全文获取类型
收费全文 | 195篇 |
免费 | 8篇 |
国内免费 | 9篇 |
出版年
2024年 | 1篇 |
2020年 | 12篇 |
2019年 | 5篇 |
2018年 | 5篇 |
2017年 | 7篇 |
2016年 | 7篇 |
2015年 | 2篇 |
2014年 | 7篇 |
2013年 | 14篇 |
2012年 | 6篇 |
2011年 | 12篇 |
2010年 | 11篇 |
2009年 | 11篇 |
2008年 | 21篇 |
2007年 | 12篇 |
2006年 | 14篇 |
2005年 | 6篇 |
2004年 | 14篇 |
2003年 | 12篇 |
2002年 | 3篇 |
2001年 | 3篇 |
2000年 | 1篇 |
1999年 | 4篇 |
1998年 | 1篇 |
1997年 | 5篇 |
1996年 | 1篇 |
1994年 | 1篇 |
1993年 | 1篇 |
1992年 | 1篇 |
1991年 | 1篇 |
1990年 | 2篇 |
1989年 | 2篇 |
1988年 | 1篇 |
1987年 | 2篇 |
1985年 | 2篇 |
1980年 | 1篇 |
1976年 | 1篇 |
排序方式: 共有212条查询结果,搜索用时 31 毫秒
61.
Lei Cao-Lei Kelsey N Dancause Guillaume Elgbeili Renaud Massart Moshe Szyf Aihua Liu David P Laplante Suzanne King 《Epigenetics》2015,10(8):749-761
Prenatal maternal stress (PNMS) in animals and humans predicts obesity and metabolic dysfunction in the offspring. Epigenetic modification of gene function is considered one possible mechanism by which PNMS results in poor outcomes in offspring. Our goal was to determine the role of maternal objective exposure and subjective distress on child BMI and central adiposity at 13½ years of age, and to test the hypothesis that DNA methylation mediates the effect of PNMS on growth. Mothers were pregnant during the January 1998 Quebec ice storm. We assessed their objective exposure and subjective distress in June 1998. At age 13½ their children were weighed and measured (n = 66); a subsample provided blood samples for epigenetic studies (n = 31). Objective and subjective PNMS correlated with central adiposity (waist-to-height ratio); only objective PNMS predicted body mass index (BMI). Bootstrapping analyses showed that the methylation level of genes from established Type-1 and -2 diabetes mellitus pathways showed significant mediation of the effect of objective PNMS on both central adiposity and BMI. However, the negative mediating effects indicate that, although greater objective PNMS predicts greater BMI and adiposity, this effect is dampened by the effects of objective PNMS on DNA methylation, suggesting a protective role of the selected genes from Type-1 and -2 diabetes mellitus pathways. We provide data supporting that DNA methylation is a potential mechanism involved in the long-term adaptation and programming of the genome in response to early adverse environmental factors. 相似文献
62.
Anastassopoulos E 《Cryobiology》2006,53(2):276-278
An agar plate freezing assay is described based on the incorporation of fluorescein dye in agar medium. Upon addition of fluorescein the medium becomes transparent. This facilitates the monitoring of the ice nucleation event in vivo and the subsequent in situ selection of transformed ice nucleating bacteria. In comparison with known assays for the screening of transformants, the proposed assay is very accurate and reproducible. It may be applied in environmental samples screening for ice nucleating organisms, or in cDNA or genomic libraries for identifying novel ice nucleation genes. It may also prove useful in comparative studies of the ice nucleation activity, e.g. in directed evolution experiments involving ice nucleation genes. 相似文献
63.
