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Parthenogenesis, the development of female gametes without fertilization, is relatively common in brown algae, although limited quantitative information on the phenomenon is available. Its occurrence is reported for the first time in Lessonia nigrescens Bory, a member of the Laminariales and a key ecological component of the benthic algal communities along the Chilean coast. Isolated female gametophytes developed into parthenosporophytes throughout the year, with a maximum in spring to early summer. Isolated male gametophytes, on the other hand, never developed fronds. Parthenosporophytes obtained in the laboratory developed normally when cultivated under greenhouse conditions, and the resulting individuals were indistinguishable in size, shape, texture, and color from heterozygous sporophytes. Quantification of DNA of various tissues demonstrated that early during their development, parthenosporophytes duplicated their DNA content, displaying levels similar to heterozygous sporophytes and almost twice the level found in gametophytes. One out of 45 individuals from a field population yielded only female gametophytes, strongly suggesting that parthenogenesis does occur in wild stands of L. nigrescens. 相似文献
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Omondi AB Orantes LC VAN DEN Berg J Masiga D Schulthess F 《Molecular ecology resources》2009,9(4):1236-1239
Teretrius nigrescens is a predator of the larger grain borer (LGB) Prostephanus truncatus, an invasive post-harvest pest in Africa. We describe the isolation and characterization of 24 novel polymorphic microsatellite markers and their testing on a population from Honduras. Alleles per locus ranged between 2 and 12, and observed heterozygosity between 0.037 and 0.646. Six loci deviated significantly from Hardy-Weinberg equilibrium and showed evidence of null alleles. These markers will be useful for studies of the predator's population structure and characterizing populations for control of LGB. 相似文献
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The tropical African tree species Acacia nigrescens Oliv. was grown in environmentally controlled growth chambers at three CO2 concentrations representative of the Last Glacial Maximum (~180 ppmv), the present day (~380 ppmv), and likely mid‐21st century (~600 ppmv) CO2 concentrations. Isoprene (C5H8) emissions, per unit leaf area, were greater at lower‐than‐current CO2 levels and lower at higher‐than‐current CO2 levels relative to controls grown at 380 ppmv CO2. Changes in substrate availability and isoprene synthase (IspS) activity were identified as the mechanisms behind the observed leaf‐level emission response. In contrast, canopy‐scale emissions remained unaltered between the treatments as changes in leaf‐level emissions were offset by changes in biomass and leaf area. Substrate concentration and IspS activity‐CO2 responses were used in a biochemical model, coupled to existing isoprene emission algorithms, to model isoprene emissions from A. nigrescens grown for over 2 years at three different CO2 concentrations. The addition of the biochemical model allowed for the use of emission factors measured under present day CO2 concentrations across all three CO2 treatments. When isoprene emissions were measured from A. nigrescens in response to instantaneous changes in CO2 concentration, the biochemical model satisfactorily represented the observed response. Therefore, the effect of changes in atmospheric CO2 concentration on isoprene emission at any timescale can be modelled and predicted. 相似文献
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Amy B. Baird Timothy J. McCarthy Robert G. Trujillo Yuan Yuan Kang Mehdi Esmaeiliyan Joselyn Valdez 《分类学与生物多样性》2018,16(2):108-117
Small-eared shrews of the genus Cryptotis (Mammalia: Eulipotyphla: Soricidae) are widespread in the northern Neotropics. Systematic studies of these shrews over the past two decades have revealed previously undocumented morphological and species diversity, resulting in a quadrupling of the number of recognized species. Unfortunately, a small proportion of the species in the genus have been included in molecular phylogenetic studies, and evolutionary relationships within the genus are incompletely known. Traditionally, species have been assigned to four or five morphologically defined ‘species groups’, but tests of the monophyly of some of these groups show weak support and relationships amongst species groups remain somewhat speculative. The largest species group is the