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71.
《Harmful algae》2014
The dinoflagellate Alexandrium ostenfeldii is a well-known harmful algal species that can potentially cause paralytic shellfish poisoning (PSP). Usually A. ostenfeldii occurs in low background concentrations only, but in August of 2012 an exceptionally dense bloom of more than 1 million cells L−1 occurred in the brackish Ouwerkerkse Kreek in The Netherlands. The A. ostenfeldii bloom produced both saxitoxins and spirolides, and is held responsible for the death of a dog with a high saxitoxin stomach content. The Ouwerkerkse Kreek routinely discharges its water into the adjacent Oosterschelde estuary, and an immediate reduction of the bloom was required to avoid contamination of extensive shellfish grounds. Previously, treatment of infected waters with hydrogen peroxide (H2O2) successfully suppressed cyanobacterial blooms in lakes. Therefore, we adapted this treatment to eradicate the Alexandrium bloom using a three-step approach. First, we investigated the required H2O2 dosage in laboratory experiments with A. ostenfeldii. Second, we tested the method in a small, isolated canal adjacent to the Ouwerkerkse Kreek. Finally, we brought 50 mg L−1 of H2O2 into the entire creek system with a special device, called a water harrow, for optimal dispersal of the added H2O2. Concentrations of both vegetative cells and pellicle cysts declined by 99.8% within 48 h, and PSP toxin concentrations in the water were reduced below local regulatory levels of 15 μg L−1. Zooplankton were strongly affected by the H2O2 treatment, but impacts on macroinvertebrates and fish were minimal. A key advantage of this method is that the added H2O2 decays to water and oxygen within a few days, which enables rapid recovery of the system after the treatment. This is the first successful field application of H2O2 to suppress a marine harmful algal bloom, although Alexandrium spp. reoccurred at lower concentrations in the following year. The results show that H2O2 treatment provides an effective emergency management option to mitigate toxic Alexandrium blooms, especially when immediate action is required. 相似文献
72.
《Harmful algae》2014
The detection of dense harmful algal blooms (HABs) by satellite remote sensing is usually based on analysis of chlorophyll-a as a proxy. However, this approach does not provide information about the potential harm of bloom, nor can it identify the dominant species. The developed HAB risk classification method employs a fully automatic data-driven approach to identify key characteristics of water leaving radiances and derived quantities, and to classify pixels into “harmful”, “non-harmful” and “no bloom” categories using Linear Discriminant Analysis (LDA). Discrimination accuracy is increased through the use of spectral ratios of water leaving radiances, absorption and backscattering. To reduce the false alarm rate the data that cannot be reliably classified are automatically labelled as “unknown”. This method can be trained on different HAB species or extended to new sensors and then applied to generate independent HAB risk maps; these can be fused with other sensors to fill gaps or improve spatial or temporal resolution. The HAB discrimination technique has obtained accurate results on MODIS and MERIS data, correctly identifying 89% of Phaeocystis globosa HABs in the southern North Sea and 88% of Karenia mikimotoi blooms in the Western English Channel. A linear transformation of the ocean colour discriminants is used to estimate harmful cell counts, demonstrating greater accuracy than if based on chlorophyll-a; this will facilitate its integration into a HAB early warning system operating in the southern North Sea. 相似文献
73.
《Harmful algae》2014
Harmful Cochlodinium polykrikoides blooms have frequently appeared and caused fatal harm to aquaculture in Korean coastal waters since 1995. We investigated the applicability of GOCI, the world's first Geostationary Ocean Color Imager, in monitoring the distribution and temporal movement of a harmful algal bloom (HAB) that was discovered in the East Sea near the Korean peninsula in August 2013. We identified the existence of C. polykrikoides at a maximum cell abundance of over 6000 cells/mL and a chlorophyll a concentration of over 400 mg/m3. In areas of C. polykrikoides blooms, GOCI remote sensing reflectance (Rrs) spectra demonstrated the typical radiometric features of a HAB, and from the diurnal variations using GOCI-derived chlorophyll concentration images, we were able to identify the vertical migration of the red tide species. We also found that the formation and propagation of the HAB had relations with cold water mass in the coastal region. GOCI can be effectively applied to the monitoring of short-term and long-term movements of red tides. 相似文献
74.
