全文获取类型
收费全文 | 320篇 |
免费 | 17篇 |
国内免费 | 76篇 |
出版年
2024年 | 1篇 |
2023年 | 7篇 |
2022年 | 10篇 |
2021年 | 11篇 |
2020年 | 10篇 |
2019年 | 6篇 |
2018年 | 6篇 |
2017年 | 8篇 |
2016年 | 7篇 |
2015年 | 8篇 |
2014年 | 15篇 |
2013年 | 18篇 |
2012年 | 5篇 |
2011年 | 13篇 |
2010年 | 9篇 |
2009年 | 27篇 |
2008年 | 16篇 |
2007年 | 15篇 |
2006年 | 20篇 |
2005年 | 23篇 |
2004年 | 18篇 |
2003年 | 22篇 |
2002年 | 20篇 |
2001年 | 10篇 |
2000年 | 7篇 |
1999年 | 10篇 |
1998年 | 12篇 |
1997年 | 3篇 |
1996年 | 5篇 |
1995年 | 4篇 |
1994年 | 6篇 |
1993年 | 8篇 |
1992年 | 4篇 |
1991年 | 5篇 |
1990年 | 2篇 |
1989年 | 6篇 |
1988年 | 6篇 |
1986年 | 3篇 |
1985年 | 7篇 |
1984年 | 4篇 |
1983年 | 1篇 |
1982年 | 3篇 |
1981年 | 1篇 |
1980年 | 1篇 |
1979年 | 3篇 |
1977年 | 1篇 |
1976年 | 2篇 |
1975年 | 1篇 |
1974年 | 3篇 |
排序方式: 共有413条查询结果,搜索用时 15 毫秒
111.
Contamination of groundwater by chlorinated solvents such as carbon tetrachloride (CCl4) and chloroform (CHCl3) is a widespread problem. The cell exudates from the methanogen Methanosarcina thermophila are active in the degradation of CCl4 and CHCl3. This research was performed to characterize these exudates. Examination of the influence of pH indicated that activity was greater under alkaline conditions. Rapid CCl4 degradation occurred from 35-65 degrees C, with first-order degradation rate coefficients increasing as temperature increased. It was found that proteins were not responsible for CCl4 degradation. The active agents in the cell exudates were <10 kDa in size, with degradation activity present in both 1-10 kDa and <1 kDa size ranges. Upon purification of the <10 kDa size range of the cell exudates on a C(18) chromatography column, 17 fractions (out of 100) degraded >50% of the added CCl4 in 8 h. These 17 fractions were pooled into three samples based on their elution time from the C(18) column. One of these pooled samples contained elevated levels of cobalt, zinc, and iron, at 2, 3, and 13 times the levels measured in similarly fractionated and pooled samples of medium, respectively. The UV-visible spectrum of this pooled sample had an absorption maximum at 560-580 nm, which is similar to the absorption maxima of heme (approximately 550 and 575 nm). The two other pooled samples contained elevated levels of zinc at 11 and 22 times the concentration measured in similarly fractionated and pooled samples of medium, respectively, and also contained very low levels of nickel, cobalt, and iron. This research suggests that the cell exudates from M. thermophila contain porphorinogen-type molecules capable of dechlorination, possibly excreted corrinoids, hemes, and zinc-containing molecules. 相似文献
112.
