小叶栎一新变种

本文发表了小叶栎一新变种, 即临安栎Quercus chenii Nakaivar.linanensis M.C.Liu et X.L.Shen, var.nov.。     

昆明地区早寒武世三叶形虫新属——Panlongia Liu et Luo gen. nov.

简述早寒武世关山动物群的研究现状及重要意义。重点描述该动物群中一三叶形虫新属PanlongiaLiuetLuogen.nov.及其两个新种:即个体小、拥有等大的头甲和尾甲、边缘没有明显突出刺状物的PanlongiatetranodusaLiuetLuogen.etsp.nov.及头甲后侧和尾缘具刺的PanlongiaspinosaLiuetLuogen.etsp.nov.。三叶形虫在关山动物群中的首次发现为寒武纪生命大爆发以及古地理、古板块恢复的研究提供了有益的证据。     

中国荞麦属（蓼科）一新种——密毛野荞麦

描述了蓼科一新种——密毛野荞麦（Fagopyrum densovillosum J. L. Liu）,本种与细柄野荞麦（F. gracilipes(Hemsl.) Damm. et Diels）相近似,不同在于植株全体密被白色直立长毛,茎枝较粗壮,节较密集,节间较短, 叶较大,长（0.9～）1.7～6 cm,宽（0.7～）1.2～5.1 cm,阔卵形,心形,阔心形,阔卵状心形,卵形,长卵形,三角状卵形或卵状三角形,在上面具细皱纹,明显小泡状突起,叶柄较长,长 (0.5～) 2.6～7.5 cm,果实较小,长1.8～2.5 mm,直径1.5～2 mm,易于区别。     

枣镰翅小卷蛾性信息素通讯系统

采用单个雌蛾性信息素腺体分析技术对枣镰翅小卷蛾Ancylis sativa Liu雌蛾性信息素的组分和组分间的精确比例进行了测定,结果表明,枣镰翅小卷蛾雌蛾的性信息素系统由二个顺反异构体组分组成,即反-9-十二碳烯醋酸酯(E9-12∶Ac)和顺-9-十二碳烯醋酸酯(Z9-12∶Ac)组成,E9-12∶Ac与Z9-12∶Ac的比例为6.5∶3.5;雌蛾产生和释放性信息素具有时辰节律性,在光周期14L∶10D、温度为21℃时,性信息素产生的高峰期为进入黑暗期6.5 h;不同日龄雌蛾产生的性信息素有差异,1日龄最低,2日龄最高,3～5日龄居中,不同日龄雌蛾产生的性信息素组分间的比例无显著差异;对3个世代雌蛾产生的性信息素的量及组分间的比例的研究表明,越冬代含量最高,为(10.1±7.0)ng/头,第2代为(9.5±4.6)ng/头,第1代仅为(1.4±1.0)ng/头,3个世代性信息素组分间的比例无显著差异,在63.1%～64.3%(E)范围内。     

大凉疣螈碱性磷酸酶的分离纯化及部分性质

碱性磷酸酶 (alkaline phosphatase,AKP)在生物界的分布很广 ,动物、植物、微生物中均广泛存在 .提纯的 AKP常被应用于对核酸等的研究 ,是基因工程常用的工具酶 ,也是酶标免疫测定技术的常用工具酶之一 .人类血清中的 AKP在不同疾病状态下有显著变化 ,临床上将血清 AKP变化指标作为诊断某些疾病的依据 .对于细菌和高等动物的 AKP已有广泛的研究 ,但国内外对两栖爬行类动物 AKP的研究报导却很少 ,仅有蛇毒中 AKP的研究报导 [1,2 ] .本文对大凉疣螈皮肤的 AKP进行了分离纯化 ,并对其部分性质进行了初步研究 .1　材料和方法1 .1　材…     

危害黄杉球果的实小卷蛾属一新种(鳞翅目:卷蛾科)(英文)

记述了实小卷蛾属 (小卷蛾亚科 ,花小卷蛾族 )的一个新种 ,黄杉实小卷蛾RetiniapseudotsugaicolaLiuetWu ,sp .nov .。其幼虫危害黄杉PseudotsugasinensisDode的球果 ,在云南省禄劝县云龙乡球果受害率达 80 %～ 90 %。该新种雄性外生殖器的抱器瓣在腹面中央凹陷很深 ,抱器腹拐角几乎呈直角 ,抱器端的腹角尖 ,可与该属其它种相区别。该新种的雌性外生殖器与松实小卷蛾R .cristata很相似 ,但成虫的花纹完全不同 ,该新种前翅的中横带很宽 ,且布满了灰绿色和灰黄色的云状纹 ,而松实小卷蛾前翅的中横带则较窄 ,翅端有明显的肛上纹。在昆明禄劝县一年发生 1代 ,以蛹在球果中越冬。越冬蛹于翌年 3月下旬开始羽化。 4月上旬至 7月下旬是幼虫危害期。老熟幼虫 7月下旬开始陆续化蛹越冬。提供了成虫外形、翅脉、雌雄外生殖器解剖图及幼虫的毛序图。模式标本保存在中国科学院动物研究所     

