血脂康胶囊对冠心病患者的抗氧化及抗炎效果研究

目的:探讨血脂康胶囊对冠心病患者的抗氧化及抗炎效果和意义。方法:选取冠心病并且有一定程度的脂代谢紊乱的患者64例,按随机对照原则随机分为A组(32例)及B组(32例),A组给予血脂康胶囊600mg,日2次;B组予洛伐他汀40mg/d,丙氨酸转氨酶或天冬氨酸转氨酶升高超过正常值3倍以上的患者服用剂量减半,即洛伐他汀20mg/d,经治疗或者观察恢复正常后再加到40mg/d。共比8周,检测实验前后的血浆ALT,AST,CK,OX-LDL,CRP及SOD水平。结果:血脂康胶囊可以明显的降低血浆TG,CHOL,LDL水平,并且与对照组差异有显著性意义(P<0.05)。血脂康胶囊可以明显的降低血浆OX-LD,CRP水平,与对照组差异有非常显著性意义(P<0.01),血脂康胶囊可以提高血浆SOD水平优于对照组(P<0.01),而升高ALT,AST,CK的副作用较对照组低(P<0.05)。结论:血脂康胶囊常规剂量不仅有效调脂而安全,并且有一定的抗氧化及抗炎效果。     

血脂康胶囊对冠心病患者的抗氧化及抗炎效果研究

目的：探讨血脂康胶囊对冠心病患者的抗氧化及抗炎效果和意义。方法：选取冠心病并且有一定程度的脂代谢紊乱的患者64例,按随机对照原则随机分为A组（32例）及B组（32例）,A组给予血脂康胶囊600mg,日2次;B组予洛伐他汀40mg/d,丙氨酸转氨酶或天冬氨酸转氨酶升高超过正常值3倍以上的患者服用剂量减半,即洛伐他汀20mg/d,经治疗或者观察恢复正常后再加到40mg/d。共比8周,检测实验前后的血浆ALT,AST,CK,OX-LDL,CRP及SOD水平。结果：血脂康胶囊可以明显的降低血浆TG,CHOL,LDL水平,并且与对照组差异有显著性意义（P〈0.05）。血脂康胶囊可以明显的降低血浆OX-LD,CRP水平,与对照组差异有非常显著性意义（P〈0.01）,血脂康胶囊可以提高血浆SOD水平优于对照组（P〈0.01）,而升高ALT,AST,CK的副作用较对照组低（P〈0.05）。结论：血脂康胶囊常规剂量不仅有效调脂而安全,并且有一定的抗氧化及抗炎效果。     

Quality control of fungus-specific glucosylceramide in Cryptococcus neoformans by endoglycoceramidase-related protein 1 (EGCrP1)

A fungus-specific glucosylceramide (GlcCer), which contains a unique sphingoid base possessing two double bonds and a methyl substitution, is essential for pathogenicity in fungi. Although the biosynthetic pathway of the GlcCer has been well elucidated, little is known about GlcCer catabolism because a GlcCer-degrading enzyme (glucocerebrosidase) has yet to be identified in fungi. We found a homologue of endoglycoceramidase tentatively designated endoglycoceramidase-related protein 1 (EGCrP1) in several fungal genomic databases. The recombinant EGCrP1 hydrolyzed GlcCer but not other glycosphingolipids, whereas endoglycoceramidase hydrolyzed oligosaccharide-linked glycosphingolipids but not GlcCer. Disruption of egcrp1 in Cryptococcus neoformans, a typical pathogenic fungus causing cryptococcosis, resulted in the accumulation of fungus-specific GlcCer and immature GlcCer that possess sphingoid bases without a methyl substitution concomitant with a dysfunction of polysaccharide capsule formation. These results indicated that EGCrP1 participates in the catabolism of GlcCer and especially functions to eliminate immature GlcCer in vivo that are generated as by-products due to the broad specificity of GlcCer synthase. We conclude that EGCrP1, a glucocerebrosidase identified for the first time in fungi, controls the quality of GlcCer by eliminating immature GlcCer incorrectly generated in C. neoformans, leading to accurate processing of fungus-specific GlcCer.     

