Modeling and mechanism of the adsorption of proton onto natural bamboo sawdust

Natural bamboo sawdust with cellulose-lignin polymeric structure was used as a raw adsorbent to remove heavy metal and dyes in water. The analysis of surface properties showed that high proton affinity sites were mainly composed of phenolic and alcohol hydroxyl, while low proton affinity sites mainly consisted of carboxylic acid, silicon hydroxyl, aluminum hydroxyl and some low densities of sulfhydryl and phosphoryl groups. The results fitted by NEM surface complexation model showed: three-sites model fitted acid-base titration data better than two-sites and one-site model. Acid-base titration experiments showed the amount of acid and base consumed was not uniform between acid and alkali side. This was probably due to the swelling of cellulose in acid side, but the possibility that different reactions occurred in acid and alkali side cannot be completely excluded. Pznpc of sawdust was between pH 5.2 and 5.5.     

Escherichia coli expression and refolding of E/K-coil-tagged EGF generates fully bioactive EGF for diverse applications

Heterodimerizing peptides, such as the de novo designed E5/K5 peptide pair, have several applications including as tags for protein purification or immobilization. Recently, we demonstrated that E5-tagged epidermal growth factor (EGF), when bound to a K4 expressing adenovirus, promotes retargeting of the adenovirus to EGFR expressing target cells. In this study, we present the Escherichia coli expression, refolding and purification of human EGF fused with the E5-coil (E5-coil-EGF) or with the K5-coil (K5-coil-EGF). EGF receptor phosphorylation and cell proliferation assays demonstrated that the biological activity of the coil-tagged EGF versions was comparable to that of non-tagged EGF. Additionally, analysis of the binding of E5/K5-coil-EGF to cell surface EGFR or to soluble EGFR ectodomain, as measured by cell-based binding competition assays and by SPR-based biosensor experiments, indicated that the coil-tagged EGF versions bound to EGFR with affinities similar to that of non-tagged EGF. Finally, we show that E-coil-tagged EGF, but not non-tagged EGF, can retarget a K-coil containing adenovirus to EGF receptor expressing glioblastoma tumor cells. Overall these results indicate that E. coli expression offers a practical platform for the reproducible production of fully biologically active E5/K5-coil-tagged EGF, and support applications of heterodimerizing coil-tagged ligands, e.g. the targeting of viruses or other entities such as nanoparticles to tumor cells, or growth factor immobilization on cell culture scaffolds for tissue engineering.     

Likely effects of salinity on acute copper toxicity to the fisheries of the lake George-Edward basin

Alkaline, saline waters are common in the Western Rift Valley of East Africa, in which the lake George-Edward basin is situated. A growing copper mining industry in the area makes it important to understand the limnology of the lakes in this basin before copper pollution occurs. The fish could possibly suffer from acute (or chronic) toxicity if copper levels increase.Abiotic factors within the alkaline, saline waters of this basin reduce the acute toxic effects of copper to fish. The most important factor is salinity, which is a measure of the total dissolved mineral salts. The relatively highly concentrations of mineral salts of these waters will to reduce the effective copper ionic activity through adsorption, precipitation, and ionic interference. The high concentrations of organic compounds in the waters, also complex and chelate the ionic Cu²⁺, thus reducing further its effective concentration. This will therefore act as a check on the copper toxicity to the fish of the lake basin.