Use of Pulsed Low–Dose Rate Radiotherapy in Refractory Malignancies

BACKGROUND: Most tumor cell lines exhibited low-dose hyperradiosensitivity (LDHRS) to radiation doses lower than 0.3 Gy. Pulsed low–dose rate radiotherapy (PLDR) took advantage of LDHRS and maximized the tumor control process. In this study, we retrospectively analyzed patients receiving PLDR for refractory malignancies. PATIENTS AND METHODS: In total, 22 patients were included in our study: 9 females and 13 males. The median age was 61 years old. All the patients previously received multiline treatments and failed with an estimated survival less than 6 months. Thus, palliative PLDR was given. The PLDR was delivered using 10 fractions of 2 Gy/day, with an interval of 3 minutes, for 5 days per week. The dose rate was 6.67 cGy/min. The median follow-up was 1 year (range 8-30 months). Nine patients underwent PLDR for reirradiation due to locally recurrent diseases. The time interval from last irradiation was 11 to 168 months. Ten patients received PLDR due to poor performance status. Three patients were given PLDR for bulky tumor. The irradiated sites included primary disease (seven patients), locally recurrent disease (nine patients), and retroperitoneal adenopathy (six patients). RESULTS: Five patients developed grade 3 or 4 toxicities. No grade 5 toxicities occurred. All the toxicities recovered after treatments. In general, the 1-year local-regional control rate was approximately 40%, and almost all the patients developed progression at the second year after PLDR. The 6-month survival rate was 76%, and the 1-year survival rate was 69%. For the three patients given PLDR for bulky tumor, all of them achieved partial remission 1 month after the PLDR, and one patient achieved complete response at the fourth month. CONCLUSION: PLDR is an effective and safe option not only for reirradiation but also for patients with poor performance status or bulky tumors. A prospective clinical trial (NCT03061162) is ongoing to validate our results.     

The Chemokine Receptor CXCR4 and c-MET Cooperatively Promote Epithelial-Mesenchymal Transition in Gastric Cancer Cells

The C-X-C motif chemokine receptor 4 (CXCR4) pathway can promote tumor metastasis but is dependent on cross talk with other signaling pathways. The MET proto-oncogene (c-MET) participates in metastasis and is highly expressed in gastric cancer. However, the relationship between CXCR4 and c-MET signaling and their mechanisms of action in gastric cancer metastasis remain unclear. In this study, in vitro experiments demonstrated that C-X-C motif chemokine ligand 12 (CXCL12)/CXCR4 induces epithelial-mesenchymal transition (EMT) and promotes migration in gastric cancer cells, which is accompanied by c-MET activation. These phenomena were reversed by c-MET inhibition. Further investigation revealed that c-MET activation correlated with its interaction with caveolin 1 in lipid rafts, induced by CXCL12. In clinical samples, we observed a significant positive association between CXCR4 expression and c-MET phosphorylation (r = 0.259, P = .005). Moreover, samples expressing both receptors were found to indicate significantly poorer patient prognosis (P < .001). These results suggest that CXCL12 induces EMT at least partially through cross talk between CXCR4 and c-MET signaling. In addition, changes in these pathways could have clinical importance for the treatment of gastric cancer.     

推拿联合简易六字诀功法治疗抽动障碍患者的疗效及安全性分析

摘要 目的：探究推拿联合简易六字诀功法治疗抽动障碍患者的疗效及安全性分析。方法：选择2021年3月至2022年1月于上海中医药大学附属岳阳中西医结合医院就诊于推拿科,并通过病史询问、量表评估被确诊为抽动障碍的患者。本研究随机分为对照组（n=27）及联合组（n=31）2组,对照组进行单纯推拿治疗,联合组进行推拿联合六字诀功法治疗。根据耶鲁综合抽动严重程度量表对患儿治疗前后的运动性抽动、发声性抽动以及社会行为影响作出评估,根据尼莫地平法计算患儿治疗前后疗效指数,统计患儿治疗的不良事件。结果：（1）两组患儿一般临床资料对比无差异（P>0.05）。（2）联合组总有效率高于对照组（P<0.05）。（3）治疗前联合组与对照组发声性抽动评分、缺损评分无差异,治疗后联合组发声性抽动评分、缺损评分明显高于对照组,两组治疗后均明显低于治疗前（P<0.05）。（4）治疗前后联合组与对照组YGTSS总分、运动性抽动评分无差异,两组治疗后YGTSS总分与运动性抽动评分均明显低于治疗前,且联合组低于对照组（P<0.05）。结论：推拿和简易六字诀功法对抽动障碍患儿都有显著的治疗效果,且效果无差异,与单纯推拿相比,推拿联合简易六字诀功法更能降低患儿的缺损评分,减少抽动对患儿自尊、家庭生活、社交和学习的负面影响,值得临床借鉴。