全文获取类型
收费全文 | 320篇 |
免费 | 17篇 |
国内免费 | 76篇 |
出版年
2024年 | 1篇 |
2023年 | 7篇 |
2022年 | 10篇 |
2021年 | 11篇 |
2020年 | 10篇 |
2019年 | 6篇 |
2018年 | 6篇 |
2017年 | 8篇 |
2016年 | 7篇 |
2015年 | 8篇 |
2014年 | 15篇 |
2013年 | 18篇 |
2012年 | 5篇 |
2011年 | 13篇 |
2010年 | 9篇 |
2009年 | 27篇 |
2008年 | 16篇 |
2007年 | 15篇 |
2006年 | 20篇 |
2005年 | 23篇 |
2004年 | 18篇 |
2003年 | 22篇 |
2002年 | 20篇 |
2001年 | 10篇 |
2000年 | 7篇 |
1999年 | 10篇 |
1998年 | 12篇 |
1997年 | 3篇 |
1996年 | 5篇 |
1995年 | 4篇 |
1994年 | 6篇 |
1993年 | 8篇 |
1992年 | 4篇 |
1991年 | 5篇 |
1990年 | 2篇 |
1989年 | 6篇 |
1988年 | 6篇 |
1986年 | 3篇 |
1985年 | 7篇 |
1984年 | 4篇 |
1983年 | 1篇 |
1982年 | 3篇 |
1981年 | 1篇 |
1980年 | 1篇 |
1979年 | 3篇 |
1977年 | 1篇 |
1976年 | 2篇 |
1975年 | 1篇 |
1974年 | 3篇 |
排序方式: 共有413条查询结果,搜索用时 46 毫秒
141.
Physical obstructions that reduce space for root growth can profoundly affect plant performance. The aim of this study was to investigate the ability of roots to avoid obstructions and forage for usable space, and to reveal the mechanism involved. Eight grass species from four genera were examined. Each genus included species characteristic of habitats with high and low nutrient availability. The ability to limit root mass and to adjust morphology within substrate containing obstructions in the form of gravel was investigated. A treatment with activated carbon, which adsorbs organic compounds, was used to examine the possible involvement of root exudates in responses to obstructions. Only species characteristic of nutrient-poor habitats restricted placement of root mass in substrate containing obstructions, and this response disappeared in the presence of activated carbon. Root morphological responses to obstructions differed from those shown in response to nutrient-poor conditions or compacted soil. These results suggest that the ability to avoid obstructions is dependent on the sensitivity of roots to their own exudates accumulating in the vicinity of obstructions. This is similar to other behavioural responses in which cues or signals are used to adjust growth before stressful conditions are encountered. 相似文献
142.
Phytase activity in tobacco (Nicotiana tabacum) root exudates is exhibited by a purple acid phosphatase 总被引:2,自引:0,他引:2
Phytases are enzymes that catalyze liberation of inorganic phosphates from phytate, the major organic phosphorus in soil. Tobacco (Nicotiana tabacum) responds to phosphorus starvation with an increase in extracellular phytase activity. By a three-step purification scheme, a phosphatase with phytase activity was purified 486-fold from tobacco root exudates to a specific activity of 6,028 nkat mg(-1) and an overall yield of 3%. SDS-PAGE revealed a single polypeptide of 64 kDa, thus indicating apparent homogeneity of the final enzyme preparation. Gel filtration chromatography suggested that the enzyme was a ca. 56 kDa monomeric protein. De novo sequencing by tandem mass spectrometry resulted in a tryptic peptide sequence that shares high homology with several plant purple acid phosphatases. The identity of the enzyme was further confirmed by molybdate-inhibition assay and cDNA cloning. The purified enzyme exhibited pH and temperature optima at 5.0-5.5 and 45 degrees C, respectively, and were found to have high affinities for both p-nitrophenyl phosphate (pNPP; K(m)=13.9 microM) and phytate (K(m)=14.7 microM), but a higher kcat for pNPP (2,056 s(-1)) than phytate (908 s(-1)). Although a broad specificity of the enzyme was observed for a range of physiological substrates in soil, maximum activity was achieved using mononucleotides as substrates. We conclude that the phytase activity in tobacco root exudates is exhibited by a purple acid phosphatase and its catalytic properties are pertinent to its role in mobilizing organic P in soil. 相似文献
143.
