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131.
Mechanisms of microbially enhanced Fe acquisition in red clover (Trifolium pratense L.) 总被引:2,自引:0,他引:2
Soil microorganisms may play an important role in plant Fe uptake from soils with low Fe bioavailability, but there is little direct experimental evidence to date. We grew red clover, an Fe-efficient leguminous plant, in a calcareous soil to investigate the role of soil microbial activity in plant Fe uptake. Compared with plants grown in non-sterlie (NS) grown plants, growth and Fe content of the sterile(s) grown plants was significantly inhibited, but was improved by foliar application of Fe EDTA, indicating that soil microbial activity should play an important role in plant Fe acquisition. When soil solution was incubated with phenolic root exudates from Fe-deficient red clover, a few microbial species thrived while growth of the rest was inhibited, suggesting that the Fe-deficient (-Fe) root exudates selectively influenced the rhizosphere's microbial community. Eighty six per cent of the phenolic-tolerant microbes could produce siderophore [the Fe(III) chelator] under -Fe conditions, and 71% could secrete auxin-like compounds. Interestingly, the synthetic and microbial auxins (MAs) significantly enhanced the Ferric reduction system, suggesting that MAs, in addition to siderophores, are important to plant Fe uptake. Finally, plant growth and Fe uptake in sterilized soil were significantly increased by rhizobia inoculation. Root Fe-EDTA reductase activity in the -Fe plant was significantly enhanced by rhizobia infection, and the rhizobia could produce auxin but not siderophore under Fe-limiting conditions, suggesting that the contribution of nodulating rhizobia to plant Fe uptake can be at least partially attributed to stimulation of turbo reductase activity through nodule formation and auxin production in the rhizosphere. Based on these observations, we propose as a model that root exudates from -Fe plants selectively influence the rhizosphere microbial community, and the microbes in turn favour plant Fe acquisition by producing siderophores and auxins. 相似文献
132.
Contribution of plant photosynthates to dissolved organic carbon in a flooded rice soil 总被引:1,自引:0,他引:1
Dissolved organic C (DOC) plays important roles in nutrient cycling and methane production in flooded rice ecosystem. The microcosm experiment was carried out to measure directly the contribution of photosynthates to DOC by using a 13C pulse-chase labeling technique. DOC was operationally divided into water-extractable organic C (WEOC) and salt-extractable organic C (SEOC) by successive extraction firstly with deionized water and then with 0.25 M K2SO4. Total WEOC increased with plant growth, whereas SEOC concentration did not change significantly over the growing season. About 0.037–0.36% (mean 0.16%) of the assimilated 13C was incorporated into WEOC immediately after 13CO2 assimilation (Day 0), but only 0–0.025% (mean 0.01%) was incorporated into SEOC. At the end of the growing season, the 13C amounts of WEOC substantially decreased, while those of SEOC slightly increased. The estimated net plant C contribution was 21 mg C plant–1 to WEOC and 6 mg C plant–1 to SEOC, corresponding to 33.8% of total WEOC and 20.2% of total SEOC at the end of the growing season, respectively. The results suggest that the incorporation and decomposition of the photosynthesized C occurred rapidly in rice soil which significantly affected the WEOC dynamics, but SEOC appeared to be in equilibrium with the native soil organic matter, receiving less effect from the plant growth. 相似文献
133.
134.