C. mexicanus group inhabiting Mexico and northern Central America. We studied sequences from mitochondrial cytochrome-b and 16S genes, as well as nuclear ApoB and BRCA1 genes from 22 species of Cryptotis, including 15 species in the C. mexicanus group. Our combined analysis shows that the C. goldmani subgroup is very weakly supported as monophyletic; however, the C. mexicanus group as a whole is not monophyletic. Our molecular phylogenetic analyses confirm the distinctiveness of two newly described species (C. celaque and C. mccarthyi) from isolated highlands of western Honduras and illustrate their relationship with other species formerly considered part of a widespread C. goodwini. 相似文献
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Question: Our study aimed at testing to what extent water and/or nutrients affect community composition in sub‐alpine heath. We hypothesized that nutrient addition will have an overall positive effect on heath vegetation but water addition will have greater effects and will probably reinforce the effects of fertilization in drier habitat conditions. Location: Monte Rondinaio, northern Apennines, Italy (44°08’ N; 10°35’ E, ca. 1850‐1930 m a.s.l.). Methods: Nutrients and water were added during five growing seasons in two communities (HV community, moister; EV community, drier) and the biomass of all species was estimated non‐destructively by the point‐quadrat method. Results: Total above‐ground biomass increased in both communities with fertilization due to increased graminoid biomass and decreased moss biomass, but was unaffected by irrigation. In the HV community species richness decreased as an effect of fertilization while in the EV community species richness was raised by irrigation. Conclusions: Our study partly supported our hypothesis since water addition had a stronger effect in the drier community. However, this effect was not revealed by changes in above‐ground biomass but rather by differing responses of individual species in the two communities. 相似文献
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A 40-kDa lactoferrin-binding protein was identified in a strain of Prevotella nigrescens isolated from a patient with periodontitis. The protein was purified by affinity column chromatography using a Sepharose–lactoferrin column and detergent-solubilized membranes. The N-terminal sequence revealed no apparent similarities with any other sequenced bacterial protein. The native conformation of the 40-kDa protein was a condition to bind either iron-free or iron-saturated lactoferrin. A possible function of this Lf-binding protein could be related with an iron acquisition mechanism in P. nigrescens. 相似文献
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Purification and biochemical characterization of gentisate 1,2-dioxygenase from Klebsiella pneumoniae M5a1 总被引:3,自引:0,他引:3
Abstract Type strains and 62 clinical isolates of Prevotella intermedia and Prevotella nigrescens were typed with the use of genomic DNA fingerprints and rRNA gene probes. The strains were further serotyped with monoclonal antibodies and characterized with SDS-PAGE, enzymatic activities, hemolysis and hemagglutination and coaggregation with Streptococcus and Actinomyces spp. P. intermedia and P. nigrescens were found to have distinct ribotype patterns which correspond to previously defined serotypes I and II/III, respectively. No clear phenotypic difference related to hemolysis, hemagglutination and coaggregation with Streptococcus and Actinomyces species, or expression of aminopeptides and lipase was found between P. intermedia and P. nigrescens . 相似文献
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Hemolytic and hemagglutinating activities of Prevotella intermedia and Prevotella nigrescens 总被引:1,自引:0,他引:1
Masaaki Okamoto Nobuko Maeda Kenichiro Kondo Kai-P. Leung 《FEMS microbiology letters》1999,178(2):299-304
A total of 91 isolates of Prevotella intermedia or Prevotella nigrescens from subgingival sites were identified by PCR using primers specific for sequences of 16S rRNA. The hemolytic and hemagglutinating activities of the P. intermedia isolates exhibited significantly higher levels compared to those of the P. nigrescens isolates by quantitative analysis. The hemagglutinin gene (phg) was found in 23 of 26 P. intermedia isolates (88.5%), whereas it was found in only two of 44 isolates (4.5%) of P. nigrescens. The high hemolytic and hemagglutinating activities of P. intermedia may be involved in the pathogenicity of P. intermedia in the progression of periodontal disease. 相似文献