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271.
D. Koedam H. H. W. Velthuis† M. R. Dohmen‡ V. L. Imperatriz-Fonseca 《Physiological Entomology》2001,26(3):254-259
Abstract. As in many other stingless bees, Melipona bicolor bicolor Lepeletier (Apidae: Meliponinae) workers lay two morphologically distinct types of eggs: slender ones that have a typical patterned chorion, and larger ones that lack this pattern. In this paper we report on the relation between egg morphology and the behaviour of the workers that lay such eggs. In most cases, the laying of each of these egg types is accompanied by a unique sequence of behaviours. After a worker has laid the unpatterned type of egg, she generally leaves the cell, giving the queen the possibility of eating this egg. In the case of the patterned egg type, the worker usually closes the cell immediately after her egg laying. When worker egg laying occurs right after a series of regurgitations, it stops the queen from ovipositing. When, instead, a worker lays an egg after queen oviposition, the cell contains two eggs. This study also revealed cases in which workers laid slender, patterned eggs without closing the cell, and other cases where workers laid large, spherical, unpatterned eggs and instantly closed the cell. Experiments in which worker eggs, destined to be eaten by the queen, were protected by covering the cell artificially with a piece of wax showed that some of these eggs developed into larvae, although they were occasionally relatively small. The occurrence of a range of combinations of egg-laying behaviours and egg morphologies in M. b. bicolor workers is discussed from the perspective of worker egg laying in other stingless bees. 相似文献
272.
Betaine accumulation in salt-stressed sorghum 总被引:5,自引:0,他引:5
Two analytical methods for measuring betaine were compared in a study of betaine accumulation in salt-stressed sorghum. Spectrophotometric determination of betaine as the p -bromophenacyl ester is highly sensitive and specific. However, a periodide assay was found to be more convenient for screening numerous plant samples without undue sacrifice in accuracy.
The accumulation of betaine in grain sorghum [ Sorghum bicolor (L.) Moench] cvs NK 265 and Double TX was measured in salt-stressed plants grown hydroponically and in the field and in drought-stressed potted plants. Neither drought nor mild salinity (-0.2 MPa) stress was effective in stimulating betaine accumulation. However, when the osmotic potential of the culture solution was lowered to -0.8 MPa, betaine levels in the shoots rose rapidly for 12 days after initiation of salination, and then declined, apparently because of dilution by plant growth. In young leaf blades, betaine was strongly accumulated up to 70–75 μmol (g dry weight)−1 ; the concentration in leaf sheaths was less than 6 μmol (g dry weight)−1 .
In the field, betaine levels in salt-stressed sorghum increased 6- to 7-fold over the basal level of the control plants. In a comparable study of two wheat species ( Triticum aestivum L. cv. Probred and T. durum Desf. cv. 1000-D), betaine increased only 3- to 4-fold over unstressed plants. 相似文献
The accumulation of betaine in grain sorghum [ Sorghum bicolor (L.) Moench] cvs NK 265 and Double TX was measured in salt-stressed plants grown hydroponically and in the field and in drought-stressed potted plants. Neither drought nor mild salinity (-0.2 MPa) stress was effective in stimulating betaine accumulation. However, when the osmotic potential of the culture solution was lowered to -0.8 MPa, betaine levels in the shoots rose rapidly for 12 days after initiation of salination, and then declined, apparently because of dilution by plant growth. In young leaf blades, betaine was strongly accumulated up to 70–75 μmol (g dry weight)
In the field, betaine levels in salt-stressed sorghum increased 6- to 7-fold over the basal level of the control plants. In a comparable study of two wheat species ( Triticum aestivum L. cv. Probred and T. durum Desf. cv. 1000-D), betaine increased only 3- to 4-fold over unstressed plants. 相似文献
273.
The influence of vegetation on erosion and accretion in salt marshes of the Oosterschelde,The Netherlands 总被引:1,自引:0,他引:1
Martha M. van Eerdt 《Plant Ecology》1985,62(1-3):367-373
The total root strength of two plant species (Spartina anglica and Limonium vulgare) is related to salt marsh cliff erosion in the Krabbenkreek (Oosterschelde). A ranking order in cliff stability is predicted on the basis of these root strength calculations. It turns out that the S. anglica root system is more effective in reducing lateral cliff erosion than the root system of L. vulgare. Also the establishment of S. anglica by germination of seeds is studied in relation to the erosion/deposition rates at an accretion site in the Krabbenkreek. The percentage of seeds washed away depends on the mobility of the superficial sediment which increases with decreasing height above N.A.P. (Dutch Ordnance Level). Above 0.90 m + N.A.P. a germination of 20% is measured, but seedlings survive the winter period only in the zone where a patchy vegetation already exists. It is concluded that generative spread of S. anglica in the Krabbenkreek is not very likely under the present hydrodynamic conditions.Nomenclature follows Heukels & van Ooststroom (1977).Acknowledgement: The research presented herein was carried out at the Delta Department, Environmental Division of the Ministry of Transport and Public Works, Middelburg. This work forms part of the research on salt-marsh ecosystems of the Oosterschelde. Assistance with sowing and sampling was given by the Field Survey section of the Environmental Division. Analyses of root weight and root diameter were carried out by the Institute of Soil Fertility in Haren by order of the Environmental Division. I gratefully acknowledge Ir A. de Jager and J. Floris of this institute for putting a special sampling auger at my disposal. G. den Hartog, P. van Vessem and Drs P. M. Schoot of the State University of Utrecht are thanked for their assistance during the study. Special thanks are due to Drs J. M. Roels for this critical editorial review of this paper. 相似文献
274.
