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981.
Riding the sulfur cycle--metabolism of sulfonates and sulfate esters in gram-negative bacteria 总被引:1,自引:0,他引:1
Kertesz MA 《FEMS microbiology reviews》2000,24(2):135-175
Sulfonates and sulfate esters are widespread in nature, and make up over 95% of the sulfur content of most aerobic soils. Many microorganisms can use sulfonates and sulfate esters as a source of sulfur for growth, even when they are unable to metabolize the carbon skeleton of the compounds. In these organisms, expression of sulfatases and sulfonatases is repressed in the presence of sulfate, in a process mediated by the LysR-type regulator protein CysB, and the corresponding genes therefore constitute an extension of the cys regulon. Additional regulator proteins required for sulfonate desulfonation have been identified in Escherichia coli (the Cbl protein) and Pseudomonas putida (the AsfR protein). Desulfonation of aromatic and aliphatic sulfonates as sulfur sources by aerobic bacteria is oxygen-dependent, carried out by the alpha-ketoglutarate-dependent taurine dioxygenase, or by one of several FMNH(2)-dependent monooxygenases. Desulfurization of condensed thiophenes is also FMNH(2)-dependent, both in the rhodococci and in two Gram-negative species. Bacterial utilization of aromatic sulfate esters is catalyzed by arylsulfatases, most of which are related to human lysosomal sulfatases and contain an active-site formylglycine group that is generated post-translationally. Sulfate-regulated alkylsulfatases, by contrast, are less well characterized. Our increasing knowledge of the sulfur-regulated metabolism of organosulfur compounds suggests applications in practical fields such as biodesulfurization, bioremediation, and optimization of crop sulfur nutrition. 相似文献
982.
Isolation and characterisation of ethoprophos-degrading bacteria 总被引:1,自引:0,他引:1
An enrichment culture technique was used to isolate bacteria responsible for the enhanced biodegradation of ethoprophos in a soil from Northern Greece. Restriction fragment length polymorphism patterns of the 16S rRNA gene, partial 16S rRNA sequence analysis, and sodium dodecylsulfate-polyacrylamide gel electrophoresis total protein profile analysis were used to characterise the isolated bacteria. Two of the three ethoprophos-degrading cultures were pure and both isolates were classified as strains of Pseudomonas putida (epI and epII). The third culture comprised three distinct components, a strain identical to P. putida epI and two strains with 16S rRNA sequence similarity to Enterobacter strains. Isolate epI effectively removed a fresh ethoprophos addition from both fumigated and non-fumigated soil when introduced at high inoculum density, but removed it only from fumigated soil at low inoculum density. Isolates epI and epII degraded cadusafos, isazofos, isofenphos and fenamiphos, but only at a slow rate. This high substrate specificity was attributed to minor (cadusafos), or major (isazofos, isofenphos, fenamiphos) structural differences from ethoprophos. Studies with (14)C-labelled ethoprophos indicated that isolates epI and epII degraded the nematicide by removing the S-propyl moiety. 相似文献
983.
Amyloidosis is characterized by extracellular deposits of protein fibrils with a high content of β-sheets in secondary structure. The protein forms together with proteoglycans amyloid fibrils causing organ damage and serious morbidity. Intact apolipoprotein A-I (apoA-I) is an important protein in lipid metabolism regulating the synthesis and catabolism of high density lipoproteins (HDL). Usually, apoA-I is not associated with amyloidosis. However, four naturally occuring mutant forms of apoA-I are known so far resulting in amyloidosis. The most important feature of all variants is the very similar formation of N-terminal fragments which were found in the amyloid deposits (residues 1–83 to 1–94). The new insights in the understanding of the association of apoA-I with HDL, its metabolism, and its hypothesized structural findings may explain a common mechanism for the genesis of apoA-I induced amyloidosis. Here we summarized the specific features of all known amyloidogenic variants of apoA-I and speculate about its metabolic pathway, which may have general implications for the metabolism of apoA-I. 相似文献
984.
