全文获取类型
收费全文 | 32458篇 |
免费 | 1965篇 |
国内免费 | 4807篇 |
专业分类
39230篇 |
出版年
2024年 | 73篇 |
2023年 | 520篇 |
2022年 | 756篇 |
2021年 | 990篇 |
2020年 | 883篇 |
2019年 | 1139篇 |
2018年 | 910篇 |
2017年 | 866篇 |
2016年 | 911篇 |
2015年 | 1133篇 |
2014年 | 1532篇 |
2013年 | 2086篇 |
2012年 | 1500篇 |
2011年 | 1527篇 |
2010年 | 1330篇 |
2009年 | 1631篇 |
2008年 | 1787篇 |
2007年 | 1936篇 |
2006年 | 1975篇 |
2005年 | 1829篇 |
2004年 | 1694篇 |
2003年 | 1588篇 |
2002年 | 1461篇 |
2001年 | 1195篇 |
2000年 | 994篇 |
1999年 | 924篇 |
1998年 | 811篇 |
1997年 | 701篇 |
1996年 | 682篇 |
1995年 | 654篇 |
1994年 | 616篇 |
1993年 | 435篇 |
1992年 | 382篇 |
1991年 | 319篇 |
1990年 | 268篇 |
1989年 | 187篇 |
1988年 | 207篇 |
1987年 | 183篇 |
1986年 | 134篇 |
1985年 | 119篇 |
1984年 | 104篇 |
1983年 | 49篇 |
1982年 | 64篇 |
1981年 | 32篇 |
1980年 | 36篇 |
1979年 | 23篇 |
1978年 | 17篇 |
1977年 | 9篇 |
1976年 | 16篇 |
1950年 | 6篇 |
排序方式: 共有10000条查询结果,搜索用时 8 毫秒
101.
102.
SecA protein: Autoregulated initiator of secretory precursor protein translocation across theE. coli plasma membrane 总被引:10,自引:0,他引:10
Donald B. Oliver Robert J. Cabelli Gregory P. Jarosik 《Journal of bioenergetics and biomembranes》1990,22(3):311-336
Several classes ofsecA mutants have been isolated which reveal the essential role of this gene product forE. coli cell envelope protein secretion. SecA-dependent,in vitro protein translocation systems have been utilized to show that SecA is an essential, plasma membrane-associated, protein translocation factor, and that SecA's ATPase activity appears to play an essential but as yet undefined role in this process. Cell fractionation studies suggested that SecA protein is in a dynamic state within the cell, occurring in soluble, peripheral, and integral membraneous states. These data have been used to argue that SecA is likely to promote the initial insertion of secretory precursor proteins into the plasma membrane in a manner dependent on ATP hydrolysis. The protein secretion capability of the cell has been shown to translationally regulatesecA expression with SecA protein serving as an autogenous repressor, although the exact mechanism and purpose of this regulation need to be defined further. 相似文献
103.
借助于5'和3'末端删切后重建的IL-2R a链基因调控区次级克隆,在体外合成有放射性同位素参入的反意义RNA探针与总RNA进行液相杂交,结果表明TPA或PHA分别活化的T细胞在IL-2R a链表达过程中都在不同程度上有选择地利用了调控区内分别为-58(5')和+1(3')位两个转录起始点中3'转录起始点。热休克使PHA活化细胞更明显地利用+1位点。PHA诱导Jurkat细胞表达IL-2RamRNA斑点杂交证实,Jurkat细胞在活化16小时表达IL-2Ra基本达到高峰,至24小时已明显下降。根据这一规律提取PHA诱导活化15小时的Jurkat细胞S100和NE,进行有关结合蛋白的研究,初步结果显示磷酸纤维素柱的KCI洗脱组分中存在着DNA结合蛋白,有关结合蛋白性质的研究正在进行中。 相似文献
104.
韩玉珉 《中国生物化学与分子生物学报》1990,6(1):66-70
用寡聚核苷酸诱导的定位突变法,将人U_1和U_2snRNA基因的5'-端调控区域的一段能与SV_(40)T抗原相结合的DNA删去,造成缺失突变,改变这段DNA核苷酸的排列顺序,造成取代突变。突变株用原位杂交法筛选,由限制性内切酶电泳图谱分析和DNA顺序测定得到证实。突变率约为5%。 相似文献
105.
106.
107.
108.
Isozyme analysis of Galaxias species (Teleostei: Galaxiidae) from the Taieri River, South Island, New Zealand: a species complex revealed 总被引:2,自引:0,他引:2
Richard M. Allibone Todd A. Crowl Jean M. Holmes Tania M. King Robert M. McDowall Colin R. Townsend Graham P. Wallis 《Biological journal of the Linnean Society. Linnean Society of London》1996,57(2):107-127
We examined genetic differentiation among 23 samples of non-migratory river galaxias from 17 streams in the Taieri River system, South Island, New Zealand. Four major genetic types were found, two of which occur in narrow sympatry in one location. These were compared with topotypical material representing Galaxias anomalus from the Clutha system (Otago) and G. vulgaris from the Waimakariri system (Canterbury) in order to establish identity. Morphological examination of these four major genetic types revealed consistent concomitant differences. The results suggest that there are at least three species of river galaxias in the Taieri system: G. anomalus, G. vulgaris and at least one previously undescribed species. We propose that the genetic structuring and subsequent speciation of this group has been promoted by the absence of the marine juvenile phase that is found in five other members of the genus native to New Zealand. This structuring may be exacerbated by population fragmentation over the last century owing to the negative influence of introduced trout. The phylogenetic diversity within the river system mirrors the diverse flora and invertebrate fauna of the region, and has conservation implications that parallel those resulting from our improved knowledge of the New Zealand herpetofauna through the application of genetic analysis. 相似文献
109.
TheGNOM gene is required for pattern formation along the main body axis of the embryo in the flowering plantArabidopsis thaliana. Mutations in theGNOM gene alter the asymmetric division of the zygote and interfere with the formation of distinct apical-basal regions in the developing embryo. We have isolated theGNOM gene by positional cloning, characterised its structure and determined the molecular lesions in mutant alleles. Although the predicted 163 kDa GNOM protein has a conserved domain in common with the yeast secretory protein Sec7p, it is most closely related in size and overall similarity to the product of the yeastYEC2 gene, which is not essential for cell viability. Four fully complementinggnom alleles carry missense mutations in conserved regions, seven partially complementing alleles have premature stop codon mutations and two non-complementing alleles have splice-site lesions. Our results suggest that the GNOM protein acts as a complex of identical subunits and that partial complementation may involve low levels of full-length protein generated by inefficient translational read-through.Communicated by H. Saedler 相似文献
110.