全文获取类型
收费全文 | 284篇 |
免费 | 11篇 |
国内免费 | 2篇 |
出版年
2023年 | 1篇 |
2022年 | 2篇 |
2021年 | 3篇 |
2019年 | 4篇 |
2018年 | 4篇 |
2017年 | 2篇 |
2016年 | 6篇 |
2015年 | 6篇 |
2014年 | 11篇 |
2013年 | 22篇 |
2012年 | 8篇 |
2011年 | 13篇 |
2010年 | 6篇 |
2009年 | 11篇 |
2008年 | 12篇 |
2007年 | 14篇 |
2006年 | 14篇 |
2005年 | 17篇 |
2004年 | 13篇 |
2003年 | 12篇 |
2002年 | 6篇 |
2001年 | 6篇 |
2000年 | 14篇 |
1999年 | 8篇 |
1998年 | 6篇 |
1997年 | 15篇 |
1996年 | 6篇 |
1995年 | 2篇 |
1994年 | 8篇 |
1993年 | 3篇 |
1992年 | 3篇 |
1991年 | 3篇 |
1990年 | 13篇 |
1989年 | 4篇 |
1988年 | 2篇 |
1987年 | 2篇 |
1985年 | 5篇 |
1984年 | 2篇 |
1983年 | 2篇 |
1981年 | 4篇 |
1980年 | 1篇 |
1976年 | 1篇 |
排序方式: 共有297条查询结果,搜索用时 31 毫秒
61.
莲种子萌发和幼苗生长时期营养物质的代谢变化 总被引:1,自引:1,他引:1
莲子叶细胞中储存了丰富的营养物质,主要为蛋白质、淀粉和淀粉质体DNA.这些贮藏物质为种子萌发和幼苗的生长提供必需的能量和养料.通过组织化学和显微镜观察,研究莲从种子萌发到植株生长至具有4个节时,子叶中贮藏物质消耗的全过程.在此过程中,子叶中的贮藏物质不断降解,营养物质发生转运.蛋白体首先发生降解,其大量降解主要发生在幼苗三叶期.淀粉质体降解时会聚集成团,之后体积逐渐减小,最后完全降解.种子萌发后65天是子叶贮藏物质消耗末期,淀粉质体DNA的含量比萌发后20天的三叶期明显减少.细胞壁的形态结构发生多种形式的变化,细胞壁发生的这些变化与子叶细胞间物质的运输有关.含多糖的球形颗粒通过维管束在子叶中运输. 相似文献
62.
63.
Losses of soil N through leaching and N2 fixation by legumes often are related to soil nitrate concentration. The seasonal distribution of soil ammonium and nitrate
concentrations under ungrazed legume-grass and grass swards were evaluated on two experiments that were established in 1983
(Exp. 1) and in 1984 (Exp. 2). Treatments were white clover (Trifolium repens L.) (WC), red clover (Trifolium pratense L.) (RC), and birdsfoot trefoil (Lotus corniculatus L.) (BT), each grown with tall fescue (Festuca arundicacea Schreb.) (TF) at two legume proportions, and a pure stand of TF. The concentrations of both forms of N were measured in the
top 20-cm layer during 2 years in Exp. 1 and for 1 year in Exp. 2. The concentrations of nitrate and ammonium were least in
winter and spring, and greatest in summer. The concentration of nitrate for the mixtures decreased in the order WC-TF, RC-TF,
and BT-TF in both summers of Exp. 1 but there were no mixture differences in Exp. 2. The concentration of soil ammonium was
not affected by the treatments applied. We conclude that the concentration of soil nitrate usually was small for these swards
but became greater and often dependent on species and legume proportion during summer. The concentration of soil ammonium
also was greater in summer but was not affected by species or legume proportion.
Journal of Paper no. J.-13359 of the Iowa Agric. and Home Econ. Exp Stn., Ames. Project 2281. Supported in part by the Facultad
de Agronomía, Montevideo, Uruguay.
Journal of Paper no. J.-13359 of the Iowa Agric. and Home Econ. Exp Stn., Ames. Project 2281. Supported in part by the Facultad
de Agronomía, Montevideo, Uruguay. 相似文献
64.