Nutritional insufficiency and toxicity are deleterious effects of phytoplankton on grazers. We hypothesize that toxic food is likely to have stronger evolutionary selective effects on grazers than nutritionally insufficient food. We explore this hypothesis in comparative studies of egg production and egg hatching of the copepod Acartia hudsonica challenged with both a toxic and a nutritionally insufficient alga. Experiments lasting 6 days, in which mixtures of different proportions of the suspect and a control alga were offered as food to female copepods, showed that the dinoflagellate Alexandrium fundyense, which bears paralytic shellfish toxins, was toxic to A. hudsonica. In contrast, the diatom Phaeodactylum tricornutum was nutritionally insufficient to A. hudsonica. In another set of experiments, the effects of A. fundyense and P. tricornutum, respectively, as sole foods on egg production and egg hatching success of two geographically separated populations (Maine and Connecticut) of the copepod A. hudsonica were examined in common-environment experiments, after being raised under identical conditions for two generations. The location in Maine regularly experiences toxic blooms of Alexandrium sp. whereas the location in Connecticut does not. During a 6-day period, A. fundyense reduced the egg production rates of the Connecticut copepod population, but not of the Maine population. In contrast, the diatom P. tricornutum reduced the egg production of both populations. These results of this study are consistent with the hypothesis of local adaptation to toxic food, but not to nutritionally insufficient food. 相似文献
75.
Hae Jin Jeong Tae Hoon Kim Yeong Du Yoo Eun Young Yoon Jae Seong Kim Kyeong Ah Seong Kwang Young Kim Jae Yeon Park 《Harmful algae》2011,10(6):576-588
The euglenophyte Eutreptiella gymnastica is a common red tide causative species. However, there have been no studies on the grazing impact of heterotrophic protists on this species. To investigate the grazing impact of heterotrophic protists on E. gymnastica, we measured daily the abundances of E. gymnastica and co-occurring potential heterotrophic protistan grazers in Masan Bay, Korea, in August 2004 when an E. gymnastica red tide occurred. In addition, we tested whether the common heterotrophic dinoflagellates Gyrodinium dominans, Oxyrrhis marina, Pfiesteria piscicida, Polykrikos kofoidii, Protoperidinium bipes, and Stoeckeria algicida and the naked ciliates Strobilidium sp. (30–40 μm in cell length) and Strombidinopsis sp. (70–100 μm in cell length) were able to feed on E. gymnastica. We also measured their growth and ingestion rates on E. gymnastica as a function of prey concentration. Finally, we calculated the grazing coefficients by combining field data on the abundance of the heterotrophic dinoflagellate and ciliate grazers and co-occurring E. gymnastica with laboratory data on ingestion rates obtained in this study. The maximum abundance of E. gymnastica in Masan Bay in August, 2004 was 7575 cells ml−1, while those of Gyrodinium spp., P. kofoidii, P. bipes, the naked ciliates (≤50 μm in cell length), and naked ciliates (>50 μm in cell length) were 50, 9, 58, 32, and 3 cells ml−1, respectively. The maximum growth rate of G. dominans on E. gymnastica (1.13 d−1) was higher than that of O. marina (0.81 d−1) or P. bipes (0.77 d−1). However, E. gymnastica did not support positive growth of P. kofoidii, Strobilidium sp., and Strombidinopsis sp. (−0.04 ∼ −2.8 d−1). The maximum ingestion rates of G. dominans, P. kofoidii, P. bipes, O. marina, and Strobilidium sp. on E. gymnastica (2.1–2.7 ng C predator−1 d−1) were similar, but they were much lower than that of Strombidinopsis sp. (156 ng C predator−1 d−1). The calculated grazing coefficients for P. bipes, small heterotrophic Gyrodinium spp. (25–35 μm in cell length), naked ciliates (≤50 μm in cell length), P. kofoidii, and naked ciliates (>50 μm in cell length) on E. gymnastica were up to 0.77, 0.61, 0.22, 0.07 and 0.03 d−1, respectively (i.e., up to 54%, 46%, 20%, 7%, and 3% of E. gymnastica populations were removed by the population of each of these heterotrophic protistan grazers in 1 d, respectively). The results of the present study suggest that P. bipes, small heterotrophic Gyrodinium spp., and naked ciliates (≤50 μm in cell length) sometimes have considerable potential grazing impact on the populations of E. gymnastica. 相似文献
76.
Except for the lack of a centriole, interphase cell morphology and cell division in Stichococcus is similar to that in Klebsormidium. The cell in Stichococcus is largely filled by a chloroplast and pyrenoid, at the side of which are two mitochondria and one small peroxisome. The chloroplast/pyrenoid cleaves early in prophase, probably completely, and the nucleus is inserted between the two halves. A band of 3–5 microtubules always encircles the prophase nucleus; these disappear by metaphase. The spindle is open, the daughter nuclei remain far apart at telophase and during cytokinesis, and vacuoles collect between them; no phycoplast is associated with the cleavage furrow. These results indicate a close phyletic relationship between Stichococcus and Klebsormidium, two organisms which are now considered to be more closely related to the progenitors of the higher land plants than most of the other members of the Ulotrichales. 相似文献
77.
