首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   930篇
  免费   115篇
  国内免费   45篇
  1090篇
  2023年   15篇
  2022年   19篇
  2021年   19篇
  2020年   61篇
  2019年   46篇
  2018年   45篇
  2017年   44篇
  2016年   44篇
  2015年   39篇
  2014年   51篇
  2013年   69篇
  2012年   38篇
  2011年   43篇
  2010年   28篇
  2009年   55篇
  2008年   43篇
  2007年   47篇
  2006年   51篇
  2005年   35篇
  2004年   35篇
  2003年   41篇
  2002年   49篇
  2001年   35篇
  2000年   38篇
  1999年   25篇
  1998年   29篇
  1997年   18篇
  1996年   7篇
  1995年   4篇
  1994年   6篇
  1993年   1篇
  1992年   1篇
  1991年   2篇
  1990年   1篇
  1989年   1篇
  1988年   1篇
  1987年   1篇
  1984年   1篇
  1980年   1篇
  1978年   1篇
排序方式: 共有1090条查询结果,搜索用时 15 毫秒
101.
102.
103.
This study aims to assess the environmental impacts of canned sardines in olive oil, by considering fishing, processing, and packaging, using life cycle assessment (LCA) methodology. The case study concerns a product of a canning factory based in Portugal and packed in aluminum cans. It is the first LCA of a processed seafood product made with the traditional canning method. The production of both cans and olive oil are the most important process in the considered impact categories. The production of olives contributes to the high environmental load of olive oil, related to cultivation and harvesting phases. The production of aluminum cans is the most significant process for all impact categories, except ozone depletion potential and eutrophication potential, resulting from the high energy demand and the extraction of raw materials. To compare to other sardine products consumed in Portugal, such as frozen and fresh sardines, transport to the wholesaler and store was added. The environmental cost of canned sardines is almost seven times higher per kilogram of edible product. The main action to optimize the environmental performance of canned sardines is therefore to replace the packaging and diminish the olive oil losses as much as possible. Greenhouse gas emissions are reduced by half when plastic packaging is considered rather than aluminum. Frozen and fresh sardines represent much lower environmental impacts than canned sardines. Nevertheless, when other sardine products are not possible, it becomes feasible to use sardines for human consumption, preventing them from being wasted or used suboptimally as feed.  相似文献   
104.
SNUFER is a software for the automatic localization and generation of tables used for the presentation of single nucleotide polymorphisms (SNPs). After input of a fasta file containing the sequences to be analyzed, a multiple sequence alignment is generated using ClustalW ran inside SNUFER. The ClustalW output file is then used to generate a table which displays the SNPs detected in the aligned sequences and their degree of similarity. This table can be exported to Microsoft Word, Microsoft Excel or as a single text file, permitting further editing for publication. The software was written using Delphi 7 for programming and FireBird 2.0 for sequence database management. It is freely available for noncommercial use and can be downloaded from http://www.heranza.com.br/bioinformatica2.htm.  相似文献   
105.
Goal, scope and background  The industrial packages sector has great importance for the transport sector in Europe. These containers, mainly wooden pallets and spools, are subject to European legislation, which promotes their reuse and recycling. This study uses life cycle assessment (LCA) to assess the environmental impact of the current management system in this sector and the benefits and drawbacks of different reuse intensities as a waste prevention strategy as opposed to the recycling option. Materials and methods  In this paper, four case studies located in Spain and representative of the wooden package sector in Europe are analysed: high reuse pallet, low reuse pallet, low reuse spool and null reuse spool. For the LCA study cases, the functional unit is that required to satisfy the transport necessity of 1,000 t by road. The impact and energy consumption assessment methods used are CML 2 Baseline 2000 and Cumulative Energy Demand. Data are mostly provided by the leading enterprises and organisations in this sector. Results  The paper provides, as a first result, a comprehensive inventory of the systems under study. Secondly, our assessment shows that the systems with higher reuse intensity show a reduction in energy and wood consumption and all the environmental impact categories except for the global warming potential from 34.0% to 81.0% in the pallet study cases and from 50.4% to 72.8% in the spool ones. This reduction is at the expense of the maintenance stage, which on the contrary increases its impact, although it is still relatively small—less than 7% in all the impact categories and flow indicators of the study cases. The highest impact stages are transport, raw material extraction and the process chain. The final disposal and maintenance stages are the lowest impact, contributing at most to less than 30% of the impact in the pallet study cases and 10% in the spool cases. Discussion  Wood consumption (WC), directly related to the number of containers needed to satisfy the functional unit, is the main factor in determining the impact of the stages, especially in the raw materials extraction and process chain stages, assuming that these are undertaken with the same technologies in all the case studies. Other variables, such as the management system, the maintenance index and the final disposal scenario, affect the impact of the remaining stages: transport, maintenance and final disposal. The global warming potential results obtained demonstrate the environmental benefits of using containers made of a renewable resource such as wood instead of using other materials, but these results are not expected to prioritise the lower reuse systems because of their better performance in this category. Conclusions  Reuse, a strategy capable of reducing the environmental impacts of the wooden container systems, is preferable to recycling, while the package maintenance tasks are still feasible. Therefore, reuse, combined with recycling as final disposal, should be encouraged to reduce the demand for natural resources and the waste generated. Recommendations  Based on these results, attention should be paid to the maintenance stage, which, being the lowest-impact one, could substantially reduce the impact of the remaining stages.  相似文献   
106.
