兰索拉唑与奥美拉唑治疗活动期胃溃疡的效果及对血清抗氧化因子的影响

目的:观察和比较兰索拉唑与奥美拉唑治疗活动期胃溃疡的临床效果及其对患者血清抗氧化因子水平的影响。方法:选取2016年1月至2017年1月我院收治的活动期胃溃疡患者88例,将其随机分成研究组和对照组,每组各44例。对照组给予奥美拉唑于睡前口服治疗,一次20mg,每日1次。研究组给予兰索拉唑于睡前口服治疗,一次30mg,每日1次。两组患者均以7d为一个疗程,连续治疗8个疗程。比较两组患者的临床总有效率和治疗前后血清超氧化物歧化酶(Superoxide dismutase,SOD)、丙二醛(Methylene dioxyamphetamine,MDA)、内皮素-1(Endothelin-1,ET-1)和一氧化氮(Nitric oxide,NO)水平的变化。结果:(1)研究组治疗总有效率为97.73%,对照组治疗总有效率为79.55%,研究组治疗总有效率显著高于对照组(P0.05);(2)治疗前,两组患者血清SOD、MDA、ET-1和NO水平比较差异无统计学意义(P0.05),研究组患者治疗后血清MDA、ET-1水平均明显低于对照组,血清SOD、NO水平均显著高于对照组(P0.05)。结论:兰索拉唑治疗活动期胃溃疡的临床效果明显优于奥美拉唑,可能与其显著提高患者血清SOD、NO水平及降低血清MDA、ET-1水平有关。     

Development of antler-type fruiting bodies of Ganoderma lucidum and determination of its biochemical properties

Following the importance of antler-type fruiting bodies of Ganoderma lucidum, in this study, the impact of main growth parameters such as ventilation and light on the development of antler-type fruiting bodies has been investigated together with the determination of physico-chemical properties of antler fruiting bodies. For this, the primordia bags of G. lucidum were kept under controlled ventilation to adjust the CO₂ produced by the mushrooms owing to its respiration under light and dark conditions. The bioactive compounds such as phenolics, flavonoids, water-soluble polysaccharides and ganoderic acid showed a two-fold increase in the antler-type fruiting bodies as compared to normal kidney-shaped fruiting bodies. It is assumed from this study that the antler type fruiting bodies are developed due to restricted ventilation which causes an increase in the level of CO₂ gas in the air as a result of respiration of mushroom. The shape and colour of antler fruiting bodies again dependent on the light provided in the growth chamber. This study also proves that with the manipulation of light and ventilation antler-type fruiting bodies of G. lucidum could be developed with higher quantity of bioactive compounds and with higher antioxidant potential.     

千里光等五种中草药的体外抑菌实验

本文对千里光、野菊花、地锦草、龙葵、马齿苋五种中草药分别进行体外抑菌实验。观察结果表明,千里光等五种中草药煎剂对金黄色葡萄球菌,白喉杆菌、伤寒杆菌、大肠杆菌、变形杆菌和痢疾杆菌显示程度不等的抑制作用,以千里光、马齿苋、地锦草三味药的抑菌作用更为明显。本研究为判断和解释千里光等药物的清热解毒药理作用提供了有益的资料。     

低强度激光血管照射治疗脑损伤的实验研究

本实验采用ＳＤ大鼠脑损伤模型,设对照组和照射组,采用低能量半导体激光器对大鼠股静脉进行血管外照射,水迷宫试验测定大鼠记忆功能,并测定脑组织和血ＳＯＤ、ＭＤＡ含量。结果发现低强度激光血管照射能促进照射组记忆功能的恢复,使ＳＯＤ活性提高,ＭＤＡ含量降低。这提示低强度激光照射改善了脑损伤后脑组织的缺血缺氧状态,从而减轻了氧自由基反应造成的继发性脑损害     

力竭性游泳对大鼠胃组织丙二醛,游离巯基和ATP含量的影响

目的：探讨力竭性游泳造成胃肠功能紊乱的机理。方法：３２只ＳＤ雄性大鼠随机分为４组,即对照组（Ｃ）;运动后即刻组（ＥＸ１）;运动后３０ｍｉｎ组（ＥＸ２）;运动后６０ｍｉｎ组（ＥＸ３）,测定力竭性游泳后不同时相胃组织中ＭＤＡ,游离巯基和ＡＴＰ含量。结果：运动后即刻三项指标没有发生显著变化,但在运动３０ｍｉｎ和６０ｍｉｎ组中ＭＤＡ含量显著增加,而游离巯基和ＡＴＰ含量在这两个时相中显著下降,同时,解剖学观察也证实,力竭性游泳后３０ｍｉｎ和６０ｍｉｎ胃组织损伤严重。结论：运动源性自由基对胃组织有严重损伤,是造成运动性胃肠功能紊乱的重要因素。     