Camacho FG Rodríguez JG Mirón AS García MC Belarbi EH Chisti Y Grima EM 《Biotechnology advances》2007,25(2):176-194
Dinoflagellates are microalgae that are associated with the production of many marine toxins. These toxins poison fish, other wildlife and humans. Dinoflagellate-associated human poisonings include paralytic shellfish poisoning, diarrhetic shellfish poisoning, neurotoxic shellfish poisoning, and ciguatera fish poisoning. Dinoflagellate toxins and bioactives are of increasing interest because of their commercial impact, influence on safety of seafood, and potential medical and other applications. This review discusses biotechnological methods of identifying toxic dinoflagellates and detecting their toxins. Potential applications of the toxins are discussed. A lack of sufficient quantities of toxins for investigational purposes remains a significant limitation. Producing quantities of dinoflagellate bioactives requires an ability to mass culture them. Considerations relating to bioreactor culture of generally fragile and slow-growing dinoflagellates are discussed. Production and processing of dinoflagellates to extract bioactives, require attention to biosafety considerations as outlined in this review. 相似文献
78.
《Harmful algae》2019
Species of the benthic dinoflagellate Gambierdiscus produce polyether neurotoxins that caused ciguatera fish/shellfish poisoning in human. The toxins enter marine food webs by foraging of herbivores on the biotic substrates like macroalgae that host the toxic dinoflagellates. Interaction of Gambierdiscus and their macroalgal substrate hosts is believed to shape the tendency of substrate preferences and habitat specialization. This was supported by studies that manifested epiphytic preferences and behaviors in Gambierdiscus species toward different macroalgal hosts. To further examine the supposition, a laboratory-based experimental study was conducted to examine the growth, epiphytic behaviors and host preferences of three Gambierdiscus species towards four macroalgal hosts over a culture period of 40 days. The dinoflagellates Gambierdiscus balechii, G. caribaeus, and a new ribotype, herein designated as Gambierdiscus type 7 were initially identified based on the thecal morphology and molecular characterization. Our results showed that Gambierdiscus species tested in this study exhibited higher growth rates in the presence of macroalgal hosts. Growth responses and attachment behaviors, however, differed among different species and strains of Gambierdiscus over different macroalgal substrate hosts. Cells of Gambierdiscus mostly attached to substrate hosts at the beginning of the experiments but detached at the later time. Localized Gambierdiscus-host interactions, as demonstrated in this study, could help to better inform efforts of sampling and monitoring of this benthic toxic dinoflagellate. 相似文献
79.
80.
González-Romero R Rivera-Casas C Fernández-Tajes J Ausió J Méndez J Eirín-López JM 《Comparative biochemistry and physiology. Toxicology & pharmacology : CBP》2012,155(2):175-181
Marine biotoxins synthesized by Harmful Algal Blooms (HABs) represent one of the most important sources of contamination in marine environments as well as a serious threat to fisheries and aquaculture-based industries in coastal areas. Among these biotoxins Okadaic Acid (OA) is of critical interest as it represents the most predominant Diarrhetic Shellfish Poisoning biotoxin in the European coasts. Furthermore, OA is a potent tumor promoter with aneugenic and clastogenic effects on the hereditary material, most notably DNA breaks and alterations in DNA repair mechanisms. Therefore, a great effort has been devoted to the biomonitoring of OA in the marine environment during the last two decades, mainly based on physicochemical and physiological parameters using mussels as sentinel organisms. However, the molecular genotoxic effects of this biotoxin make chromatin structure a good candidate for an alternative strategy for toxicity assessment with faster and more sensitive evaluation. To date, the development of chromatin-based studies to this purpose has been hampered by the complete lack of information on chromatin of invertebrate marine organisms, especially in bivalve molluscs. Our preliminary results have revealed the presence of histone variants involved in DNA repair and chromatin specialization in mussels and clams. In this work we use this information to put forward a proposal focused on the development of chromatin-based tests for OA genotoxicity in the marine environment. The implementation of such tests in natural populations has the potential to provide an important leap in the biomonitoring of this biotoxin. The outcome of such monitoring may have critical implications for the evaluation of DNA damage in these marine organisms. They will provide as well important tools for the optimization of their harvesting and for the elaboration of additional tests designed to evaluate the safety of their consumption and potential implications for consumer's health. 相似文献