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21.
A.S. Purewal 《FEMS microbiology letters》1991,82(2):229-232
The nucleotide sequence of the gene specifying the ethidium efflux system of Escherichia coli has been determined. The translated open reading frame has identified a membrane-bound polypeptide of 110 amino acids (11,960 Da) which shares 42% identity with a staphylococcal protein specifying resistance to ethidium. 相似文献
22.
V ronique Cruciani Karen-Marie Heintz Trine Hus y Eivind Hovig David J. Warren Svein-Ole Mikalsen 《Cell communication & adhesion》2004,11(5):155-171
The open reading frames of 17 connexins from Syrian hamster (using tissues) and 16 connexins from the Chinese hamster cell line V79, were fully (Cx30, Cx31, Cx37, Cx43 and Cx45) or partially sequenced. We have also detected, and partially sequenced, seven rat connexins that previously were unavailable. The expression of connexin genes was examined in some hamster organs and cultured hamster cells, and compared with wild-type mouse and the cancer-prone Min mouse. Although the expression patterns were similar for most organs and connexins in hamster and mouse, there were also some prominent differences (Cx29 and 30.3 in testis; Cx31.1 and 32 in eye; Cx46 in brain, kidney and testis; Cx47 in kidney). This suggests that some connexins have species-specific expression profiles. In contrast, there were minimal differences in expression profiles between wild type and Min mice. Species-specific expression profiles should be considered in attempts to make animal models of human connexin-associated diseases. 相似文献
23.
In order to investigate gene expression changes associated with cytotoxicity, we used cDNA arrays to monitor the expression
of over 5,000 genes in response to toxic stress in the HepG2 liver cell line. Cells were treated with cytotoxic doses of acetaminophen,
caffeine or thioacetamide for nine time points ranging from 1 to 24 h. Samples of mRNA from each time point were used to prepare
radiolabeled cDNA, which was hybridized to nylon-membrane-based cDNA arrays. High-stringency washes were applied to reduce
cross-hybridization. Analysis of spot intensities revealed that each compound led to approximately 150-250 gene expression
changes that were sustained over at least three adjacent time points. The affected genes could be classified into clusters
based on their temporal patterns of differential expression. A common set of 44 genes showed similar expression changes in
response to all three compounds. Of these changes, 90% could be confirmed by quantitative RT-PCR analysis. The results indicate
that detailed array-based time-course studies, coupled with a sensitive and highly specific confirmation assay, provide a
powerful means of identifying cytotoxicity-associated gene expression changes.
Electronic Publication 相似文献
24.
D. F. Roberts 《International Journal of Anthropology》1996,11(2-4):185-191
As a conclusion, this paper reviews briefly the content of the volume. The wealth of demographic data has not been adequately
exploited in anthropology; this is why this publication is valuable in showing attempts to apply demographic data in a variety
of anthropological problems. This symposium has explored many interesting points which we recall here. Yet it has also opened
up a whole range of further questions on the material presented as well as in this broad field. Several directions of research
could be developed, for instance, testing among human populations, over long periods, the ecological thoughts of ecosystems
evolving as a cascade of instabilities, rather than a succession of equilibrium states. Let us also recall the pervasive nature
of demographic facts in topics such as the energy cycle or the genetic structure and evolution of human populations. 相似文献
25.
Tapas K. Nandi Hridoy R. Bairagya Bishnu P. Mukhopadhyay K. Sekar Dipankar Sukul Asim K. Bera 《Journal of biosciences》2009,34(1):27-34
The role of invariant water molecules in the activity of plant cysteine protease is ubiquitous in nature. On analysing the
11 different Protein DataBank (PDB) structures of plant thiol proteases, the two invariant water molecules W1 and W2 (W220
and W222 in the template 1PPN structure) were observed to form H-bonds with the Ob atom of Asn 175. Extensive energy minimization and molecular dynamics simulation studies up to 2 ns on all the PDB and solvated
structures clearly revealed the involvement of the H-bonding association of the two water molecules in fixing the orientation
of the asparagine residue of the catalytic triad. From this study, it is suggested that H-bonding of the water molecule at
the W1 invariant site better stabilizes the Asn residue at the active site of the catalytic triad. 相似文献
26.
In searching for differentially expressed genes in human uterine leiomyomas (ULs), suppression sub-tractive hybridization was used to construct an UL up-regulated library, which turned out to represent 88genes. After two rounds of screening by reverse Northern analysis, twenty genes were proved to be up-regulated, including seventeen known genes and three genes with unknown function. All these genes werefirstly associated with UL. Three genes with notable difference were selected for Northern confirmationOur results proved the authenticity of the twenty genes. One gene named Phospholipase A2 (PLA2) showedup-regulation in 4/6 of the patients and investigation of tissue distribution indicated that it had obviousexpression in prostate, testis, liver, heart and skeletal muscle. 相似文献
27.
Sequences related to the major subunit gene fedA of F107 fimbriae in porcine Escherichia coli strains that express adhesive fimbriae 总被引:1,自引:0,他引:1
Abstract Porcine Escherichia coli strains isolated from cases fo postweaning diarrhea or edema disease were analysed for the presence of fedA , the major subunit gene of F107 fimbriae. The E. coli isolates were known to contain colonisation factor '8813', or to express F107, 2134P or other fimbriae, different from F4, F5, F6, and F41. PCR with fedA -specific primers, restriction enzyme digestion of the PCR product, and nucleotide sequence analysis demonstrated that 2134P pili, colonisation factor '8813' and fimbriae identified on Australian strains of the O141 serotype belong to one family of F107 fimbrial antigens. 相似文献
28.
The influence of contact guidance on the orientation of colonies of subcultured vascular smooth muscle cells 总被引:1,自引:0,他引:1
Robert C. Buck 《In vitro cellular & developmental biology. Plant》1982,18(9):783-788
Summary Subcultures of smooth muscle cells derived from rat thoracic aorta were grown on plane plastic substrata and on plastic substrata
having ridges molded in them by a heated, ruled template. The cells were found to have a very high degree of contact guidance
when distributed sparsely on the ridged substrata. When the cell density increased multilayered, elongated colonies formed.
On plane substrata these were irregular, curved, and disposed in all directions. On the ridged substrata, however, the colonies
were straight, evenly spaced, and positioned at right angles to the ridges.
Supported by Grant MT1011 from the Medical Research Council of Canada. 相似文献
29.
DefattedMadhuca butyraceae seeds contain 24% of crude protein and 10.4% of saponins. The solubility ofMadhuca seed proteins was determined in water and NaCl as a function of pH and minimum solubility occurred at pH 4.0. The proteins
consist of three components with S20,w values of 2.2, 9.8 and 15.4. On gel filtration the proteins gave three peaks and on diethylaminoethyl cellulose chromatography
they resolved into two components. Thein vitro digestibility ofMadhuca seed protein was found to be 69% when assayed with a pepsin-pancreatin system. 相似文献
30.
The uptake of two different preparations of99mTechnetium-methylene diphosphonate in fetal rat calvaria is compared. The localization of99mTc after administration of99mTc(Sn)-MDP and99mTc-MDP showed equal distribution in autoradiography. 相似文献