An area of coastal dune grassland, dominated by the gramineous species Calamagrostis epigeios and Carex arenaria, was exposed to enhanced levels of UV-B radiation during a five year period. These species showed reduced AM-fungal infection percentages in their roots. In C. epigeios AM infection was reduced by 18%, C. arenaria showed a reduction by 20%. The major effect of enhanced UV-B on AM associations was a reduction of the number of arbuscules. This indicates a reduction in the exchange of nutrients between the symbionts. Since the effect of UV-B on AM associations may result from altered flavonoid levels in the root exudates of the host plants, flavonoid levels in the roots were investigated. No detectable flavonoid concentrations were found in the roots of C. epigeios and C. arenaria. Less effective AM associations can have pronounced negative effects on biodiversity and nutrient dynamics of the dune grassland ecosystem. The possible mechanisms causing these indirect effects of elevated UV-B on below ground AM associations are discussed. We conclude that UV-B induced changes in plant hormone levels are more likely to be the mechanism reducing AMF infection than UV-B induced alterations in flavonoid concentrations in the root exudates of the host plant. 相似文献
144.
Atmospheric CO2 enrichment and drought stress modify root exudation of barley 总被引:1,自引:0,他引:1 下载免费PDF全文
Olga C. Calvo Jürgen Franzaring Iris Schmid Matthias Müller Nolwenn Brohon Andreas Fangmeier 《Global Change Biology》2017,23(3):1292-1304
Rising CO2 concentrations associated with drought stress is likely to influence not only aboveground growth, but also belowground plant processes. Little is known about root exudation being influenced by elements of climate change. Therefore, this study wanted to clarify whether barley root exudation responds to drought and CO2 enrichment and whether this reaction differs between an old and a recently released malting barley cultivar. Barley plants were grown in pots filled with sand in controlled climate chambers at ambient (380 ppm) or elevated (550 ppm) atmospheric [CO2] and a normal or reduced water supply. Root exudation patterns were examined at the stem elongation growth stage and when the inflorescences emerged. At both dates, root exudates were analyzed for different compounds such as total free amino acids, proline, potassium, and some phytohormones. Elevated [CO2] decreased the concentrations in root exudates of some compounds such as total free amino acids, proline, and abscisic acid. Moreover, reduced water supply increased proline, potassium, electric conductivity, and hormone concentrations. In general, the modern cultivar showed higher concentrations of proline and abscisic acid than the old one, but the cultivars responded differentially under elevated CO2. Plant developmental stage had also an impact on the root exudation patterns of barley. Generally, we observed significant effects of CO2 enrichment, watering levels, and, to a lesser extent, cultivar on root exudation. However, we did not find any mitigation of the adverse effects of drought by elevated CO2. Understanding the multitude of relationships within the rhizosphere is an important aspect that has to be taken into consideration in the context of crop performance and carbon balance under conditions of climate change. 相似文献
145.
Potato Root Exudation and Release of Spongospora subterranea Resting Spore Germination Stimulants are Affected by Plant and Environmental Conditions 下载免费PDF全文
Mark A. Balendres David S. Nichols Robert S. Tegg Calum R. Wilson 《Journal of Phytopathology》2017,165(1):64-72
Variation in plant and environmental conditions were studied to determine the effect thereof on the exudation of low‐molecular‐weight organic compounds by potato roots. The results of the phytochemical analyses showed that among the conditions investigated, root vigour, potato cultivar, nutrients in incubation solution and temperature influenced the number and the type of primary metabolites released. Moreover, these conditions influenced our detection of compounds known to stimulate germination of resting spores of the pathogen Spongospora subterranea, causal agent of powdery scab and root diseases of potato. We conclude that changes in plant and environmental conditions can affect the release of specific compounds that stimulate germination of S. subterranea resting spores. The impact of the factors affecting potato root exudation on subsequent disease development is discussed. 相似文献
146.
土壤有机质和外源有机物对甲烷产生的影响 总被引:27,自引:2,他引:25
对土壤有机质含量及组分、外源有机物和根系分泌对甲烷产生的影响作了综述。土壤产甲烷量和甲烷排放量随有机质含量增加而提高,与土壤中易矿化有机碳或沸水浸提有机碳含量呈显著相关。外源有机碳加入促进了土壤排放甲,刺激效果与外源有机碳的用量和组成有关。还原力强的有机物如纤维素和半纤维素较还原力弱的有机物如类脂和多糖能够产生更多的甲烷。甲醇、甲基化氨基酸等无其它微生物竞争利用的有机物能被产甲烷菌更多地转化成甲烷。植物根系分泌物也促进甲烷的产生,促进作用大小与植物种类及分泌物的数量和质量有关。外源有机物通过3种方式促进土壤甲烷产生;提高土壤的甲烷底物供应量,降低土壤氧化还原电位,刺激土壤原有有机碳的转化。 相似文献
147.