Miguel A. Piñeros Geraldo M. A. Cançado Lyza G. Maron Sangbom M. Lyi Marcelo Menossi Leon V. Kochian 《The Plant journal : for cell and molecular biology》2008,53(2):352-367
The phytotoxic effects of aluminum (Al) on root systems of crop plants constitute a major agricultural problem in many areas of the world. Root exudation of Al-chelating molecules such as low-molecular-weight organic acids has been shown to be an important mechanism of plant Al tolerance/resistance. Differences observed in the physiology and electrophysiology of root function for two maize genotypes with contrasting Al tolerance revealed an association between rates of Al-activated root organic acid release and Al tolerance. Using these genotypes, we cloned ZmALMT1 , a maize gene homologous to the wheat ALMT1 and Arabidopsis AtALMT1 genes that have recently been described as encoding functional, Al-activated transporters that play a role in tolerance by mediating Al-activated organic acid exudation in roots. The ZmALMT1 cDNA encodes a 451 amino acid protein containing six transmembrane helices. Transient expression of a ZmALMT1 ::GFP chimera confirmed that the protein is targeted to the plant cell plasma membrane. We addressed whether ZmALMT1 might underlie the Al-resistance response (i.e. Al-activated citrate exudation) observed in the roots of the Al-tolerant genotype. The physiological, gene expression and functional data from this study confirm that ZmALMT1 is a plasma membrane transporter that is capable of mediating elective anion efflux and influx. However, gene expression data as well as biophysical transport characteristics obtained from Xenopus oocytes expressing ZmALMT1 indicate that this transporter is implicated in the selective transport of anions involved in mineral nutrition and ion homeostasis processes, rather than mediating a specific Al-activated citrate exudation response at the rhizosphere of maize roots. 相似文献
135.
The present study was undertaken to evaluate the gum exudates of Terminalia catappa Linn. (TC gum) as a release retarding excipient in oral controlled drug delivery system. The rheological properties of TC
gum were studied and different formulation techniques were used to evaluate the comparative drug release characteristics.
The viscosity was found to be dependent on concentration and pH. Temperature up to 60°C did not show significant effect on
viscosity. The rheological kinetics evaluated by power law, revealed the shear thinning behavior of the TC gum dispersion
in water. Matrix tablets of TC gum were prepared with the model drug dextromethorphan hydrobromide (DH) by direct compression,
wet granulation and solid dispersion techniques. The dissolution profiles of the matrix tablets were compared with the pure
drug containing capsules using the USP Basket apparatus with 500 ml phosphate buffer of pH 6.8 as a dissolution medium. The
drug release from the compressed tablets containing TC gum was comparatively sustained than pure drug containing capsules.
Even though all the formulation techniques showed reduction of dissolution rate, aqueous wet granulation showed the maximum
sustained release of more than 8 h. The release kinetics estimated by the power law revealed that the drug release mechanism
involved in the dextromethorphan matrix is anomalous transport as indicated by the release exponent n values. Thus the study confirmed that the TC gum might be used in the controlled drug delivery system as a release-retarding
polymer. 相似文献
136.
137.
Physical obstructions that reduce space for root growth can profoundly affect plant performance. The aim of this study was to investigate the ability of roots to avoid obstructions and forage for usable space, and to reveal the mechanism involved. Eight grass species from four genera were examined. Each genus included species characteristic of habitats with high and low nutrient availability. The ability to limit root mass and to adjust morphology within substrate containing obstructions in the form of gravel was investigated. A treatment with activated carbon, which adsorbs organic compounds, was used to examine the possible involvement of root exudates in responses to obstructions. Only species characteristic of nutrient-poor habitats restricted placement of root mass in substrate containing obstructions, and this response disappeared in the presence of activated carbon. Root morphological responses to obstructions differed from those shown in response to nutrient-poor conditions or compacted soil. These results suggest that the ability to avoid obstructions is dependent on the sensitivity of roots to their own exudates accumulating in the vicinity of obstructions. This is similar to other behavioural responses in which cues or signals are used to adjust growth before stressful conditions are encountered. 相似文献
138.