The basis for hyperactivity of antifreeze proteins 总被引:4,自引:0,他引:4
Antifreeze proteins (AFPs) bind to the surface of ice crystals and lower the non-equilibrium freezing temperature of the icy solution below its melting point. We have recently reported the discovery of three novel hyperactive AFPs from a bacterium, a primitive insect and a fish, which, like two hyperactive AFPs previously recognized in beetles and moths, are considerably better at depressing the freezing point than most fish AFPs. When cooled below the non-equilibrium freezing temperature, ice crystals formed in the presence of any of five distinct, moderately active fish AFPs grow suddenly along the c-axis. Ice crystals formed in the presence of any of the five evolutionarily and structurally distinct hyperactive AFPs remain stable to lower temperatures, and then grow explosively in a direction normal to the c-axis when cooled below the freezing temperature. We argue that this one consistent distinction in the behaviour of these two classes of AFPs is the key to hyperactivity. Whereas both AFP classes bind irreversibly to ice, the hyperactive AFPs are better at preventing ice growth out of the basal planes. 相似文献
64.
Using the ice algal community growing at the bottom of the annual sea ice in McMurdo Sound Antarctica, the response of the
photoprotective diadinoxanthin (DD)-cycle to exposure to light was investigated. Changes in pigment concentration were detected
using high-performance liquid chromatography. A light mixing simulator (LMS) was developed and used to simulate the pigment
response to mixing in the upper water column. No DD-cycle was detected under the sea ice under natural light conditions. The
DD-cycle was activated after exposure to surface natural light conditions and artificial light conditions. The first-order
kinetic rates of the DD-cycle under constant artificial irradiance, natural irradiance and simulations with the LMS were found
to be similar to other studies suggesting that ice algae do not vary the rate of deepoxidation depending on light history.
Simulations under natural light using the LMS demonstrated that the response of the DD-cycle to static incubations and when
subject to vertical mixing was not similar, and that static incubations overestimate DD-cycle activity over the long term.
Algae in a simulated vertically mixed environment were able to increase the pool of xanthophyll pigments compared to static
conditions where the pool remained the same or decreased. The recovery of DD in the dark or under low light was found to be
significantly faster than in temperate algal communities. These results suggest that ice algae at the sea ice bottom can activate
the photoprotective DD-cycle to regulate excess thermal energy. Unlike temperate species of diatoms, ice algae can rapidly
reconstruct the pigment pool under low light or in the dark and is likely a particular adaptation to the unique light environment
in Antarctica. 相似文献
65.
The ice crystal formation is assumed as the most lethal factor for the failure of fish embryo cryopreservation and intracellular ice formation (IIF) plays a central role in cell injury during cooling. The objectives were to observe the morphological changes of red seabream (Pagrus major) embryo during the cooling–thawing process, and to examine the effect of cryoprotectant and cooling rate on the temperatures of oil globule ice formation (TOIF), extra-cellular ice formation (TEIF) and intracellular ice formation (TIIF) using cryomicroscope. After thawing, the morphological changes of embryos were observed and recorded by the video attachment and monitor under the microscope. During the cooling process, three representative phenomena were observed: oil globule gradually turned bright firstly, then the whole field of view flashed and the embryo blackened. Cooling rate affect the temperature of both extra- and intra-cellular ice formations. TEIF and TIIF at high cooling rate were much lower than that at low cooling rate. And the value of TEIF − TIIF increased from 0.45 to 11.11 °C with the increase of cooling rate from 3 to130 °C/min. Taken together, our results suggested that high cooling rate with proper cryoprotectant would be a good option for red seabream embryo cryopreservation. 相似文献
66.
The effects of cryopreservation on tendon allograft have been reported, but remain unclear, particularly the potential effects
on mechanical properties and histological changes by ice crystal formation. There are also few studies about effects of heating
for sterilization of tendon. We evaluated the effect of cryopreservation or heating on the mechanical properties and histomorphology
of rat bone-patellar tendon-bones (BTBs). BTBs were processed by cryopreservation at −80°C for 3 weeks, or heating at 80°C
for 10 min. Tensile testing and histomorphological examination were performed. The cryopreservation of tendons showed less
influences on their mechanical properties. When cryopreserved BTBs in frozen state were fixed by freeze-substitution method,
many spaces were observed in interfibrillar substances. These results suggest that the collagen fibers of cryopreserved tendons
were histomorphologically affected by ice crystals. The heating of tendons completely destroyed the collagen fibers of the
tendons and is therefore thought to be inappropriate for the sterilization of BTBs. 相似文献
67.