Seasonal and longitudinal metabolic trends in benthic communities were studied in the five orders of a natural mountain river
(the Stradomka) in southern Poland. The method of oxygen change of rocky substrata in chambers was applied. Metabolic data
were compared with abiotic (light regime, channel geomorphology and stream hydrology), and water chemical variables. A tendency
of downstream increase in gross production and community respiration was observed between 1st and 4th stream orders. The highest
values of GP (0.94 g C m-2 24 h -1) and CR (1.12 g C m-2 24 h -1) were observed in the middle Stradomka course (3–4 orders) in spring. A distinct negative NDM (heterotrophy) of the benthic
community was measured in autumn and spring. During winter NDM was almost constant and oscillated around zero. The P/R ratio
changed according to season and fluctuated between heterotrophy (P/R < 1) and autotrophy (P/R > 1). In general, heterotrophy
prevailed in the study system (except 5th order). A marked shift in P/R between 4th and 5th stream orders confirms the prediction
included in the RCC. Water temperature and PAR accounted for 56% in GP variability, whereas CR and NDM were not related to
these variables. The combined variables (PAR and PO4–P) attributed to 69% in GP variability. The set of chemical factors was less related to Stradomka metabolic data. These results
suggest that metabolic processes in this mountain river remarkably depend on physical variables (solar radiation, altitude,
water temperature and conductivity). Metabolic parameters were similar to other geographical mountain systems.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
985.
The observed endogenous circadian rhythm in plants performing Crassulacean acid metabolism is effected by malate transport
at the tonoplast membrane. Experimental and theoretical work asks for a hysteresis switch, regulating this transport via the
ordering state of the membrane. We apply a schematic molecular model to calculate the thermally averaged order parameter of
the membrane lipid structure in its dependence on external parameters temperature and area per molecule. The model shows a
first order structural phase transition in a biologically relevant temperature range. Osmotic consequences of malate accumulation
can trigger a transition between the two phases by changing the surface area of the cell vacuole. Estimation of the energy
needed to expand the vacuole under turgor pressure because of osmotic changes while acidifying shows that energy needed as
latent heat for the calculated change between phases can easily be afforded by the cell. Thus, malate content and the coexisting
two phases of lipid order, showing hysteretic behavior, can serve as a feedback system in an oscillatory model of Crassulacean
acid metabolism, establishing the circadian clock needed for endogenous rhythmicity.
Received: 2 September 1997/Revised: 24 April 1998 相似文献
986.
Effect of N source during soybean pod filling on nitrogen and sulfur assimilation and remobilization
During pod filling, a grain legume remobilizes vegetative nitrogen and sulfur to its developing fruit. This study was conducted to determine whether different nitrogen sources affected N and S assimilation and remobilization during pod filling. Well-nodulated plants fed 1.0 mM KNO3, 0.5 mM urea, or 2.5 mM urea assimilated 0%, 37%, or 114% more N, respectively, and 25%, 46%, or 56% more S, respectively, than did the average non-nodulated control plant fed 5.0 mM KNO3. Thus, N source during pod filling greatly affected both N and S assimilation. Depending upon N source, plant N concentration during pod filling decreased from 2.96% to between 1.36% and 1.82%. Non-nodulated control plants fed 5.0 mM KNO3 had the highest residual N at harvest. During the same treatments, plant S concentration decreased from 0.246% to a relatively uniform 0.215%. Thus, during pod filling, vegetative N was seemingly remobilized more efficiently (38–54%) than was S (13%). N source also affected seed yield and seed quality. Non-nodulated control plants fed 5.0 mM KNO3 produced the lowest yield (21.1 g seeds plant-1), whereas well nodulated plants fed 1.0 mM KNO3, 0.5 mM urea, or 2.5 mM urea produced yields of 26.2 g, 31.8 g, and 36.7 g seeds plant-1, respectively. Non-nodulated plants fed 2.5 mM urea yielded 28.6 g of seeds plant-1. Seed N concentrations of non-nodulated plants and nodulated plants fed 2.5 mM urea were high, 6.30% and 6.11% N, respectively, whereas their seed S concentrations were low, 0.348% and 0.330% S, respectively. N sources that produced both a relatively high seed yield and seed N concentration (i.e., a relatively high total seed N plant-1) produced a proportionately smaller increase in total seed sulfur. Consequently, seed quality, as judged solely by seed S concentration, was lowered. 相似文献
987.
988.
Effects of 2-Deoxy-d-Glucose on Methamphetamine-Induced Dopamine and Serotonin Neurotoxicity 总被引:1,自引:0,他引:1
Brian Callahan Jie Yuan Gretchen Stover George Hatzidimitriou George Ricaurte 《Journal of neurochemistry》1998,70(1):190-197
Abstract: The mechanisms underlying the neurotoxic actions of methamphetamine (METH) and related substituted amphetamines are unknown. Previous studies with 2-deoxyglucose (2-DG) have suggested that METH-induced neurotoxicity may involve exhaustion of intracellular energy stores. However, because 2-DG also produces hypothermic effects, and because METH's neurotoxic actions are highly susceptible to thermoregulatory influence, previous findings with 2-DG are difficult to interpret. The present studies were undertaken to further examine the influence of 2-DG's glucoprivic and thermic effects in the context of METH-induced dopamine (DA) and serotonin (5-HT) neurotoxicity. 2-DG protected against METH-induced DA neurotoxicity in both rats and mice. In both species, 2-DG, alone or in combination with METH, produced hypothermic effects. METH's toxic effects on brain 5-HT neurons were either unaffected or exacerbated by 2-DG, depending on species, brain region, and dose of METH tested. These results indicate that different mechanisms may underlie METH-induced DA and 5-HT neurotoxicity, and suggest that, as compared with 5-HT neurons, DA neurons are more susceptible to temperature influence, whereas 5-HT neurons are more vulnerable than DA neurons to metabolic compromise. Additional studies are needed to further assess the role of energy stores in the neurotoxic effects of METH and related drugs. 相似文献
989.