The influence of temperature and olive root exudates on Heterodera mediterranea egg hatch and the effects of H. mediterranea on the growth of two olive cultivars (Arbequina and Picual) were investigated. Egg hatch occurred over a temperature range of 10 to 30°C and was optimal at 20 to 25°C. There were no differences in egg hatch between sterile deionized distilled water or root exudate dilutions (undiluted, diluted 1:1, and 1:2) of Arbequina and Picual at 20°C. Heterodera mediterranea reproduced on both olive cultivars in growth chambers at 25°C. Soil and root final nematode populations, as well as total number of cysts per plant and reproduction rate, were significantly higher in Arbequina than in Picual. Shoot dry and root fresh weights as well as increases of shoot height, trunk diameter, and numbers of nodes were significantly suppressed by infection with 10,000 eggs + second-stage juveniles/pot in Arbequina but not in Picual. 相似文献
148.
Of the 56 species and 43 genera of Asteraceae tested, 9 were highly resistant or immune to Meloidogyne incognita and did not form root galls. Twenty-six species and six cultivars had 25% or fewer roots galled and were considered moderately resistant to M. incognita. Pre-planting Cosmos bipinnatus (F190), Gaillardia pulchella, Tagetes erecta, Tithonia diversifolia, or Zinnia elegans (F645) reduced root galling and M. incognita J2 in and around Ipomoea reptans. Amendment of soils with roots, stems, or leaves of G. pulchella was effective in controlling M. incognita on I. reptans. Tissue extracts of G. pulchella were lethal to various plant-parasitic nematodes but were innocuous to free-living nematodes. Root exudates of G. pulchella were lethal to J2 of M. incognita and were inhibitory to the hatch of eggs at the concentration of 250 ppm or higher. Gaillardia pulchella could be used to manage M. incognita as a rotation crop, a co-planted crop, or a soil amendment for control of root-knot nematode. 相似文献
149.
Summary Procedures for callus induction and subsequent organogenesis in the aquatic plant, water chestnut (Trapa japonica Flerov), were established. Phenolics exuded from explants at the callus-induction stage adversely affect callus growth. For
cotyledonary node-derived callus cultured in Murashige and Skoog (MS) medium (full, half or quarter strength) containing 2,4-dichlorophenoxyacetic
acid (2,4-D) alone or in combination with benzyladenine (BA), the accumulation of phenolics was reduced and callus induction
increased by the addition of 10.8 μM phloroglucinol (PG) to the medium. Ascorbic acid was also effective in reducing phenolic accumulation, but less effective
for callus induction than PG. Half-strength MS medium supplemented with 2.7 μM 2,4-D, 108.0 μM casein hydrolyzate, and 10.8 μM PG supported maximum callus induction. Plant organogenesis was increased by addition of vitamins (0.27 μM biotin and 2.7 μM folic acid) to half-strength MS medium supplemented with 0.27 μM BA. Many shoots developed from the regenerated nodal shoot explants in liquid half-strength MS salts medium supplemented
with 1.08 μM BA and 0.27 μM naphthaleneacetic acid. Individual shoots were excised and cultured in liquid half-strength MS medium supplemented with 5.4
μM IBA and rooted plantlets (108) were transferred and acclimatized in plastic pots. After 3 wk, the plantlets were transplanted
in a water chestnut field and the survival rate was 100%. 相似文献
150.
Shoot P status regulates cluster-root growth and citrate exudation in Lupinus albus grown with a divided root system 总被引:12,自引:0,他引:12
The present study was carried out to investigate whether the P concentration in the roots or the shoots controls the growth and citrate exudation of cluster roots in white lupin (Lupinus albus L). Foliar P application indicated that low P concentration in the shoots enhanced cluster‐root growth and citrate‐exudation rate more so than low P concentration in the roots. In the split‐root study, the P concentration in the shoots increased with increased P supply (1, 25 or 75 mmol m?3 P), to the ‘privileged’ root halves. Roots ‘deprived’ of P invariably had the same low P concentrations, whereas those in the ‘privileged’ roots increased with increasing P supply (1, 25 or 75 mmol m?3 P). Nevertheless, the proportion of the total root mass allocated to cluster roots, and the citrate‐exudation rates from the root halves were always similar on both root halves, irrespective of P supply, and decreased with increasing shoot P concentrations. Peak citrate exudation rates from developing cluster roots were significantly faster from cluster roots on the ‘deprived’ root halves when the ‘privileged’ half was exposed to 1 mmol m?3 P as compared with 25 or 75 mmol m?3 P. The possibility that changes in the concentrations of P fractions in the root halves influenced cluster‐root growth and citrate exudation was discounted, because there were no significant differences in insoluble organic P, ester‐P and inorganic P among all ‘deprived’ root halves. The results indicate that cluster‐root proportions and citrate exudation rates were regulated systemically by the P status of the shoot, and that P concentrations in the roots had little influence on growth and citrate exudation of cluster roots in L. albus. 相似文献