伊贝母体细胞无性系的建立及其胚状体的发生

本文报道了伊贝母体细胞无性系的建立及其胚状体的发生。已继代培养三年零六个月共30多代的鳞芽愈伤组织,目前仍有分化能力。通过愈伤组织形态细胞学的观察,发现伊贝母体细胞无性系形成小鳞茎的途径有二:一是由特化了的愈伤组织表皮细胞。经多次分裂发育成不定芽而形成小鳞茎;二是由愈伤组织表层或内层特化了的胚性细胞,经多次分裂发育成胚状体而形成小鳞茎。不定芽和胚状体的形态发生是有区别的。     

用ST-EPR研究胆酸对F_1-F_0在F_1-F_0复合体中慢运动的影响

本文比较了经胆酸处理和对照样品的马未酰亚胺自旋标记的F_1-F_0酶复合体的ST-EPR波谱, 二者波谱呈现明显不同的形态.经胆酸处理的复台体中F_h-F_0酶蛋白的波谱参数L”/L’,C’/C,H”/H较对照样品明显增大.选用参数L”/L’计算其相关时间τ_c,得出对照的F_1-F_0酶蛋白的τ_e为5.5×10~(-5)秒而经胆酸处理的F_1-F_0酶蛋白的τ_e为1×10~(-4)秒.表明胆酸处理的样品中酶蛋白运动大大减慢.因此推测胆酸可能通过增加F_1-F_0酶蛋白在膜脂中的旋转运动相关时间,减慢其运动速率,来抑制Mg~2 对F_1-F_0酶水解活力的激活作用的.     

恒河猴B病毒相关抗体检测方法的比较

以NT方法为基础比较了ELISA和FIA方法,共检测84份猴B病毒相关抗体的敏感性,结果ELISA和EIA阳性各50份(59.5％),NT阳性45份(53.6％)。三种方法相符者71份,符合率84.5％。ELISA和FIA均较NT敏感,而且快速,简便,经济,可应用于大批标本的检查。     

人粪便中分离的噬菌蛭弧菌生物学特性的研究

本文研究了人粪便中分离的噬菌蛭弧菌的生物学特性,测定了它们的生长曲线,并利用微孔滤膜过滤和机械振荡的方法,研究了它们的吸附和穿入动力学,发现链霉素、庆大霉素和卡那霉素能抑制蛭弧菌的吸附;青霉素不影响蛭弧菌的吸附和穿入,但抑制蛭弧菌在宿主内的生长过程。从人粪中分出的噬菌蛭弧菌不仅能裂解大部分需氧的革兰氏阴性菌,而且在微氧条件下也能裂解厌氧菌中的二株脆弱拟杆菌。我们发现一株人粪便中分出的噬菌蛭弧菌能形成蛭弧菌囊体——它的休眠态,它对高热、紫外线照射和真空干燥的耐受力较相应的繁殖体强,看来它是蛭弧菌保持生命期限的一种方式。     

在被小泡参与突触前后膜特化增厚的形态学研究

Our object was to characterize the morphological changes of coated vesicles and synaptic membranes during synaptogenesis. Neurons from spinal cords of fetal mice were established as isolated cells in primary culture. After a few days in vitro, the neurons extended their neurites and started their interaction. At timed intervals thereafter, cultures were fixed for electron microscopic observation. Coated vesicles were prominent in the neuronal cytoplasm at the time of synaptogenesis (about 7-10 days in vitro). Similar vesicles were seen in continuity with some cisternae in the Golgi regions and there was an increase in number during the synaptogenic period. Indeed it is not established whether the coated vesicles were exocytotic or pinocytotic in nature, but the cisternae which were in continuity with coated vesicles could be labelled by glucose-6-phosphatase (G6Pase) but not by thiamine pyrophosphatase (TPPase). Such vesicles were also seen in continuity with the neuronal plasmalemma near the closest contact site and contributed their undercoating to pre- and postsynaptic densities. The formation of bilateral membrane specialization was described as being structurally similar to synaptic active zones and appeared to be the first definitive sign of synapseformation. It has been suggested that the synaptic dense material may derive wholly or in part from the exocytic coated vesicles which apparently budding off from endoplasmic reticulum cisternae. This incorporation could provide the mechanism for confining specific characteristics of neuronal membrane to the synaptic region.     

中国志留纪鱼类微体化石的首次报道

一、前言滇东曲靖晚志留世地层发育良好,层序清楚,无脊椎动物和脊椎动物化石以及牙形刺丰富,是研究我国志留系生物地层的一个重要地区。这套地层自下而上被命名为关底组、妙高组和玉龙寺组,是一套海陆交互相沉积,其海相地层为典型的壳相沉积,为一种近岸、浅水、富氧的沉积环境(丁文江、王日伦,1937;方润生、江能人等,1985)。本文记述的无颌类和鱼类化石就产自海相地层,它们与牙形刺和其他一些     

正常月经周期妇女卵泡液中激素水平的变化

本文测定了24例正常月经妇女在不同时相、不同大小卵泡的卵泡液中雌二醇(E_2)、孕酮(P_0)、雄烯二酮(A)、睾酮(T)、卵泡刺激素(FSH)、黄体生成素(LH)和催乳素(PRL)的含量,并分析其与外周血中相应激素浓度的关系。测定结果显示:小卵泡的卵泡液中E_2、Po,FSH,LH水平低于大卵泡中水平,而A和T水平则相反。排卵前大卵泡中E_2(9815nmol/L),P_0(3316nmol/L),FSH(1.34IU/L)和LH(3.9lIU/L)达最高值。A(280nmol/L)和T(137nmol/L)却较小卵泡中水平低(相应为692nmol/L和176nmol/L)。PRL水平在大小卵泡中无显著性差异。卵泡液中甾体激素水平高于外周血7—20.000倍,FSH、LH水平为外周血的10—80％,PRL水平为60％—3倍。     

组胺H2受体拮抗剂对骨髓造血重建的影响

用组胺H_2受体拮抗剂(甲氰咪胍或呋喃硝胺)处理正常和亚致死量γ-射线照射小鼠,探讨正常体内造血和再生骨髓中造血重建与组胺受体的关系。发现非毒性剂量的甲氰咪胍对正常小鼠骨髓多能造血干细胞(CFU-s)无抑制作用,但可抑制小鼠体内粒单系祖细胞(CFU-GM)的生长和亚致死量照射后CFU-s产率的恢复。组胺可能与骨髓的再生有关,组胺H_2受体拮抗剂可抑制骨髓的造血重建。