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81.
The release of l-[3H]cysteine sulfinic acid, l-[3H]glutamatic acid and [3H]GABA from preloaded slices of various rat brain regions in response to either 30 mM K+ or veratrin was investigated. All these aminoacids were released by both depolarizing agents, which did not produce any changes in the spontaneous efflux of [3H]lysine. The K+ stimulated cysteine sulfinate release from superfused slices was found partly Ca2+-dependent in the subiculum, and mainly Ca2+-independent in the hippocampus whereas the K+-elicited glutamate release was partly Ca2+-dependent in both regions. The veratrine-induced release of both cysteine sulfinate and glutamate was blocked by verapamil in a dose-dependent way, although a small verapamil concentration independent release remained. The release pattern of both amino acids was heterogeneous, but roughly correlated among brain regions, except in the subiculum and hypothalamus.These findings demonstrate the releasability of both substances from various brain regions and suggest that those releases occur from different pools, being probably mainly of neuronal origin. They give further evidence that cysteine sulfinate as well as glutamate may serve a neurotransmitter role in the CNS. 相似文献
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Alain J. Cozzone 《Biochimie》1980,62(10):647-664
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A new method for modelling genotype x environment interaction: APLAT. The yield predicted by a crop-simulation model is developed as a Taylor series in the neighbourhood of a parameter vector of a control genotype. With this local linearisation, these genotype parameters can be estimated by a linear regression of the observed yield on the derivatives of the crop-simulation model predictions with respect to its parameters. 相似文献
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