全文获取类型
收费全文 | 372篇 |
免费 | 9篇 |
国内免费 | 6篇 |
出版年
2023年 | 4篇 |
2022年 | 4篇 |
2021年 | 8篇 |
2020年 | 7篇 |
2019年 | 17篇 |
2018年 | 23篇 |
2017年 | 5篇 |
2016年 | 1篇 |
2015年 | 5篇 |
2014年 | 23篇 |
2013年 | 36篇 |
2012年 | 23篇 |
2011年 | 37篇 |
2010年 | 28篇 |
2009年 | 35篇 |
2008年 | 25篇 |
2007年 | 24篇 |
2006年 | 19篇 |
2005年 | 11篇 |
2004年 | 9篇 |
2003年 | 7篇 |
2002年 | 5篇 |
2000年 | 4篇 |
1999年 | 5篇 |
1998年 | 2篇 |
1997年 | 3篇 |
1996年 | 2篇 |
1993年 | 1篇 |
1985年 | 2篇 |
1984年 | 2篇 |
1983年 | 2篇 |
1982年 | 1篇 |
1981年 | 1篇 |
1980年 | 2篇 |
1978年 | 2篇 |
1974年 | 1篇 |
1973年 | 1篇 |
排序方式: 共有387条查询结果,搜索用时 203 毫秒
381.
382.
383.
Vidhi Chaudhary Vishal Gupta Shashi B. Singh Chitra Natarajan Lata Nain 《Archives Of Phytopathology And Plant Protection》2013,46(14):1435-1444
An investigation was undertaken to optimise the microtitre plate-based assay for undertaking in-depth analyses of the potency and mode of action of cyanobacterial metabolites exhibiting fungicidal activity. The 96-well titre plate, using potato dextrose agar medium was standardised for evaluating minimum inhibitory concentration (MIC) of cyanobacterial metabolites against several phytopathogenic fungi, in terms of volume of media, concentration/volume of metabolite, inoculum and wavelength to be used for scanning. The optimised protocol was employed for recording growth inhibition in terms of MIC and facilitating microscopic analyses of morphological abnormalities induced by cyanobacterial metabolites in the fungal hyphae. This study not only illustrated the utility of the newly developed titre plate assay for analyses of large number of samples simultaneously and but also represented a first time report on microscopic observations related to various facets of fungicidal activity exhibited by cyanobacterial metabolites. Future research is directed towards scale up of this method for studies on tripartite interactions of cyanobacterial metabolites, target fungi with selected host plants, as a prelude to their use as biocontrol agents. 相似文献
384.
AGATA SIEDLECKA MIRELA J. WOLF-BACA KATARZYNA PIEKARSKA 《Polish journal of microbiology》2021,70(1):57
Although antibiotic-resistant bacteria (ARB) have been isolated from tap water worldwide, the knowledge of their resistance patterns is still scarce. Both horizontal and vertical gene transfer has been suggested to contribute to the resistance spread among tap water bacteria. In this study, ARB were isolated from finished water collected at two independent water treatment plants (WTPs) and tap water collected at several point-of-use taps during summer and winter sampling campaigns. A total of 24 strains were identified to genus or species level and subjected to antibiotic and disinfectant susceptibility testing. The investigated tap water ARB belonged to phyla Proteobacteria, Bacteroidetes, Actinobacteria, and Firmicutes. The majority of the isolates proved multidrug resistant and resistant to chemical disinfectant. Neither seasonal nor WTP-dependent variabilities in antibiotic or disinfectant resistance were found. Antibiotics most effective against the investigated isolates included imipenem, tetracyclines, erythromycin, and least effective – aztreonam, cefotaxime, amoxicillin, and ceftazidime. The most resistant strains originate from Afipia sp. and Methylobacterium sp. Comparing resistance patterns of isolated tap water ARB with literature reports concerning the same genera or species confirms intra-genus or even intra-specific variabilities of environmental bacteria. Neither species-specific nor acquired resistance can be excluded. 相似文献
385.
Pedro Latorre-Muro Katherine E. O’Malley Christopher F. Bennett Elizabeth A. Perry Eduardo Balsa Clint D.J. Tavares Mark Jedrychowski Steven P. Gygi Pere Puigserver 《Cell metabolism》2021,33(3):598-614.e7
- Download : Download high-res image (250KB)
- Download : Download full-size image
386.
Bacillus thuringiensis subsp israelensis (Bti) and subsp kurstaki (Btk); (2) vegetative cells derived from these BT products; and (3) Gram-positive and Gram-negative bacteria used as controls.
The XTT-kinetic assay improved sensitivity and early reading of MIC breakpoints. The conventional colony count method for
determining minimal bactericidal concentration (MBC) was used to validate a multi-sample dot-blot assay in which organisms
in individual MIC assays are trapped free of residual antibiotic and their viability is estimated by in situ conversion of MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] to insoluble formazan. Tolerance (MBC/MIC)
for most antibiotics was low (≤4). Resistance to β-lactams was attributed to β-lactamase activity in both BT products and
cultures derived from them. MIC and MBC breakpoints in spore-based assays were also approximated by changes in genome copy,
using δ-endotoxin and β-lactamase genes as probes. The DNA assays are effective for monitoring and authenticating organisms
in microbe-containing biotechnology products.
Received 29 September 1998/ Accepted in revised form 12 February 1999 相似文献
387.
Control of cell selectivity of antimicrobial peptides 总被引:1,自引:0,他引:1
Katsumi Matsuzaki 《生物化学与生物物理学报:生物膜》2009,1788(8):1687-1081
Antimicrobial peptides (AMPs) are promising novel antibiotics, because they exhibit broad antimicrobial spectra and do not easily induce resistance. For clinical applications, it is important to develop potent AMPs with less toxicity against host cells. This review article summarizes the molecular basis for the cell selectivity (bacteria versus host cells) of AMPs and various attempts to control it, including the optimization of physicochemical parameters of peptides, the introduction of d-, fluorinated, and unusual amino acids into peptides, the constraining of peptide conformations, and the modification of peptides by polymers. Pros and cons of these approaches are discussed. 相似文献