Phytase activity in tobacco (Nicotiana tabacum) root exudates is exhibited by a purple acid phosphatase 总被引:2,自引:0,他引:2
Phytases are enzymes that catalyze liberation of inorganic phosphates from phytate, the major organic phosphorus in soil. Tobacco (Nicotiana tabacum) responds to phosphorus starvation with an increase in extracellular phytase activity. By a three-step purification scheme, a phosphatase with phytase activity was purified 486-fold from tobacco root exudates to a specific activity of 6,028 nkat mg(-1) and an overall yield of 3%. SDS-PAGE revealed a single polypeptide of 64 kDa, thus indicating apparent homogeneity of the final enzyme preparation. Gel filtration chromatography suggested that the enzyme was a ca. 56 kDa monomeric protein. De novo sequencing by tandem mass spectrometry resulted in a tryptic peptide sequence that shares high homology with several plant purple acid phosphatases. The identity of the enzyme was further confirmed by molybdate-inhibition assay and cDNA cloning. The purified enzyme exhibited pH and temperature optima at 5.0-5.5 and 45 degrees C, respectively, and were found to have high affinities for both p-nitrophenyl phosphate (pNPP; K(m)=13.9 microM) and phytate (K(m)=14.7 microM), but a higher kcat for pNPP (2,056 s(-1)) than phytate (908 s(-1)). Although a broad specificity of the enzyme was observed for a range of physiological substrates in soil, maximum activity was achieved using mononucleotides as substrates. We conclude that the phytase activity in tobacco root exudates is exhibited by a purple acid phosphatase and its catalytic properties are pertinent to its role in mobilizing organic P in soil. 相似文献
139.
An area of coastal dune grassland, dominated by the gramineous species Calamagrostis epigeios and Carex arenaria, was exposed to enhanced levels of UV-B radiation during a five year period. These species showed reduced AM-fungal infection percentages in their roots. In C. epigeios AM infection was reduced by 18%, C. arenaria showed a reduction by 20%. The major effect of enhanced UV-B on AM associations was a reduction of the number of arbuscules. This indicates a reduction in the exchange of nutrients between the symbionts. Since the effect of UV-B on AM associations may result from altered flavonoid levels in the root exudates of the host plants, flavonoid levels in the roots were investigated. No detectable flavonoid concentrations were found in the roots of C. epigeios and C. arenaria. Less effective AM associations can have pronounced negative effects on biodiversity and nutrient dynamics of the dune grassland ecosystem. The possible mechanisms causing these indirect effects of elevated UV-B on below ground AM associations are discussed. We conclude that UV-B induced changes in plant hormone levels are more likely to be the mechanism reducing AMF infection than UV-B induced alterations in flavonoid concentrations in the root exudates of the host plant. 相似文献
140.
Atmospheric CO2 enrichment and drought stress modify root exudation of barley 总被引:1,自引:0,他引:1
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Olga C. Calvo Jürgen Franzaring Iris Schmid Matthias Müller Nolwenn Brohon Andreas Fangmeier 《Global Change Biology》2017,23(3):1292-1304
Rising CO2 concentrations associated with drought stress is likely to influence not only aboveground growth, but also belowground plant processes. Little is known about root exudation being influenced by elements of climate change. Therefore, this study wanted to clarify whether barley root exudation responds to drought and CO2 enrichment and whether this reaction differs between an old and a recently released malting barley cultivar. Barley plants were grown in pots filled with sand in controlled climate chambers at ambient (380 ppm) or elevated (550 ppm) atmospheric [CO2] and a normal or reduced water supply. Root exudation patterns were examined at the stem elongation growth stage and when the inflorescences emerged. At both dates, root exudates were analyzed for different compounds such as total free amino acids, proline, potassium, and some phytohormones. Elevated [CO2] decreased the concentrations in root exudates of some compounds such as total free amino acids, proline, and abscisic acid. Moreover, reduced water supply increased proline, potassium, electric conductivity, and hormone concentrations. In general, the modern cultivar showed higher concentrations of proline and abscisic acid than the old one, but the cultivars responded differentially under elevated CO2. Plant developmental stage had also an impact on the root exudation patterns of barley. Generally, we observed significant effects of CO2 enrichment, watering levels, and, to a lesser extent, cultivar on root exudation. However, we did not find any mitigation of the adverse effects of drought by elevated CO2. Understanding the multitude of relationships within the rhizosphere is an important aspect that has to be taken into consideration in the context of crop performance and carbon balance under conditions of climate change. 相似文献