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81.
《Molecular & cellular proteomics : MCP》2019,18(10):2108-2120
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- •Bayesian Beta-Binomial model integrates ion statistics with peptide ratio agreement.
- •Model appropriately interprets information from low signal peptides.
- •Confidence can be assigned even without replicates.
- •Model adds sensitivity to detection of small changes.
82.
83.
《Molecular & cellular proteomics : MCP》2019,18(6):1227-1241
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- •Quantitative microproteomics to study the CNS and PNS of the Twitcher mouse.
- •10plex TMT experiments on corpus callosum, motor cortex and sciatic nerves extracts.
- •More than 400 proteins groups deregulated between Twitcher and wildtype mice.
- •New insights into the molecular mechanisms of Krabbe disease.
84.
85.
《Molecular & cellular proteomics : MCP》2019,18(1):151-161
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- •New MALDI MS imaging sample preparation workflow reveals tissue protease activity.
- •Differential time- and inhibitor concentration-dependence confirm active proteases.
- •Mouse gastric tumor displays high protease activity compared to surrounding tissue.
- •Proteomic data and biochemical protease activity assay support MALDI MSI results.
86.
《Molecular & cellular proteomics : MCP》2019,18(8):1491-1510
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- •HLA-B*51 and ERAP1, but not ERAP2, are risk factors for Behçet's disease.
- •The HLA-B*51 peptidome and the effects of ERAP1 and ERAP2 on it are analyzed.
- •ERAP1 and ERAP2 alter multiple features of the HLA-B*51 peptidome in distinct ways.
- •Both enzymes act independently with complementary and partially redundant functions.
87.
《Molecular & cellular proteomics : MCP》2019,18(1):51-64
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- •microRNA-222 attenuates TGEV-induced mitochondrial dysfunction.
- •microRNA-222 downregulates THBS1 and CD47.
- •THBS1 is the target of microRNA-222 during TGEV infection.
- •THBS1 and CD47 increase mitochondrial Ca2+ level and reduced mitochondrial membrane potential (MMP).
88.
Intact Transition Epitope Mapping – Targeted High-Energy Rupture of Extracted Epitopes (ITEM-THREE),
《Molecular & cellular proteomics : MCP》2019,18(8):1543-1555
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- •Multiplex epitope mapping/antigenic determinant identification in the gas phase.
- •Intact transition and controlled dissociation of immune complexes by MS.
- •Simultaneous identification and amino acid sequence determination of epitopes.
- •Simplified in-solution sample handling because of ion manipulation and filtering by MS.
89.
《Molecular & cellular proteomics : MCP》2019,18(3):534-545
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- •Glycosylation of endogenous FcγRIII from neutrophils and matched plasma from more than 40 donors characterized at two sites involved in IgG binding.
- •Glycosylation of soluble FcγRIII glycosylation at N45 can be used to assign FcγRIIIb alleles.
- •FcγRIIIb allele specific differences in glycosylation at N162 may influence differential activity observed for primary cells.
90.
《Molecular & cellular proteomics : MCP》2019,18(3):477-489
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- •Developed a data processing pipeline to format phosphopeptide identifications.
- •Identified the preferred substrate motif for FLT3 and mutant kinases.
- •Designed and validated a panel of pan-FTL3 artificial substrates.
- •Monitored FLT3 and mutant kinase activity through FAStide phosphorylation.