高效液相色谱法测定经舒胶囊中丹皮酚的含量

目的:建立以高效液相色谱法测定经舒胶囊中丹皮酚含量的方法。方法:采用Platisil ODS色谱柱(4.6 mm×250 mm 5μm);以甲醇-水(45:55)为流动相;柱温:40℃;流速:1.0ml.min-1;检测波长:274nm。结果:在建立的色谱条件下,丹皮酚进样量在0.08487~0.50922μg范围内与峰面积呈良好的线性关系(r=0.9999),平均回收率(n=6)为98.75%(RSD=1.74%)。结论:方法简便可靠,分离度好,可用于经舒胶囊的质量控制。     

曲美布汀联合双歧三联活菌胶囊治疗肠易激综合征

目的观察曲美布汀联合双歧三联活菌胶囊治疗肠易激综合征的临床疗效。方法将83例肠易激综合征患者随机分为A、B、C三组,A组40例给予曲美布汀联合双歧三联活菌胶囊治疗,B组24例单用曲美布汀治疗,C组19例单用双歧三联活菌胶囊治疗,疗程4周后,观察各组疗效和不良反应。结果A、B、C三组的总有效率分别为92.5%、75.0%和68.4%,三组疗效比较,差异有显著性(P<0.05)。A组疗效优于B组和C组(P<0.05),B组和C组疗效差异无显著性(P>0.05)。治疗中A组和B组出现不良反应,C组未发现不良反应,但各组差异无显著性(P>0.05)。结论曲美布汀联合双歧三联活菌胶囊治疗肠易激综合征能达到了满意的疗效,两药联合治疗效果要优于单用曲美布汀或双歧三联活菌胶囊的治疗效果。     

Immunoelectron microscopic demonstration that Renibacterium salmoninarum is encapsulated

The cell surfaces of four strains of Renibacterium salmoninarum, including the type strain Lea-7-74T, were examined by transmission electron microscopy after immuno-stabilization and staining with ruthenium red. Cells were covered with a layer of capsular material whose thickness varied between 30 to 60 nm. Our results indicate that R. salmoninarum is encapsulated.     

Capsule formation in Zymomonas mobilis grown on sucrose

A capsule formed around Zymomonas mobilis grown on sucrose, increasing in thickness with higher initial sucrose concentrations. Cryofixation and freeze-substitution electron microscopy techniques preserved this polymer matrix, unlike other techniques.L.A. Kirk and R.i. Webb are with the Department of Microbiology, The University of Queensland, Brisbane, Qld 4072, Australia; R.I. Webb is also with the Centre for Microscopy and Microanalysis and H.W. Doelle is with MIRCEN-Brisbane, both at The University of Queensland, Brisbane, Qld 4072, Australia.     

Antioxidant acitivity in vitro and in vivo of the capsule polysaccharides from Streptococcus equi subsp. zooepidemicus

Crude capsule polysaccharides (CCP) were prepared from the culture of Streptococcus equi subsp. zooepidemicus C55129 and were partially purified through an anion-exchange column chromatography to afford partially purified capsule polysaccharides (PCP). The main component of CCP and PCP was hyaluronic acid. In vitro antioxidant assay, the capsule polysaccharides showed strong inhibition of lipid peroxidation and hydroxyl radical scavenging activity and moderate 1,1-diphenyl-2-picryldydrazyl radical scavenging activity. In addition, CCP exhibited much stronger reductive power than PCP. For antioxidant testing in vivo, CCP and PCP were orally administrated over a period of 15 days in a d-galactose induced aged mice model. As results, administration of capsule polysaccharides inhibited significantly the formation of malondialdehyde in mice livers and serums and raised the activities of antioxidant enzymes and total antioxidant capacity in a dose-dependent manner. However, the antioxidant activity of CCP was lower than that of PCP. The results suggest that the capsule polysaccharides from Streptococcus equi subsp. zooepidemicus C55129 have direct and potent antioxidant activities.     

脆弱类杆菌荚膜的初步观察

本文对实验室保存的72株脆弱类杆菌(Bf)及18株其它菌株,采用培养基添加血清和短期连续传代的方法,然后用Hiss荚膜染色法染色镜检,结果显示所检Bf均有厚薄不一的荚膜,其中具有厚的荚膜的计34株,中度厚度荚膜的19株,薄的19株,而所检的卵形类杆菌,核梭杆菌等未见荚膜,作对照的产气荚膜梭菌亦有厚的荚膜。作者对Bf的荚膜与致病关系以及荚膜染色方法作了讨论。