绞股蓝悬浮细胞的原生质体再生植株

绞股蓝(Gynostemma pentaphyllum (Thumb)Mak．)是葫芦科多年生草本药用植物,现已得到广泛的开发利用,本文首次报道了绞股蓝悬浮细胞的原生质体再生植株。     

广西绞股蓝属药用植物一新种

<正> 扁果绞股蓝 新种 美七脉(壮语) Gynostemma compressum X. X. Chen et D. R. Liang, sp. nov. Species affinis G. guangxiensi X. X. Chen et D. R. Liang, quae foliis pedatis 5-foliolatis, foliolis obovatis vel ovaetooblongis, apice obtusis, fruetibus triquetro-globosis, seminibus oblongis, margine osulcatis differt. Herbae scandentes. Caulis gracilis, sulcatus, glaber. Cirrhi filiformes,     

绞股蓝繁殖试验研究

报道绞股监种子繁殖和扦插繁殖的试验结果,为绞股蓝人工培提供繁殖技木。     

绞股蓝饮料的开发研究

以绞股蓝为主要原料,配以甘草、蜂蜜等,选择出适宜的配方和工艺条件,研制出色、香、味俱佳,清凉止渴,具有一定保健功能的天然饮品,有助于绞股蓝这一野生植物资源的开发利用.     

Cardiovascular effects of the aqueous extract of Gynostemma pentaphyllum Makino

In the present study, the cardiovascular activity of the aqueous extract of Gynostemma pentaphyllum Makino leaves was investigated in the anaestetized guinea-pigs and has been compared with two of its isolated gypenosides (III, VIII) and with verapamil, a well-known Ca-antagonistic drug. The results obtained showed that the intravenous administration of the decoction of G. pentaphyllum (2.5, 5 and 10mg/kg) produced a protective effect against pitressin-induced coronaryspasm, arrhythmias and pressor response. Extract also increased the dose of ouabain required to cause ventricular tachyarrhythmias and lethality. Further extract reversed ouabain-induced persistent ventricular tachycardia and restored sinus rhythm in a dose-dependent manner. The results obtained have also shown that gypenosides III and VIII caused similar protective effects in both experimental models used; however, the duration of the action is lower than that of the extract containing corresponding quantities of gypenosides III and VIII.     

绞股蓝龙须茶的人参皂甙组织定位及皂甙含量的季节变化

应用组织化学定位技术及植物化学方法,研究了人参皂甙在商品绞股蓝龙须茶中的组织分布状态及绞股蓝总皂甙在不同季节中的含量变化。结果表明,绞股蓝内的人参皂甙遇特定的显色剂出现深浅不同的红色,色度与人参皂甙含量呈正相关。绞股蓝叶内的人参皂甙主要分布在叶肉组织,茎内的人参皂甙主要分布于维管束的韧皮部薄壁细胞中,同化组织、厚角组织和表皮也有少量分布．木质部和髓薄壁组织中无皂甙分布。在4月至11月的龙须茶采收期中,总皂甙含量呈逐渐上升的势态,4月最低．9月最高,含量变化与月总降雨量呈显著的正相关。研究认为在8月后湿润凉爽气候条件下采摘绞股蓝芽尖加工而成的龙须茶品质最好。     

Morphological and structural characterization of a polysaccharide from Gynostemma pentaphyllum Makino and its anti-exercise fatigue activity

The crude polysaccharide was obtained from Gynostemma pentaphyllum Makino by water extraction followed by ethanol precipitation. The polysaccharide was successively purified by chromatography on DEAE-52 and SephadexG-150 column, and three polysaccharide fractions were obtained and termed GPP1-a, GPP2-b, and GPP3-a, respectively. The administration with GPP1-a markedly prolonged exhaustive exercise time of the mice. Structural features of GPP1-a were investigated by a combination of instrumental and chemical analyses, including atomic force microscope (AFM), scanning electron microscope (SEM), partial acid hydrolysis, periodate oxidation, Smith degradation, methylation analysis, gas chromatography–mass spectrometry (GC–MS) analysis and NMR spectroscopy. The results indicate that GPP1-a has a backbone of (1 → 4)-linked α-d-Glucose residues, which occasionally branches at O-6. The branches are mainly composed of (1 → 6)-linked α-d-Glucose, (1 → 3)-linked β-d-Galactose and (1 → 6)-linked α-d-Galactose residues, and terminated with β-d-Galactose residues and β-l-Arabinose residues.     

云南西双版纳野生与栽培绞股蓝根内丛枝菌根真菌的分子多样性

采用巢式-PCR、DNA克隆测序技术,对西双版纳地区野生与栽培绞股蓝根内丛枝菌根真菌(AMF)进行群落结构多样性研究.试验共获得551个含有丛枝菌根真菌18S rDNA 片段的克隆子,经限制性片段长度多态性分析,得到100个RFLP类型,将其划分为20个序列类型,分属于7个科.将20个序列类型的相关序列与GeneBank数据库进行比对,有5个可以鉴定到种,分别为Glomus viscosum、Claroideoglomus etunicatum、Racocetra tropicana、Acaulospora spinosa、Acaulospora mellea.与 MaarjAM数据库中序列进行比对,12个可鉴定为虚拟分类分子种,其中7个未在孢子形态学鉴定方法中获得.西双版纳地区野生与栽培绞股蓝根内丛枝菌根真菌群落组成差异极显著.野生绞股蓝根内Glo₂、Amb₁、Para₁为优势类群,而栽培绞股蓝根内Glo₃、Glo₈、Glo₁₀、Div₁为优势类群.Claroideoglomeraceae和Ambisporaceae仅在野生样本中出现,而Diversisporaceae仅在栽培样品中出现.     

土壤肥力对绞股蓝种群行为的影响

利用半控制实验生态学方法探讨土壤肥力对绞股蓝种群行为的影响。结果表明：土壤肥力的提高可显著提高繁殖器官生物量分配比,显著降低非繁殖器官生物量分配比,叶片、支持结构生物量分配随土壤肥力的提高略有降低;提高土壤肥力对绞股蓝种群形态特征和平均相对生长速率的影响不明显;多数繁殖特征对土壤肥力的提高有显著响应。总之,绞股蓝种群的不同行为对土壤肥力变化的敏感性各异     

绞股蓝的抗肿瘤作用

本文综述了绞股蓝的抗肿瘤作用及具有抗肿瘤活性的绞股蓝皂甙的结构特点、作用机理、构效关系和这种抗肿瘤新药的研究进展。