Mechanical properties of the topsoil (sandy Podsol and silty Luvisol, FAO) adhering to maize (Zea mays L.) roots and its bulk soil counterpart were studied as a function of soil texture and final soil water suction at harvest,
with three soil water suction values of approximately 30, 50 and 60 kPa. Two scales of observation were also selected: the
whole soil:root system and the root-adhering soil aggregates. Three methods were used to characterize the stability of the
soil:root system: mechanical shaking in air, and dispersion by low-power ultrasonication, with or without preliminary immersion
of the soil:root system in water. Soil disruption kinetics, which were fitted with first-order kinetics equations, were analyzed
and discussed. For example, silty soil ultrasonication kinetics, without preliminary water-immersion, could be divided into
two parts: the first faster part, which was characterized by a mean rate K value of 6.8–7.2 mJ-1, is attributed to soil slaking, whereas the second slower part, which was characterized by a mean rate K value of 1.5–1.6 mJ-1, was attributed to the rupture of the `firmly root-adhering soil' from the roots. A clear plant effect was observed for both
aggregate tensile strength and friability, with higher aggregate strength for the root-adhering silty soil (450–500 kPa) than
for its bulk silty soil counterpart (410–420 kPa), and lower friability (coefficient of variation of the aggregate strength)
for the root-adhering silty soil (e.g. 67% at a soil water suction value of 30 kPa) than for its bulk silty soil counterpart
(e.g. 49% at asoil water suction value of 30 kPa). These effects were attributed to root exudation, which was significantly
higher for the driest silty topsoil than for the wetter ones. In conclusion, the mechanical properties of the silty topsoil
adhering to the maize roots are attributed to both physical and biological interactions occurring in the maize rhizosphere.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
113.
《Bioscience, biotechnology, and biochemistry》2013,77(9):1500-1502
In the xylem exudate extracted from the current-year stems of apple (Malus domestica Borkh.), gibberellins A15, A17, A18, A19, A23, A44, and A53 were identified, and 16,17-dihydro-17-hydroxy GA19 was presumed from full-scan mass spectra and Kovats retention indices. 相似文献
114.
Yoshihito Hongo 《Journal of Ethology》2006,24(3):201-204
The Japanese horned beetle, Trypoxylus dichotomus septentrionalis, usually aggregates at the wounds caused by boring insects on Quercus oak trees to feed on exuding sap. Here, a unique behavior of the beetle, which enables it to independently obtain sap without any activities of other insects, is reported. Beetles carved intact bark of Fraxinus griffithii, where no sap was exuding, using clypeus. As a result of carving behavior, bark was stripped, and sap exuded from these scars temporarily, which beetles immediately began to lick. A digital video image relating to the article is available at (). 相似文献
115.
Summary One of the factors giving rise to soil sickness in apple orchards is the rhizosphere microflora. The composition of the microbial
coenosis in the rhizosphere changes with increasing age of the apple trees. An increase in the counts of micromycetes and
actinomycetes and a decrease in bacterial counts was found in agreement with the decreasing pH of the rhizosphere soil. The
presence of fluorescent pseudomonads in the rhizosphere of old apple trees was rare, but the planting of apple seedling into
sick soil induced their proliferation. The relative proportion of individual genera of micromycetes changed according to the
tree age; fungi of the genus Mucor were more often found in the rhizosphere of younger trees than in that of older ones while
fungi of the genus Penicillium had an opposite trend. Biological tests showed that Penicillium fungi form the majority of
the phytotoxic microflora. The amount of phytotoxic micromycetes was higher in ‘sick’ soil as compared with control soil in
which apple trees had not been grown for at least 15 years. Higher numbers of phytotoxic micromycetes were isolated also from
the rhizosphere of apple seedlings grown in ‘sick’ soil as compared with those growing in control soil. An increase in the
amount of phytotoxic micromycetes in apple tree rhizosphere could be induced by mere addition of 5% (w/w) ‘sick’ soil to the
soil in which apple trees were grown for the first time. By adding sterilized ‘sick’ soil, the amount of phytotoxic micromycetes
in the apple seedling rhizosphere was not affected. Increased numbers of phytotoxic micromycetes affected negatively the growth
of apple trees and the morphology of apple tree roots. This demonstrated the possibility of transfer of a factor participating
in the etiology of soil sickness in apple orchards. 相似文献
116.
