 and 22.4±0.9 μM , resp.) cell lines. 相似文献
136.
Quenouille B Hubert N Bermingham E Planes S 《Molecular phylogenetics and evolution》2011,58(3):546-552
We investigated whether present distribution ranges in marine organisms are reliable indicators of the geographic pattern of past speciation events by assessing the level of geographic overlap (sympatric index) as a function of node age in four phylogenies of tropical marine species groups. The analyses led to remarkably similar results among the four groups examined with (1) most nodes associated with a sympatry index of either 0 (allopatry) or 1 (entire overlap) and (2) statistical support that sister species have an allopatric distribution significantly more frequently than sister clades (i.e. groups of species). Species divergences were expressed on a time scale and very similar times were needed for species range overlap to occur since sharp transitions from allopatry to sympatry occurred around 4 Ma in all groups. Present results supports that species range changes were not random as previous simulations results supports that species range probably evolve through occasional shifts of large amplitude. In front of the time needed for species range overlapping, our study suggests that species interaction and competitive exclusion can no longer be excluded as a driver of marine species distribution. 相似文献
137.
由于海洋环境和微藻本身的特殊性,从微藻中筛选抗菌物质已成为当今研究的热点之一,并且具有广阔的应用前景。对近年来从微藻中筛选抗菌物质的研究现状和进展进行了综述,重点总结了抗菌活性物质筛选过程中的模型选用,并介绍了新筛选模型的发展,指出了难点问题及可行的解决方法。 相似文献
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Michael Cunliffe 《The ISME journal》2011,5(4):685-691
The Marine Roseobacter Clade (MRC) is a numerically and biogeochemically significant component of the bacterioplankton. Annotation of multiple MRC genomes has revealed that an abundance of carbon monoxide dehydrogenase (CODH) cox genes are present, subsequently implying a role for the MRC in marine CO cycling. The cox genes fall into two distinct forms based on sequence analysis of the coxL gene; forms I and II. The two forms are unevenly distributed across the MRC genomes. Most (18/29) of the MRC genomes contain only the putative form II coxL gene. Only 10 of the 29 MRC genomes analysed have both the putative form II and the definitive form I coxL. None have only the form I coxL. Genes previously shown to be required for post-translational maturation of the form I CODH enzyme are absent from the MRC genomes containing only form II. Subsequent analyses of a subset of nine MRC strains revealed that only MRC strains with both coxL forms are able to oxidise CO. 相似文献
140.
The identification of inteins in viral genomes is becoming increasingly common. Inteins are selfish DNA elements found within coding regions of host proteins. Following translation, they catalyse their own excision and the formation of a peptide bond between the flanking protein regions. Many inteins also display homing endonuclease function. Here, the newly identified coccolithovirus intein is described and is predicted to have both self-splicing and homing endonuclease activity. The biochemical mechanism of its protein splicing activity is hypothesised, and the prevalence of the intein among natural coccolithovirus isolates is tested. 相似文献