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71.
Summary Effects of increasing rates of lime (0, 900, 1725, and 3000 kg Ca(OH)2/ha producing soil pH of 4.0, 4.7, 5.1 and 5.6) and P (50, 150, 250 and 350 kg P/ha) on top and root yield, root morphology
and chemical composition of lotus (Lotus pedunculatus Cav.) and white clover (Trifolium repens L.), were studied, using an acid soil in a greenhouse experiment.
Increasing rates of applied lime and phosphate resulted in substantial increases in top yields of both species but concomitant
increases in root yield were small. In the unlimed soil, lotus out-yielded (tops and roots) white clover at all P levels.
However, in the three limed treatments, white clover clearly out-yielded lotus. Yield response curves to applied P levelled
off at the two highest lime rates for lotus but not for white clover.
Nodulation and N content of white clover increased significantly with increasing lime applications, but for lotus there was
a significant decrease in nodulation at the highest lime rate. Increased P rates had a small stimulatory effect on nodulation
in both species.
Of the total root weight, the percentage contribution of the tap and primary lateral root fractions was smaller and that of
the secondary plus tertiary lateral roots was greater for lotus than for white clover although root length per unit weight
tended to be larger for white clover at the two highest lime rates. Furthermore, lotus possessed longer and more numerous
root hairs than white clover. Lime applications significantly decreased the percentage contribution of the tap and primary
lateral roots to the total root weight and increased the percentage contribution of the secondary plus tertiary lateral roots.
Al and Mn contents of tops and roots of both species decreased with increasing lime rates. There was a highly significant
negative correlation between relative yield and Al content of lotus and white clover tops. In comparison with the limed treatments,
in the unlimed treatments a greater percentage of total P, Al, Mn and N content accumulated in the roots of both species.
In addition, lotus accumulated a much greater percentage Al in its roots than white clover. 相似文献
72.
73.
Fukai E Soyano T Umehara Y Nakayama S Hirakawa H Tabata S Sato S Hayashi M 《The Plant journal : for cell and molecular biology》2012,69(4):720-730
We established a gene tagging population of the model legume Lotus japonicus using an endogenous long terminal repeat (LTR) retrotransposon Lotus Retrotransposon 1 (LORE1). The population was composed of 2450 plant lines, from which a total of 4532 flanking sequence tags of LORE1 were recovered by pyrosequencing. The two-dimensional arrangement of the plant population, together with the use of multiple identifier sequences in the primers used to amplify the flanking regions, made it possible to trace insertions back to the original plant lines. The large-scale detection of new LORE1 insertion sites revealed a preference for genic regions, especially in exons of protein-coding genes, which is an interesting feature to consider in the interaction between host genomes and chromoviruses, to which LORE1 belongs, a class of retrotransposon widely distributed among plants. Forward screening of the symbiotic mutants from the population succeeded to identify five symbiotic mutants of known genes. These data suggest that LORE1 is robust as a genetic tool. 相似文献
74.
Jinqiu Liao Sylvia Singh Stig U. Andersen Loretta Ross Dario Bonetta Yonghong Zhou Shusei Sato Satoshi Tabata Jens Stougaard Krzysztof Szczyglowski Martin Parniske 《The Plant journal : for cell and molecular biology》2012,72(4):572-584
One of the earliest responses of legumes to symbiotic signalling is oscillation of the calcium concentration in the nucleoplasm of root epidermal cells. Integration and decoding of the calcium‐spiking signal involve a calcium‐ and calmodulin‐dependent protein kinase (CCaMK) and its phosphorylation substrates, such as CYCLOPS. Here we describe the Lotus japonicus ccamk‐14 mutant that originated from a har1‐1 suppressor screen. The ccamk‐14 mutation causes a serine to asparagine substitution at position 337 located within the calmodulin binding site, which we determined to be an in vitro phosphorylation site in CCaMK. We show that ccamk‐14 exerts cell‐specific effects on symbiosis. The mutant is characterized by an increased frequency of epidermal infections and significantly compromised cortical infections by Mesorhizobium loti and also the arbuscular mycorrhiza fungus Rhizophagus irregularis. The S337 residue is conserved across angiosperm CCaMKs, and testing discrete substitutions at this site showed that it participates in a negative regulation of CCaMK activity, which is required for the cell‐type‐specific integration of symbiotic signalling. 相似文献
75.