Background and AimsAn increase in root hair length and density and the development of arbuscular mycorrhiza symbiosis are two alternative strategies of most plants to increase the root–soil surface area under phosphorus (P) deficiency. Across many plant species, root hair length and mycorrhization density are inversely correlated. Root architecture, rooting density and physiology also differ between species. This study aims to understand the relationship among root hairs, arbuscular mycorrhizal fungi (AMF) colonization, plant growth, P acquisition and mycorrhizal-specific Pi transporter gene expression in maize.MethodsUsing nearly isogenic maize lines, the B73 wild type and the rth3 root hairless mutant, we quantified the effect of root hairs and AMF infection in a calcareous soil under P deficiency through a combined analysis of morphological, physiological and molecular factors.Key ResultsWild-type root hairs extended the rhizosphere for acid phosphatase activity by 0.5 mm compared with the rth3 hairless mutant, as measured by in situ zymography. Total root length of the wild type was longer than that of rth3 under P deficiency. Higher AMF colonization and mycorrhiza-induced phosphate transporter gene expression were identified in the mutant under P deficiency, but plant growth and P acquisition were similar between mutant and the wild type. The mycorrhizal dependency of maize was 33 % higher than the root hair dependency.ConclusionsThe results identified larger mycorrhizal dependency than root hair dependency under P deficiency in maize. Root hairs and AMF inoculation are two alternative ways to increase Pi acquisition under P deficiency, but these two strategies compete with each other. 相似文献
117.
自然成土过程中土壤养分的变化与植被原生演替常同时发生。随成土年龄变化的植物养分捕获策略(NASs)对植物竞争能力和演替过程具有重要影响。该文将植物NASs划分为细根、微生物、特殊根系、食虫和寄生策略等5个类型; 发现植物NASs的多样性随成土年龄的增加呈哑铃型变化模式; 特殊根系策略对植物捕获养分的作用在成土中期最小、后期最大, 细根和微生物策略的作用随成土年龄的增加逐渐降低; 分析了成土过程中NASs对植物种间关系影响的变化, 发现NASs对成土早期植物的促进作用和中期的竞争关系具有重要影响, 而成土后期多样和互补的NASs对植被群落的稳定共存及多样性的形成具有影响; 提出应进一步探究成土过程中土壤养分与植物NASs变化之间的定量关系, 开展更多研究以阐明NASs对植被原生演替、物种多样性形成和成土过程的贡献与机理。 相似文献
118.
119.
Peng Mao Meng Zhao Fan Zhang Yapeng Fang Glyn O. Phillips Katsuyoshi Nishinari Fatang Jiang 《Carbohydrate polymers》2013
This paper investigates the phase separation and phase separation-induced fractionation of gum arabic (GA)/sugar beet pectin (SBP) mixed solutions. A phase diagram, including cloud and binodal curves, was established by visual observation and phase composition analysis. The deviation of the binodal curve from the cloud curve was a result of phase separation-induced fractionation of polydisperse GA and SBP molecules. Fractionation of GA increased the content of arabinogalactan-protein complex (AGP) from ca. 13% to 27%. The fractionated GA (FGA) showed improved emulsifying functionality, whereas the fractionated SBP (FSBP) had a reduced emulsifying functionality. The changes in emulsifying efficiency can be explained by interfacial adsorption behaviors at the oil–water interface as indicated by interfacial tension measurements. 相似文献
120.
Biodegradation studies of Gum ghatti (Gg) and acrylamide-co-acrylic acid based flocculants [Gg-cl-poly(AAm-co-AA)] have been reported using the soil composting method. Gg-cl-poly(AAm-co-AA) was found to degrade 89.76% within 60 days. The progress of biodegradation at each stage was monitored through FT-IR and SEM. Polymer was synthesized under pressure using potassium persulphate-ascorbic acid as a redox initiator and N,N′-methylene-bis-acrylamide as a crosslinker. Synthesized polymer was found to show pH, temperature and ionic strength of the cations dependent swelling behavior. Gg-cl-poly(AAm-co-AA) was utilized for the selective absorption of saline from different petroleum fraction-saline emulsions. The flocculation efficiency of the polymer was studied as a function of polymer dose, temperature and pH of the solution. Gg-cl-poly(AAm-co-AA) showed maximum flocculation efficiency with 20 mol L−1 polymer dose in acidic medium at 50 °C. 相似文献