Simple and fast purification of Escherichia coli adenylate kinase

Adenylate kinase from E. coli (strains CR341 and CR341 T28, a temperature-sensitive mutant) was purified by a two-step chromatographic procedure. The enzyme from crude extracts of both mutant and parent strain was bound to blue-Sepharose at pH 7.5, thereafter specifically eluted with 0.05 mM P1,P5-di(adenosine-5')pentaphosphate. A second chromatography on Sephadex G-100 yielded pure enzyme. E. coli adenylate kinase was strongly inhibited by P1,P5-di(adenosine-5')pentaphosphate (Ki 0.6 microM for adenylate kinase of strain CR341 and 2.1 microM in the case of mutant enzyme). After denaturation in 6 M guanidinium hydrochloride both mutant and parent adenylate kinase returned rapidly to the native, active state by dilution of guanidinium hydrochloride.     

Efficacy of Bear Deterrent Spray in Alaska

Abstract: We present a comprehensive look at a sample of bear spray incidents that occurred in Alaska, USA, from 1985 to 2006. We analyzed 83 bear spray incidents involving brown bears (Ursus arctos; 61 cases, 74%), black bears (Ursus americanus; 20 cases, 24%), and polar bears (Ursus maritimus; 2 cases, 2%). Of the 72 cases where persons sprayed bears to defend themselves, 50 (69%) involved brown bears, 20 (28%) black bears, and 2 (3%) polar bears. Red pepper spray stopped bears' undesirable behavior 92% of the time when used on brown bears, 90% for black bears, and 100% for polar bears. Of all persons carrying sprays, 98% were uninjured by bears in close-range encounters. All bear—inflicted injuries (n = 3) associated with defensive spraying involved brown bears and were relatively minor (i.e., no hospitalization required). In 7% (5 of 71) of bear spray incidents, wind was reported to have interfered with spray accuracy, although it reached the bear in all cases. In 14% (10 of 71) of bear spray incidents, users reported the spray having had negative side effects upon themselves, ranging from minor irritation (11%, 8 of 71) to near incapacitation (3%, 2 of 71). Bear spray represents an effective alternative to lethal force and should be considered as an option for personal safety for those recreating and working in bear country. (JOURNAL OF WILDLIFE MANAGEMENT 72(3):640–645; 2008)     

Inhibition by zinc of the binding of C1 to antigen-antibody complexes

Zinc sulphate in the range of 10^?4 to 2×10^?5 M prevents the binding of $\text{C}\text{1}$ to antigen antibody complexes, and the initation of the cascade of events in the classical complement pathway leading to cell lysis. Other heavy metals, Co⁺⁺, Cd⁺⁺, Cu⁺⁺, or Mn⁺⁺ were without effect in this concentration range. Zinc was ineffective when added after $\text{C}\text{1}$ was bound and failed to displace $\text{C}\text{1}$ which was already bound to antigen antibody complexes. The ability of zinc to regulate the binding of the zymogen or activated form of $\text{C}\text{1}$ to antigen-antibody complexes represents a new method of controlling the initiation of the classical complement pathway.     

水稻无脊椎动物多样性的K-L变换编目分析

K-L变换可消除样品各特征间的相关性,去除信息量较少或信息虚假的坐标轴,降低特征空间的维数,用较少量的特征描述样品,是目前最为有效的数据信息压缩抽取方法。利用K-L变换,样品之间变化较小的分类单元（目或功能群等）,不会作为重要特征保留下来。因此,数量上占优势、或害虫防治中有重要地位的分类单元并不一定是识别样品的重要特征。可以藉K-L变换明确构成样品变异的主要分类单元,从而对不同地区、不同季节的取样实践有一定的指导意义、用通常的多样性指数分析及多样性差异显著性分析无法解决这个问题。用K-L变换可大大压缩数据存储空间。对数据量很大的生物多样性分析有较大的应用价值。反映样品所携信息量的热带水稻田无脊椎动物目主要是半翅目,中腹足目,以及表腹亚目。其中,在旱季半翅目占主要地位,而在雨季中腹足目占主要地位。就无脊椎动物目的组成而言,它们是构成样品变异的主要类群,是取样中需要得到特别注意的目。反映样品信息的无脊椎动物功能群主要是外部植食类,植食多食性类,半水生水面爬行类,粗粒残物取食类,以及陆生爬行、跳跃或猎者类,是取样中要重点考虑的一些类群。     

Use of Occupancy Models to Estimate the Influence of Previous Live Captures on DNA-Based Detection Probabilities of Grizzly Bears

Abstract: Large carnivores potentially change their behavior following physical capture, becoming less responsive to the attractants that resulted in their capture, which can bias population estimates where the change in behavior is not appropriately modeled. We applied occupancy models to efficiently estimate and compare detection probabilities of previously collared grizzly bears (Ursus arctos) with bears captured at DNA hair-snag sites that were not previously collared. We found that previously captured bears had lower detection probabilities, although their detection probabilities were still >0, implying that they were still visible to be sampled via the DNA hair-snag grid, which was able to detect finer differences in capture probabilities of previously collared bears compared with Huggins closed-captures population models. To obtain relatively unbiased population estimates for DNA surveys, heterogeneity caused by previous live capture should be accounted for in the population estimator. (JOURNAL OF WILDLIFE MANAGEMENT 72(3):589–595; 2008)