甲虫抗冻蛋白是一种具有规则结构的昆虫抗冻蛋白。在相同浓度条件下,甲虫抗冻蛋白比鱼类抗冻蛋白有更高的热滞活性,目前已成为人们重点研究的一类抗冻蛋白。根据甲虫抗冻蛋白的结构特点及其在冰晶表面的吸附模式,应用二维吸附结合模型计算分析了具有6 ̄11个β-螺旋(β-helix)结构片段的甲虫抗冻蛋白变体分子,得到了它们的热滞活性随溶液浓度变化的规律,特别是热滞活性与甲虫抗冻蛋白的β-螺旋结构片段数的关系。结果显示,抗冻蛋白在冰晶表面的覆盖度是一个影响其热滞活性的重要因素。 相似文献
68.
Janet A.W. Elliott 《Cryobiology》2010,60(1):1-3
This brief paper introduces the subject of thermodynamics and the papers of the special issue on thermodynamic aspects of cryobiology. Thermodynamic terminology is defined for the non-specialist. 相似文献
69.
T.M. Cronin K. Hayo G.S. Dwyer D.A. Willard 《Palaeogeography, Palaeoclimatology, Palaeoecology》2010,297(2):299-310
A new 2400-year paleoclimate reconstruction from Chesapeake Bay (CB) (eastern US) was compared to other paleoclimate records in the North Atlantic region to evaluate climate variability during the Medieval Climate Anomaly (MCA) and Little Ice Age (LIA). Using Mg/Ca ratios from ostracodes and oxygen isotopes from benthic foraminifera as proxies for temperature and precipitation-driven estuarine hydrography, results show that warmest temperatures in CB reached 16-17 °C between 600 and 950 CE (Common Era), centuries before the classic European Medieval Warm Period (950-1100 CE) and peak warming in the Nordic Seas (1000-1400 CE). A series of centennial warm/cool cycles began about 1000 CE with temperature minima of ~ 8 to 9 °C about 1150, 1350, and 1650-1800 CE, and intervening warm periods (14-15 °C) centered at 1200, 1400, 1500 and 1600 CE. Precipitation variability in the eastern US included multiple dry intervals from 600 to 1200 CE, which contrasts with wet medieval conditions in the Caribbean. The eastern US experienced a wet LIA between 1650 and 1800 CE when the Caribbean was relatively dry. Comparison of the CB record with other records shows that the MCA and LIA were characterized by regionally asynchronous warming and complex spatial patterns of precipitation, possibly related to ocean-atmosphere processes. 相似文献
70.
Collapse of the West Antarctic Ice Sheet (WAIS) would raise global sea level by ~3.3–5 m. Ice‐sheet models and geological data suggest at least one collapse has happened during the last 1.1 Ma, and some scenarios of future climate change predict a collapse within the next two centuries. A complete WAIS collapse would open shallow seaways across West Antarctica, potentially enabling exchange of animals between West Antarctic seas. We investigated biological evidence for past connectivity between different regions of Antarctica by comparing the composition of modern bryozoan assemblages from the continental margin around Antarctica. Surprisingly, we found most similarity between two areas which are not currently connected – the shelves of the Weddell Sea (WS) and Ross Sea (RS). We evaluated three hypotheses to explain this and conclude that bryozoans most likely dispersed through a trans‐Antarctic seaway that opened in response to a WAIS collapse and connected the WS and RS shelves. These bryozoans must have survived glaciations(s) during subsequent ice ages in refuges, whereas they were wiped out in most other regions of the Antarctic shelf. After the last glacial period, bryozoan assemblages could freely disperse between many of the regions we examined (e.g. Antarctic Peninsula and South Shetland Islands), which has allowed recolonization of areas in which bryozoans had been eradicated during the last ice age. For the bryozoans on the WS and RS shelves to be more similar than those which are in close proximity means the trans‐Antarctic seaway may have been as late as the last few interglacials. Current rates of warming are exceptional compared with the near past glacial cycles so our study, the strongest faunal evidence of WAIS collapse during the recent geological past, thus supports predictions of a near future WAIS collapse (with considerable global sea level implications) and resultant future major faunal exchanges. 相似文献