More than a 600-fold variation in nitrogen dioxide assimilation among 217 plant taxa 总被引:4,自引:0,他引:4
H. Morikawa A. Higaki M. Nohno M. Takahashi M. Kamada M. Nakata G. Toyohara Y. Okamura K. Matsui S. Kitani K. Fujita K. Irifune & N. Goshima 《Plant, cell & environment》1998,21(2):180-190
Assimilation of nitrogen dioxide in response to fumigation with 15 N-labelled nitrogen dioxide was studied in 217 plant taxa. The taxa included 50 wild herbaceous plants collected from roadsides (42 genera, 15 families), 60 cultivated herbaceous plants (55 genera, 30 families) and 107 cultivated woody plants (74 genera, 45 families). Two parameters, the 'NO2 -N content', or NO2 -derived reduced nitrogen content in fumigated plant leaves (mg N g–1 dry weight), and the 'NO2 -utilization index', or percentage of the NO2 -derived reduced nitrogen in the total reduced nitrogen, were determined. The NO2 -N content differed 657-fold between the highest ( Eucalyptus viminalis ; 6·57) and lowest ( Tillandsia ionantha and T. caput-medusae ; 0·01) values in the 217 taxa; 62-fold in a family (Theaceae) and 26-fold in a species ( Solidago altissima ). Nine species had NO2 -utilization indices greater than 10%, of which Magnolia kobus , Eucalyptus viminalis , Populus nigra , Nicotiana tabacum and Erechtites hieracifolia had NO2 -N contents > 4·9. These plants can be considered 'NO2 -philic' because in them NO2 -nitrogen has an important function(s). The Compositae and Myrtaceae had high values for both parameters, whereas the monocots and gymnosperms had low ones. These findings suggest that the metabolic pathway of NO2 -nitrogen differs among plant species. The information presented here will be useful for creating a novel vegetation technology to reduce the atmospheric concentration of nitrogen dioxide. 相似文献
990.
Omar SA 《Biodegradation》1998,9(5):327-336
Thirteen fungal species isolated from soil treated with pesticides were tested for their ability to mineralize and degrade three organophosphate insecticides currently used in Egypt (Cyolan®, Malathion® and Dursban®) in liquid media free from phosporus (P) and sulfur (S). All fungal species grew successfully on the culture media treated with the three used doses of insecticides (10, 50 and 100 ppm active ingredient) but the growth rate varied with the species, the insecticide and the doses. At 10 ppm level, insecticide degradation expressed in term of organic P mineralization (calculated as % of applied P) was the highest with all fungi tested. Organic P mineralization from pesticides was decreased by increasing the dose used to 50 and 100 ppm. The highest amount of P mineralized was observed with Cyolan® followed by Malathion® whilst P mineralization from Dursban® proceeded very slowly. Aspergillus terreus showed the greatest potential to mineralize organic P followed by A. tamarii, A. niger, Trichoderma harzianum and Penicillium brevicompactum whilst the remaining fungi only moderately mineralized the organic P component of the insecticides tested. Organic sulfur mineralization by the used fungal species paralleled, to some extent, organic P mineralization. The extracellular protein content of culture filtrates in the presence of various doses of insecticides was also decreased by increasing insecticide concentrations. The extracellular protein was significantly correlated with P and S mineralization (r = 0.89** and 0.64**, respectively) whilst correlation with cell dry mass was not significant (r = 0.03 and 0.003) suggesting a direct relationship between pesticide degradation and microbial protein production. The addition of P or S to the growth media enhanced extracellular protein excretion, and increased organic P and S mineralization by the most potent species tested (A. niger, A. tamarii, A. terreus and T. harzianum). This increment was significant in most cases, especially at the higher application rates. The relationship between extracellular protein excretion and organic P and S mineralization from insecticides was highly significant with the addition of inorganic phosphorus (r = 0.96** and 0.83**, respectively) or sulfur (r = 0.85** and 0.89**, respectively) to the growth media. 相似文献