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971.
One transgenic rice line lacking CrylAb expression product was screened in the progenies of Agrobacterium-transformed transgenic rice variety Zhong 8215 with a cry1Ab gene under field releasing conditions by using GUS histochemical assay and Western blot. Molecular hybridization results revealed that the crylAb gene was silenced in the transgenic rice variety Zhong 8215 and two copies of ubiquitin promoter were integrated into the rice genome. The silencing of crylAb gene in transgenic rice was found to be due to the methylation of the ubiquitin promoter as revealed by methylation analysis. Meanwhile, different concentrations of demethylation reagent 5-azacytidine combining with different treatment time were employed to treat the silenced transgenic rice seeds. The results indicated that 5-azacytidine could reactivate 8%-30% of the silenced transgenic rice plants and the expression level of the reactivated cry1Ab transgene could reach as high as 0.147% of the total soluble protein. Treatment with low con 相似文献
972.
Zheng Gui-Zhao Yang Yue-Sheng Chen Xiong-Hui Wan Bang-Hui 《Plant Cell, Tissue and Organ Culture》2002,68(2):195-202
A newly established culture method was evaluated for application in mass-propagation of a photoperiod-temperature sensitive genic male sterile rice strain. It was found that the new culture method was very efficient for the regeneration of plants from callus of this strain, and a high concentration of 6-benzyladenine necessary for highly efficient plant regeneration culture did not seem to have negative effects on some agronomic traits investigated of the regenerated plants and their progenies. By the new culture method, a large number of plants could be regenerated in a relatively short time (approximately 8×1012 plants within 300 days) from one naked seed-explant. Because of this, prominent drift of the PTGMS rice strain, which accumulates gradually during the course of propagation of the seeds through generations, in the response to photoperiod-temperature conditions could be avoided when this in vitro culture protocol is employed instead of seed propagation. Differences in rates of pollen fertility and seed setting were found between regenerated plants and seed-grown plants, and between their respective first and second progenies. These differences are also of important application value in two-line rice production: Higher pollen fertility of the regenerated plants can be applied with higher efficiency for the propagation of the strain seeds because they have much higher seed setting by self-fertilization; lower pollen fertility of the regenerated plant progenies will result in much lower seed setting by self-fertilization and is, therefore, conducive to the production of hybrid seeds of higher purity by natural crossing with the other line in the two-line system. 相似文献
973.
974.
975.
976.
中国普通野生稻遗传分化的RAPD研究 总被引:18,自引:0,他引:18
多数学者已认定亚洲栽培稻(OryzasativaL.)的祖先是普通野生稻(O.rufipogon)。然而栽培稻的籼、粳分化是发生在驯化之前还是在驯化之后,也即普通野生稻是否存在籼、粳分化的问题,是十几年来稻作起源研究中争论的热点之一。Second[1]用多个同工酶位点的分析结果得出结论,普通野生稻在驯化为栽培稻之前就已经发生了籼、粳分化,即有籼型普通野生稻和粳型普通野生稻之分。Morishima和Gadrinab[2]用24个形态和生理性状及12个同工酶位点和杂交亲合力等方法证明普通野生稻没有发… 相似文献
977.
不同灌溉模式对稻田土壤及糙米重金属积累的影响 总被引:1,自引:0,他引:1
为探讨降低土壤和糙米重金属的灌溉模式, 选取江西省萍乡地区的中度重金属污染稻田, 研究了灌浆期习惯性灌溉(间歇性灌溉)和长期淹水灌溉2种灌溉模式对水稻产量、糙米重金属及土壤理化性质的影响。结果表明: 与习惯性灌溉相比, 长期淹水灌溉条件下的水稻产量降低1.25%; 糙米Cd和Pb含量分别降低42.79%和3.70%, 其中糙米Cr含量显著降低44.81%, 但糙米Hg含量显著增加200%, 糙米无机As含量增加3.29%; 土壤Cd含量降低38.77%, 其中土壤有效态Cd含量和阳离子交换量显著降低72.08%和36.71%, 但土壤pH和有机质增加0.97%和6.32%; 同时相关性分析发现糙米Cr含量与土壤有机质呈显著负相关, 与阳离子交换量呈显著正相关, 糙米Hg含量与土壤有效态Cd含量和阳离子交换量呈极显著负相关。长期淹水灌溉可有效降低污染稻田土壤Cd进入水稻, 实现水稻安全生产, 但会增加糙米Hg积累和减产。 相似文献
978.
水稻条纹叶枯病抗性位点的检测和效应分析 总被引:2,自引:0,他引:2
利用111个家系组成的热研2号(Oryza sativa subsp.japonica‘Reyan2’)/Milyang23(Oryza sativa subsp.indica‘Milyang23’)重组自交系(recombinant inbred lines,RIL)群体(F7),采用重病区田间自然接种方法,以病情指数作为条纹叶枯病的表型值,鉴定了2个亲本及111个RIL家系对条纹叶枯病的抗性。使用QTL Cartographer软件复合区间作图法,对水稻(Oryza sativa)条纹叶枯病抗性基因进行了QTL分析。结果检测到2个抗水稻条纹叶枯病的QTL,分别位于第2和第11染色体上,其中第11染色体上的QTL贡献率为19.58%,表明这是一个主效的QTL,该QTL及其附近的分子标记,可以用于水稻条纹叶枯病抗性分子标记辅助育种。 相似文献
979.
Gravitropism of maize and rice coleoptiles was investigated with respect to its dependence on the angle of displacement or the initial stimulation angle (ISA). Close examination of curvature kinetics and the response to a drop in stimulation angle (SA) indicated that the gravtropic response during an early but substantial part of the curvature development is directly related to the ISA, there being no effect of the reduction of SA resulting from the curvature response itself. On the basis of this finding, the relationship between the steady SA and the curvature rate was determined. In maize, the curvature rate increased linearly with the sines of SAs up to an SA of 90 degrees. Rice coleoptiles, however, showed a saturation curve in the same range of SAs. The saturation profile was nearly identical between coleoptiles grown in air and those submerged in water, although the latter elongated much faster. Rice coleoptiles appeared to be far more sensitive to gravity than maize coleoptiles. It is concluded that the sensitivity to gravity, assessed through dependence on ISA, is a property inherent to a given gravitropic organ. Long-term measurements of curvature indicated that the coleoptiles bend back past the vertical. This overshooting was marked in submerged rice coleoptiles. 相似文献
980.