Fulvio Pupilli Francesco Damiani Elena Nenz Sergio Arcioni 《In vitro cellular & developmental biology. Plant》1992,28(4):167-171
Summary Nodes ofMedicago sativa, Lotus corniculatus, Lotus tenuis, andLotus pedunculatus were cultured on MS basal media with different growth regulators. InM. sativa each node produced one shoot and the apical dominance was unaffected by high levels of cytokinins, and subsequent cycles
of culture. Shoot development was stimulated by the presence ofN
6-isopentenyl-adenine in the culture medium and was dependent on the genotype of the explant. Shoot development was not affected
by the original position of the node on the plant nor by the plant age. Shoots rooted in MS medium gelled with starch and
containing 2 mg·liter−1 indol-3-acetic acid. In the threeLotus species, node culture was a more effective technique than inM. sativa. The number of shoots per node increased with the time of culture and with the presence of 0.05 mg·liter−1 of 6-benzylaminopurine. The highest number of shoots derived from one node was achieved inL. pedunculatus and inL. tenuis by culturing single nodes, whereas inL. corniculatus stem segments had to be totally covered by the medium for success. Rooting was easily achieved in MS medium with or without
auxins. 相似文献
65.
Birgitte Skadhauge Margaret Y. Gruber Karl Kristian Thomsen Diter von Wettstein 《American journal of botany》1997,84(4):494-503
Proanthocyanidin (PA) and anthocyanin accumulation and location in developing leaves, flowers, and seeds of the legumes Medicago sativa, Lotus japonicus, Lotus uliginosus, Hedysarum sulfurescens, and Robinia pseudacacia were investigated by quantitative measurements and by histological analysis after staining with 1% vanillin/HCl, butanol/HCl, or 50% HCl. M. sativa leaves and flowers, L. japonicus leaves, and R. pseudacacia flowers do not contain PAs, but seeds of all investigated species contain PAs. Anthocyanins are absent in the seed coats of all five species and in leaves of L. japonicus. PA content generally increases as a function of development in leaves, but declines in flowers. With the exception of H. sulfurescens, flower PAs are synthesized in the parenchyma cells of the standard petal, while anthocyanins are located in the neighboring epidermal cells. Leucocyanidin reductase (LCR) catalyzes the conversion of 2,3-trans-3,4-cis-leucocyanidin to (+)-catechin and is the first enzyme in the PA-specific pathway. LCR activity was only detected in PA-containing tissues and generally declined during tissue development. 相似文献
66.
Bavage Adrian D. Davies Ian G. Robbins Mark P. Morris Phillip 《Plant molecular biology》1997,35(4):443-458
Condensed tannins (proanthocyanidins) are an important factor in the nutritive and dietary quality of many forage crops. We report here experiments aimed at altering the levels and monomer composition of condensed tannins (CTs) in hairy root cultures of Lotus corniculatus (bird's foot trefoil) using genetic manipulation. An Antirrhinum majus dihydroflavonol reductase (DFR) cDNA was expressed in sense in L. corniculatus and CT levels in transgenic root cultures were analysed. Two co-transformed lines were noted with decreased CT content relative to controls and these levels were comparable with antisense-DFR phenotypes. In ADFR10, a co-transformed line with the highest CT levels, CT structure was altered in a manner consistent with the substrate specificity of the introduced gene; that is an increase in pro-pelargonidin monomers noted after hydrolysis of CTs. RT-PCR confirmed the expression of endogenous DFR gene(s) in both putatively co-suppressed lines and also in ADFR10. Analysis of selected root culture lines indicated that the monomer composition of CTs did not change during growth and development but that levels of CTs varied in a regulated manner. 相似文献
67.
Lotus tenuis is a perennial legume with a good adaptation to infertile, heavy and waterlogging soils. It can replace alfalfa in these sites with a similar feeding value. An important constraint is its weak competitive capacity with other graminae and weed species in permanent pastures, having consequently a poor forage yield. The objective of the present research is to overcome this disadvantage, enhancing its competitive ability with foliar applications of GA3 (GA) and phosphorus (P), increasing L. tenuis forage yield. Field experiments were conducted during 1994 with foliar application of GA (50 mg.l-1) and during 1995 with foliar application of GA (25 and 50 mg.l-1), phosphorus (8 kg.ha-1, as P2O5) and their combinations, in permanent pastures with L. tenuis and other companion grasses. In 1994 GA 50 increased significantly L. tenuis dry matter (DML) in 64.6% but not the dry matter of graminae fraction (DMG) and in consequence the total dry matter of the pasture (TDMP) was increased. In 1995 all GA treatments and their combinations with phosphorus enhanced DML but not DMG. In this sense GA 25 + P was the most effective treatment with a 151% increment of DML. Consequently TDMP was significantly increased due to a larger participation of L. tenuis in the forage yield. This increase was achieved due to a greater length and diameter of L. tenuis branches, with a logical modification in leaf:stem ratio. Moreover GA treatments reduced L. tenuis flower number. Phosphorus treatment, applied alone, showed an increase in the DML. GA treatments did not modify the feeding value of the forage in L. tenuis and graminae fractions, except GA 50 and GA 50 + P in acid detergent fiber (ADF), neutral detergent fiber (NDF) and crude protein (CP), respect to the control. The total crude protein (CP.m-2) was enhanced in all GA and GA + P treatments. Foliar GA3 and phosphorus spray applications increased the competitiveness of trefoil for light, on account of morphological changes in the spatial disposition of L. tenuis stems reaching faster the top of the pasture canopy. This practice can be an adequate alternative to increase the forage yield and total crude protein in permanent and cultivated pastures with a low cost-benefit ratio. 相似文献
68.