The Growth,physiological and biochemical response of foxtail millet to atrazine herbicide

Foxtail millet (Pennisetum glaucum L.) is a vital crop that is planted as food and fodder crop around the globe. There is only limited information is present for abiotic stresses on the physiological responses to atrazine. A field experiment was conducted to investigate the effects of different atrazine dosages on the growth, fluorescence and physiological parameters i.e., malonaldehyde (MDA) and reactive oxygen species (ROS) (H₂O₂ and O₂) in the leaves to know the extent of atrazine on oxidative damage of foxtail millet. Our experiment consisted of 0, 2.5, 12.5, 22.5 and 32.5 (mg/kg) of labeled atrazine doses on 2 foxtaill millet varieties. High doses of atrazine significantly enhanced ROS and MDA synthesis in the plant leaves. Enzymes activities like ascorbate peroxidase (APX) and peroxidase (POD) activities enhanced, while catalase (CAD) and superoxide dismutase (SOD) activities reduced with increasing atrazine concentrations. Finally atrazine doses at 32.5 mg/kg reduced chlorophyll contents, while chlorophyll (a/b) ratio also enhanced. Biomass, plant height, chlorophyll fluorescence parameters, minimal and maximal fluorescence (Fo, Fm), maximum and actual quantum yield, photochemical quenching coefficient, and electron transport rate are decreased with increasing atrazine doses.     

甘肃鼢鼠哈氏腺组织结构及其抗氧化能力

为探讨甘肃鼢鼠(Myospalax cansus)哈氏腺的结构特征及其在低氧应激下的抗氧化能力,用组织解剖学方法观察甘肃鼢鼠哈氏腺整体及其显微结构,分光光度计测定哈氏腺低氧应激前、后超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽还原酶(GR)活性及丙二醛(MDA)含量。结果显示,甘肃鼢鼠哈氏腺肥大,包围在眼周,位于颧骨下的颞窝,为管泡状腺体,由柱状细胞构成,依胞质中分泌物含量分为厚细胞和薄细胞。常氧下,甘肃鼢鼠超氧化物歧化酶和过氧化氢酶活性显著高于SD大鼠(Rattus norvegicus),但谷胱甘肽还原酶活性显著低于SD大鼠;在低氧应激4 h后,甘肃鼢鼠超氧化物歧化酶和过氧化氢酶活性迅速升高,显著高于SD大鼠,谷胱甘肽还原酶的活性在低氧2、4和6 h无显著性变化,但均显著低于SD大鼠;在低氧8 h后,甘肃鼢鼠谷胱甘肽还原酶的活性较低氧2~6 h显著升高。甘肃鼢鼠丙二醛含量在常氧和低氧应激中均显著低于SD大鼠。结果说明,甘肃鼢鼠在低氧应激后,哈氏腺通过提高抗氧化酶超氧化物歧化酶和过氧化氢酶活性,清除低氧诱导产生的多余自由基,谷胱甘肽还原酶在抗氧化中不起主要作用。地下鼠甘肃鼢鼠抗氧化模式与地面鼠明显不同。     

杂交稻及其三系叶片衰老过程中SOD、CAT活性和MDA含量的变化

对杂交水稻及其三系主茎第11叶叶片自然衰老过程中超氧物歧化酶(SOD)、过氧化氢酶(CAT)和丙二醛(MDA)含量的变化进行了研究,结果表明:叶片衰老过程中,SOD和CAT活性下降,MDA的含量增加,可作为衰老特征的叶绿素和可溶性蛋白质含量明显下降;SOD的活性和MDA的含量变化相对应;CAT活性大幅度下降与SOD之间的不平衡,致使O_2~-代谢中间产物累积而引起膜的损伤。不育系的衰老进程比杂交水稻、恢复系和保持系慢,其SOD和CAT活性明显高于其它三者,可能是不育系不易早衰的原因之一。