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181.
In addition to assessing the impacts of CO2 doubling on environment and society, more consideration is needed to estimate extreme events or surprises. This is particularly important at the intersection of disciplines like climate and ecology because the potential for large discontinuities is high given all the possible climate/biota interactions. The vast disparities in scales encountered by those working in traditional ecology (typically 20 m) and climatology (typically 200 km) make diagnoses of such interactions difficult, but these can be addressed by an emerging research paradigm we call strategic cyclical scaling (SCS). The need to anticipate outlier events and assign them subjective probabilities suggests emphasis on interdisciplinary research associations. The desire to reduce societal vulnerability to such events suggests the need to build adaptive management and diverse economic activities into social organizations. The effectiveness of adaptation responses to anticipated climatic changes is complicated when consideration of transient changes, regional disturbances, large unforseeable natural fluctuations and surprises are considered. Slowing down the rate of disturbances and decreasing vulnerability are advocated as the most prudent responses to the prospect of human-induced climatic changes. 相似文献
182.
Wolbachia are strictly endocellular, vertically transmitted bacteria associated with insects and crustaceans. This group of parasites modify their hosts' reproduction so as to increase their own fitness. This paper reviews the variability of these parasitic alterations and their consequences for host biology and populations. Wolbachia induce cytoplasmic incompatibility (a characteristic apparently specific to Wolbachia) in several insects and one isopod crustacean; parthenogenesis (thelytoky) in haplo-diploid insects; feminization in various isopods. The consequences of these phenomena on speciation, population dynamics and genetic polymorphism are discussed. The variability of the mechanisms of host sex determination is one important factor responsible for the diversity of Wolbachia-host interactions. However, parasite characteristics, such as the capacity to disturb host mitosis, and the ability to be horizontally transferred between hosts, also appear to play a role in this diversity. 相似文献
183.
Kinetic analysis of ligand binding to interleukin-2 receptor complexes created on an optical biosensor surface. 总被引:3,自引:2,他引:1
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D. G. Myszka P. R. Arulanantham T. Sana Z. Wu T. A. Morton T. L. Ciardelli 《Protein science : a publication of the Protein Society》1996,5(12):2468-2478
The interleukin-2 receptor (IL-2R) is composed of at least three cell surface subunits, IL-2R alpha, IL-2R beta, and IL-2R gamma c. On activated T-cells, the alpha- and beta-subunits exist as a preformed heterodimer that simultaneously captures the IL-2 ligand as the initial event in formation of the signaling complex. We used BIAcore to compare the binding of IL-2 to biosensor surfaces containing either the alpha-subunit, the beta-subunit, or both subunits together. The receptor ectodomains were immobilized in an oriented fashion on the dextran matrix through unique solvent-exposed thiols. Equilibrium analysis of the binding data established IL-2 dissociation constants for the individual alpha- and beta-subunits of 37 and 480 nM, respectively. Surfaces with both subunits immobilized, however, contained a receptor site of much higher affinity, suggesting the ligand was bound in a ternary complex with the alpha- and beta-subunits, similar to that reported for the pseudo-high-affinity receptor on cells. Because the binding responses had the additional complexity of being mass transport limited, obtaining accurate estimates for the kinetic rate constants required global fitting of the data sets from multiple surface densities of the receptors. A detailed kinetic analysis indicated that the higher-affinity binding sites detected on surfaces containing both alpha- and beta-subunits resulted from capture of IL-2 by a preformed complex of these subunits. Therefore, the biosensor analysis closely mimicked the recognition properties reported for these subunits on the cell surface, providing a convenient and powerful tool to assess the structure-function relationships of this and other multiple subunit receptor systems. 相似文献
184.
Michèle Thomas-Orillard 《Biodiversity and Conservation》1996,5(8):1015-1021
Drosophila melanogaster can be parasitized by a picornavirus, the Drosophila C virus (DCV). The virus is not hereditary, but it is horizontally transmitted (by ingestion or contact). When first larval instars come into contact with DCV unusual interactions are observed between host and microparasite. DCV acts differently depending on the stage in the host's life cycle. It boosts the reproductive capacity of adults, but it diminishes survival during the pre-reproductive period. In infected flies, the DCV target organs are principally the follicular cells and the fat body. The infected cells resemble DCV-free cells. According to the parameters of the Drosophila lifecycle, measured for different Drosophila strains, at different temperatures, and for different viral doses, DCV could be considered either as a parasite, because it increases pre-adult mortality, or as a mutualist, because it increases the reproductive capacity of the host and decreases its developmental time. Like many viruses, DCV is extremely pathogenic when injected into flies, which then die within a few days. Only one strain resists the disease longer. The resistant phenotype is dominant. Genes of chromosome 3 of the host are involved. Interactions are discussed in terms of an arms race and peaceful cohabitation. They are also considered in terms of biodiversity for the host and for the microparasite. 相似文献
185.