Abstract The germination of Sorghum bicolor seeds of 9 genotypes was tested at temperatures between 8°C and 48°C on a thermal gradient plate. Samples were tested from three regions of the panicle expected to differ in temperature during grain filling. Seeds of a tenth genotype, SPV 354, produced in controlled-environment glasshouses at different panicle temperatures, were tested similarly. In addition, the emergence of SPV 354 was measured from planting depths of 2 and 5 cm at mean soil temperatures of 15, 20 and 25°C. Four methods of calculating mean germination rate for the nine genotypes were compared. Germination characters like base, optimum and maximum temperature (Tb, To, Tm), thermal time (θ)and the germination rate at To(Rmax showed only small differences between methods. There was a range of genotypic variation in all characters: Tb 8.5–11.9°C; To, 33.2–37.5°C; Tm, 46.8–49.2°C; θ, 23.4–38.0°Cd; Rmax, 0.69–1.14-d-1. In contrast, mean germinability (G) was between 90% and 100% over the temperature range 13–40°C. Panicle temperature had no effect on any germination character in SPV 354. However, deeper burial increased θ for emergence and decreased G, irrespective of soil temperature except at 5 cm. Increasing panicle temperature, by reducing seed size, reduced G and increased θ by about 10% only at 15°C and 5 cm depth. 相似文献
275.
Water deficit, leaf rolling and susceptibility to photoinhibition in field grown sorghum 总被引:2,自引:0,他引:2
Chlorophyll fluorescence and gas-exchange techniques were used to investigate changes in photosynthelic performance in response to high light and mild water deficit, in two cultivars of the C., plant sorghum ( Sorghurn bicofor [L.] Moench). grown under field conditions. For all leaves fully exposed to the sun, the efficiency of phcttosystem 11 (PSII) showed a mid-day decline, hut with substantial over-night recovery: the magnitude of the mid-day decline was enhanced by water deficit. There was no corresponding decline in leaves not exposed to full sunlight, either because they were shaded by other leaves or else because of leaf-roiling. Net assimilation rates appeared more sensitive to water-deficit than was PSI1 efficiency. Shade-adapted leaves had lower rates of photosynthesis in full sun (and lower stomatal conductances) than well-exposed leaves. When these shade-adapted leaves were suddenly exposed to full sunlight, fluorescence quenching was slow. especially when plants were well-watered. For the latter, photochemical quenching (qp )was small even after several minutes. indicating a continuing imbalance between energy funnelled to PSI1 and subsequent electron transport. Shade-adapted leaves that were water stressed were better able to withstand a sudden increase in irradiance than those that were well watered. It is suggested that the shade-adapted eaves from unirrigated plants. having a lower s'tomatal conductance than the irrigated leaves, had been acclimated by receiving energy in excess of that required to fix CO2 , thus leading to the operation of dissipative mechanisms. A shortened protocol for quenching analysis is proposed that enables non-photochemical quenching to be partitioned into rapidly and slowly relaxing components (the latter including photoinhibition) by relating results to a theoretical maximum yield of variable fluorescence. This is particularly suitable for screening field material. 相似文献
276.
Michael P. Schwarz 《Evolution; international journal of organic evolution》1994,48(5):1684-1697
Montane populations of the Australian allodapine bee, Exoneura bicolor, are characterized by high levels of cooperative nesting and strongly female-biased sex ratios. A conspecific population from heathland shows much lower levels of cooperative nesting and lower levels of female bias. In both habitats, sex-ratio bias is greatest in the smallest brood sizes and becomes successively less biased in larger broods. Parity is approached in the largest heathland colonies, but not for any brood-size category in montane areas. Adult intracolony relatedness is moderately high for colonies in both reused and newly founded nests in the montane habitat, but probably low or zero for newly founded nests in heathland. Colony efficiency, measured as the number of brood per adult, increases with colony size in both habitats, suggesting that cooperation between females increases mean female fitness. It is argued that patterns of sex allocation are consistent with nonlinear fitness-return models, in which the mean reproductive value of daughters increases with the number of daughters produced in a brood. Such increases probably arise from a number of social interactions, including cooperative brood defense, increased task efficiency, and lower per capita costs in nest construction. The term “local fitness enhancement” is introduced here to describe these effects collectively. The female-biased ratios should lower selective thresholds for sib-directed altruism, at least in the earlier stages of colony development. It is argued that local fitness enhancement facilitates eusociality in allodapine bees and could also play a role in other haplodiploid taxa, provided cooperative nesting largely involves sisters, colony efficiency increases with colony size, and optimal colony sizes are only achieved after two or more generations after founding. 相似文献
277.
Cobaltous ions (Co2+ ) inhibited light-mediated anthocyanin synthesis and phenylalanine ammonia-lyase (PAL; EC 4. 3. 1. 5) activity in excised first internodes of Sorghum bicolor (L.) Moench. Ethephon (an exogenous source of ethylene) restored anthocyanin synthesis and PAL activity. Sulfhydryl-containing compounds like cysteine and glutathione completely restored anthocyanin synthesis and PAL activity. N-ethylmaleimide (NEM), a sulfhydryl reagent, inhibited anthocyanin synthesis and PAL activity; cysteine or glutathione reversed the effect of NEM. EDTA and other organic acids like citric, malic, oxalic and tartaric acid were also effective in bringing about recovery. It is suggested that the site of action of Co2+ might be at sulfhydryl groups or two adjacent carboxylic groups of a macromolecule that is involved either in anthocyanin or ethylene biosynthesis 相似文献
278.
279.
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