百脉根愈伤组织原生质体再生植株 总被引:1,自引:0,他引:1
百脉根无菌苗幼茎在含2.0mg/L-,2,4-D,0.1mg/L2-ip的MS培养基上诱导和继代培养愈伤组织。选取绿色松散颗粒愈伤组织分离原生质体。原生质体培养在调整珠KM8P,V-KM,MS和SH培养基上「含300mg/L,CH,2%CW,2%蔗糖,6%葡萄糖,2.0mg/L,2,4-D,0.5mgg/L,BA,5mmol/L MES」,原生质体再生细胞均能分裂,并形成小愈伤组织,但以KM80为 相似文献
69.
Effects of extreme weather events and legume presence on mycorrhization of Plantago lanceolata and Holcus lanatus in the field
下载免费PDF全文
![点击此处可从《Plant biology (Stuttgart, Germany)》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Little is known about direct and indirect effects of extreme weather events on arbuscular mycorrhizal fungi (AMF) under field conditions. In a field experiment, we investigated the response of mycorrhization to drought and heavy rain in grassland communities. We quantified AMF biomass in soil, mycorrhization of roots of the grass Holcus lanatus and the forb Plantago lanceolata, as well as plant performance. Plants were grown in four‐species communities with or without a legume. We hypothesised that drought increases and heavy rain decreases mycorrhization, and that higher mycorrhization will be linked to improved stress resistance and higher biomass production. Soil AMF biomass increased under both weather extremes. Heavy rain generally benefitted plants and increased arbuscules in P. lanceolata. Drought neither reduced plant performance nor root mycorrhization. Arbuscules increased in H. lanatus several weeks after drought, and in P. lanceolata several weeks after heavy rain spells. These long‐lasting effects of weather events on mycorrhization highlight the indirect influence of climate on AMF via their host plant. Legume presence increased plant community biomass, but had only minor effects on mycorrhization. Arbuscule colonisation was negatively correlated with senescence during the dry summer. Mycorrhization and biomass production in P. lanceolata were positively related. However, increased mycorrhization was related to less biomass in the grass. AMF mycelium in soil might generally increase under extreme events, root colonisation, however, is host species specific. This might amplify community shifts in grassland under climate change by further increasing stress resistance of species that already benefit from changed precipitation. 相似文献
70.
Anna Małolepszy Terry Mun Niels Sandal Vikas Gupta Manu Dubin Dorian Urbański Niraj Shah Asger Bachmann Eigo Fukai Hideki Hirakawa Satoshi Tabata Marcin Nadzieja Katharina Markmann Junyi Su Yosuke Umehara Takashi Soyano Akira Miyahara Shusei Sato Makoto Hayashi Jens Stougaard Stig U. Andersen 《The Plant journal : for cell and molecular biology》2016,88(2):306-317
Long terminal repeat (LTR) retrotransposons are closely related to retroviruses, and their activities shape eukaryotic genomes. Here, we present a complete Lotus japonicus insertion mutant collection generated by identification of 640 653 new insertion events following de novo activation of the LTR element Lotus retrotransposon 1 (LORE1) ( http://lotus.au.dk ). Insertion preferences are critical for effective gene targeting, and we exploit our large dataset to analyse LTR element characteristics in this context. We infer the mechanism that generates the consensus palindromes typical of retroviral and LTR retrotransposon insertion sites, identify a short relaxed insertion site motif, and demonstrate selective integration into CHG‐hypomethylated genes. These characteristics result in a steep increase in deleterious mutation rate following activation, and allow LORE1 active gene targeting to approach saturation within a population of 134 682 L. japonicus lines. We suggest that saturation mutagenesis using endogenous LTR retrotransposons with germinal activity can be used as a general and cost‐efficient strategy for generation of non‐transgenic mutant collections for unrestricted use in plant research. 相似文献