ANIL K. BHUNIA DWIJEN SARKAR PIJUSH K. DAS 《The Journal of eukaryotic microbiology》1996,43(5):373-379
We investigated activation signaling events in bone marrow-derived macrophages after infection with Leishmania donovani, an intracellular parasite of macrophages. Leishmania donovani infection caused a general suppression of activation parameters like O2- and NO production. However, conditions which allow parasite attachment and prevent entry resulted in triggering of O2- and NO production and stimulation of O2 consumption. Optimal NO and O2- production occurred when bone marrow-derived macrophages and Leishmania ratio was 1:100. The activation signal for O2- production was initiated 15 min after parasite attachment, whereas augmentation of NO production started 6 h after attachment. Activation of O2- and NO generation by L. donovani attachment was inhibited by staurosporine as well as by prolonged treatment of phorbol myristate acetate suggesting a protein kinase C-dependent mechanism. Translocation studies showed that protein kinase C activity in cell membrane fraction rapidly and transiently increased following parasite attachment. No such protein kinase C translocation event occurred in L. donovani infected bone marrow-derived macrophages. Phorbol myristate acetate was found to stimulate membrane translocation of protein kinase C in parasite attached cells whereas it was impaired in infected cells. However, both attachment and infection induced a similar shift of phorbol receptors from cytosolic to membrane fraction indicating that in infected cells the translocation of protein kinase C protein was not impaired but the activity of the membrane associated enzyme was somehow inhibited. These results suggest that although internalization of intracellular parasites like L. donovani caused inhibition of nitrite and superoxide release, mere attachment on macrophage surface resulted in an activation of protein kinase C-mediated downstream oxidative events. 相似文献
186.
W. B. Curry M. D. Grabe I. V. Kurnikov S. S. Skourtis D. N. Beratan J. J. Regan A. J. A. Aquino P. Beroza J. N. Onuchic 《Journal of bioenergetics and biomembranes》1995,27(3):285-293
The simplest views of long-range electron transfer utilize flat one-dimensional barrier tunneling models, neglecting structural details of the protein medium. The pathway model of protein electron transfer reintroduces structure by distinguishing between covalent bonds, hydrogen bonds, and van der Waals contacts. These three kinds of interactions in a tunneling pathway each have distinctive decay factors associated with them. The distribution and arrangement of these bonded and nonbonded contacts in a folded protein varies tremendously between structures, adding a richness to the tunneling problem that is absent in simpler views. We review the pathway model and the predictions that it makes for protein electron transfer rates in small proteins, docked proteins, and the photosynthetic reactions center. We also review the formulation of the protein electron transfer problem as an effective two-level system. New multi-pathway approaches and improved electronic Hamiltonians are described briefly as well. 相似文献
187.
188.
Plant stress and larval performance of a dipterous gall former 总被引:2,自引:0,他引:2
L. De Bruyn 《Oecologia》1995,101(4):461-466
According to the plant vigour hypothesis, galling insects should respond positively and perform better on vigorous plants or plant parts, the opposite of the predictions of the plant stress hypothesis. I carried out field experiments to analyse the effects of sustained abiotic stress on the interactions between the common reed (Phragmites australis) and a gall-forming fly (Lipara lucens). The reed shoot diameter (a measure of plant vigour) is strongly affected by environmental conditions, where dry and/or nutrient-poor habitats produce thinner (stressed) shoots. L. lucens gall density is negatively correlated with shoot diameter. In a survival experiment with a wide range of shoot diameters, larval mortality was also highly correlated with shoot quality. Gall formation was higher on thinner, stressed shoots. An analysis of the gall tissues revealed that galls induced by L. lucens contain a high amount of a nutrient-rich feeding tissue. The impact of L. lucens is higher on thinner shoots. The results of this study showed that L. lucens performs better on stressed hosts, which contradicts the plant vigour hypothesis for galling insects. The low nutrient availability in the stressed shoots can be compensated by the production of galls with a nutrient-rich feeding tissue. 相似文献
189.
We analysed the relative effects of food availability and temperature on rates of growth and development of a predatory planktonic
water mite, Piona exigua. Growth in length of mites fed Daphnia, Ceriodaphnia and Chydorus was analysed by Gompertz or von Bertalanffy curves; these curves were compared by parallel curve analysis.
Growth rates of nymphs and adult female mites increased with temperature; the duration of the imagochrysalis stage decreased.
Females grown at 10 °C were smaller at final size than females grown at 15 °C, 18 °C or 22 °C. Females reared at food levels
of 15 or 30 prey l−1 grew more slowly and were smaller than those provided with 60 or 120 prey l−1.
Nymphs grew more slowly when Daphnia were the only prey, than when smaller prey were available. Food level did not affect nymph growth at 10 °C or 15 °C, but
growth at 18 °C or 22 °C may have been slowed at the lowest food levels. Synergistic effects of temperature and food level
on nymph growth were apparent only from analysis of growth curves and not from stage duration data. 相似文献
190.