Strigolactones: chemical signals for fungal symbionts and parasitic weeds in plant roots 总被引:12,自引:0,他引:12
• Aims Arbuscular mycorrhizae are formed between >80 % of land plants and arbuscular mycorrhizal (AM) fungi. This Botanical Briefing highlights the chemical identification of strigolactones as a host-recognition signal for AM fungi, and their role in the establishment of arbuscular mycorrhizae as well as in the seed germination of parasitic weeds.• Scope Hyphal branching has long been described as the first morphological event in host recognition by AM fungi during the pre-infection stages. Host roots release signalling molecules called ‘branching factors’ that induce extensive hyphal branching in AM fungi. Strigolactones exuded from host roots have recently been identified as an inducer of hyphal branching in AM fungi. Strigolactones are a group of sesquiterpenes, previously isolated as seed germination stimulants for the parasitic weeds Striga and Orobanche. Parasitic weeds might find their potential hosts by detecting strigolactones, which are released from plant roots upon phosphate deficiency in communication with AM fungi. In addition to acting as a signalling molecule, strigolactones might stimulate the production of fungal symbiotic signals called ‘Myc factors’ in AM fungi.• Conclusions Isolation and identification of plant symbiotic signals open up new ways for studying the molecular basis of plant–AM-fungus interactions. This discovery provides a clear answer to a long-standing question in parasitic plant biology: what is the natural role for germination stimulants? It could also provide a new strategy for the management and control of beneficial fungal symbionts and of devastating parasitic weeds in agriculture and natural ecosystems. 相似文献
76.
Ten rhizobial strains isolated from Lotus sp. have been characterized by their ability to denitrify. Out of the 10 strains, the five slow-growing isolates grew well under oxygen-limiting conditions with nitrate as a sole nitrogen source, and accumulated nitrous oxide in the growth medium when acetylene was used to inhibit nitrous oxide reductase activity. All five strains contained DNA homologous to the Bradyrhizobium japonicum nirK, norBDQ and nosZ genes. In contrast, fast-growing lotus rhizobia were incapable of growing under nitrate-respiring conditions, and did not accumulate nitrous oxide in the growth medium. DNA from each of the five fast-growing strains showed a hybridization band with the B. japonicum nirK gene but not with norBDQ and nosZ genes. Partial 16S rDNA gene sequencing revealed that fast-growing strains could be identified as Mesorhizobium loti species and the slow-growers as Bradyrhizobium sp. 相似文献
77.
78.
Background and Aims
Two main strategies that allow plants to cope with soil waterlogging or deeper submergence are: (1) escaping by means of upward shoot elongation or (2) remaining quiescent underwater. This study investigates these strategies in Lotus tenuis, a forage legume of increasing importance in areas prone to soil waterlogging, shallow submergence or complete submergence.Methods
Plants of L. tenuis were subjected for 30 d to well-drained (control), waterlogged (water-saturated soil), partially submerged (6 cm water depth) and completely submerged conditions. Plant responses assessed were tissue porosity, shoot number and length, biomass and utilization of water-soluble carbohydrates (WSCs) and starch in the crown.Key Results
Lotus tenuis adjusted its strategy depending on the depth of submergence. Root growth of partially submerged plants ceased and carbon allocation prioritized shoot lengthening (32 cm vs. 24·5 cm under other treatments), without depleting carbohydrate reserves to sustain the faster growth. These plants also developed more shoot and root porosity. In contrast, completely submerged plants became quiescent, with no associated biomass accumulation, new shoot production or shoot elongation. In addition, tissue porosity was not enhanced. The survival of completely submerged plants is attributed to consumption of WSCs and starch reserves from crowns (concentrations 50–75 % less than in other treatments).Conclusions
The forage legume L. tenuis has the flexibility either to escape from partial submergence by elongating its shoot more vigorously to avoid becoming totally submerged or to adopt a non-elongating quiescent strategy when completely immersed that is based on utilizing stored reserves. The possession of these alternative survival strategies helps to explain the success of L. tenuis in environments subjected to unpredictable flooding depths. 相似文献79.
Woranuch La-ongsri Chusie Trisonthi Henrik Balslev 《Wetlands Ecology and Management》2009,17(4):279-289
Management and use of Nelumbo nucifera Gaertn.—the lotus plant—was studied in 58 wetlands distributed throughout Thailand. Although traditionally harvested in extractive
systems depending on natural wetlands, N. nucifera is now increasingly being managed. Two hundred eighty informants mentioned 20 different uses, mainly for food, medicine,
and religious rites, in both subsistence and cash economies. The uses of N. nucifera appeared to be rather generalized throughout the country even if informants in the northern and central regions knew of more
uses and ways of commercializing its products, possibly related to the abundance of wetlands and natural stand in those regions,
and maybe also cultural differences. 相似文献
80.
超表达AVP1基因提高转基因百脉根的耐盐性和抗旱性 总被引:1,自引:0,他引:1
本研究以超表达拟南芥液泡膜H+-焦磷酸酶编码基因AVPI的转基因百脉根为材料,对其耐盐性和抗旱性进行了检测。结果显示:在200mmol·L^-1 NaCl下处理或自然干旱7d后,转基因植株的生长虽然受到抑制,但受抑程度明显低于野生型植株,前者叶片相对含水量比后者分别高18%和14%,净光合速率分别高20%和21%,而MDA含量则分别低35%和27%,相对质膜透性分别低28%和27%。此外,随着盐和干旱胁迫的加剧,与野生型植株相比,转基因植株体内积累了更多Na+、K+和Ca2+。以上结果表明,AVPI基因的超表达可能提高了百脉根细胞Na+区域化能力,既减轻了过量Na+对细胞质的毒害作用,也提高了植株的渗透调节能力,从而增强了百脉根的耐盐性和抗旱性。 相似文献