The carbon footprint (CF) of biofuels and biomaterials is a barrier to their acceptance, yet the greenhouse gas emissions associated with disposing of biomaterials are frequently omitted from analyses. This article investigates whether harmonization is appropriate for calculating the importance of biomaterials’ disposal. This research shows that disposal stages could double a biomaterial's CF, or reduce it to the point that it could claim to be zero carbon. Incineration with combined heat and power coupled with on‐site energy production in the biorefinery are identified as prerequisites to being zero carbon. The article assesses the current UK waste infrastructure's ability to support a low‐carbon bio‐based future economy, and finds that presently it only achieves marginal net reductions when compared to landfill and so cannot be said to support low‐carbon biomaterials, though the article challenges the polluter pays principle where low‐carbon disposal infrastructure are not available. Reuse and recycling are shown to have the potential to offset all the emissions caused by landfill of biomaterials. However, the savings are not so great as to offset the biomaterial's upstream emissions. The study explores the ability to overcome the barriers to incorporating disposal into life cycle assessment while identifying limitations of using harmonization as an assessment method. Specifically, data availability and industry consensus are flagged as major barriers. The study also uses sensitivity analysis to investigate the influence of methodological choices, such as allowing additional reuse and recycling stages, classifying biomaterials into different types, and choosing between opposing allocation methods.  相似文献   
107.
A wide variety of software tools are available to analyze microarray data. To identify the optimum software for any project, it is essential to define specific and essential criteria on which to evaluate the advantages of the key features. In this review we describe the results of our comparison of several software tools. We then conclude with a discussion of the subset of tools that are most commonly used and describe the features that would constitute the “ideal microarray analysis software suite.”  相似文献   
108.
MULDER (Mostly UniversaL Dihedral angle ExtractoR) is a program for extraction of torsion angle information from NMR data. Currently, it can analyze two types of input data: The torsion angle data, where several 3J-coupling constants and/or interatomic distances are combined in order to reduce the torsion angle ambiguity arising from solving the isolated Karplus (or distance) equation, and the sugar pucker data, where the dynamics of five-membered sugar rings is evaluated by postprocessing the results calculated from 3J(HH) coupling constants by program PSEUROT. Program MULDER can be used either as an alternative to r-MD programs in situations where only specific structural features are studied, or as a preparatory tool in connection with full r-MD structure calculation for extraction of unambiguous torsion angle restraints.  相似文献   
109.
For bioscientists studying protein structure and function, the Protein Family Alignment Annotation Tool (Pfaat) is a useful and simple program for annotating collections of proteins. This open-source software includes methods for viewing and aligning protein families, and for annotating sequence structure and residues with known functions. It offers new options to aid the study of proteins, and an extensible annotation tool for bioinformatics developers.  相似文献   
110.
Summary A new computer-based approach is described for efficient sequence-specific assignment of uniformly 15N-labeled proteins. For this purpose three-dimensional 15N-correlated [1H, 1H]-NOESY spectra are divided up into two-dimensional 1H-1H strips which extend over the entire spectral width along one dimension and have a width of ca. 100 Hz, centered about the amide proton chemical shifts along the other dimension. A spectral correlation function enables sorting of these strips according to proximity of the corresponding residues in the amino acid sequence. Thereby, starting from a given strip in the spectrum, the probability of its corresponding to the C-terminal neighboring residue is calculated for all other strips from the similarity of their peak patterns with a pattern predicted for the sequentially adjoining residue, as manifested in the scalar product of the vectors representing the predicted and measured peak patterns. Tests with five different proteins containing both -helices and -sheets, and ranging in size from 58 to 165 amino acid residues show that the discrimination achieved between the sequentially neighboring residue and all other residues compares well with that obtained with an unguided interactive search of pairs of sequentially neighboring strips, with important savings in the time needed for complete analysis of 3D 15N-correlated [1H, 1H]-NOESY spectra. The integration of this routine into the program package XEASY ensures that remaining ambiguities can be resolved by visual inspection of the strips, combined with reference to the amino acid sequence and information on spin-system types obtained from additional NMR spectra.Abbreviations 1D, 2D, 3D, 4D one-, two-, three-, four-dimensional - NOE nuclear Overhauser enhancement - NOESY nuclear Overhauser enhancement spectroscopy - COSY correlation spectroscopy - TOCSY total correlation spectroscopy  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号